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P03953 (CFAD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Complement factor D

EC=3.4.21.46
Alternative name(s):
28 kDa adipocyte protein
Adipsin
C3 convertase activator
Properdin factor D
Gene names
Name:Cfd
Synonyms:Adn, Df
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Catalytic activity

Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.

Subcellular location

Secreted.

Post-translational modification

N-glycosylated.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Biological processComplement alternate pathway
Immunity
Innate immunity
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcomplement activation, alternative pathway

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from direct assay PubMed 7592907. Source: MGI

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P03953-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P03953-2)

The sequence of this isoform differs from the canonical sequence as follows:
     20-20: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Propeptide21 – 255Activation peptide Potential
PRO_0000027562
Chain26 – 259234Complement factor D
PRO_0000027563

Regions

Domain26 – 254229Peptidase S1

Sites

Active site661Charge relay system
Active site1151Charge relay system
Active site2091Charge relay system

Amino acid modifications

Glycosylation461N-linked (GlcNAc...) Potential
Glycosylation1241N-linked (GlcNAc...) Ref.4
Glycosylation1761N-linked (GlcNAc...) Ref.5
Glycosylation2511N-linked (GlcNAc...) Potential
Glycosylation2561N-linked (GlcNAc...) Potential
Disulfide bond51 ↔ 67 By similarity
Disulfide bond149 ↔ 215 By similarity
Disulfide bond180 ↔ 196 By similarity
Disulfide bond205 ↔ 230 By similarity

Natural variations

Alternative sequence201Missing in isoform 2.
VSP_005382

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 23, 1986. Version 1.
Checksum: 8C3A952561247DF9

FASTA25928,057
        10         20         30         40         50         60 
MHSSVYFVAL VILGAAVCAA QPRGRILGGQ EAAAHARPYM ASVQVNGTHV CGGTLLDEQW 

        70         80         90        100        110        120 
VLSAAHCMDG VTDDDSVQVL LGAHSLSAPE PYKRWYDVQS VVPHPGSRPD SLEDDLILFK 

       130        140        150        160        170        180 
LSQNASLGPH VRPLPLQYED KEVEPGTLCD VAGWGVVTHA GRRPDVLHQL RVSIMNRTTC 

       190        200        210        220        230        240 
NLRTYHDGVV TINMMCAESN RRDTCRGDSG SPLVCGDAVE GVVTWGSRVC GNGKKPGVYT 

       250 
RVSSYRMWIE NITNGNMTS 

« Hide

Isoform 2 [UniParc].

Checksum: 85E1F17A26ECF7EC
Show »

FASTA25827,986

References

« Hide 'large scale' references
[1]"The nucleotide sequence of three genes participating in the adipose differentiation of 3T3 cells."
Phillips M., Djian P., Green H.
J. Biol. Chem. 261:10821-10827(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Adipsin, the adipocyte serine protease: gene structure and control of expression by tumor necrosis factor."
Min H.Y., Spiegelman B.M.
Nucleic Acids Res. 14:8879-8892(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
[3]"A developmentally regulated mRNA from 3T3 adipocytes encodes a novel serine protease homologue."
Cook K.S., Groves D.L., Min H.Y., Spiegelman B.M.
Proc. Natl. Acad. Sci. U.S.A. 82:6480-6484(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss white.
[4]"Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-124.
Strain: C57BL/6.
Tissue: Plasma.
[5]"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
Bernhard O.K., Kapp E.A., Simpson R.J.
J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-176.
Strain: C57BL/6.
Tissue: Plasma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11768 mRNA. Translation: AAA40486.1.
X04673 Genomic DNA. Translation: CAA28378.1.
X04673 Genomic DNA. Translation: CAA28379.1.
M13386 Genomic DNA. Translation: AAA37262.1.
PIRWMMS28. C25952.
RefSeqNP_038487.1. NM_013459.2.
XP_006513154.1. XM_006513091.1.
UniGeneMm.4407.

3D structure databases

ProteinModelPortalP03953.
SMRP03953. Positions 26-252.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP03953. 2 interactions.
MINTMINT-4090656.
STRING10090.ENSMUSP00000056836.

Protein family/group databases

MEROPSS01.191.

PTM databases

PhosphoSiteP03953.

Proteomic databases

PaxDbP03953.
PRIDEP03953.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000061653; ENSMUSP00000056836; ENSMUSG00000061780. [P03953-1]
GeneID11537.
KEGGmmu:11537.
UCSCuc007gaj.2. mouse. [P03953-1]

Organism-specific databases

CTD1675.
MGIMGI:87931. Cfd.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP03953.
KOK01334.
OMANMMCAES.
OrthoDBEOG7MKW6Q.
PhylomeDBP03953.
TreeFamTF333630.

Gene expression databases

ArrayExpressP03953.
BgeeP03953.
CleanExMM_CFD.
GenevestigatorP03953.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCFD. mouse.
NextBio278986.
PROP03953.
SOURCESearch...

Entry information

Entry nameCFAD_MOUSE
AccessionPrimary (citable) accession number: P03953
Secondary accession number(s): Q61280
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: October 23, 1986
Last modified: April 16, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot