P03987 (IGHG3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ig gamma-3 chain C region
Organism	Mus musculus (Mouse)
Taxonomic identifier	10090 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location

Isoform 1: Cell membrane; Single-pass membrane protein By similarity. Isoform 2: Secreted.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin C region Immunoglobulin domain Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	antigen binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P03987-1)

Also known as: Membrane bound;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P03987-2)

Also known as: Secreted;

The sequence of this isoform differs from the canonical sequence as follows:
     328-398: ELELNETCAEAQDGELDGLWTTITIFISLFLLSVCYSASVTLFKVKWIFSSVVQVKQTAIPDYRNMIGQGA → GK

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

‹1 – 398

›398

Ig gamma-3 chain C region

PRO_0000153590

Regions

Transmembrane

350 – 370

21

Helical; Potential

Topological domain

371 – 398

28

Cytoplasmic Potential

Region

1 – 97

97

CH1

Region

98 – 113

16

Hinge

Region

114 – 223

110

CH2

Region

224 – 327

104

CH3

Amino acid modifications

Glycosylation

179

1

N-linked (GlcNAc...) Ref.3

Glycosylation

322

1

N-linked (GlcNAc...) Potential

Glycosylation

332

1

N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence

328 – 398

71

ELELN…IGQGA → GK in isoform 2.

VSP_034613

Experimental info

Sequence conflict

329

1

L → M in CAA24767. Ref.2

Sequence conflict

333

1

E → G in CAA24767. Ref.2

Non-terminal residue

1

1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

398

Helix Strand Turn

Details...

Beta strand

3 – 8

6

Beta strand

21 – 24

4

Beta strand

26 – 29

4

Beta strand

31 – 34

4

Beta strand

37 – 40

4

Beta strand

61 – 70

10

Turn

71 – 76

6

Beta strand

79 – 85

7

Helix

86 – 88

3

Beta strand

90 – 96

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Membrane bound) [UniParc]. Last modified August 1, 1991. Version 2. Checksum: CF7F264B50A41B95	FASTA	398	43,929
	10 20 30 40 50 60 TTTAPSVYPL VPGCSDTSGS SVTLGCLVKG YFPEPVTVKW NYGALSSGVR TVSSVLQSGF 70 80 90 100 110 120 YSLSSLVTVP SSTWPSQTVI CNVAHPASKT ELIKRIEPRI PKPSTPPGSS CPPGNILGGP 130 140 150 160 170 180 SVFIFPPKPK DALMISLTPK VTCVVVDVSE DDPDVHVSWF VDNKEVHTAW TQPREAQYNS 190 200 210 220 230 240 TFRVVSALPI QHQDWMRGKE FKCKVNNKAL PAPIERTISK PKGRAQTPQV YTIPPPREQM 250 260 270 280 290 300 SKKKVSLTCL VTNFFSEAIS VEWERNGELE QDYKNTPPIL DSDGTYFLYS KLTVDTDSWL 310 320 330 340 350 360 QGEIFTCSVV HEALHNHHTQ KNLSRSPELE LNETCAEAQD GELDGLWTTI TIFISLFLLS 370 380 390 VCYSASVTLF KVKWIFSSVV QVKQTAIPDY RNMIGQGA
	Isoform 2 (Secreted) [UniParc]. Checksum: F45827174182BAD6	FASTA	329	36,228
	10 20 30 40 50 60 TTTAPSVYPL VPGCSDTSGS SVTLGCLVKG YFPEPVTVKW NYGALSSGVR TVSSVLQSGF 70 80 90 100 110 120 YSLSSLVTVP SSTWPSQTVI CNVAHPASKT ELIKRIEPRI PKPSTPPGSS CPPGNILGGP 130 140 150 160 170 180 SVFIFPPKPK DALMISLTPK VTCVVVDVSE DDPDVHVSWF VDNKEVHTAW TQPREAQYNS 190 200 210 220 230 240 TFRVVSALPI QHQDWMRGKE FKCKVNNKAL PAPIERTISK PKGRAQTPQV YTIPPPREQM 250 260 270 280 290 300 SKKKVSLTCL VTNFFSEAIS VEWERNGELE QDYKNTPPIL DSDGTYFLYS KLTVDTDSWL 310 320 QGEIFTCSVV HEALHNHHTQ KNLSRSPGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of the murine IgG3 constant region gene." Wels J.A., Word C.J., Rimm D., Der-Balan G.P., Martinez H.M., Tucker P.W., Blattner F.R. EMBO J. 3:2041-2046(1984) [PubMed: 6092053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c.
[2]	"The structure of the mouse immunoglobulin in gamma 3 membrane gene segment." Komaromy M., Clayton L., Rogers J., Robertson S., Kettman J., Wall R. Nucleic Acids Res. 11:6775-6785(1983) [PubMed: 6314258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 329-398.
[3]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed: 17330941] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-179, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J00451 Genomic DNA. Translation: AAB59655.1.
V01526 Genomic DNA. Translation: CAA24767.1. Sequence problems.

IPI

IPI00117022.
IPI00807983.

PIR

G3MSM. A02156.
G3MSC. B02156.

UniGene

Mm.342177.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CLZ	X-ray	2.80	H	1-99	[»]
2VQ1	X-ray	2.50	B/F	1-99	[»]

ProteinModelPortal

P03987.

SMR

P03987. Positions 1-325.

ModBase

Search...

Protein-protein interaction databases

STRING

P03987.

Protein family/group databases

IMGT

Search...
Search...

Proteomic databases

PRIDE

P03987.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Phylogenomic databases

GeneTree

ENSGT00530000063726.

HOGENOM

HBG505540.

HOVERGEN

HBG005814.

InParanoid

P03987.

OrthoDB

EOG4RXZ0H.

Gene expression databases

ArrayExpress

P03987.

Bgee

P03987.

Genevestigator

P03987.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 3 hits.

Pfam

PF07654. C1-set. 3 hits.
[Graphical view]

SMART

SM00407. IGc1. 2 hits.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 3 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IGHG3_MOUSE

Accession

Primary (citable) accession number: P03987
Secondary accession number(s): P22436

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
Last sequence update:	August 1, 1991
Last modified:	September 21, 2011
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program