Reviewed, UniProtKB/Swiss-Prot P03968 (FGF1_BOVIN)

Names and origin

Protein names

Recommended name:
    Heparin-binding growth factor 1
      Short name=HBGF-1
Alternative name(s):
    Acidic fibroblast growth factor
      Short name=aFGF
    Prostatropin
    Endothelial cell growth factor beta and alpha chains
    Acidic eye-derived growth factor II
      Short name=EDGF II
Cleaved into the following 2 chains:
    1- Recommended name:
            Endothelial cell growth factor beta
    2- Recommended name:
            Endothelial cell growth factor alpha

Gene names

Name:	FGF1
Synonyms:	AFGF, FGF-1, FGFA, HBGF-1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Subunit structure	Monomer. Binds FGFR2. Forms a ternary complex containing 2 molecules each of FGFR2 and FGF1 for 1 heparin molecule. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1 By similarity.
Miscellaneous	This protein binds heparin, although less strongly than does bFGF.
Sequence similarities	Belongs to the heparin-binding growth factors family.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation
Ligand	Heparin-binding
Molecular function	Developmental protein Growth factor Mitogen
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	angiogenesis Inferred from electronic annotation. Source: UniProtKB-KW cell proliferation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.4 Ref.5

Chain

2 – 155

154

Endothelial cell growth factor beta

PRO_0000008904

Chain

16 – 155

140

Heparin-binding growth factor 1

PRO_0000008905

Chain

22 – 155

134

Endothelial cell growth factor alpha

PRO_0000008906

Regions

Region

24 – 28

5

Heparin-binding Potential

Region

113 – 116

4

Heparin-binding Potential

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.4

Secondary structure

1

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155

Helix Strand Turn

Details...

Beta strand

27 – 31

5

Turn

32 – 34

3

Beta strand

37 – 40

4

Beta strand

46 – 50

5

Helix

55 – 57

3

Beta strand

59 – 61

3

Beta strand

65 – 67

3

Beta strand

71 – 76

6

Beta strand

79 – 82

4

Beta strand

86 – 93

8

Helix

96 – 98

3

Beta strand

113 – 115

3

Turn

116 – 121

6

Helix

135 – 137

3

Beta strand

147 – 150

4

Sequences

	Sequence		Length	Mass (Da)
	P03968-1 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: F636641F189F9BFD	FASTA	155	17,493
	10 20 30 40 50 60 MAEGETTTFT ALTEKFNLPL GNYKKPKLLY CSNGGYFLRI LPDGTVDGTK DRSDQHIQLQ 70 80 90 100 110 120 LCAESIGEVY IKSTETGQFL AMDTDGLLYG SQTPNEECLF LERLEENHYN TYISKKHAEK 130 140 150 HWFVGLKKNG RSKLGPRTHF GQKAILFLPL PVSSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of bovine acidic fibroblast growth factor cDNA." Halley C., Courtois Y., Laurent M. Nucleic Acids Res. 16:10913-10913(1988) [PubMed: 3205724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	"Characterization of a bovine acidic FGF cDNA clone and its expression in brain and retina." Alterio J., Halley C., Brou C., Soussi T., Courtois Y., Laurent M. FEBS Lett. 242:41-46(1988) [PubMed: 2849564] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.
[4]	"Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial cell growth factor alpha and acidic fibroblast growth factor." Burgess W.H., Mehlman T., Marshak D.R., Fraser B.A., Maciag T. Proc. Natl. Acad. Sci. U.S.A. 83:7216-7220(1986) [PubMed: 3532107] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-155, ACETYLATION AT ALA-2.
[5]	"Complete primary structure of prostatropin, a prostate epithelial cell growth factor." Crabb J.W., Armes L.G., Carr S.A., Johnson C.M., Roberts G.D., Bordoli R.S., McKeehan W.L. Biochemistry 25:4988-4993(1986) [PubMed: 3768327] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-155.
[6]	"Brain-derived acidic fibroblast growth factor: complete amino acid sequence and homologies." Gimenez-Gallego G., Rodkey J., Bennett C., Rios-Candelore M., Disalvo J., Thomas K. Science 230:1385-1388(1985) [PubMed: 4071057] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-155.
[7]	"Acidic fibroblast growth factor (FGF) from bovine brain: amino-terminal sequence and comparison with basic FGF." Boehlen P., Esch F., Baird A., Gospodarowicz D. EMBO J. 4:1951-1956(1985) [PubMed: 4065099] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-44, AMINO-ACID COMPOSITION.
[8]	"Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor." Abraham J.A., Mergia A., Whang J.L., Tumolo A., Friedman J., Hjerrild K.A., Gospodarowicz D., Fiddes J.C. Science 233:545-548(1986) [PubMed: 2425435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-56.
[9]	"Isolation of heparin-binding growth factors from bovine, porcine and canine hearts." Quinkler W., Maasberg M., Bernotat-Danielowski S., Luethe N., Sharma H.S., Schaper W. Eur. J. Biochem. 181:67-73(1989) [PubMed: 2714282] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-45.
[10]	"Cloning of two different 5' untranslated exons of bovine acidic fibroblast growth factor by the single strand ligation to single-stranded cDNA methodology." Philippe J.-M., Renaud F., Desset S., Laurent M., Mallet J., Courtois Y., Edwards J.B. Biochem. Biophys. Res. Commun. 188:843-850(1992) [PubMed: 1280126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-18.
[11]	"Three-dimensional structures of acidic and basic fibroblast growth factors." Zhu X., Komiya H., Chirino A., Faham S., Fox G.M., Arakawa T., Hsu B.T., Rees D.C. Science 251:90-93(1991) [PubMed: 1702556] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X13221 mRNA. Translation: CAA31610.1.
X14032 mRNA. Translation: CAA32192.1.
M35608 mRNA. Translation: AAA30517.1.
BC103225 mRNA. Translation: AAI03226.1.
M13439 Genomic DNA. Translation: AAA30516.1.
X66446 mRNA. Translation: CAA47063.1.
M97660 mRNA. Translation: AAA30563.1.
M97661 mRNA. Translation: AAA30564.1.

IPI

IPI00708726.

PIR

GKBOA. JH0613.

RefSeq

NP_776480.1.

UniGene

Bt.5038

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AFC	X-ray	2.70	A/B/C/D/E/F/G/H	16-155	[»]
1BAR	X-ray	2.70	A/B	16-155	[»]

SMR

P03968. Positions 5-155.

ModBase

Search...

Genome annotation databases

Ensembl

ENSBTAG00000005198. Bos taurus. [Contig view]

GeneID

281160.

KEGG

bta:281160.

Phylogenomic databases

HOVERGEN

P03968.

OMA

P03968. PSEECLF.

Family and domain databases

InterPro

IPR002209. GF_heparin_bd.
IPR002348. IL1_HBGF.
[Graphical view]

PANTHER

PTHR11486. IL1_HBGF. 1 hit.

Pfam

PF00167. FGF. 1 hit.
[Graphical view]

PRINTS

PR00263. HBGFFGF.
PR00262. IL1HBGF.

ProDom

PD000831. IL1_HBGF. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00442. FGF. 1 hit.
[Graphical view]

PROSITE

PS00247. HBGF_FGF. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FGF1_BOVIN

Accession

Primary (citable) accession number: P03968
Secondary accession number(s): Q3ZBL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)