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Protein

Complement factor D

Gene

Cfd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Catalytic activityi

Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Charge relay system
Active sitei115 – 1151Charge relay system
Active sitei209 – 2091Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. complement activation, alternative pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement alternate pathway, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_218037. Alternative complement activation.

Protein family/group databases

MEROPSiS01.191.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement factor D (EC:3.4.21.46)
Alternative name(s):
28 kDa adipocyte protein
Adipsin
C3 convertase activator
Properdin factor D
Gene namesi
Name:Cfd
Synonyms:Adn, Df
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:87931. Cfd.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
  2. extracellular vesicular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Propeptidei21 – 255Activation peptideSequence AnalysisPRO_0000027562
Chaini26 – 259234Complement factor DPRO_0000027563Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi51 ↔ 67PROSITE-ProRule annotation
Glycosylationi124 – 1241N-linked (GlcNAc...)1 Publication
Disulfide bondi149 ↔ 215PROSITE-ProRule annotation
Glycosylationi176 – 1761N-linked (GlcNAc...)1 Publication
Disulfide bondi180 ↔ 196PROSITE-ProRule annotation
Disulfide bondi205 ↔ 230PROSITE-ProRule annotation
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP03953.
PaxDbiP03953.
PRIDEiP03953.

PTM databases

PhosphoSiteiP03953.

Expressioni

Gene expression databases

BgeeiP03953.
CleanExiMM_CFD.
ExpressionAtlasiP03953. baseline and differential.
GenevestigatoriP03953.

Interactioni

Protein-protein interaction databases

IntActiP03953. 2 interactions.
MINTiMINT-4090656.
STRINGi10090.ENSMUSP00000056836.

Structurei

3D structure databases

ProteinModelPortaliP03953.
SMRiP03953. Positions 26-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 254229Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP03953.
KOiK01334.
OMAiNMMCAES.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP03953.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P03953-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHSSVYFVAL VILGAAVCAA QPRGRILGGQ EAAAHARPYM ASVQVNGTHV
60 70 80 90 100
CGGTLLDEQW VLSAAHCMDG VTDDDSVQVL LGAHSLSAPE PYKRWYDVQS
110 120 130 140 150
VVPHPGSRPD SLEDDLILFK LSQNASLGPH VRPLPLQYED KEVEPGTLCD
160 170 180 190 200
VAGWGVVTHA GRRPDVLHQL RVSIMNRTTC NLRTYHDGVV TINMMCAESN
210 220 230 240 250
RRDTCRGDSG SPLVCGDAVE GVVTWGSRVC GNGKKPGVYT RVSSYRMWIE

NITNGNMTS
Length:259
Mass (Da):28,057
Last modified:October 23, 1986 - v1
Checksum:i8C3A952561247DF9
GO
Isoform 2 (identifier: P03953-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     20-20: Missing.

Show »
Length:258
Mass (Da):27,986
Checksum:i85E1F17A26ECF7EC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei20 – 201Missing in isoform 2. CuratedVSP_005382

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11768 mRNA. Translation: AAA40486.1.
X04673 Genomic DNA. Translation: CAA28378.1.
X04673 Genomic DNA. Translation: CAA28379.1.
M13386 Genomic DNA. Translation: AAA37262.1.
CCDSiCCDS23995.1. [P03953-1]
PIRiC25952. WMMS28.
RefSeqiNP_001278844.1. NM_001291915.1. [P03953-2]
NP_038487.1. NM_013459.3. [P03953-1]
UniGeneiMm.4407.

Genome annotation databases

EnsembliENSMUST00000061653; ENSMUSP00000056836; ENSMUSG00000061780. [P03953-1]
GeneIDi11537.
KEGGimmu:11537.
UCSCiuc007gaj.2. mouse. [P03953-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11768 mRNA. Translation: AAA40486.1.
X04673 Genomic DNA. Translation: CAA28378.1.
X04673 Genomic DNA. Translation: CAA28379.1.
M13386 Genomic DNA. Translation: AAA37262.1.
CCDSiCCDS23995.1. [P03953-1]
PIRiC25952. WMMS28.
RefSeqiNP_001278844.1. NM_001291915.1. [P03953-2]
NP_038487.1. NM_013459.3. [P03953-1]
UniGeneiMm.4407.

3D structure databases

ProteinModelPortaliP03953.
SMRiP03953. Positions 26-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03953. 2 interactions.
MINTiMINT-4090656.
STRINGi10090.ENSMUSP00000056836.

Protein family/group databases

MEROPSiS01.191.

PTM databases

PhosphoSiteiP03953.

Proteomic databases

MaxQBiP03953.
PaxDbiP03953.
PRIDEiP03953.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061653; ENSMUSP00000056836; ENSMUSG00000061780. [P03953-1]
GeneIDi11537.
KEGGimmu:11537.
UCSCiuc007gaj.2. mouse. [P03953-1]

Organism-specific databases

CTDi1675.
MGIiMGI:87931. Cfd.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP03953.
KOiK01334.
OMAiNMMCAES.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP03953.
TreeFamiTF333630.

Enzyme and pathway databases

ReactomeiREACT_218037. Alternative complement activation.

Miscellaneous databases

ChiTaRSiCfd. mouse.
NextBioi278986.
PROiP03953.
SOURCEiSearch...

Gene expression databases

BgeeiP03953.
CleanExiMM_CFD.
ExpressionAtlasiP03953. baseline and differential.
GenevestigatoriP03953.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of three genes participating in the adipose differentiation of 3T3 cells."
    Phillips M., Djian P., Green H.
    J. Biol. Chem. 261:10821-10827(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Adipsin, the adipocyte serine protease: gene structure and control of expression by tumor necrosis factor."
    Min H.Y., Spiegelman B.M.
    Nucleic Acids Res. 14:8879-8892(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
  3. "A developmentally regulated mRNA from 3T3 adipocytes encodes a novel serine protease homologue."
    Cook K.S., Groves D.L., Min H.Y., Spiegelman B.M.
    Proc. Natl. Acad. Sci. U.S.A. 82:6480-6484(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss white.
  4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-124.
    Strain: C57BL/6.
    Tissue: Plasma.
  5. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-176.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiCFAD_MOUSE
AccessioniPrimary (citable) accession number: P03953
Secondary accession number(s): Q61280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: October 23, 1986
Last modified: February 4, 2015
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.