Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P03973 (SLPI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 152. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Antileukoproteinase

Short name=ALP
Alternative name(s):
BLPI
HUSI-1
Mucus proteinase inhibitor
Short name=MPI
Protease inhibitor WAP4
Secretory leukocyte protease inhibitor
Seminal proteinase inhibitor
WAP four-disulfide core domain protein 4
Gene names
Name:SLPI
Synonyms:WAP4, WFDC4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length132 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.

Subcellular location

Secreted.

Tissue specificity

Mucous fluids.

Miscellaneous

The pathologies of several chronic and acute diseases of the respiratory tract involve an imbalance between the proteases of cells involved in inflammatary responses and the inhibitors of these proteases. The inflammation-mediated release of neutrophil elastase in the lungs of patients whose levels of active alpha-1-antiprotease are compromised by genetic background, cigarette smoking, air pollutants, or a combination of all three can result in severe lung damage and a decreased lifespan. The relatively small size of this protein, its lack of glycosylation and its stability make this protein a candidate for use as a therapeutic agent in diseases mediated by leukocyte elastase-antielastase imbalances.

Sequence similarities

Contains 2 WAP domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.8 Ref.9 Ref.10
Chain26 – 132107Antileukoproteinase
PRO_0000041355

Regions

Domain28 – 7649WAP 1
Domain82 – 13049WAP 2
Region26 – 8358Trypsin inhibitory domain
Region84 – 13249Elastase inhibitory domain

Sites

Site45 – 462Reactive bond for trypsin Probable
Site97 – 982Reactive bond for chymotrypsin and elastase Probable

Amino acid modifications

Disulfide bond35 ↔ 64
Disulfide bond43 ↔ 68
Disulfide bond51 ↔ 63
Disulfide bond57 ↔ 72
Disulfide bond89 ↔ 118
Disulfide bond96 ↔ 122
Disulfide bond105 ↔ 117
Disulfide bond111 ↔ 126

Secondary structure

........... 132
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P03973 [UniParc].

Last modified October 1, 1989. Version 2.
Checksum: B62F3221E0903D90

FASTA13214,326
        10         20         30         40         50         60 
MKSSGLFPFL VLLALGTLAP WAVEGSGKSF KAGVCPPKKS AQCLRYKKPE CQSDWQCPGK 

        70         80         90        100        110        120 
KRCCPDTCGI KCLDPVDTPN PTRRKPGKCP VTYGQCLMLN PPNFCEMDGQ CKRDLKCCMG 

       130 
MCGKSCVSPV KA 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of cDNA for human antileukoprotease from cervix uterus."
Heinzel R., Appelhans H., Gassen G., Seemueller U., Machleidt W., Fritz H., Steffens G.
Eur. J. Biochem. 160:61-67(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Cervix.
[2]"Isolation and sequence of a human gene encoding a potent inhibitor of leukocyte proteases."
Stetler G., Brewer M.T., Thompson R.C.
Nucleic Acids Res. 14:7883-7896(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Parotid gland.
[3]"Cloning and characterization of SLPI from human intestinal epithelium."
Si-Tahar M., Merlin D., Sitaraman S., Madara J.L.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Intestine.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Tongue.
[5]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[8]"The acid-stable proteinase inhibitor of human mucous secretions (HUSI-I, antileukoprotease). Complete amino acid sequence as revealed by protein and cDNA sequencing and structural homology to whey proteins and Red sea turtle proteinase inhibitor."
Seemueller U., Arnhold M., Fritz H., Wiedenmann K., Machleidt W., Heinzel R., Appelhans H., Gassen H.-G., Lottspeich F.
FEBS Lett. 199:43-48(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-132, NUCLEOTIDE SEQUENCE OF 26-65.
[9]"Isolation, properties, and complete amino acid sequence of human secretory leukocyte protease inhibitor, a potent inhibitor of leukocyte elastase."
Thompson R.C., Ohlsson K.
Proc. Natl. Acad. Sci. U.S.A. 83:6692-6696(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-132.
[10]"Purification and characterization of elastase-specific inhibitor. Sequence homology with mucus proteinase inhibitor."
Sallenave J.-M., Ryle A.P.
Biol. Chem. Hoppe-Seyler 372:13-21(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-52.
[11]"The 2.5 A X-ray crystal structure of the acid-stable proteinase inhibitor from human mucous secretions analysed in its complex with bovine alpha-chymotrypsin."
Gruetter M.G., Fendrich G., Huber R., Bode W.
EMBO J. 7:345-351(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04470 mRNA. Translation: CAA28158.1.
X04502 Genomic DNA. Translation: CAA28187.1.
X04503 mRNA. Translation: CAA28188.1.
AF114471 mRNA. Translation: AAD19661.1.
AK312192 mRNA. Translation: BAG35125.1.
AL035660 Genomic DNA. Translation: CAB64235.1.
CH471077 Genomic DNA. Translation: EAW75869.1.
BC020708 mRNA. Translation: AAH20708.1.
CCDSCCDS13347.1.
PIRTIHUSP. A25541.
RefSeqNP_003055.1. NM_003064.3.
UniGeneHs.517070.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2Z7FX-ray1.70I83-132[»]
4DOQX-ray2.00B/D85-131[»]
ProteinModelPortalP03973.
SMRP03973. Positions 83-132.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112475. 2 interactions.
STRING9606.ENSP00000342082.

Protein family/group databases

MEROPSI17.001.

Polymorphism databases

DMDM113636.

Proteomic databases

MaxQBP03973.
PaxDbP03973.
PeptideAtlasP03973.
PRIDEP03973.

Protocols and materials databases

DNASU6590.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000338380; ENSP00000342082; ENSG00000124107.
GeneID6590.
KEGGhsa:6590.
UCSCuc002xnm.1. human.

Organism-specific databases

CTD6590.
GeneCardsGC20M043880.
HGNCHGNC:11092. SLPI.
HPACAB002303.
HPA027774.
MIM107285. gene.
neXtProtNX_P03973.
PharmGKBPA35944.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45457.
HOGENOMHOG000115819.
HOVERGENHBG018073.
InParanoidP03973.
OMAPWAVEGS.
OrthoDBEOG7S7SG9.
PhylomeDBP03973.
TreeFamTF338375.

Gene expression databases

ArrayExpressP03973.
BgeeP03973.
CleanExHS_SLPI.
GenevestigatorP03973.

Family and domain databases

Gene3D4.10.75.10. 2 hits.
InterProIPR008197. WAP-type_4-diS_core.
[Graphical view]
PfamPF00095. WAP. 2 hits.
[Graphical view]
PRINTSPR00003. 4DISULPHCORE.
SMARTSM00217. WAP. 2 hits.
[Graphical view]
SUPFAMSSF57256. SSF57256. 2 hits.
PROSITEPS51390. WAP. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLPI. human.
EvolutionaryTraceP03973.
GeneWikiSLPI.
GenomeRNAi6590.
NextBio25637.
PMAP-CutDBP03973.
PROP03973.
SOURCESearch...

Entry information

Entry nameSLPI_HUMAN
AccessionPrimary (citable) accession number: P03973
Secondary accession number(s): B2R5H8, P07757
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: October 1, 1989
Last modified: July 9, 2014
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM