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P03950

- ANGI_HUMAN

UniProt

P03950 - ANGI_HUMAN

Protein

Angiogenin

Gene

ANG

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei37 – 371Proton acceptor
    Active sitei138 – 1381Proton donor

    GO - Molecular functioni

    1. actin binding Source: UniProtKB
    2. copper ion binding Source: UniProtKB
    3. DNA binding Source: UniProtKB
    4. endonuclease activity Source: UniProtKB
    5. endoribonuclease activity, producing 3'-phosphomonoesters Source: InterPro
    6. heparin binding Source: UniProtKB
    7. peptide binding Source: UniProtKB
    8. protein binding Source: UniProtKB
    9. receptor binding Source: UniProtKB
    10. ribonuclease activity Source: UniProtKB
    11. rRNA binding Source: UniProtKB

    GO - Biological processi

    1. actin filament polymerization Source: UniProtKB
    2. activation of phospholipase A2 activity Source: UniProtKB
    3. activation of phospholipase C activity Source: UniProtKB
    4. activation of protein kinase B activity Source: UniProtKB
    5. angiogenesis Source: UniProtKB
    6. cell communication Source: UniProtKB
    7. cell death Source: UniProtKB-KW
    8. cell migration Source: UniProtKB
    9. diacylglycerol biosynthetic process Source: UniProtKB
    10. homeostatic process Source: UniProtKB
    11. negative regulation of smooth muscle cell proliferation Source: UniProtKB
    12. negative regulation of translation Source: UniProtKB-KW
    13. nucleic acid phosphodiester bond hydrolysis Source: GOC
    14. oocyte maturation Source: UniProtKB
    15. ovarian follicle development Source: UniProtKB
    16. placenta development Source: UniProtKB
    17. positive regulation of endothelial cell proliferation Source: UniProtKB
    18. positive regulation of phosphorylation Source: UniProtKB
    19. positive regulation of protein secretion Source: UniProtKB
    20. response to hormone Source: UniProtKB
    21. response to hypoxia Source: UniProtKB
    22. RNA phosphodiester bond hydrolysis Source: GOC
    23. rRNA transcription Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Endonuclease, Hydrolase, Nuclease, Protein synthesis inhibitor

    Keywords - Biological processi

    Angiogenesis, Differentiation, Stress response

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Angiogenin (EC:3.1.27.-)
    Alternative name(s):
    Ribonuclease 5
    Short name:
    RNase 5
    Gene namesi
    Name:ANG
    Synonyms:RNASE5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:483. ANG.

    Subcellular locationi

    Secretedextracellular spaceextracellular matrixbasement membrane. Nucleusnucleolus
    Note: Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.

    GO - Cellular componenti

    1. angiogenin-PRI complex Source: UniProtKB
    2. basal lamina Source: UniProtKB
    3. extracellular space Source: UniProtKB
    4. extracellular vesicular exosome Source: UniProt
    5. growth cone Source: UniProtKB
    6. neuronal cell body Source: UniProtKB
    7. nucleolus Source: UniProtKB
    8. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Basement membrane, Extracellular matrix, Nucleus, Secreted

    Pathology & Biotechi

    Involvement in diseasei

    Amyotrophic lateral sclerosis 9 (ALS9) [MIM:611895]: A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.5 Publications
    Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti12 – 121F → S in ALS9. 1 Publication
    VAR_044145
    Natural varianti20 – 201P → S in ALS9. 2 Publications
    VAR_044146
    Natural varianti36 – 361Q → L in ALS9; reduced ribonucleolytic activity; low angiogenic activity; reduced mitogenic activity; wild type far-UV CD spectra. 1 Publication
    VAR_044147
    Natural varianti41 – 411K → E in ALS9; reduced ribonucleolytic activity. 1 Publication
    VAR_044148
    Natural varianti41 – 411K → I in ALS9; loss of angiogenic activity; reduced ribonucleolytic activity; retains nuclear translocation. 2 Publications
    VAR_044149
    Natural varianti52 – 521S → N in ALS9; loss of angiogenic activity; reduced ribonucleolytic activity; unable to translocate to the nucleus. 1 Publication
    VAR_044150
    Natural varianti55 – 551R → K in ALS9; marginally reduced ribonucleolytic activity; wild type far-UV CD spectra. 1 Publication
    VAR_044151
    Natural varianti63 – 631C → W in ALS9; reduced ribonucleolytic activity; low angiogenic activity; reduced mitogenic activity; reduced thermal stability. 1 Publication
    VAR_044152
    Natural varianti64 – 641K → I in ALS9; reduced ribonucleolytic activity; low angiogenic activity; reduced mitogenic activity; moderate reduction of thermal stability. 2 Publications
    VAR_044153
    Natural varianti70 – 701I → V in some ALS9 patients; pathogenicity uncertain; reduced ribonucleolytic activity; moderate reduction of thermal stability. 2 Publications
    VAR_044154
    Natural varianti136 – 1361P → L in ALS9; loss of angiogenic activity; reduced ribonucleolytic activity; unable to translocate to the nucleus. 1 Publication
    VAR_044155
    Natural varianti137 – 1371V → I in ALS9. 1 Publication
    VAR_044156
    Natural varianti138 – 1381H → R in ALS9. 1 Publication
    VAR_044157

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi29 – 291R → A: Significantly decreases binding affinity for RNH1. 2 Publications
    Mutagenesisi32 – 321H → A: Significantly decreases binding affinity for RNH1. 2 Publications
    Mutagenesisi36 – 361Q → A: Slightly decreases binding affinity for RNH1. 2 Publications
    Mutagenesisi64 – 641K → Q: Significantly decreases binding affinity for RNH1. 3 Publications
    Mutagenesisi92 – 921N → A: Slightly decreases binding affinity for RNH1. 2 Publications
    Mutagenesisi109 – 1102GG → RR: Significantly decreases binding affinity for RNH1. 1 Publication
    Mutagenesisi132 – 1321E → A: Slightly decreases binding affinity for RNH1. 2 Publications
    Mutagenesisi140 – 1401D → H, S or A: 15- to 18-fold increase in RNase activity. 2 Publications
    Mutagenesisi141 – 1411Q → G: Over 18-fold increase in RNase activity. 2 Publications
    Mutagenesisi143 – 1442IF → AA: 3- to 5-fold increase in RNase activity. 1 Publication

    Keywords - Diseasei

    Amyotrophic lateral sclerosis, Disease mutation, Neurodegeneration

    Organism-specific databases

    MIMi611895. phenotype.
    Orphaneti803. Amyotrophic lateral sclerosis.
    PharmGKBiPA24790.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Chaini25 – 147123Angiogenin2 PublicationsPRO_0000030843Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei25 – 251Pyrrolidone carboxylic acid1 Publication
    Disulfide bondi50 ↔ 1051 Publication
    Disulfide bondi63 ↔ 1161 Publication
    Disulfide bondi81 ↔ 1311 Publication

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Proteomic databases

    PaxDbiP03950.
    PeptideAtlasiP03950.
    PRIDEiP03950.

    PTM databases

    PhosphoSiteiP03950.

    Expressioni

    Tissue specificityi

    Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.2 Publications

    Developmental stagei

    Low level expression in the developing fetus, increased in the neonate, and maximal in the adult.

    Gene expression databases

    BgeeiP03950.
    CleanExiHS_ANG.
    GenevestigatoriP03950.

    Interactioni

    Subunit structurei

    Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ACTN2P356094EBI-525291,EBI-77797
    FSTP198833EBI-525291,EBI-1571188
    RNH1P134892EBI-525291,EBI-1237106

    Protein-protein interaction databases

    BioGridi106780. 7 interactions.
    IntActiP03950. 7 interactions.
    STRINGi9606.ENSP00000336762.

    Structurei

    Secondary structure

    1
    147
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi28 – 3710
    Helixi47 – 5610
    Turni60 – 634
    Beta strandi65 – 706
    Helixi74 – 785
    Helixi79 – 813
    Turni83 – 853
    Beta strandi86 – 894
    Turni90 – 923
    Beta strandi93 – 986
    Beta strandi100 – 11011
    Beta strandi112 – 1154
    Beta strandi117 – 1259
    Beta strandi128 – 1325
    Beta strandi135 – 1395
    Helixi141 – 1444

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A4YX-ray2.00B/E25-147[»]
    1ANGX-ray2.40A25-147[»]
    1AWZNMR-A25-147[»]
    1B1EX-ray2.00A25-147[»]
    1B1IX-ray1.80A26-147[»]
    1B1JX-ray2.00A25-147[»]
    1GV7X-ray2.10A26-145[»]
    1H0DX-ray2.00C26-147[»]
    1H52X-ray2.00A25-147[»]
    1H53X-ray2.00A26-147[»]
    1HBYX-ray2.00A25-147[»]
    1K58X-ray2.70A25-147[»]
    1K59X-ray1.80A25-147[»]
    1K5AX-ray2.33A25-147[»]
    1K5BX-ray1.80A25-144[»]
    1UN3X-ray1.70A26-147[»]
    1UN4X-ray2.10A26-147[»]
    1UN5X-ray2.60A25-147[»]
    2ANGX-ray2.00A25-147[»]
    4AHDX-ray2.47A/B25-147[»]
    4AHEX-ray2.08A25-147[»]
    4AHFX-ray2.12A25-147[»]
    4AHGX-ray2.45A25-147[»]
    4AHHX-ray2.50A25-147[»]
    4AHIX-ray2.80A25-147[»]
    4AHJX-ray2.03A25-147[»]
    4AHKX-ray1.97A/B25-147[»]
    4AHLX-ray2.05A25-147[»]
    4AHMX-ray1.96A25-147[»]
    4AHNX-ray2.98A25-147[»]
    4AOHX-ray1.04A24-147[»]
    4B36X-ray1.76A/B25-147[»]
    ProteinModelPortaliP03950.
    SMRiP03950. Positions 24-145.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03950.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni64 – 685Substrate binding

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi55 – 595Nucleolar localization signal

    Sequence similaritiesi

    Belongs to the pancreatic ribonuclease family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG283332.
    HOGENOMiHOG000276883.
    HOVERGENiHBG008396.
    InParanoidiP03950.
    KOiK16631.
    OMAiPCKYRAT.
    OrthoDBiEOG7J1826.
    PhylomeDBiP03950.
    TreeFamiTF333393.

    Family and domain databases

    Gene3Di3.10.130.10. 1 hit.
    InterProiIPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    [Graphical view]
    PANTHERiPTHR11437. PTHR11437. 1 hit.
    PfamiPF00074. RnaseA. 1 hit.
    [Graphical view]
    PRINTSiPR00794. RIBONUCLEASE.
    ProDomiPD000535. RNaseA. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00092. RNAse_Pc. 1 hit.
    [Graphical view]
    SUPFAMiSSF54076. SSF54076. 1 hit.
    PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P03950-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVMGLGVLLL VFVLGLGLTP PTLAQDNSRY THFLTQHYDA KPQGRDDRYC    50
    ESIMRRRGLT SPCKDINTFI HGNKRSIKAI CENKNGNPHR ENLRISKSSF 100
    QVTTCKLHGG SPWPPCQYRA TAGFRNVVVA CENGLPVHLD QSIFRRP 147
    Length:147
    Mass (Da):16,550
    Last modified:October 23, 1986 - v1
    Checksum:i9C462DA3C8D39ACC
    GO

    Sequence cautioni

    The sequence AAH20704.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti59 – 591L → P in AAH62698. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti12 – 121F → S in ALS9. 1 Publication
    VAR_044145
    Natural varianti20 – 201P → S in ALS9. 2 Publications
    VAR_044146
    Natural varianti36 – 361Q → L in ALS9; reduced ribonucleolytic activity; low angiogenic activity; reduced mitogenic activity; wild type far-UV CD spectra. 1 Publication
    VAR_044147
    Natural varianti41 – 411K → E in ALS9; reduced ribonucleolytic activity. 1 Publication
    VAR_044148
    Natural varianti41 – 411K → I in ALS9; loss of angiogenic activity; reduced ribonucleolytic activity; retains nuclear translocation. 2 Publications
    VAR_044149
    Natural varianti52 – 521S → N in ALS9; loss of angiogenic activity; reduced ribonucleolytic activity; unable to translocate to the nucleus. 1 Publication
    VAR_044150
    Natural varianti55 – 551R → K in ALS9; marginally reduced ribonucleolytic activity; wild type far-UV CD spectra. 1 Publication
    VAR_044151
    Natural varianti63 – 631C → W in ALS9; reduced ribonucleolytic activity; low angiogenic activity; reduced mitogenic activity; reduced thermal stability. 1 Publication
    VAR_044152
    Natural varianti64 – 641K → I in ALS9; reduced ribonucleolytic activity; low angiogenic activity; reduced mitogenic activity; moderate reduction of thermal stability. 2 Publications
    VAR_044153
    Natural varianti70 – 701I → V in some ALS9 patients; pathogenicity uncertain; reduced ribonucleolytic activity; moderate reduction of thermal stability. 2 Publications
    VAR_044154
    Natural varianti84 – 841K → E.1 Publication
    Corresponds to variant rs17560 [ dbSNP | Ensembl ].
    VAR_013148
    Natural varianti136 – 1361P → L in ALS9; loss of angiogenic activity; reduced ribonucleolytic activity; unable to translocate to the nucleus. 1 Publication
    VAR_044155
    Natural varianti137 – 1371V → I in ALS9. 1 Publication
    VAR_044156
    Natural varianti138 – 1381H → R in ALS9. 1 Publication
    VAR_044157

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11567 Genomic DNA. Translation: AAA51678.1.
    AF449647 Genomic DNA. Translation: AAL67710.1.
    AF449648 Genomic DNA. Translation: AAL67711.1.
    AF449649 Genomic DNA. Translation: AAL67712.1.
    AF449650 Genomic DNA. Translation: AAL67713.1.
    AF449651 Genomic DNA. Translation: AAL67714.1.
    FJ236304 mRNA. Translation: ACI45236.1.
    CR407633 mRNA. Translation: CAG28561.1.
    AK313989 mRNA. Translation: BAG36701.1.
    CH471078 Genomic DNA. Translation: EAW66450.1.
    CH471078 Genomic DNA. Translation: EAW66451.1.
    BC020704 mRNA. Translation: AAH20704.1. Different initiation.
    BC054880 mRNA. Translation: AAH54880.1.
    BC062698 mRNA. Translation: AAH62698.1.
    CCDSiCCDS9554.1.
    PIRiA90498. NRHUAG.
    RefSeqiNP_001091046.1. NM_001097577.2.
    NP_001136.1. NM_001145.4.
    UniGeneiHs.283749.

    Genome annotation databases

    EnsembliENST00000336811; ENSP00000336762; ENSG00000214274.
    ENST00000397990; ENSP00000381077; ENSG00000214274.
    GeneIDi283.
    KEGGihsa:283.
    UCSCiuc001vxw.4. human.

    Polymorphism databases

    DMDMi113873.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    R&D Systems' cytokine mini-reviews: Angiogenin
    Functional Glycomics Gateway - Glycan Binding

