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Protein

N-myc proto-oncogene protein

Gene

Mycn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

GO - Molecular functioni

GO - Biological processi

  • branching morphogenesis of an epithelial tube Source: MGI
  • cartilage condensation Source: MGI
  • embryonic digit morphogenesis Source: MGI
  • embryonic skeletal system morphogenesis Source: MGI
  • lung development Source: MGI
  • negative regulation of astrocyte differentiation Source: MGI
  • negative regulation of reactive oxygen species metabolic process Source: UniProtKB
  • positive regulation of cell death Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of mesenchymal cell proliferation Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
N-myc proto-oncogene protein
Gene namesi
Name:Mycn
Synonyms:Nmyc, Nmyc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:97357. Mycn.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462N-myc proto-oncogene proteinPRO_0000127325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei259 – 2591Phosphoserine; by CK2By similarity
Modified residuei261 – 2611Phosphoserine; by CK2By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP03966.

PTM databases

PhosphoSiteiP03966.

Expressioni

Developmental stagei

Expressed in the proliferating cells of the developing CNS and the epidermis. In the spinal cord at embryonic days 10.5, 11.5 and 12.5 dpc, expressed in the proliferating cells of the ventricular zone of the neural tube and is expressed at reduced levels in the intermediate zone. At 14.5 dpc, found in regions containing differentiating post-mitotic neurons. In the developing epidermis at 17 dpc, expression is restricted to primary hair germ cells only.1 Publication

Gene expression databases

BgeeiP03966.
ExpressionAtlasiP03966. baseline and differential.
GenevisibleiP03966. MM.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with KDM5A, KDM5B and HUWE1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi201795. 5 interactions.
DIPiDIP-49592N.
IntActiP03966. 2 interactions.
STRINGi10090.ENSMUSP00000045993.

Structurei

3D structure databases

ProteinModelPortaliP03966.
SMRiP03966. Positions 380-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini379 – 43153bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni431 – 45222Leucine-zipperAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi260 – 27617Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG83719.
GeneTreeiENSGT00510000046414.
HOGENOMiHOG000043075.
HOVERGENiHBG000472.
InParanoidiP03966.
KOiK09109.
OMAiPEADLWG.
OrthoDBiEOG7GJ6CX.
PhylomeDBiP03966.
TreeFamiTF106001.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR002418. Tscrpt_reg_Myc.
IPR012682. Tscrpt_reg_Myc_N.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF01056. Myc_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001705. Myc_protein. 1 hit.
PRINTSiPR00044. LEUZIPPRMYC.
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSCTASTMP GMICKNPDLE FDSLQPCFYP DEDDFYFGGP DSTPPGEDIW
60 70 80 90 100
KKFELLPTPP LSPSRAFPEH SPEPSNWATE MLLPEADLWG NPAEEDAFGL
110 120 130 140 150
GGLGGLTPNP VILQDCMWSG FSAREKLERA VNEKLQHGHG PPGVSSACSA
160 170 180 190 200
PGVGASSPGG RALGGSSSAS HTGATLPTDL SHPAAECVDP AVVFPFPVNK
210 220 230 240 250
RESASVPAAP TSAPATSAAV TSVSVPATAP VAAPARAGGR PASSGEAKAL
260 270 280 290 300
STSGEDTLSD SDDEDDEEED EEEEIDVVTV EKRRSSSNNK AVTTFTITVR
310 320 330 340 350
PKTSALGLGR AQPGELILKR CVPIHQQHNY AAPSPYVESE DAPPQKKIKS
360 370 380 390 400
EASPRPLKSV VPAKAKSLSP RNSDSEDSER RRNHNILERQ RRNDLRSSFL
410 420 430 440 450
TLRDHVPELV KNEKAAKVVI LKKATEYVHA LQANEHQLLL EKEKLQARQQ
460
QLLKKIEHAR TC
Length:462
Mass (Da):49,572
Last modified:December 15, 1998 - v2
Checksum:iE7AFD3DCBCABF290
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 292FY → LH in AAA39788 (PubMed:3513190).Curated
Sequence conflicti36 – 361Y → H in AAA39788 (PubMed:3513190).Curated
Sequence conflicti246 – 2472EA → GT in AAA39788 (PubMed:3513190).Curated
Sequence conflicti313 – 3131P → L in AAA39788 (PubMed:3513190).Curated
Sequence conflicti390 – 3901Q → E in CAA27557 (PubMed:3015591).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12731 Genomic DNA. Translation: AAA39788.1.
X03919 Genomic DNA. Translation: CAA27557.1.
M36277 mRNA. Translation: AAA39833.1.
M29208 Genomic DNA. Translation: AAA39830.1.
X06993 Genomic DNA. No translation available.
CCDSiCCDS25818.1.
PIRiA01356. TVMSMC.
A01357. TVMSM2.
RefSeqiNP_032735.3. NM_008709.3.
UniGeneiMm.16469.

