Reviewed,
UniProtKB/Swiss-Prot P03973 (SLPI_HUMAN)
Last modified
November 25, 2008.
Version 102.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Antileukoproteinase Short name=ALP Alternative name(s): Secretory leukocyte protease inhibitor HUSI-1 Seminal proteinase inhibitor BLPI Mucus proteinase inhibitor Short name=MPI WAP four-disulfide core domain protein 4 Protease inhibitor WAP4 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 132 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues. |
| Subcellular location | |
| Tissue specificity | Mucous fluids. |
| Involvement in disease | The pathologies of several chronic and acute diseases of the respiratory tract involve an imbalance between the proteases of cells involved in inflammatary responses and the inhibitors of these proteases. The inflammation-mediated release of neutrophil elastase in the lungs of patients whose levels of active alpha-1-antiprotease are compromised by genetic background, cigarette smoking, air pollutants, or a combination of all three can result in severe lung damage and a decreased lifespan. The relatively small size of this protein, its lack of glycosylation and its stability make this protein a candidate for use as a therapeutic agent in diseases mediated by leukocyte elastase-antielastase imbalances. |
| Sequence similarities | Contains 2 WAP domains. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Secreted |
| Domain | Repeat Signal |
| Molecular function | Protease inhibitor Serine protease inhibitor |
| Technical term | 3D-structure Direct protein sequencing |
Gene Ontology (GO) | |
| Cellular component | extracellular region Inferred from electronic annotation. Source: InterPro |
| Molecular function | serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 25 | 25 | |||||||||
| Chain | 26 – 132 | 107 | Antileukoproteinase | PRO_0000041355 | |||||||
Regions | |||||||||||
| Domain | 28 – 76 | 49 | WAP 1 | ||||||||
| Domain | 82 – 130 | 49 | WAP 2 | ||||||||
| Region | 26 – 83 | 58 | Trypsin inhibitory domain | ||||||||
| Region | 84 – 132 | 49 | Elastase inhibitory domain | ||||||||
Sites | |||||||||||
| Site | 45 – 46 | 2 | Reactive bond for trypsin Probable | ||||||||
| Site | 97 – 98 | 2 | Reactive bond for chymotrypsin and elastase Probable | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 35 ↔ 64 | ||||||||||
| Disulfide bond | 43 ↔ 68 | ||||||||||
| Disulfide bond | 51 ↔ 63 | ||||||||||
| Disulfide bond | 57 ↔ 72 | ||||||||||
| Disulfide bond | 89 ↔ 118 | ||||||||||
| Disulfide bond | 96 ↔ 122 | ||||||||||
| Disulfide bond | 105 ↔ 117 | ||||||||||
| Disulfide bond | 111 ↔ 126 | ||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning and expression of cDNA for human antileukoprotease from cervix uterus." Heinzel R., Appelhans H., Gassen G., Seemueller U., Machleidt W., Fritz H., Steffens G. Eur. J. Biochem. 160:61-67(1986) [PubMed: 3533531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cervix. |
| [2] | "Isolation and sequence of a human gene encoding a potent inhibitor of leukocyte proteases." Stetler G., Brewer M.T., Thompson R.C. Nucleic Acids Res. 14:7883-7896(1986) [PubMed: 3640338] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Parotid gland. |
| [3] | "Cloning and characterization of SLPI from human intestinal epithelium." Si-Tahar M., Merlin D., Sitaraman S., Madara J.L. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Intestine. |
| [4] | "The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. Rogers J.Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver. |
| [6] | "The acid-stable proteinase inhibitor of human mucous secretions (HUSI-I, antileukoprotease). Complete amino acid sequence as revealed by protein and cDNA sequencing and structural homology to whey proteins and Red sea turtle proteinase inhibitor." Seemueller U., Arnhold M., Fritz H., Wiedenmann K., Machleidt W., Heinzel R., Appelhans H., Gassen H.-G., Lottspeich F. FEBS Lett. 199:43-48(1986) [PubMed: 3485543] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-132, NUCLEOTIDE SEQUENCE OF 26-65. |
| [7] | "Isolation, properties, and complete amino acid sequence of human secretory leukocyte protease inhibitor, a potent inhibitor of leukocyte elastase." Thompson R.C., Ohlsson K. Proc. Natl. Acad. Sci. U.S.A. 83:6692-6696(1986) [PubMed: 3462719] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-132. |
| [8] | "Purification and characterization of elastase-specific inhibitor. Sequence homology with mucus proteinase inhibitor." Sallenave J.-M., Ryle A.P. Biol. Chem. Hoppe-Seyler 372:13-21(1991) [PubMed: 2039600] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-52. |
| [9] | "The 2.5 A X-ray crystal structure of the acid-stable proteinase inhibitor from human mucous secretions analysed in its complex with bovine alpha-chymotrypsin." Gruetter M.G., Fendrich G., Huber R., Bode W. EMBO J. 7:345-351(1988) [PubMed: 3366116] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X04470 mRNA. Translation: CAA28158.1. X04502 Genomic DNA. Translation: CAA28187.1. X04503 mRNA. Translation: CAA28188.1. AF114471 mRNA. Translation: AAD19661.1. AL035660 Genomic DNA. Translation: CAB64235.1. BC020708 mRNA. Translation: AAH20708.1. | |||||||||||||
| PIR | TIHUSP. A25541. | ||||||||||||
| RefSeq | NP_003055.1. | ||||||||||||
| UniGene | Hs.517070 | ||||||||||||
3D structure databases | |||||||||||||
| |||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | P03973. | ||||||||||||
Protein family/group databases | |||||||||||||
| MEROPS | I17.001. I17.950. | ||||||||||||
Proteomic databases | |||||||||||||
| PeptideAtlas | P03973. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000124107. Homo sapiens. [Contig view] | ||||||||||||
| GeneID | 6590. | ||||||||||||
| KEGG | hsa:6590. | ||||||||||||
Organism-specific databases | |||||||||||||
| H-InvDB | HIX0015854. | ||||||||||||
| HGNC | HGNC:11092. SLPI. | ||||||||||||
| HPA | CAB002303. | ||||||||||||
| MIM | 107285. gene. | ||||||||||||
| PharmGKB | PA35944. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
| GeneCards | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOVERGEN | P03973. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P03973. | ||||||||||||
| CleanEx | HS_SLPI. | ||||||||||||
| GermOnline | ENSG00000124107. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR015874. 4-disulphide_core. IPR008197. Whey_acidic_protein_4-diS_core. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:4.10.75.10. Whey_acidic_protein_4-diS_core. 2 hits. | ||||||||||||
| Pfam | PF00095. WAP. 2 hits. [Graphical view] | ||||||||||||
| PRINTS | PR00003. 4DISULPHCORE. | ||||||||||||
| ProDom | PD001224. Prot_inh_I17. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||
| SMART | SM00217. WAP. 2 hits. [Graphical view] | ||||||||||||
| PROSITE | PS51390. WAP. 2 hits. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 25637. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | SLPI_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P03973 Secondary accession number(s): P07757 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 20 Human chromosome 20: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