    Angiogenin

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11567 Genomic DNA. Translation: AAA51678.1 .
    AF449647 Genomic DNA. Translation: AAL67710.1 .
    AF449648 Genomic DNA. Translation: AAL67711.1 .
    AF449649 Genomic DNA. Translation: AAL67712.1 .
    AF449650 Genomic DNA. Translation: AAL67713.1 .
    AF449651 Genomic DNA. Translation: AAL67714.1 .
    FJ236304 mRNA. Translation: ACI45236.1 .
    CR407633 mRNA. Translation: CAG28561.1 .
    AK313989 mRNA. Translation: BAG36701.1 .
    CH471078 Genomic DNA. Translation: EAW66450.1 .
    CH471078 Genomic DNA. Translation: EAW66451.1 .
    BC020704 mRNA. Translation: AAH20704.1 . Different initiation.
    BC054880 mRNA. Translation: AAH54880.1 .
    BC062698 mRNA. Translation: AAH62698.1 .
    CCDSi CCDS9554.1.
    PIRi A90498. NRHUAG.
    RefSeqi NP_001091046.1. NM_001097577.2.
    NP_001136.1. NM_001145.4.
    UniGenei Hs.283749.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A4Y X-ray 2.00 B/E 25-147 [» ]
    1ANG X-ray 2.40 A 25-147 [» ]
    1AWZ NMR - A 25-147 [» ]
    1B1E X-ray 2.00 A 25-147 [» ]
    1B1I X-ray 1.80 A 26-147 [» ]
    1B1J X-ray 2.00 A 25-147 [» ]
    1GV7 X-ray 2.10 A 26-145 [» ]
    1H0D X-ray 2.00 C 26-147 [» ]
    1H52 X-ray 2.00 A 25-147 [» ]
    1H53 X-ray 2.00 A 26-147 [» ]
    1HBY X-ray 2.00 A 25-147 [» ]
    1K58 X-ray 2.70 A 25-147 [» ]
    1K59 X-ray 1.80 A 25-147 [» ]
    1K5A X-ray 2.33 A 25-147 [» ]
    1K5B X-ray 1.80 A 25-144 [» ]
    1UN3 X-ray 1.70 A 26-147 [» ]
    1UN4 X-ray 2.10 A 26-147 [» ]
    1UN5 X-ray 2.60 A 25-147 [» ]
    2ANG X-ray 2.00 A 25-147 [» ]
    4AHD X-ray 2.47 A/B 25-147 [» ]
    4AHE X-ray 2.08 A 25-147 [» ]
    4AHF X-ray 2.12 A 25-147 [» ]
    4AHG X-ray 2.45 A 25-147 [» ]
    4AHH X-ray 2.50 A 25-147 [» ]
    4AHI X-ray 2.80 A 25-147 [» ]
    4AHJ X-ray 2.03 A 25-147 [» ]
    4AHK X-ray 1.97 A/B 25-147 [» ]
    4AHL X-ray 2.05 A 25-147 [» ]
    4AHM X-ray 1.96 A 25-147 [» ]
    4AHN X-ray 2.98 A 25-147 [» ]
    4AOH X-ray 1.04 A 24-147 [» ]
    4B36 X-ray 1.76 A/B 25-147 [» ]
    ProteinModelPortali P03950.
    SMRi P03950. Positions 24-145.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 106780. 7 interactions.
    IntActi P03950. 7 interactions.
    STRINGi 9606.ENSP00000336762.

    Chemistry

    BindingDBi P03950.
    ChEMBLi CHEMBL5829.

    PTM databases

    PhosphoSitei P03950.

    Polymorphism databases

    DMDMi 113873.

    Proteomic databases

    PaxDbi P03950.
    PeptideAtlasi P03950.
    PRIDEi P03950.

    Protocols and materials databases

    DNASUi 283.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000336811 ; ENSP00000336762 ; ENSG00000214274 .
    ENST00000397990 ; ENSP00000381077 ; ENSG00000214274 .
    GeneIDi 283.
    KEGGi hsa:283.
    UCSCi uc001vxw.4. human.

    Organism-specific databases

    CTDi 283.
    GeneCardsi GC14P021152.
    GeneReviewsi ANG.
    HGNCi HGNC:483. ANG.
    MIMi 105850. gene.
    611895. phenotype.
    neXtProti NX_P03950.
    Orphaneti 803. Amyotrophic lateral sclerosis.
    PharmGKBi PA24790.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG283332.
    HOGENOMi HOG000276883.
    HOVERGENi HBG008396.
    InParanoidi P03950.
    KOi K16631.
    OMAi PCKYRAT.
    OrthoDBi EOG7J1826.
    PhylomeDBi P03950.
    TreeFami TF333393.

    Miscellaneous databases

    EvolutionaryTracei P03950.
    GeneWikii Angiogenin.
    GenomeRNAii 283.
    NextBioi 1141.
    PROi P03950.
    SOURCEi Search...

    Gene expression databases

    Bgeei P03950.
    CleanExi HS_ANG.
    Genevestigatori P03950.

