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Protein

Potassium-transporting ATPase KdpC subunit

Gene

kdpC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm (PubMed:2849541, PubMed:8499455, PubMed:23930894). This subunit acts as a catalytic chaperone that increases the ATP-binding affinity of the ATP-hydrolyzing subunit KdpB by the formation of a transient KdpB/KdpC/ATP ternary complex (PubMed:21711450).4 Publications

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • potassium-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  • potassium ion transmembrane transport Source: GOC
  • potassium ion transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciEcoCyc:EG10515-MONOMER.
ECOL316407:JW0684-MONOMER.
MetaCyc:EG10515-MONOMER.

Protein family/group databases

TCDBi3.A.3.7.1. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase KdpC subunitUniRule annotation
Alternative name(s):
ATP phosphohydrolase [potassium-transporting] C chainUniRule annotation
Potassium-binding and translocating subunit CUniRule annotation
Potassium-translocating ATPase C chainUniRule annotation
Gene namesi
Name:kdpCUniRule annotation
Ordered Locus Names:b0696, JW0684
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10515. kdpC.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99Periplasmic1 Publication
Transmembranei10 – 3021HelicalUniRule annotationAdd
BLAST
Topological domaini31 – 190160Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • integral component of plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi140 – 15011QIPRVAKARNL → AIPAASKSANA: Cell does not grow at low potassium concentrations. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 190190Potassium-transporting ATPase KdpC subunitPRO_0000196990Add
BLAST

Proteomic databases

PaxDbiP03961.

Expressioni

Inductioni

Transcriptionally regulated by the KdpD/KdpE two-component regulatory system.1 Publication

Interactioni

Subunit structurei

The system is composed of three essential subunits: KdpA, KdpB and KdpC (PubMed:2849541, PubMed:9858692). The complex also contains KdpF, a small non-essential subunit (PubMed:10608856). The KdpFABC complex exists as a dimer above concentrations of 30-50 nM, whereas the complex exists as a functional monomer at lower concentrations (PubMed:18298081).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
kdpBP039602EBI-6997216,EBI-1116956

Protein-protein interaction databases

BioGridi4259349. 15 interactions.
IntActiP03961. 1 interaction.
MINTiMINT-8188885.
STRINGi511145.b0696.

Structurei

3D structure databases

ProteinModelPortaliP03961.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KdpC family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108R80. Bacteria.
COG2156. LUCA.
HOGENOMiHOG000244124.
InParanoidiP03961.
KOiK01548.
OMAiTVPAPYN.
OrthoDBiEOG6J751B.
PhylomeDBiP03961.

Family and domain databases

HAMAPiMF_00276. KdpC.
InterProiIPR003820. KdpC.
[Graphical view]
PANTHERiPTHR30042:SF2. PTHR30042:SF2. 1 hit.
PfamiPF02669. KdpC. 1 hit.
[Graphical view]
PIRSFiPIRSF001296. K_ATPase_KdpC. 1 hit.
TIGRFAMsiTIGR00681. kdpC. 1 hit.

Sequencei

Sequence statusi: Complete.

P03961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLRPALST FIFLLLITGG VYPLLTTVLG QWWFPWQANG SLIREGDTVR
60 70 80 90 100
GSALIGQNFT GNGYFHGRPS ATAEMPYNPQ ASGGSNLAVS NPELDKLIAA
110 120 130 140 150
RVAALRAANP DASASVPVEL VTASASGLDN NITPQAAAWQ IPRVAKARNL
160 170 180 190
SVEQLTQLIA KYSQQPLVKY IGQPVVNIVE LNLALDKLDE
Length:190
Mass (Da):20,267
Last modified:October 23, 1986 - v1
Checksum:i17F58EC839B6B95A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02670 Genomic DNA. Translation: AAB96337.1.
U00096 Genomic DNA. Translation: AAC73790.1.
AP009048 Genomic DNA. Translation: BAA35353.1.
PIRiA01073. PWECCK.
RefSeqiNP_415224.1. NC_000913.3.
WP_001300431.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73790; AAC73790; b0696.
BAA35353; BAA35353; BAA35353.
GeneIDi947508.
KEGGiecj:JW0684.
eco:b0696.
PATRICi32116587. VBIEscCol129921_0726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02670 Genomic DNA. Translation: AAB96337.1.
U00096 Genomic DNA. Translation: AAC73790.1.
AP009048 Genomic DNA. Translation: BAA35353.1.
PIRiA01073. PWECCK.
RefSeqiNP_415224.1. NC_000913.3.
WP_001300431.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP03961.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259349. 15 interactions.
IntActiP03961. 1 interaction.
MINTiMINT-8188885.
STRINGi511145.b0696.

Protein family/group databases

TCDBi3.A.3.7.1. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbiP03961.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73790; AAC73790; b0696.
BAA35353; BAA35353; BAA35353.
GeneIDi947508.
KEGGiecj:JW0684.
eco:b0696.
PATRICi32116587. VBIEscCol129921_0726.

Organism-specific databases

EchoBASEiEB0510.
EcoGeneiEG10515. kdpC.

Phylogenomic databases

eggNOGiENOG4108R80. Bacteria.
COG2156. LUCA.
HOGENOMiHOG000244124.
InParanoidiP03961.
KOiK01548.
OMAiTVPAPYN.
OrthoDBiEOG6J751B.
PhylomeDBiP03961.

Enzyme and pathway databases

BioCyciEcoCyc:EG10515-MONOMER.
ECOL316407:JW0684-MONOMER.
MetaCyc:EG10515-MONOMER.

Miscellaneous databases

PROiP03961.

Family and domain databases

HAMAPiMF_00276. KdpC.
InterProiIPR003820. KdpC.
[Graphical view]
PANTHERiPTHR30042:SF2. PTHR30042:SF2. 1 hit.
PfamiPF02669. KdpC. 1 hit.
[Graphical view]
PIRSFiPIRSF001296. K_ATPase_KdpC. 1 hit.
TIGRFAMsiTIGR00681. kdpC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence homology between two membrane transport ATPases, the Kdp-ATPase of Escherichia coli and the Ca2+-ATPase of sarcoplasmic reticulum."
    Hesse J.E., Wieczorek L., Altendorf K., Reicin A.S., Dorus E., Epstein W.
    Proc. Natl. Acad. Sci. U.S.A. 81:4746-4750(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators."
    Walderhaug M.O., Polarek J.W., Voelkner P., Daniel J.M., Hesse J.E., Altendorf K., Epstein W.
    J. Bacteriol. 174:2152-2159(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 143-190.
  6. "The K+-translocating Kdp-ATPase from Escherichia coli. Purification, enzymatic properties and production of complex- and subunit-specific antisera."
    Siebers A., Altendorf K.
    Eur. J. Biochem. 178:131-140(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN POTASSIUM TRANSPORT, SUBUNIT, SUBCELLULAR LOCATION.
  7. "The products of the kdpDE operon are required for expression of the Kdp ATPase of Escherichia coli."
    Polarek J.W., Williams G., Epstein W.
    J. Bacteriol. 174:2145-2151(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12.
  8. "ATP-driven potassium transport in right-side-out membrane vesicles via the Kdp system of Escherichia coli."
    Kollmann R., Altendorf K.
    Biochim. Biophys. Acta 1143:62-66(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN POTASSIUM TRANSPORT.
    Strain: K12.
  9. "Assembly of the Kdp complex, the multi-subunit K+-transport ATPase of Escherichia coli."
    Gassel M., Siebers A., Epstein W., Altendorf K.
    Biochim. Biophys. Acta 1415:77-84(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
    Strain: K12.
  10. "The KdpF subunit is part of the K(+)-translocating Kdp complex of Escherichia coli and is responsible for stabilization of the complex in vitro."
    Gassel M., Mollenkamp T., Puppe W., Altendorf K.
    J. Biol. Chem. 274:37901-37907(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
    Strain: K12.
  11. "K+-translocating KdpFABC P-type ATPase from Escherichia coli acts as a functional and structural dimer."
    Heitkamp T., Kalinowski R., Boettcher B., Boersch M., Altendorf K., Greie J.C.
    Biochemistry 47:3564-3575(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  12. "The KdpC subunit of the Escherichia coli K+-transporting KdpB P-type ATPase acts as a catalytic chaperone."
    Irzik K., Pfroetzschner J., Goss T., Ahnert F., Haupt M., Greie J.C.
    FEBS J. 278:3041-3053(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ATP-BINDING, MUTAGENESIS OF 140-GLN--LEU-150.
  13. "Mechanistic analysis of the pump cycle of the KdpFABC P-type ATPase."
    Damnjanovic B., Weber A., Potschies M., Greie J.C., Apell H.J.
    Biochemistry 52:5563-5576(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiKDPC_ECOLI
AccessioniPrimary (citable) accession number: P03961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: October 23, 1986
Last modified: January 20, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.