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P03953

- CFAD_MOUSE

UniProt

P03953 - CFAD_MOUSE

Protein

Complement factor D

Gene

Cfd

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 1 (23 Oct 1986)
      Previous versions | rss
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    Functioni

    Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

    Catalytic activityi

    Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei66 – 661Charge relay system
    Active sitei115 – 1151Charge relay system
    Active sitei209 – 2091Charge relay system

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. complement activation, alternative pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Complement alternate pathway, Immunity, Innate immunity

    Enzyme and pathway databases

    ReactomeiREACT_218037. Alternative complement activation.

    Protein family/group databases

    MEROPSiS01.191.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Complement factor D (EC:3.4.21.46)
    Alternative name(s):
    28 kDa adipocyte protein
    Adipsin
    C3 convertase activator
    Properdin factor D
    Gene namesi
    Name:Cfd
    Synonyms:Adn, Df
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:87931. Cfd.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Propeptidei21 – 255Activation peptideSequence AnalysisPRO_0000027562
    Chaini26 – 259234Complement factor DPRO_0000027563Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi51 ↔ 67PROSITE-ProRule annotation
    Glycosylationi124 – 1241N-linked (GlcNAc...)1 Publication
    Disulfide bondi149 ↔ 215PROSITE-ProRule annotation
    Glycosylationi176 – 1761N-linked (GlcNAc...)1 Publication
    Disulfide bondi180 ↔ 196PROSITE-ProRule annotation
    Disulfide bondi205 ↔ 230PROSITE-ProRule annotation
    Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.2 Publications

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP03953.
    PaxDbiP03953.
    PRIDEiP03953.

    PTM databases

    PhosphoSiteiP03953.

    Expressioni

    Gene expression databases

    ArrayExpressiP03953.
    BgeeiP03953.
    CleanExiMM_CFD.
    GenevestigatoriP03953.

    Interactioni

    Protein-protein interaction databases

    IntActiP03953. 2 interactions.
    MINTiMINT-4090656.
    STRINGi10090.ENSMUSP00000056836.

    Structurei

    3D structure databases

    ProteinModelPortaliP03953.
    SMRiP03953. Positions 26-252.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 254229Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP03953.
    KOiK01334.
    OMAiNMMCAES.
    OrthoDBiEOG7MKW6Q.
    PhylomeDBiP03953.
    TreeFamiTF333630.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P03953-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHSSVYFVAL VILGAAVCAA QPRGRILGGQ EAAAHARPYM ASVQVNGTHV    50
    CGGTLLDEQW VLSAAHCMDG VTDDDSVQVL LGAHSLSAPE PYKRWYDVQS 100
    VVPHPGSRPD SLEDDLILFK LSQNASLGPH VRPLPLQYED KEVEPGTLCD 150
    VAGWGVVTHA GRRPDVLHQL RVSIMNRTTC NLRTYHDGVV TINMMCAESN 200
    RRDTCRGDSG SPLVCGDAVE GVVTWGSRVC GNGKKPGVYT RVSSYRMWIE 250
    NITNGNMTS 259
    Length:259
    Mass (Da):28,057
    Last modified:October 23, 1986 - v1
    Checksum:i8C3A952561247DF9
    GO
    Isoform 2 (identifier: P03953-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         20-20: Missing.

    Show »
    Length:258
    Mass (Da):27,986
    Checksum:i85E1F17A26ECF7EC
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei20 – 201Missing in isoform 2. CuratedVSP_005382

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11768 mRNA. Translation: AAA40486.1.
    X04673 Genomic DNA. Translation: CAA28378.1.
    X04673 Genomic DNA. Translation: CAA28379.1.
    M13386 Genomic DNA. Translation: AAA37262.1.
    CCDSiCCDS23995.1. [P03953-1]
    PIRiC25952. WMMS28.
    RefSeqiNP_001278844.1. NM_001291915.1. [P03953-2]
    NP_038487.1. NM_013459.3. [P03953-1]
    UniGeneiMm.4407.

    Genome annotation databases

    EnsembliENSMUST00000061653; ENSMUSP00000056836; ENSMUSG00000061780. [P03953-1]
    GeneIDi11537.
    KEGGimmu:11537.
    UCSCiuc007gaj.2. mouse. [P03953-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11768 mRNA. Translation: AAA40486.1 .
    X04673 Genomic DNA. Translation: CAA28378.1 .
    X04673 Genomic DNA. Translation: CAA28379.1 .
    M13386 Genomic DNA. Translation: AAA37262.1 .
    CCDSi CCDS23995.1. [P03953-1 ]
    PIRi C25952. WMMS28.
    RefSeqi NP_001278844.1. NM_001291915.1. [P03953-2 ]
    NP_038487.1. NM_013459.3. [P03953-1 ]
    UniGenei Mm.4407.

    3D structure databases

    ProteinModelPortali P03953.
    SMRi P03953. Positions 26-252.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P03953. 2 interactions.
    MINTi MINT-4090656.
    STRINGi 10090.ENSMUSP00000056836.

    Protein family/group databases

    MEROPSi S01.191.

    PTM databases

    PhosphoSitei P03953.

    Proteomic databases

    MaxQBi P03953.
    PaxDbi P03953.
    PRIDEi P03953.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000061653 ; ENSMUSP00000056836 ; ENSMUSG00000061780 . [P03953-1 ]
    GeneIDi 11537.
    KEGGi mmu:11537.
    UCSCi uc007gaj.2. mouse. [P03953-1 ]

    Organism-specific databases

    CTDi 1675.
    MGIi MGI:87931. Cfd.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P03953.
    KOi K01334.
    OMAi NMMCAES.
    OrthoDBi EOG7MKW6Q.
    PhylomeDBi P03953.
    TreeFami TF333630.

    Enzyme and pathway databases

    Reactomei REACT_218037. Alternative complement activation.

    Miscellaneous databases

    ChiTaRSi CFD. mouse.
    NextBioi 278986.
    PROi P03953.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P03953.
    Bgeei P03953.
    CleanExi MM_CFD.
    Genevestigatori P03953.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of three genes participating in the adipose differentiation of 3T3 cells."
      Phillips M., Djian P., Green H.
      J. Biol. Chem. 261:10821-10827(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Adipsin, the adipocyte serine protease: gene structure and control of expression by tumor necrosis factor."
      Min H.Y., Spiegelman B.M.
      Nucleic Acids Res. 14:8879-8892(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
    3. "A developmentally regulated mRNA from 3T3 adipocytes encodes a novel serine protease homologue."
      Cook K.S., Groves D.L., Min H.Y., Spiegelman B.M.
      Proc. Natl. Acad. Sci. U.S.A. 82:6480-6484(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss white.
    4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
      Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
      J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-124.
      Strain: C57BL/6.
      Tissue: Plasma.
    5. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
      Bernhard O.K., Kapp E.A., Simpson R.J.
      J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-176.
      Strain: C57BL/6.
      Tissue: Plasma.

    Entry informationi

    Entry nameiCFAD_MOUSE
    AccessioniPrimary (citable) accession number: P03953
    Secondary accession number(s): Q61280
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 1986
    Last sequence update: October 23, 1986
    Last modified: October 1, 2014
    This is version 140 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3