    Family and domain databases

    Gene3Di 3.10.130.10. 1 hit.
    InterProi IPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    [Graphical view ]
    PANTHERi PTHR11437. PTHR11437. 1 hit.
    Pfami PF00074. RnaseA. 1 hit.
    [Graphical view ]
    PRINTSi PR00794. RIBONUCLEASE.
    ProDomi PD000535. RNaseA. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00092. RNAse_Pc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54076. SSF54076. 1 hit.
    PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the cDNA and gene for angiogenin, a human angiogenesis factor."
      Kurachi K., Davie E.W., Strydom D.J., Riordan J.F., Vallee B.L.
      Biochemistry 24:5494-5499(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Diversifying selection of the tumor-growth promoter angiogenin in primate evolution."
      Zhang J., Rosenberg H.F.
      Mol. Biol. Evol. 19:438-445(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-84.
    3. Li J., Wang H.
      Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "NEDO functional analysis of protein and research application project."
      Wakamatsu A., Yamamoto J., Kimura K., Kaida T., Tsuchiya K., Iida Y., Takayama Y., Murakawa K., Kanehori K., Andoh T., Kagawa N., Sato R., Kawamura Y., Tanaka S., Kisu Y., Sugano S., Goshima N., Nomura N., Isogai T.
      Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Small intestine.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    8. Cited for: PROTEIN SEQUENCE OF 25-147, PYROGLUTAMATE FORMATION AT GLN-25, DISULFIDE BONDS.
    9. "Tissue distribution and developmental expression of the messenger RNA encoding angiogenin."
      Weiner H.L., Weiner L.H., Swain J.L.
      Science 237:280-282(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    10. "Angiogenin is a cytotoxic, tRNA-specific ribonuclease in the RNase A superfamily."
      Saxena S.K., Rybak S.M., Davey R.T. Jr., Youle R.J., Ackerman E.J.
      J. Biol. Chem. 267:21982-21986(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Identification of the nucleolar targeting signal of human angiogenin."
      Moroianu J., Riordan J.F.
      Biochem. Biophys. Res. Commun. 203:1765-1772(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, NUCLEOLAR LOCALIZATION SIGNAL.
    12. "Site-specific mutagenesis reveals differences in the structural bases for tight binding of RNase inhibitor to angiogenin and RNase A."
      Chen C.Z., Shapiro R.
      Proc. Natl. Acad. Sci. U.S.A. 94:1761-1766(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF HIS-32; GLN-36; LYS-64; ASN-92 AND GLU-132.
    13. "Superadditive and subadditive effects of 'hot spot' mutations within the interfaces of placental ribonuclease inhibitor with angiogenin and ribonuclease A."
      Chen C.Z., Shapiro R.
      Biochemistry 38:9273-9285(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RNH1, MUTAGENESIS OF ARG-29 AND LYS-64.
    14. "The nuclear function of angiogenin in endothelial cells is related to rRNA production."
      Xu Z.P., Tsuji T., Riordan J.F., Hu G.F.
      Biochem. Biophys. Res. Commun. 294:287-292(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING.
    15. "Ribonuclease inhibitor regulates neovascularization by human angiogenin."
      Dickson K.A., Kang D.K., Kwon Y.S., Kim J.C., Leland P.A., Kim B.M., Chang S.I., Raines R.T.
      Biochemistry 48:3804-3806(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RNH1, MUTAGENESIS OF 109-GLY-GLY-110.
    16. "Angiogenin-induced tRNA fragments inhibit translation initiation."
      Ivanov P., Emara M.M., Villen J., Gygi S.P., Anderson P.
      Mol. Cell 43:613-623(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    17. "Crystal structure of human angiogenin reveals the structural basis for its functional divergence from ribonuclease."
      Acharya K.R., Shapiro R., Allen S.C., Riordan J.F., Vallee B.L.
      Proc. Natl. Acad. Sci. U.S.A. 91:2915-2919(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 25-147.
    18. "Molecular recognition of human angiogenin by placental ribonuclease inhibitor -- an X-ray crystallographic study at 2.0-A resolution."
      Papageorgiou A.C., Shapiro R., Acharya K.R.
