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P03966

- MYCN_MOUSE

UniProt

P03966 - MYCN_MOUSE

Protein

N-myc proto-oncogene protein

Gene

Mycn

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: MGI
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. branching morphogenesis of an epithelial tube Source: MGI
    2. cartilage condensation Source: MGI
    3. embryonic digit morphogenesis Source: MGI
    4. embryonic skeletal system morphogenesis Source: MGI
    5. lung development Source: MGI
    6. negative regulation of astrocyte differentiation Source: MGI
    7. negative regulation of reactive oxygen species metabolic process Source: UniProtKB
    8. positive regulation of cell death Source: MGI
    9. positive regulation of cell proliferation Source: MGI
    10. positive regulation of mesenchymal cell proliferation Source: MGI
    11. positive regulation of transcription, DNA-templated Source: UniProtKB
    12. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-myc proto-oncogene protein
    Gene namesi
    Name:Mycn
    Synonyms:Nmyc, Nmyc1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:97357. Mycn.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 462462N-myc proto-oncogene proteinPRO_0000127325Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei259 – 2591Phosphoserine; by CK2By similarity
    Modified residuei261 – 2611Phosphoserine; by CK2By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP03966.

    PTM databases

    PhosphoSiteiP03966.

    Expressioni

    Developmental stagei

    Expressed in the proliferating cells of the developing CNS and the epidermis. In the spinal cord at embryonic days 10.5, 11.5 and 12.5 dpc, expressed in the proliferating cells of the ventricular zone of the neural tube and is expressed at reduced levels in the intermediate zone. At 14.5 dpc, found in regions containing differentiating post-mitotic neurons. In the developing epidermis at 17 dpc, expression is restricted to primary hair germ cells only.1 Publication

    Gene expression databases

    ArrayExpressiP03966.
    BgeeiP03966.
    GenevestigatoriP03966.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with KDM5A, KDM5B and HUWE1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201795. 5 interactions.
    DIPiDIP-49592N.
    IntActiP03966. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP03966.
    SMRiP03966. Positions 380-458.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini379 – 43153bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni431 – 45222Leucine-zipperAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi260 – 27617Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG83719.
    HOGENOMiHOG000043075.
    HOVERGENiHBG000472.
    InParanoidiP03966.
    KOiK09109.
    OMAiGMICKNP.
    OrthoDBiEOG7GJ6CX.
    PhylomeDBiP03966.
    TreeFamiTF106001.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR002418. Tscrpt_reg_Myc.
    IPR012682. Tscrpt_reg_Myc_N.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    PF01056. Myc_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001705. Myc_protein. 1 hit.
    PRINTSiPR00044. LEUZIPPRMYC.
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03966-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSCTASTMP GMICKNPDLE FDSLQPCFYP DEDDFYFGGP DSTPPGEDIW    50
    KKFELLPTPP LSPSRAFPEH SPEPSNWATE MLLPEADLWG NPAEEDAFGL 100
    GGLGGLTPNP VILQDCMWSG FSAREKLERA VNEKLQHGHG PPGVSSACSA 150
    PGVGASSPGG RALGGSSSAS HTGATLPTDL SHPAAECVDP AVVFPFPVNK 200
    RESASVPAAP TSAPATSAAV TSVSVPATAP VAAPARAGGR PASSGEAKAL 250
    STSGEDTLSD SDDEDDEEED EEEEIDVVTV EKRRSSSNNK AVTTFTITVR 300
    PKTSALGLGR AQPGELILKR CVPIHQQHNY AAPSPYVESE DAPPQKKIKS 350
    EASPRPLKSV VPAKAKSLSP RNSDSEDSER RRNHNILERQ RRNDLRSSFL 400
    TLRDHVPELV KNEKAAKVVI LKKATEYVHA LQANEHQLLL EKEKLQARQQ 450
    QLLKKIEHAR TC 462
    Length:462
    Mass (Da):49,572
    Last modified:December 15, 1998 - v2
    Checksum:iE7AFD3DCBCABF290
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 292FY → LH in AAA39788. (PubMed:3513190)Curated
    Sequence conflicti36 – 361Y → H in AAA39788. (PubMed:3513190)Curated
    Sequence conflicti246 – 2472EA → GT in AAA39788. (PubMed:3513190)Curated
    Sequence conflicti313 – 3131P → L in AAA39788. (PubMed:3513190)Curated
    Sequence conflicti390 – 3901Q → E in CAA27557. (PubMed:3015591)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12731 Genomic DNA. Translation: AAA39788.1.
    X03919 Genomic DNA. Translation: CAA27557.1.
    M36277 mRNA. Translation: AAA39833.1.
    M29208 Genomic DNA. Translation: AAA39830.1.
    X06993 Genomic DNA. No translation available.
    CCDSiCCDS25818.1.
    PIRiA01356. TVMSMC.
    A01357. TVMSM2.
    RefSeqiNP_032735.3. NM_008709.3.
    UniGeneiMm.16469.

