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Protein

HLA class I histocompatibility antigen, B-27 alpha chain

Gene

HLA-B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the presentation of foreign antigens to the immune system.

GO - Molecular functioni

  • peptide antigen binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Immunity

Enzyme and pathway databases

ReactomeiR-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236977. Endosomal/Vacuolar pathway.
R-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-HSA-877300. Interferon gamma signaling.
R-HSA-909733. Interferon alpha/beta signaling.
R-HSA-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
HLA class I histocompatibility antigen, B-27 alpha chain
Alternative name(s):
MHC class I antigen B*27
Gene namesi
Name:HLA-B
Synonyms:HLAB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:4932. HLA-B.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 308ExtracellularSequence analysisAdd BLAST284
Transmembranei309 – 332HelicalSequence analysisAdd BLAST24
Topological domaini333 – 362CytoplasmicSequence analysisAdd BLAST30

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • early endosome membrane Source: Reactome
  • endoplasmic reticulum Source: UniProtKB
  • ER to Golgi transport vesicle membrane Source: Reactome
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: Reactome
  • integral component of lumenal side of endoplasmic reticulum membrane Source: Reactome
  • membrane Source: UniProtKB
  • MHC class I protein complex Source: UniProtKB
  • phagocytic vesicle membrane Source: Reactome
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane, MHC I

Pathology & Biotechi

Involvement in diseasei

Spondyloarthropathy 1 (SPDA1)
Disease susceptibility is associated with variations affecting the gene represented in this entry. A restricted number of HLA-B27 subtypes can be associated with ankylosing spondylitis and other B27-related diseases, and an elevated frequency of the B*2702 allele in ankylosing spondylitis patients is identified. The allele B*2707 seems to have a protective role some populations because it was found only in the healthy controls.
Disease descriptionA chronic rheumatic disease with multifactorial inheritance. It includes a spectrum of related disorders comprising ankylosing spondylitis, a subset of psoriatic arthritis, reactive arthritis (e.g. Reiter syndrome), arthritis associated with inflammatory bowel disease and undifferentiated spondyloarthropathy. These disorders may occur simultaneously or sequentially in the same patient, probably representing various phenotypic expressions of the same disease. Ankylosing spondylitis is the form of rheumatoid arthritis affecting the spine and is considered the prototype of seronegative spondyloarthropathies. It produces pain and stiffness as a result of inflammation of the sacroiliac, intervertebral, and costovertebral joints.
See also OMIM:106300

Organism-specific databases

MalaCardsiHLA-B.
MIMi106300. phenotype.

Polymorphism and mutation databases

DMDMi34305677.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000001883925 – 362HLA class I histocompatibility antigen, B-27 alpha chainAdd BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi110N-linked (GlcNAc...)1 Publication1
Disulfide bondi125 ↔ 188
Disulfide bondi227 ↔ 283

Post-translational modificationi

Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

EPDiP03989.
MaxQBiP03989.
PeptideAtlasiP03989.
PRIDEiP03989.

PTM databases

SwissPalmiP03989.

Expressioni

Gene expression databases

BgeeiENSG00000224608.
CleanExiHS_HLA-B.

Interactioni

Subunit structurei

Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-6188N.
IntActiP03989. 1 interactor.
MINTiMINT-247525.

Structurei

Secondary structure

1362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 36Combined sources10
Beta strandi41 – 43Combined sources3
Beta strandi45 – 52Combined sources8
Beta strandi55 – 61Combined sources7
Beta strandi64 – 66Combined sources3
Helixi74 – 76Combined sources3
Helixi81 – 108Combined sources28
Beta strandi113 – 115Combined sources3
Beta strandi118 – 127Combined sources10
Beta strandi133 – 142Combined sources10
Beta strandi145 – 150Combined sources6
Beta strandi157 – 161Combined sources5
Helixi162 – 173Combined sources12
Helixi176 – 185Combined sources10
Helixi187 – 198Combined sources12
Turni199 – 204Combined sources6
Beta strandi210 – 219Combined sources10
Beta strandi222 – 235Combined sources14
Beta strandi238 – 243Combined sources6
Helixi249 – 251Combined sources3
Beta strandi252 – 254Combined sources3
Beta strandi261 – 263Combined sources3
Beta strandi265 – 274Combined sources10
Helixi278 – 280Combined sources3
Beta strandi281 – 286Combined sources6
Beta strandi290 – 292Combined sources3
Beta strandi294 – 296Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HSAX-ray2.10A/D25-300[»]
1JGDX-ray1.90A25-300[»]
1JGEX-ray2.10A25-300[»]
1K5NX-ray1.09A25-300[»]
1OF2X-ray2.20A25-300[»]
1OGTX-ray1.47A25-300[»]
1ROGmodel-A25-206[»]
1ROHmodel-A25-206[»]
1ROImodel-A25-206[»]
1ROJmodel-A25-206[»]
1ROKmodel-A25-206[»]
1ROLmodel-A25-206[»]
1UXSX-ray1.55A25-300[»]
1UXWX-ray1.71A25-300[»]
1W0VX-ray2.27A25-300[»]
1W0WX-ray2.10A25-300[»]
2A83X-ray1.40A25-300[»]
2BSRX-ray2.30A25-300[»]
2BSSX-ray2.00A25-300[»]
2BSTX-ray2.10A25-300[»]
3B3IX-ray1.86A25-300[»]
3B6SX-ray1.80A25-300[»]
3BP4X-ray1.85A25-300[»]
3BP7X-ray1.80A25-300[»]
3CZFX-ray1.20A25-300[»]
3D18X-ray1.74A25-300[»]
3DTXX-ray2.10A25-300[»]
3HCVX-ray1.95A25-300[»]
3LV3X-ray1.94A25-300[»]
4G8GX-ray2.40A25-300[»]
4G8IX-ray1.60A25-300[»]
4G9DX-ray1.60A25-300[»]
4G9FX-ray1.90A25-300[»]
5DEFX-ray1.60A25-300[»]
5DEGX-ray1.83A25-300[»]
ProteinModelPortaliP03989.
SMRiP03989.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03989.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini209 – 295Ig-like C1-typeAdd BLAST87

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 114Alpha-1Add BLAST90
Regioni115 – 206Alpha-2Add BLAST92
Regioni207 – 298Alpha-3Add BLAST92
Regioni299 – 308Connecting peptide10

Sequence similaritiesi

Belongs to the MHC class I family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG016709.
OrthoDBiEOG091G09OH.
PhylomeDBiP03989.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSiPR01638. MHCCLASSI.
SMARTiSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVTAPRTLL LLLWGAVALT ETWAGSHSMR YFHTSVSRPG RGEPRFITVG
60 70 80 90 100
YVDDTLFVRF DSDAASPREE PRAPWIEQEG PEYWDRETQI CKAKAQTDRE
110 120 130 140 150
DLRTLLRYYN QSEAGSHTLQ NMYGCDVGPD GRLLRGYHQD AYDGKDYIAL
160 170 180 190 200
NEDLSSWTAA DTAAQITQRK WEAARVAEQL RAYLEGECVE WLRRYLENGK
210 220 230 240 250
ETLQRADPPK THVTHHPISD HEATLRCWAL GFYPAEITLT WQRDGEDQTQ
260 270 280 290 300
DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP
310 320 330 340 350
SSQSTVPIVG IVAGLAVLAV VVIGAVVAAV MCRRKSSGGK GGSYSQAACS
360
DSAQGSDVSL TA
Length:362
Mass (Da):40,428
Last modified:August 13, 1987 - v2
Checksum:iC8D2F154E3292031
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti206A → V (PubMed:3485286).Curated1
Sequence conflicti266Q → E AA sequence (PubMed:2408663).Curated1

Polymorphismi

The following alleles of B-27 are known: B*27:01=B*27:05, B*27:02 (B27.2; B-27k; B27e), B*27:03 (B27d), B*27:04, B*27:06, B*27:07, B*27:08 (B7Qui) and B*27:09 (B27-ci). The sequence shown is that of B*27:01.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05634165A → T.Combined sourcesCorresponds to variant rs1050529dbSNPEnsembl.1
Natural variantiVAR_01637983Y → H in allele B*27:03. 1
Natural variantiVAR_016380101D → N in allele B*27:02. 1
Natural variantiVAR_016381101D → S in allele B*27:04, allele B*27:06 and allele B*27:08; requires 2 nucleotide substitutions. 1
Natural variantiVAR_016382104 – 105TL → IA in allele B*27:02. 2
Natural variantiVAR_016383104T → N in allele B*27:08. 1
Natural variantiVAR_016384106 – 107LR → RG in allele B*27:08. 2
Natural variantiVAR_016385121N → S in allele B*27:07. 1
Natural variantiVAR_016386137 – 138YH → HN in allele B*27:07. 2
Natural variantiVAR_016387138H → D in allele B*27:06. 1
Natural variantiVAR_016388140D → H in allele B*27:09. 1
Natural variantiVAR_016389140D → Y in allele B*27:06 and allele B*27:07. 1
Natural variantiVAR_016390155S → R in allele B*27:07. 1
Natural variantiVAR_016391176V → E in allele B*27:04 and allele B*27:06. 1
Natural variantiVAR_016392235A → G in allele B*27:04 and allele B*27:06. 1
Natural variantiVAR_056342306V → I.Corresponds to variant rs1131500dbSNPEnsembl.1
Natural variantiVAR_056343329A → T.Corresponds to variant rs1051488dbSNPEnsembl.1
Natural variantiVAR_061402349C → S.Corresponds to variant rs2308655dbSNPEnsembl.1
Natural variantiVAR_061403349C → Y.Corresponds to variant rs2308655dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03945 Genomic DNA. Translation: CAA27578.1. Sequence problems.
X03664, X03667 Genomic DNA. Translation: CAA27301.1.
L38504 mRNA. Translation: AAA69724.1.
M54883 Genomic DNA. Translation: AAA59616.1.
X03665, X03666 Genomic DNA. Translation: CAA27302.1.
M12967 Genomic DNA. Translation: AAA36221.1.
U27608 mRNA. Translation: AAC50444.1.
U35734 mRNA. Translation: AAC50447.1.
M14013 Genomic DNA. Translation: AAA59643.1.
X73578 mRNA. Translation: CAA51980.1.
M62852 mRNA. Translation: AAA59647.1.
L19923 mRNA. Translation: AAA59658.1.
Z33453 mRNA. Translation: CAA83876.1.
PIRiI37515.
I56116.
S07441. HLHUB2.
UniGeneiHs.654404.
Hs.726974.
Hs.77961.

Genome annotation databases

EnsembliENST00000435618; ENSP00000405178; ENSG00000224608.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03945 Genomic DNA. Translation: CAA27578.1. Sequence problems.
X03664, X03667 Genomic DNA. Translation: CAA27301.1.
L38504 mRNA. Translation: AAA69724.1.
M54883 Genomic DNA. Translation: AAA59616.1.
X03665, X03666 Genomic DNA. Translation: CAA27302.1.
M12967 Genomic DNA. Translation: AAA36221.1.
U27608 mRNA. Translation: AAC50444.1.
U35734 mRNA. Translation: AAC50447.1.
M14013 Genomic DNA. Translation: AAA59643.1.
X73578 mRNA. Translation: CAA51980.1.
M62852 mRNA. Translation: AAA59647.1.
L19923 mRNA. Translation: AAA59658.1.
Z33453 mRNA. Translation: CAA83876.1.
PIRiI37515.
I56116.
S07441. HLHUB2.
UniGeneiHs.654404.
Hs.726974.
Hs.77961.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HSAX-ray2.10A/D25-300[»]
1JGDX-ray1.90A25-300[»]
1JGEX-ray2.10A25-300[»]
1K5NX-ray1.09A25-300[»]
1OF2X-ray2.20A25-300[»]
1OGTX-ray1.47A25-300[»]
1ROGmodel-A25-206[»]
1ROHmodel-A25-206[»]
1ROImodel-A25-206[»]
1ROJmodel-A25-206[»]
1ROKmodel-A25-206[»]
1ROLmodel-A25-206[»]
1UXSX-ray1.55A25-300[»]
1UXWX-ray1.71A25-300[»]
1W0VX-ray2.27A25-300[»]
1W0WX-ray2.10A25-300[»]
2A83X-ray1.40A25-300[»]
2BSRX-ray2.30A25-300[»]
2BSSX-ray2.00A25-300[»]
2BSTX-ray2.10A25-300[»]
3B3IX-ray1.86A25-300[»]
3B6SX-ray1.80A25-300[»]
3BP4X-ray1.85A25-300[»]
3BP7X-ray1.80A25-300[»]
3CZFX-ray1.20A25-300[»]
3D18X-ray1.74A25-300[»]
3DTXX-ray2.10A25-300[»]
3HCVX-ray1.95A25-300[»]
3LV3X-ray1.94A25-300[»]
4G8GX-ray2.40A25-300[»]
4G8IX-ray1.60A25-300[»]
4G9DX-ray1.60A25-300[»]
4G9FX-ray1.90A25-300[»]
5DEFX-ray1.60A25-300[»]
5DEGX-ray1.83A25-300[»]
ProteinModelPortaliP03989.
SMRiP03989.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6188N.
IntActiP03989. 1 interactor.
MINTiMINT-247525.

PTM databases

SwissPalmiP03989.

Polymorphism and mutation databases

DMDMi34305677.

Proteomic databases

EPDiP03989.
MaxQBiP03989.
PeptideAtlasiP03989.
PRIDEiP03989.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000435618; ENSP00000405178; ENSG00000224608.

Organism-specific databases

GeneCardsiHLA-B.
HGNCiHGNC:4932. HLA-B.
MalaCardsiHLA-B.
MIMi106300. phenotype.
142830. gene.
neXtProtiNX_P03989.
GenAtlasiSearch...

Phylogenomic databases

HOVERGENiHBG016709.
OrthoDBiEOG091G09OH.
PhylomeDBiP03989.

Enzyme and pathway databases

ReactomeiR-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236977. Endosomal/Vacuolar pathway.
R-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-HSA-877300. Interferon gamma signaling.
R-HSA-909733. Interferon alpha/beta signaling.
R-HSA-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

ChiTaRSiHLA-B. human.
EvolutionaryTraceiP03989.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000224608.
CleanExiHS_HLA-B.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSiPR01638. MHCCLASSI.
SMARTiSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei1B27_HUMAN
AccessioniPrimary (citable) accession number: P03989
Secondary accession number(s): O19692
, P10317, P10318, P19373, P30467, Q08136, Q29693, Q29846, Q29961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: August 13, 1987
Last modified: November 2, 2016
This is version 174 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.