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Protein

N-myc proto-oncogene protein

Gene

Mycn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Positively regulates the transcription of MYCNOS in neuroblastoma cells.By similarity

GO - Molecular functioni

GO - Biological processi

  • branching morphogenesis of an epithelial tube Source: MGI
  • cartilage condensation Source: MGI
  • embryonic digit morphogenesis Source: MGI
  • embryonic skeletal system morphogenesis Source: MGI
  • lung development Source: MGI
  • negative regulation of astrocyte differentiation Source: MGI
  • negative regulation of gene expression Source: MGI
  • negative regulation of reactive oxygen species metabolic process Source: UniProtKB
  • positive regulation of cell death Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of mesenchymal cell proliferation Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • regulation of auditory receptor cell differentiation Source: MGI

Keywordsi

Molecular functionDNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
N-myc proto-oncogene protein
Gene namesi
Name:Mycn
Synonyms:Nmyc, Nmyc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:97357. Mycn.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001273251 – 462N-myc proto-oncogene proteinAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei259Phosphoserine; by CK2By similarity1
Modified residuei261Phosphoserine; by CK2By similarity1

Post-translational modificationi

Phosphorylated by GSK3-beta which may promote its degradation. Phosphorylated by AURKA.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP03966.
PRIDEiP03966.

PTM databases

iPTMnetiP03966.
PhosphoSitePlusiP03966.

Expressioni

Developmental stagei

Expressed in the proliferating cells of the developing CNS and the epidermis. In the spinal cord at embryonic days 10.5, 11.5 and 12.5 dpc, expressed in the proliferating cells of the ventricular zone of the neural tube and is expressed at reduced levels in the intermediate zone. At 14.5 dpc, found in regions containing differentiating post-mitotic neurons. In the developing epidermis at 17 dpc, expression is restricted to primary hair germ cells only.1 Publication

Gene expression databases

BgeeiENSMUSG00000037169.
ExpressionAtlasiP03966. baseline and differential.
GenevisibleiP03966. MM.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with KDM5A, KDM5B and HUWE1. Interacts with MYCNOS. Interacts with AURKA; interaction is phospho-independent and triggers AURKA activation; AURKA competes with FBXW7 for binding to unphosphorylated MYCN but not for binding to unphosphorylated MYCN. Interacts with FBXW7; FBXW7 competes with AURKA for binding to unphosphorylated MYCN but not for binding to phosphorylated MYCN.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201795. 5 interactors.
DIPiDIP-49592N.
IntActiP03966. 4 interactors.
STRINGi10090.ENSMUSP00000045993.

Structurei

3D structure databases

ProteinModelPortaliP03966.
SMRiP03966.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini379 – 431bHLHPROSITE-ProRule annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 47Interaction with AURKABy similarityAdd BLAST29
Regioni61 – 90Interaction with AURKA and FBXW7By similarityAdd BLAST30
Regioni431 – 452Leucine-zipperAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi260 – 276Asp/Glu-rich (acidic)Add BLAST17

Phylogenomic databases

eggNOGiENOG410IJ8V. Eukaryota.
ENOG41105VW. LUCA.
GeneTreeiENSGT00510000046414.
HOGENOMiHOG000043075.
HOVERGENiHBG000472.
InParanoidiP03966.
KOiK09109.
OMAiFAEHSSE.
OrthoDBiEOG091G0ZJQ.
PhylomeDBiP03966.
TreeFamiTF106001.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
IPR036638. HLH_DNA-bd_sf.
IPR002418. Tscrpt_reg_Myc.
IPR012682. Tscrpt_reg_Myc_N.
PfamiView protein in Pfam
PF00010. HLH. 1 hit.
PF01056. Myc_N. 1 hit.
PIRSFiPIRSF001705. Myc_protein. 1 hit.
PRINTSiPR00044. LEUZIPPRMYC.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequencei

Sequence statusi: Complete.

P03966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSCTASTMP GMICKNPDLE FDSLQPCFYP DEDDFYFGGP DSTPPGEDIW
60 70 80 90 100
KKFELLPTPP LSPSRAFPEH SPEPSNWATE MLLPEADLWG NPAEEDAFGL
110 120 130 140 150
GGLGGLTPNP VILQDCMWSG FSAREKLERA VNEKLQHGHG PPGVSSACSA
160 170 180 190 200
PGVGASSPGG RALGGSSSAS HTGATLPTDL SHPAAECVDP AVVFPFPVNK
210 220 230 240 250
RESASVPAAP TSAPATSAAV TSVSVPATAP VAAPARAGGR PASSGEAKAL
260 270 280 290 300
STSGEDTLSD SDDEDDEEED EEEEIDVVTV EKRRSSSNNK AVTTFTITVR
310 320 330 340 350
PKTSALGLGR AQPGELILKR CVPIHQQHNY AAPSPYVESE DAPPQKKIKS
360 370 380 390 400
EASPRPLKSV VPAKAKSLSP RNSDSEDSER RRNHNILERQ RRNDLRSSFL
410 420 430 440 450
TLRDHVPELV KNEKAAKVVI LKKATEYVHA LQANEHQLLL EKEKLQARQQ
460
QLLKKIEHAR TC
Length:462
Mass (Da):49,572
Last modified:December 15, 1998 - v2
Checksum:iE7AFD3DCBCABF290
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28 – 29FY → LH in AAA39788 (PubMed:3513190).Curated2
Sequence conflicti36Y → H in AAA39788 (PubMed:3513190).Curated1
Sequence conflicti246 – 247EA → GT in AAA39788 (PubMed:3513190).Curated2
Sequence conflicti313P → L in AAA39788 (PubMed:3513190).Curated1
Sequence conflicti390Q → E in CAA27557 (PubMed:3015591).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12731 Genomic DNA. Translation: AAA39788.1.
X03919 Genomic DNA. Translation: CAA27557.1.
M36277 mRNA. Translation: AAA39833.1.
M29208 Genomic DNA. Translation: AAA39830.1.
X06993 Genomic DNA. No translation available.
CCDSiCCDS25818.1.
PIRiA01356. TVMSMC.
A01357. TVMSM2.
RefSeqiNP_032735.3. NM_008709.3.
UniGeneiMm.16469.

Genome annotation databases

EnsembliENSMUST00000043396; ENSMUSP00000045993; ENSMUSG00000037169.
ENSMUST00000130990; ENSMUSP00000114225; ENSMUSG00000037169.
GeneIDi18109.
KEGGimmu:18109.
UCSCiuc007nbf.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiMYCN_MOUSE
AccessioniPrimary (citable) accession number: P03966
Secondary accession number(s): Q61978
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: December 15, 1998
Last modified: October 25, 2017
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot