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Protein

Potassium-transporting ATPase potassium-binding subunit

Gene

kdpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm (PubMed:2849541, PubMed:8499455, PubMed:23930894). This subunit binds and transports the potassium across the cytoplasmic membrane (PubMed:7896809).4 Publications

GO - Molecular functioni

  • potassium ion binding Source: EcoCyc
  • potassium-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  • potassium ion transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciEcoCyc:EG10513-MONOMER.
ECOL316407:JW0686-MONOMER.
MetaCyc:EG10513-MONOMER.
BRENDAi3.6.3.12. 2026.

Protein family/group databases

TCDBi3.A.3.7.1. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase potassium-binding subunitUniRule annotation
Alternative name(s):
ATP phosphohydrolase [potassium-transporting] A chainUniRule annotation
Potassium-binding and translocating subunit AUniRule annotation
Potassium-translocating ATPase A chainUniRule annotation
Gene namesi
Name:kdpAUniRule annotation
Ordered Locus Names:b0698, JW0686
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10513. kdpA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44Periplasmic1 Publication
Transmembranei5 – 2521HelicalUniRule annotationAdd
BLAST
Topological domaini26 – 6237Cytoplasmic1 PublicationAdd
BLAST
Transmembranei63 – 8321HelicalUniRule annotationAdd
BLAST
Topological domaini84 – 13148Periplasmic1 PublicationAdd
BLAST
Transmembranei132 – 15221HelicalUniRule annotationAdd
BLAST
Topological domaini153 – 16917Cytoplasmic1 PublicationAdd
BLAST
Transmembranei170 – 19021HelicalUniRule annotationAdd
BLAST
Topological domaini191 – 25262Periplasmic1 PublicationAdd
BLAST
Transmembranei253 – 27321HelicalUniRule annotationAdd
BLAST
Topological domaini274 – 2829Cytoplasmic1 Publication
Transmembranei283 – 30321HelicalUniRule annotationAdd
BLAST
Topological domaini304 – 32825Periplasmic1 PublicationAdd
BLAST
Transmembranei329 – 34921HelicalUniRule annotationAdd
BLAST
Topological domaini350 – 41566Cytoplasmic1 PublicationAdd
BLAST
Transmembranei416 – 43621HelicalUniRule annotationAdd
BLAST
Topological domaini437 – 48347Periplasmic1 PublicationAdd
BLAST
Transmembranei484 – 50421HelicalUniRule annotationAdd
BLAST
Topological domaini505 – 52521Cytoplasmic1 PublicationAdd
BLAST
Transmembranei526 – 54621HelicalUniRule annotationAdd
BLAST
Topological domaini547 – 55711Periplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB-HAMAP
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi232 – 2321G → A or S: Decrease in K(+) affinity and loss of cation selectivity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Potassium-transporting ATPase potassium-binding subunitPRO_0000166494Add
BLAST

Proteomic databases

EPDiP03959.
PaxDbiP03959.

Expressioni

Inductioni

Transcriptionally regulated by the KdpD/KdpE two-component regulatory system.1 Publication

Interactioni

Subunit structurei

The system is composed of three essential subunits: KdpA, KdpB and KdpC (PubMed:2849541, PubMed:9858692). The complex also contains KdpF, a small non-essential subunit (PubMed:10608856). The KdpFABC complex exists as a dimer above concentrations of 30-50 nM, whereas the complex exists as a functional monomer at lower concentrations (PubMed:18298081).4 Publications

Protein-protein interaction databases

BioGridi4259932. 19 interactions.
STRINGi511145.b0698.

Structurei

3D structure databases

ProteinModelPortaliP03959.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KdpA family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D9K. Bacteria.
COG2060. LUCA.
HOGENOMiHOG000244102.
InParanoidiP03959.
KOiK01546.
OMAiNWQNYGG.
PhylomeDBiP03959.

Family and domain databases

HAMAPiMF_00275. KdpA. 1 hit.
InterProiIPR004623. KdpA.
[Graphical view]
PANTHERiPTHR30607. PTHR30607. 1 hit.
PfamiPF03814. KdpA. 1 hit.
[Graphical view]
PIRSFiPIRSF001294. K_ATPaseA. 1 hit.
TIGRFAMsiTIGR00680. kdpA. 1 hit.

Sequencei

Sequence statusi: Complete.

P03959-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQGFLLIA TFLLVLMVLA RPLGSGLARL INDIPLPGTT GVERVLFRAL
60 70 80 90 100
GVSDREMNWK QYLCAILGLN MLGLAVLFFM LLGQHYLPLN PQQLPGLSWD
110 120 130 140 150
LALNTAVSFV TNTNWQSYSG ETTLSYFSQM AGLTVQNFLS AASGIAVIFA
160 170 180 190 200
LIRAFTRQSM STLGNAWVDL LRITLWVLVP VALLIALFFI QQGALQNFLP
210 220 230 240 250
YQAVNTVEGA QQLLPMGPVA SQEAIKMLGT NGGGFFNANS SHPFENPTAL
260 270 280 290 300
TNFVQMLAIF LIPTALCFAF GEVMGDRRQG RMLLWAMSVI FVICVGVVMW
310 320 330 340 350
AEVQGNPHLL ALGTDSSINM EGKESRFGVL VSSLFAVVTT AASCGAVIAM
360 370 380 390 400
HDSFTALGGM VPMWLMQIGE VVFGGVGSGL YGMMLFVLLA VFIAGLMIGR
410 420 430 440 450
TPEYLGKKID VREMKLTALA ILVTPTLVLM GAALAMMTDA GRSAMLNPGP
460 470 480 490 500
HGFSEVLYAV SSAANNNGSA FAGLSANSPF WNCLLAFCMF VGRFGVIIPV
510 520 530 540 550
MAIAGSLVSK KSQAASSGTL PTHGPLFVGL LIGTVLLVGA LTFIPALALG

PVAEYLS
Length:557
Mass (Da):59,189
Last modified:August 13, 1987 - v1
Checksum:i88C397FC3D3DC17E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02670 Genomic DNA. Translation: AAB96335.1.
U00096 Genomic DNA. Translation: AAC73792.1.
AP009048 Genomic DNA. Translation: BAA35356.1.
PIRiA01071. PWECAK.
RefSeqiNP_415226.1. NC_000913.3.
WP_000741129.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73792; AAC73792; b0698.
BAA35356; BAA35356; BAA35356.
GeneIDi946045.
KEGGiecj:JW0686.
eco:b0698.
PATRICi32116591. VBIEscCol129921_0728.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02670 Genomic DNA. Translation: AAB96335.1.
U00096 Genomic DNA. Translation: AAC73792.1.
AP009048 Genomic DNA. Translation: BAA35356.1.
PIRiA01071. PWECAK.
RefSeqiNP_415226.1. NC_000913.3.
WP_000741129.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP03959.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259932. 19 interactions.
STRINGi511145.b0698.

Protein family/group databases

TCDBi3.A.3.7.1. the p-type atpase (p-atpase) superfamily.

Proteomic databases

EPDiP03959.
PaxDbiP03959.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73792; AAC73792; b0698.
BAA35356; BAA35356; BAA35356.
GeneIDi946045.
KEGGiecj:JW0686.
eco:b0698.
PATRICi32116591. VBIEscCol129921_0728.

Organism-specific databases

EchoBASEiEB0508.
EcoGeneiEG10513. kdpA.

Phylogenomic databases

eggNOGiENOG4105D9K. Bacteria.
COG2060. LUCA.
HOGENOMiHOG000244102.
InParanoidiP03959.
KOiK01546.
OMAiNWQNYGG.
PhylomeDBiP03959.

Enzyme and pathway databases

BioCyciEcoCyc:EG10513-MONOMER.
ECOL316407:JW0686-MONOMER.
MetaCyc:EG10513-MONOMER.
BRENDAi3.6.3.12. 2026.

Miscellaneous databases

PROiP03959.

Family and domain databases

HAMAPiMF_00275. KdpA. 1 hit.
InterProiIPR004623. KdpA.
[Graphical view]
PANTHERiPTHR30607. PTHR30607. 1 hit.
PfamiPF03814. KdpA. 1 hit.
[Graphical view]
PIRSFiPIRSF001294. K_ATPaseA. 1 hit.
TIGRFAMsiTIGR00680. kdpA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKDPA_ECOLI
AccessioniPrimary (citable) accession number: P03959
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: August 13, 1987
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Has two separate and distinct potassium-binding sites. One site is formed by three periplasmic loops and is inferred to be the site of initial binding. The other site is cytoplasmic.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.