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P03953

- CFAD_MOUSE

UniProt

P03953 - CFAD_MOUSE

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Protein
Complement factor D
Gene
Cfd, Adn, Df
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Catalytic activityi

Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Charge relay system
Active sitei115 – 1151Charge relay system
Active sitei209 – 2091Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. complement activation, alternative pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement alternate pathway, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_218037. Alternative complement activation.

Protein family/group databases

MEROPSiS01.191.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement factor D (EC:3.4.21.46)
Alternative name(s):
28 kDa adipocyte protein
Adipsin
C3 convertase activator
Properdin factor D
Gene namesi
Name:Cfd
Synonyms:Adn, Df
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:87931. Cfd.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Propeptidei21 – 255Activation peptide Reviewed prediction
PRO_0000027562
Chaini26 – 259234Complement factor D
PRO_0000027563Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi51 ↔ 67 By similarity
Glycosylationi124 – 1241N-linked (GlcNAc...)1 Publication
Disulfide bondi149 ↔ 215 By similarity
Glycosylationi176 – 1761N-linked (GlcNAc...)1 Publication
Disulfide bondi180 ↔ 196 By similarity
Disulfide bondi205 ↔ 230 By similarity
Glycosylationi251 – 2511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi256 – 2561N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP03953.
PaxDbiP03953.
PRIDEiP03953.

PTM databases

PhosphoSiteiP03953.

Expressioni

Gene expression databases

ArrayExpressiP03953.
BgeeiP03953.
CleanExiMM_CFD.
GenevestigatoriP03953.

Interactioni

Protein-protein interaction databases

IntActiP03953. 2 interactions.
MINTiMINT-4090656.
STRINGi10090.ENSMUSP00000056836.

Structurei

3D structure databases

ProteinModelPortaliP03953.
SMRiP03953. Positions 26-252.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 254229Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP03953.
KOiK01334.
OMAiNMMCAES.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP03953.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P03953-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MHSSVYFVAL VILGAAVCAA QPRGRILGGQ EAAAHARPYM ASVQVNGTHV    50
CGGTLLDEQW VLSAAHCMDG VTDDDSVQVL LGAHSLSAPE PYKRWYDVQS 100
VVPHPGSRPD SLEDDLILFK LSQNASLGPH VRPLPLQYED KEVEPGTLCD 150
VAGWGVVTHA GRRPDVLHQL RVSIMNRTTC NLRTYHDGVV TINMMCAESN 200
RRDTCRGDSG SPLVCGDAVE GVVTWGSRVC GNGKKPGVYT RVSSYRMWIE 250
NITNGNMTS 259
Length:259
Mass (Da):28,057
Last modified:October 23, 1986 - v1
Checksum:i8C3A952561247DF9
GO
Isoform 2 (identifier: P03953-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     20-20: Missing.

Show »
Length:258
Mass (Da):27,986
Checksum:i85E1F17A26ECF7EC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei20 – 201Missing in isoform 2.
VSP_005382

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11768 mRNA. Translation: AAA40486.1.
X04673 Genomic DNA. Translation: CAA28378.1.
X04673 Genomic DNA. Translation: CAA28379.1.
M13386 Genomic DNA. Translation: AAA37262.1.
CCDSiCCDS23995.1. [P03953-1]
PIRiC25952. WMMS28.
RefSeqiNP_001278844.1. NM_001291915.1. [P03953-2]
NP_038487.1. NM_013459.3. [P03953-1]
UniGeneiMm.4407.

Genome annotation databases

EnsembliENSMUST00000061653; ENSMUSP00000056836; ENSMUSG00000061780. [P03953-1]
GeneIDi11537.
KEGGimmu:11537.
UCSCiuc007gaj.2. mouse. [P03953-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11768 mRNA. Translation: AAA40486.1 .
X04673 Genomic DNA. Translation: CAA28378.1 .
X04673 Genomic DNA. Translation: CAA28379.1 .
M13386 Genomic DNA. Translation: AAA37262.1 .
CCDSi CCDS23995.1. [P03953-1 ]
PIRi C25952. WMMS28.
RefSeqi NP_001278844.1. NM_001291915.1. [P03953-2 ]
NP_038487.1. NM_013459.3. [P03953-1 ]
UniGenei Mm.4407.

3D structure databases

ProteinModelPortali P03953.
SMRi P03953. Positions 26-252.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P03953. 2 interactions.
MINTi MINT-4090656.
STRINGi 10090.ENSMUSP00000056836.

Protein family/group databases

MEROPSi S01.191.

PTM databases

PhosphoSitei P03953.

Proteomic databases

MaxQBi P03953.
PaxDbi P03953.
PRIDEi P03953.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000061653 ; ENSMUSP00000056836 ; ENSMUSG00000061780 . [P03953-1 ]
GeneIDi 11537.
KEGGi mmu:11537.
UCSCi uc007gaj.2. mouse. [P03953-1 ]

Organism-specific databases

CTDi 1675.
MGIi MGI:87931. Cfd.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P03953.
KOi K01334.
OMAi NMMCAES.
OrthoDBi EOG7MKW6Q.
PhylomeDBi P03953.
TreeFami TF333630.

Enzyme and pathway databases

Reactomei REACT_218037. Alternative complement activation.

Miscellaneous databases

ChiTaRSi CFD. mouse.
NextBioi 278986.
PROi P03953.
SOURCEi Search...

Gene expression databases

ArrayExpressi P03953.
Bgeei P03953.
CleanExi MM_CFD.
Genevestigatori P03953.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of three genes participating in the adipose differentiation of 3T3 cells."
    Phillips M., Djian P., Green H.
    J. Biol. Chem. 261:10821-10827(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Adipsin, the adipocyte serine protease: gene structure and control of expression by tumor necrosis factor."
    Min H.Y., Spiegelman B.M.
    Nucleic Acids Res. 14:8879-8892(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
  3. "A developmentally regulated mRNA from 3T3 adipocytes encodes a novel serine protease homologue."
    Cook K.S., Groves D.L., Min H.Y., Spiegelman B.M.
    Proc. Natl. Acad. Sci. U.S.A. 82:6480-6484(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss white.
  4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-124.
    Strain: C57BL/6.
    Tissue: Plasma.
  5. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-176.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiCFAD_MOUSE
AccessioniPrimary (citable) accession number: P03953
Secondary accession number(s): Q61280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: October 23, 1986
Last modified: September 3, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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