    Genome annotation databases

    EnsembliENSMUST00000043396; ENSMUSP00000045993; ENSMUSG00000037169.
    ENSMUST00000130990; ENSMUSP00000114225; ENSMUSG00000037169.
    GeneIDi18109.
    KEGGimmu:18109.
    UCSCiuc007nbf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12731 Genomic DNA. Translation: AAA39788.1 .
    X03919 Genomic DNA. Translation: CAA27557.1 .
    M36277 mRNA. Translation: AAA39833.1 .
    M29208 Genomic DNA. Translation: AAA39830.1 .
    X06993 Genomic DNA. No translation available.
    CCDSi CCDS25818.1.
    PIRi A01356. TVMSMC.
    A01357. TVMSM2.
    RefSeqi NP_032735.3. NM_008709.3.
    UniGenei Mm.16469.

    3D structure databases

    ProteinModelPortali P03966.
    SMRi P03966. Positions 380-458.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201795. 5 interactions.
    DIPi DIP-49592N.
    IntActi P03966. 2 interactions.

    PTM databases

    PhosphoSitei P03966.

    Proteomic databases

    PRIDEi P03966.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043396 ; ENSMUSP00000045993 ; ENSMUSG00000037169 .
    ENSMUST00000130990 ; ENSMUSP00000114225 ; ENSMUSG00000037169 .
    GeneIDi 18109.
    KEGGi mmu:18109.
    UCSCi uc007nbf.1. mouse.

    Organism-specific databases

    CTDi 4613.
    MGIi MGI:97357. Mycn.

    Phylogenomic databases

    eggNOGi NOG83719.
    HOGENOMi HOG000043075.
    HOVERGENi HBG000472.
    InParanoidi P03966.
    KOi K09109.
    OMAi GMICKNP.
    OrthoDBi EOG7GJ6CX.
    PhylomeDBi P03966.
    TreeFami TF106001.

    Miscellaneous databases

    NextBioi 293301.
    PROi P03966.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P03966.
    Bgeei P03966.
    Genevestigatori P03966.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR002418. Tscrpt_reg_Myc.
    IPR012682. Tscrpt_reg_Myc_N.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    PF01056. Myc_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001705. Myc_protein. 1 hit.
    PRINTSi PR00044. LEUZIPPRMYC.
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Nucleotide sequence of the coding region of the mouse N-myc gene."
      Taya Y., Mizusawa S., Nishimura S.
      EMBO J. 5:1215-1219(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Expression of murine N-myc by insertion of retrovirus sequences in murine macrophage cell lines."
      Nasu N., Setoguchi M., Yoshiyama K., Matsuura K., Yamamoto S.
      Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE OF 1-456.
      Strain: BALB/c.
    4. "Insertional activation of N-myc by endogenous Moloney-like murine retrovirus sequences in macrophage cell lines derived from myeloma cell line-macrophage hybrids."
      Setoguchi M., Higuchi Y., Yoshida S., Nasu N., Miyazaki Y., Akizuki S., Yamamoto S.
      Mol. Cell. Biol. 9:4515-4522(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 327-462.
    5. "Complete nucleotide sequence and exon-intron boundaries of the 5' non-coding region of the mouse N-myc gene."
      Katoh K., Sawai S., Ueno K., Kondoh H.
      Nucleic Acids Res. 16:3589-3589(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
    6. "Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation."
      Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
      EMBO J. 14:5646-5659(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiMYCN_MOUSE
    AccessioniPrimary (citable) accession number: P03966
    Secondary accession number(s): Q61978
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 1986
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3