      EMBO J. 16:5162-5177(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-147 IN COMPLEX WITH RNH1 AND SUBUNIT.
    19. "Refined crystal structures of native human angiogenin and two active site variants: implications for the unique functional properties of an enzyme involved in neovascularisation during tumour growth."
      Leonidas D.D., Shapiro R., Allen S.C., Subbarao G.V., Veluraja K., Acharya K.R.
      J. Mol. Biol. 285:1209-1233(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 26-147.
    20. "Binding of phosphate and pyrophosphate ions at the active site of human angiogenin as revealed by X-ray crystallography."
      Leonidas D.D., Chavali G.B., Jardine A.M., Li S., Shapiro R., Acharya K.R.
      Protein Sci. 10:1669-1676(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 26-147 OF MUTANT GLY-141 IN COMPLEX WITH PHOSPHATE AND PYROPHOSPHATE.
    21. "Crystallographic studies on the role of the C-terminal segment of human angiogenin in defining enzymatic potency."
      Leonidas D.D., Shapiro R., Subbarao G.V., Russo A., Acharya K.R.
      Biochemistry 41:2552-2562(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 26-147, MUTAGENESIS OF ASP-140; GLN-141 AND 143-ILE-PHE-144.
    22. "The crystal structure of human angiogenin in complex with an antitumor neutralizing antibody."
      Chavali G.B., Papageorgiou A.C., Olson K.A., Fett J.W., Hu G., Shapiro R., Acharya K.R.
      Structure 11:875-885(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 26-147.
    23. "Crystallographic studies on structural features that determine the enzymatic specificity and potency of human angiogenin: Thr44, Thr80, and residues 38-41."
      Holloway D.E., Chavali G.B., Hares M.C., Baker M.D., Subbarao G.V., Shapiro R., Acharya K.R.
      Biochemistry 43:1230-1241(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF MUTANTS ASP-68 AND ALA-104.
    24. "Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy -- characterization of histidine protonation states and pKa values."
      Lequin O., Thuering H., Robin M., Lallemand J.-Y.
      Eur. J. Biochem. 250:712-726(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    25. "Mechanisms of loss of functions of human angiogenin variants implicated in amyotrophic lateral sclerosis."
      Padhi A.K., Kumar H., Vasaikar S.V., Jayaram B., Gomes J.
      PLoS ONE 7:E32479-E32479(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.
    26. Cited for: VARIANT ALS9 ILE-64.
    27. Cited for: VARIANTS ALS9 LEU-36; ILE-41; GLU-41; LYS-55; TRP-63 AND ILE-64, VARIANT VAL-70.
    28. "Angiogenin loss-of-function mutations in amyotrophic lateral sclerosis."
      Wu D., Yu W., Kishikawa H., Folkerth R.D., Iafrate A.J., Shen Y., Xin W., Sims K., Hu G.-F.
      Ann. Neurol. 62:609-617(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS ALS9 SER-20; ILE-41; ASN-52 AND LEU-136, CHARACTERIZATION OF VARIANTS ALS9 ILE-41; ASN-52 AND LEU-136, TISSUE SPECIFICITY.
    29. "Characterization of human angiogenin variants implicated in amyotrophic lateral sclerosis."
      Crabtree B., Thiyagarajan N., Prior S.H., Wilson P., Iyer S., Ferns T., Shapiro R., Brew K., Subramanian V., Acharya K.R.
      Biochemistry 46:11810-11818(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF VARIANTS ALS9 LEU-36; ILE-41; GLU-41; LYS-55; TRP-63 AND ILE-64, CHARACTERIZATION OF VARIANT VAL-70.
    30. "Identification of new ANG gene mutations in a large cohort of Italian patients with amyotrophic lateral sclerosis."
      Gellera C., Colombrita C., Ticozzi N., Castellotti B., Bragato C., Ratti A., Taroni F., Silani V.
      Neurogenetics 9:33-40(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS ALS9 SER-12; SER-20; ILE-137 AND ARG-138, VARIANT VAL-70.
    31. Cited for: INVOLVEMENT IN ALS9.

    Entry informationi

    Entry nameiANGI_HUMAN
    AccessioniPrimary (citable) accession number: P03950
    Secondary accession number(s): Q05CV1
    , Q53X86, Q6P5T2, Q8WXE7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 1986
    Last sequence update: October 23, 1986
    Last modified: October 1, 2014
    This is version 174 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-3 is the initiator.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3