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P04000

- OPSR_HUMAN

UniProt

P04000 - OPSR_HUMAN

Protein

Long-wave-sensitive opsin 1

Gene

OPN1LW

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 152 (01 Oct 2014)
      Sequence version 1 (23 Oct 1986)
      Previous versions | rss
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    Functioni

    Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.

    Absorptioni

    Abs(max)=560 nm

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW
    2. photoreceptor activity Source: ProtInc

    GO - Biological processi

    1. phototransduction, visible light Source: Reactome
    2. positive regulation of cytokinesis Source: UniProt
    3. protein-chromophore linkage Source: UniProtKB-KW
    4. retinoid metabolic process Source: Reactome
    5. signal transduction Source: ProtInc
    6. visual perception Source: ProtInc

    Keywords - Molecular functioni

    G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    Chromophore

    Enzyme and pathway databases

    ReactomeiREACT_160083. The retinoid cycle in cones (daylight vision).
    REACT_160130. Retinoid cycle disease events.
    REACT_18426. Opsins.
    REACT_19231. G alpha (i) signalling events.

    Protein family/group databases

    TCDBi9.A.14.1.1. the g-protein-coupled receptor (gpcr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long-wave-sensitive opsin 1
    Alternative name(s):
    Red cone photoreceptor pigment
    Red-sensitive opsin
    Short name:
    ROP
    Gene namesi
    Name:OPN1LW
    Synonyms:RCP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome X

    Organism-specific databases

    HGNCiHGNC:9936. OPN1LW.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: ProtInc
    2. photoreceptor outer segment membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Colorblindness, partial, protan series (CBP) [MIM:303900]: A color vision defect characterized by a dichromasy in which red and green are confused, with loss of luminance and shift of brightness and hue curves toward the short wave end of the spectrum. Dichromasy is due to the use of only two types of photoreceptors, blue plus red in deuteranopia and blue plus green in protanopia.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti338 – 3381G → E in CBP. 1 Publication
    VAR_064054
    Blue cone monochromacy (BCM) [MIM:303700]: A rare X-linked congenital stationary cone dysfunction syndrome characterized by the absence of functional long wavelength-sensitive and medium wavelength-sensitive cones in the retina. Color discrimination is severely impaired from birth, and vision is derived from the remaining preserved blue (S) cones and rod photoreceptors. BCM typically presents with reduced visual acuity, pendular nystagmus, and photophobia. Patients often have myopia.2 Publications
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti203 – 2031C → R in BCM. 2 Publications
    VAR_009298
    Natural varianti307 – 3071P → L in BCM. 1 Publication
    VAR_009299

    Keywords - Diseasei

    Disease mutation

    Organism-specific databases

    MIMi303700. phenotype.
    303900. phenotype.
    Orphaneti16. Blue cone monochromatism.
    1872. Cone rod dystrophy.
    319691. Partial color blindness, protan type.
    PharmGKBiPA31936.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 364364Long-wave-sensitive opsin 1PRO_0000197802Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi34 – 341N-linked (GlcNAc...)Curated
    Disulfide bondi126 ↔ 203PROSITE-ProRule annotation
    Modified residuei312 – 3121N6-(retinylidene)lysine

    Post-translational modificationi

    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP04000.
    PRIDEiP04000.

    Expressioni

    Tissue specificityi

    The three color pigments are found in the cone photoreceptor cells.

    Gene expression databases

    BgeeiP04000.
    CleanExiHS_OPN1LW.
    GenevestigatoriP04000.

