Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P03963 (TRPE_BACSU)

Last modified November 3, 2009. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Anthranilate synthase component 1
    EC=4.1.3.27
Alternative name(s):
    Anthranilate synthase component I
Gene names
Name: trpE
Ordered Locus Names: BSU22680
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Hide | Top

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Catalytic activity

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.

Subunit structure

Tetramer of two components I and two components II By similarity.

Miscellaneous

Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.

Sequence similarities

Belongs to the anthranilate synthase component I family.

Sequence caution

The sequence described in Ref.1 differs from that shown. Reason: Frameshift at positions 343 and 402.

The sequence described in Ref.2 differs from that shown. Reason: Frameshift at positions 343 and 402.

Hide | Top

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Aromatic amino acid biosynthesis
Tryptophan biosynthesis
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtryptophan biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionanthranilate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 515515Anthranilate synthase component 1
PRO_0000154079

Experimental info

Sequence conflict801A → P Ref.1
Sequence conflict801A → P Ref.2
Sequence conflict1201A → S Ref.1
Sequence conflict1201A → S Ref.2

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P03963-1 [UniParc].

Last modified July 1, 1993. Version 2.
Checksum: 33B88D9D80BCD991

FASTA51558,116
        10         20         30         40         50         60 
MNFQSNISAF LEDSLSHHTI PIVETFTVDT LTPIQMIEKL DREITYLLES KDDTSTWSRY 

        70         80         90        100        110        120 
SFIGLNPFLT IKEEQGRFSA ADQDSKSLYT GNELKEVLNW MNTTYKIKTP ELGIPFVGGA 

       130        140        150        160        170        180 
VGYLSYDMIP LIEPSVPSHT KETDMEKCML FVCRTLIAYD HETKNVHFIQ YARLTGEETK 

       190        200        210        220        230        240 
NEKMDVFHQN HLELQNLIEK MMDQKNIKEL FLSADSYKTP SFETVSSNYE KSAFMADVEK 

       250        260        270        280        290        300 
IKSYIKAGDI FQGVLSQKFE VPIKADAFEL YRVLRIVNPS PYMYYMKLLD REIVGSSPER 

       310        320        330        340        350        360 
LIHVQDGHLE IHPIAGTRKR GADKAEDERL KVELMKDEKE KAEHYMLVDL ARNDIGRVAE 

       370        380        390        400        410        420 
YGSVSVPEFT KIVSFSHVMH IISVVTGRLK KGVHPVDALM SAFPAGTLTG APKIRAMQLL 

       430        440        450        460        470        480 
QELEPTPRET YGGCIAYIGF DGNIDSCITI RTMSVKNGVA SIQAGAGIVA DSVPEAEYEE 

       490        500        510 
SCNKAGALLK TIHIAEDMFH SKEDKADEQI STIVR

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the Bacillus subtilis tryptophan operon."
Henner D.J., Band L., Shimotsu H.
Gene 34:169-177(1985) [PubMed: 3924737] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence of the Bacillus subtilis trpE and trpD genes."
Band L., Shimotsu H., Henner D.J.
Gene 27:55-65(1984) [PubMed: 6425119] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence of Bacillus subtilis dbpA, mtr(A,B), gerC(1-3), ndk, cheR, aro(B,E,F,H), trp(A-F), hisH, and tyrA genes."
Henner D.J.
Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[5]"Characterization of the Bacillus subtilis tryptophan promoter region."
Shimotsu H., Henner D.J.
Proc. Natl. Acad. Sci. U.S.A. 81:6315-6319(1984) [PubMed: 6436812] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
[6]"Novel form of transcription attenuation regulates expression the Bacillus subtilis tryptophan operon."
Shimotsu H., Kuroda M.I., Yanofsky C., Henner D.J.
J. Bacteriol. 166:461-471(1986) [PubMed: 2422155] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.

Hide | Top

Cross-references

Sequence databases

K01391 Genomic DNA. Translation: AAA22865.1. Frameshift.
M80245 Genomic DNA. Translation: AAA20862.1.
AL009126 Genomic DNA. Translation: CAB14184.1.
M27566 Genomic DNA. Translation: AAA22875.1.
PIRNNBS1. A01119.
RefSeqNP_390149.1.

3D structure databases

HSSPHSSP built from PDB template 1K0G based on UniProtKB P05041.
ModBaseSearch...

Genome annotation databases

GeneID939008.
GenomeReviewsGene locus BSU22680 in contig AL009126_GR.
KEGGbsu:BSU22680.
NMPDRfig|224308.1.peg.2272.

Organism-specific databases

SubtiListBG10287. trpE. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP03963.
OMARIANPSP.

Enzyme and pathway databases

BioCycBSUB224308:BSU2267-MON.
BRENDA4.1.3.27. 150.

Family and domain databases

InterProIPR005801. ADC_synthase.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synthI.
IPR019999. Anthranilate_synth_I_C.
IPR015890. Chorismate-bd_C.
[Graphical view]
Gene3DG3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
PANTHERPTHR11236. TRPE_1_chor_bd. 1 hit.
PfamPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSPR00095. ANTSNTHASEI.
ProDomPD000779. Anth_synth_chor. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00564. trpE_most. 1 hit.
ProtoNetSearch...

Hide | Top

Entry information

Entry nameTRPE_BACSU
AccessionPrimary (citable) accession number: P03963
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: July 1, 1993
Last modified: November 3, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents