P03953 (CFAD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Complement factor D
EC=3.4.21.46

Alternative name(s):
28 kDa adipocyte protein
Adipsin
C3 convertase activator
Properdin factor D

Gene names

Name:	Cfd
Synonyms:	Adn, Df

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Catalytic activity	Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.
Subcellular location	Secreted.
Post-translational modification	N-glycosylated. Ref.4 Ref.5
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Complement alternate pathway Immunity Innate immunity
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	complement activation, alternative pathway Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from direct assay. Source: MGI
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P03953-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P03953-2)

The sequence of this isoform differs from the canonical sequence as follows:
     20-20: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 25	5	Activation peptide Potential		PRO_0000027562
	Chain	26 – 259	234	Complement factor D		PRO_0000027563
Regions
	Domain	26 – 254	229	Peptidase S1
Sites
	Active site	66	1	Charge relay system
	Active site	115	1	Charge relay system
	Active site	209	1	Charge relay system
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	124	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	176	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	251	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	149 ↔ 215		By similarity
	Disulfide bond	180 ↔ 196		By similarity
	Disulfide bond	205 ↔ 230		By similarity
Natural variations
	Alternative sequence	20	1	Missing in isoform 2.		VSP_005382

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 23, 1986. Version 1. Checksum: 8C3A952561247DF9	FASTA	259	28,057
	10 20 30 40 50 60 MHSSVYFVAL VILGAAVCAA QPRGRILGGQ EAAAHARPYM ASVQVNGTHV CGGTLLDEQW 70 80 90 100 110 120 VLSAAHCMDG VTDDDSVQVL LGAHSLSAPE PYKRWYDVQS VVPHPGSRPD SLEDDLILFK 130 140 150 160 170 180 LSQNASLGPH VRPLPLQYED KEVEPGTLCD VAGWGVVTHA GRRPDVLHQL RVSIMNRTTC 190 200 210 220 230 240 NLRTYHDGVV TINMMCAESN RRDTCRGDSG SPLVCGDAVE GVVTWGSRVC GNGKKPGVYT 250 RVSSYRMWIE NITNGNMTS
	Isoform 2 [UniParc]. Checksum: 85E1F17A26ECF7EC	FASTA	258	27,986
	10 20 30 40 50 60 MHSSVYFVAL VILGAAVCAQ PRGRILGGQE AAAHARPYMA SVQVNGTHVC GGTLLDEQWV 70 80 90 100 110 120 LSAAHCMDGV TDDDSVQVLL GAHSLSAPEP YKRWYDVQSV VPHPGSRPDS LEDDLILFKL 130 140 150 160 170 180 SQNASLGPHV RPLPLQYEDK EVEPGTLCDV AGWGVVTHAG RRPDVLHQLR VSIMNRTTCN 190 200 210 220 230 240 LRTYHDGVVT INMMCAESNR RDTCRGDSGS PLVCGDAVEG VVTWGSRVCG NGKKPGVYTR 250 VSSYRMWIEN ITNGNMTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of three genes participating in the adipose differentiation of 3T3 cells." Phillips M., Djian P., Green H. J. Biol. Chem. 261:10821-10827(1986) [PubMed: 3015943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Adipsin, the adipocyte serine protease: gene structure and control of expression by tumor necrosis factor." Min H.Y., Spiegelman B.M. Nucleic Acids Res. 14:8879-8892(1986) [PubMed: 3024123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
[3]	"A developmentally regulated mRNA from 3T3 adipocytes encodes a novel serine protease homologue." Cook K.S., Groves D.L., Min H.Y., Spiegelman B.M. Proc. Natl. Acad. Sci. U.S.A. 82:6480-6484(1985) [PubMed: 3901003] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss white.
[4]	"Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res. 5:2438-2447(2006) [PubMed: 16944957] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-124, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
[5]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed: 17330941] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-176, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11768 mRNA. Translation: AAA40486.1. X04673 Genomic DNA. Translation: CAA28378.1. X04673 Genomic DNA. Translation: CAA28379.1. M13386 Genomic DNA. Translation: AAA37262.1.
IPI	IPI00116945. IPI00224671.
PIR	WMMS28. C25952.
RefSeq	NP_038487.1. NM_013459.2.
UniGene	Mm.4407.
3D structure databases
ProteinModelPortal	P03953.
SMR	P03953. Positions 26-252.
ModBase	Search...
Protein-protein interaction databases
STRING	P03953.
Protein family/group databases
MEROPS	S01.191.
Proteomic databases
PRIDE	P03953.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000061653; ENSMUSP00000056836; ENSMUSG00000061780.
GeneID	11537.
KEGG	mmu:11537.
Organism-specific databases
CTD	1675.
MGI	MGI:87931. Cfd.
Phylogenomic databases
eggNOG	roNOG16853.
HOGENOM	HBG755338.
HOVERGEN	HBG013304.
InParanoid	P03953.
OMA	NMMCAES.
OrthoDB	EOG4FXR89.
PhylomeDB	P03953.
Gene expression databases
ArrayExpress	P03953.
Bgee	P03953.
CleanEx	MM_CFD.
Genevestigator	P03953.
GermOnline	ENSMUSG00000061780. Mus musculus.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
KO	K01334.
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	278986.
SOURCE	Search...

Entry information

Entry name

CFAD_MOUSE

Accession

Primary (citable) accession number: P03953
Secondary accession number(s): Q61280

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
Last sequence update:	October 23, 1986
Last modified:	November 16, 2011
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program