    Interactioni

    Protein-protein interaction databases

    BioGridi111889. 1 interaction.
    STRINGi9606.ENSP00000358967.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KPXmodel-A1-364[»]
    ProteinModelPortaliP04000.
    SMRiP04000. Positions 23-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5252ExtracellularAdd
    BLAST
    Topological domaini78 – 8912CytoplasmicAdd
    BLAST
    Topological domaini116 – 12914ExtracellularAdd
    BLAST
    Topological domaini150 – 16819CytoplasmicAdd
    BLAST
    Topological domaini193 – 21826ExtracellularAdd
    BLAST
    Topological domaini247 – 26822CytoplasmicAdd
    BLAST
    Topological domaini293 – 3008Extracellular
    Topological domaini326 – 36439CytoplasmicAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei53 – 7725Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei90 – 11526Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei130 – 14920Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei169 – 19224Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei219 – 24628Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei269 – 29224Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei301 – 32525Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG240324.
    HOGENOMiHOG000253932.
    HOVERGENiHBG107442.
    InParanoidiP04000.
    OMAiMAEEWGK.
    OrthoDBiEOG72NRQJ.
    PhylomeDBiP04000.
    TreeFamiTF324998.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001760. Opsin.
    IPR000378. Opsin_red/grn.
    IPR027430. Retinal_BS.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00238. OPSIN.
    PR00575. OPSINREDGRN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    PS00238. OPSIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04000-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQQWSLQRL AGRHPQDSYE DSTQSSIFTY TNSNSTRGPF EGPNYHIAPR    50
    WVYHLTSVWM IFVVTASVFT NGLVLAATMK FKKLRHPLNW ILVNLAVADL 100
    AETVIASTIS IVNQVSGYFV LGHPMCVLEG YTVSLCGITG LWSLAIISWE 150
    RWLVVCKPFG NVRFDAKLAI VGIAFSWIWS AVWTAPPIFG WSRYWPHGLK 200
    TSCGPDVFSG SSYPGVQSYM IVLMVTCCII PLAIIMLCYL QVWLAIRAVA 250
    KQQKESESTQ KAEKEVTRMV VVMIFAYCVC WGPYTFFACF AAANPGYAFH 300
    PLMAALPAYF AKSATIYNPV IYVFMNRQFR NCILQLFGKK VDDGSELSSA 350
    SKTEVSSVSS VSPA 364
    Length:364
    Mass (Da):40,572
    Last modified:October 23, 1986 - v1
    Checksum:iD9DACDC17BF20DB3
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti65 – 651T → I.
    Corresponds to variant rs1065419 [ dbSNP | Ensembl ].
    VAR_012009
    Natural varianti111 – 1111I → V.
    Corresponds to variant rs1065421 [ dbSNP | Ensembl ].
    VAR_012010
    Natural varianti116 – 1161S → Y.
    Corresponds to variant rs1065422 [ dbSNP | Ensembl ].
    VAR_012011
    Natural varianti153 – 1531L → M.
    Corresponds to variant rs713 [ dbSNP | Ensembl ].
    VAR_012012
    Natural varianti174 – 1741A → V.
    Corresponds to variant rs731613 [ dbSNP | Ensembl ].
    VAR_012013
    Natural varianti180 – 1801S → A in 38% of the population. 1 Publication
    Corresponds to variant rs949431 [ dbSNP | Ensembl ].
    VAR_004842
    Natural varianti203 – 2031C → R in BCM. 2 Publications
    VAR_009298
    Natural varianti230 – 2301I → T.
    Corresponds to variant rs1065425 [ dbSNP | Ensembl ].
    VAR_012014
    Natural varianti274 – 2741I → V.
    Corresponds to variant rs2315122 [ dbSNP | Ensembl ].
    VAR_050612
    Natural varianti298 – 2981A → P.
    Corresponds to variant rs1065440 [ dbSNP | Ensembl ].
    VAR_012015
    Natural varianti307 – 3071P → L in BCM. 1 Publication
    VAR_009299
    Natural varianti309 – 3091Y → F.
    Corresponds to variant rs1065441 [ dbSNP | Ensembl ].
    VAR_012016
    Natural varianti338 – 3381G → E in CBP. 1 Publication
    VAR_064054

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13305
    , M13300, M13301, M13302, M13303, M13304 Genomic DNA. Translation: AAB59524.1.
    Z68193 Genomic DNA. Translation: CAA92342.1.
    CCDSiCCDS14742.1.
    PIRiA03157. OOHUR.
    UniGeneiHs.247787.
    Hs.592247.

    Genome annotation databases

    EnsembliENST00000369951; ENSP00000358967; ENSG00000102076.
    UCSCiuc004fjz.4. human.

    Polymorphism databases

    DMDMi129219.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Mutations of the color pigment genes

    Retina International's Scientific Newsletter

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13305
    , M13300 , M13301 , M13302 , M13303 , M13304 Genomic DNA. Translation: AAB59524.1 .
    Z68193 Genomic DNA. Translation: CAA92342.1 .
    CCDSi CCDS14742.1.
    PIRi A03157. OOHUR.
    UniGenei Hs.247787.
    Hs.592247.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KPX model - A 1-364 [» ]
    ProteinModelPortali P04000.
    SMRi P04000. Positions 23-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111889. 1 interaction.
    STRINGi 9606.ENSP00000358967.

    Chemistry

    ChEMBLi CHEMBL1949482.

    Protein family/group databases

    TCDBi 9.A.14.1.1. the g-protein-coupled receptor (gpcr) family.
    GPCRDBi Search...

    Polymorphism databases

    DMDMi 129219.

    Proteomic databases

    PaxDbi P04000.
    PRIDEi P04000.

    Protocols and materials databases

    DNASUi 5956.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000369951 ; ENSP00000358967 ; ENSG00000102076 .
    UCSCi uc004fjz.4. human.

    Organism-specific databases

    GeneCardsi GC0XP153409.
    GeneReviewsi OPN1LW.
    H-InvDB HIX0203334.
    HGNCi HGNC:9936. OPN1LW.
    MIMi 300822. gene.
    303700. phenotype.
    303900. phenotype.
    neXtProti NX_P04000.
    Orphaneti 16. Blue cone monochromatism.
    1872. Cone rod dystrophy.
    319691. Partial color blindness, protan type.
    PharmGKBi PA31936.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG240324.
    HOGENOMi HOG000253932.
    HOVERGENi HBG107442.
    InParanoidi P04000.
    OMAi MAEEWGK.
    OrthoDBi EOG72NRQJ.
    PhylomeDBi P04000.
    TreeFami TF324998.

    Enzyme and pathway databases

    Reactomei REACT_160083. The retinoid cycle in cones (daylight vision).
    REACT_160130. Retinoid cycle disease events.
    REACT_18426. Opsins.
    REACT_19231. G alpha (i) signalling events.

    Miscellaneous databases

    ChiTaRSi OPN1LW. human.
    NextBioi 23196.
    PROi P04000.
    SOURCEi Search...

    Gene expression databases

    Bgeei P04000.
    CleanExi HS_OPN1LW.
    Genevestigatori P04000.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001760. Opsin.
    IPR000378. Opsin_red/grn.
    IPR027430. Retinal_BS.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00238. OPSIN.
    PR00575. OPSINREDGRN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    PS00238. OPSIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular genetics of human color vision: the genes encoding blue, green, and red pigments."
      Nathans J., Thomas D., Hogness D.S.
      Science 232:193-202(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Retinal cone cell.
    2. "The DNA sequence of the human X chromosome."
      Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
      , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
      Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Molecular biology of the visual pigments."
      Applebury M.L., Hargrave P.A.
      Vision Res. 26:1881-1895(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    4. "Polymorphism in red photopigment underlies variation in colour matching."
      Winderickx J., Lindsey D.T., Sanocki E., Teller D.Y., Motulsky A.G., Deeb S.S.
      Nature 356:431-433(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT ALA-180.
    5. "Genetic heterogeneity among blue-cone monochromats."
      Nathans J., Maumenee I.H., Zrenner E., Sadowski B., Sharpe L.T., Lewis R.A., Hansen E., Rosenberg T., Schwartz M., Heckenlively J.R.
      Am. J. Hum. Genet. 53:987-1000(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS BCM ARG-203 AND LEU-307.
    6. "Gene conversion between red and defective green opsin gene in blue cone monochromacy."
      Reyniers E., Van Thienen M.N., Meire F., De Boulle K., Devries K., Kestelijn P., Willems P.J.
      Genomics 29:323-328(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT BCM ARG-203.
    7. "Novel missense mutations in red/green opsin genes in congenital color-vision deficiencies."
      Ueyama H., Kuwayama S., Imai H., Tanabe S., Oda S., Nishida Y., Wada A., Shichida Y., Yamade S.
      Biochem. Biophys. Res. Commun. 294:205-209(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT CBP GLU-338.

    Entry informationi

    Entry nameiOPSR_HUMAN
    AccessioniPrimary (citable) accession number: P04000
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 1986
    Last sequence update: October 23, 1986
    Last modified: October 1, 2014
    This is version 152 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3