Genome annotation databases

EnsembliENSMUST00000043396; ENSMUSP00000045993; ENSMUSG00000037169.
ENSMUST00000130990; ENSMUSP00000114225; ENSMUSG00000037169.
GeneIDi18109.
KEGGimmu:18109.
UCSCiuc007nbf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12731 Genomic DNA. Translation: AAA39788.1.
X03919 Genomic DNA. Translation: CAA27557.1.
M36277 mRNA. Translation: AAA39833.1.
M29208 Genomic DNA. Translation: AAA39830.1.
X06993 Genomic DNA. No translation available.
CCDSiCCDS25818.1.
PIRiA01356. TVMSMC.
A01357. TVMSM2.
RefSeqiNP_032735.3. NM_008709.3.
UniGeneiMm.16469.

3D structure databases

ProteinModelPortaliP03966.
SMRiP03966. Positions 380-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201795. 5 interactions.
DIPiDIP-49592N.
IntActiP03966. 2 interactions.
STRINGi10090.ENSMUSP00000045993.

PTM databases

PhosphoSiteiP03966.

Proteomic databases

PRIDEiP03966.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043396; ENSMUSP00000045993; ENSMUSG00000037169.
ENSMUST00000130990; ENSMUSP00000114225; ENSMUSG00000037169.
GeneIDi18109.
KEGGimmu:18109.
UCSCiuc007nbf.2. mouse.

Organism-specific databases

CTDi4613.
MGIiMGI:97357. Mycn.

Phylogenomic databases

eggNOGiNOG83719.
GeneTreeiENSGT00510000046414.
HOGENOMiHOG000043075.
HOVERGENiHBG000472.
InParanoidiP03966.
KOiK09109.
OMAiPEADLWG.
OrthoDBiEOG7GJ6CX.
PhylomeDBiP03966.
TreeFamiTF106001.

Miscellaneous databases

NextBioi293301.
PROiP03966.
SOURCEiSearch...

Gene expression databases

BgeeiP03966.
ExpressionAtlasiP03966. baseline and differential.
GenevisibleiP03966. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR002418. Tscrpt_reg_Myc.
IPR012682. Tscrpt_reg_Myc_N.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF01056. Myc_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001705. Myc_protein. 1 hit.
PRINTSiPR00044. LEUZIPPRMYC.
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the coding region of the mouse N-myc gene."
    Taya Y., Mizusawa S., Nishimura S.
    EMBO J. 5:1215-1219(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Expression of murine N-myc by insertion of retrovirus sequences in murine macrophage cell lines."
    Nasu N., Setoguchi M., Yoshiyama K., Matsuura K., Yamamoto S.
    Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE OF 1-456.
    Strain: BALB/c.
  4. "Insertional activation of N-myc by endogenous Moloney-like murine retrovirus sequences in macrophage cell lines derived from myeloma cell line-macrophage hybrids."
    Setoguchi M., Higuchi Y., Yoshida S., Nasu N., Miyazaki Y., Akizuki S., Yamamoto S.
    Mol. Cell. Biol. 9:4515-4522(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 327-462.
  5. "Complete nucleotide sequence and exon-intron boundaries of the 5' non-coding region of the mouse N-myc gene."
    Katoh K., Sawai S., Ueno K., Kondoh H.
    Nucleic Acids Res. 16:3589-3589(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
  6. "Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation."
    Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
    EMBO J. 14:5646-5659(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiMYCN_MOUSE
AccessioniPrimary (citable) accession number: P03966
Secondary accession number(s): Q61978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: December 15, 1998
Last modified: July 22, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.