P03949 (ABL1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase abl-1
EC=2.7.10.2

Gene names

Name:	abl-1
ORF Names:	M79.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1224 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage checkpoint Inferred from genetic interaction PubMed 15273685. Source: WormBase defense response to Gram-negative bacterium Inferred from genetic interaction PubMed 21399680. Source: WormBase defense response to fungus Inferred from mutant phenotype PubMed 21399680. Source: WormBase negative regulation of DNA damage response, signal transduction by p53 class mediator Inferred from mutant phenotype PubMed 15273685. Source: WormBase negative regulation of apoptotic process Inferred from mutant phenotype PubMed 15273685. Source: WormBase negative regulation of cell migration Inferred from genetic interaction PubMed 19402756. Source: WormBase negative regulation of engulfment of apoptotic cell Inferred from genetic interaction PubMed 19402756. Source: WormBase response to ionizing radiation Inferred from mutant phenotype PubMed 15273685. Source: WormBase spermatogenesis Inferred from mutant phenotype PubMed 11076749. Source: WormBase
Cellular_component	cytoplasm Inferred from sequence or structural similarity PubMed 15273685. Source: WormBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from sequence or structural similarity PubMed 15273685. Source: WormBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
alp-1	G5EEL1	4	EBI-2315883,EBI-2315902
C05D10.4	Q11181	5	EBI-2315883,EBI-2316016
eps-8	G5ED33	4	EBI-2315883,EBI-2315916
M57.1	Q9TYX9	4	EBI-2315883,EBI-2315745
secs-1	Q18953	5	EBI-2315883,EBI-318539
swsn-1	G5EF87	4	EBI-2315883,EBI-311928
vab-3	G5EDS1	4	EBI-2315883,EBI-319610
zfp-3	O02174	4	EBI-2315883,EBI-2315779

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform a (identifier: P03949-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: P03949-2)

The sequence of this isoform differs from the canonical sequence as follows:
     54-63: Missing.

Isoform c (identifier: P03949-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-88: MGHSHSTGKE...SSTFRGSQSR → MIMNPCFRER...CFITATIDSL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1224	1224	Tyrosine-protein kinase abl-1		PRO_0000088055
Regions
	Domain	115 – 188	74	SH3
	Domain	194 – 284	91	SH2
	Domain	311 – 562	252	Protein kinase
	Nucleotide binding	317 – 325	9	ATP By similarity
	Nucleotide binding	385 – 391	7	ATP By similarity
	Motif	450 – 474	25	Kinase activation loop By similarity
Sites
	Active site	432	1	Proton acceptor By similarity
	Binding site	340	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 88	88	MGHSH…GSQSR → MIMNPCFRERSQKRTKKRAK NRAKSRSTMYLSKVGDSSLA MSRSLPSVALPMHYLHHKLA ESICFITATIDSL in isoform c.		VSP_004962
	Alternative sequence	54 – 63	10	Missing in isoform b.		VSP_004963

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified January 17, 2003. Version 4. Checksum: 56D8513DD2061071	FASTA	1,224	138,326
	10 20 30 40 50 60 MGHSHSTGKE INDNELFTCE DPVFDQPVAS PKSEISSKLA EEIERSKSPL ILEVSPRTPD 70 80 90 100 110 120 SVQMFRPTFD TFRPPNSDSS TFRGSQSRED LVACSSMNSV NNVHDMNTVS SSSSSSAPLF 130 140 150 160 170 180 VALYDFHGVG EEQLSLRKGD QVRILGYNKN NEWCEARLYS TRKNDASNQR RLGEIGWVPS 190 200 210 220 230 240 NFIAPYNSLD KYTWYHGKIS RSDSEAILGS GITGSFLVRE SETSIGQYTI SVRHDGRVFH 250 260 270 280 290 300 YRINVDNTEK MFITQEVKFR TLGELVHHHS VHADGLICLL MYPASKKDKG RGLFSLSPNA 310 320 330 340 350 360 PDEWELDRSE IIMHNKLGGG QYGDVYEGYW KRHDCTIAVK ALKEDAMPLH EFLAEAAIMK 370 380 390 400 410 420 DLHHKNLVRL LGVCTHEAPF YIITEFMCNG NLLEYLRRTD KSLLPPIILV QMASQIASGM 430 440 450 460 470 480 SYLEARHFIH RDLAARNCLV SEHNIVKIAD FGLARFMKED TYTAHAGAKF PIKWTAPEGL 490 500 510 520 530 540 AFNTFSSKSD VWAFGVLLWE IATYGMAPYP GVELSNVYGL LENGFRMDGP QGCPPSVYRL 550 560 570 580 590 600 MLQCWNWSPS DRPRFRDIHF NLENLISSNS LNDEVQKQLK KNNDKKLESD KRRSNVRERS 610 620 630 640 650 660 DSKSRHSSHH DRDRDRESLH SRNSNPEIPN RSFIRTDDSV SFFNPSTTSK VTSFRAQGPP 670 680 690 700 710 720 FPPPPQQNTK PKLLKSVLNS NARHASEEFE RNEQDDVVPL AEKNVRKAVT RLGGTMPKGQ 730 740 750 760 770 780 RIDAYLDSMR RVDSWKESTD ADNEGAGSSS LSRTVSNDSL DTLPLPDSMN SSTYVKMHPA 790 800 810 820 830 840 SGENVFLRQI RSKLKKRSET PELDHIDSDT ADETTKSEKS PFGSLNKSSI KYPIKNAPEF 850 860 870 880 890 900 SENHSRVSPV PVPPSRNASV SVRPDSKAED SSDETTKDVG MWGPKHAVTR KIEIVKNDSY 910 920 930 940 950 960 PNVEGELKAK IRNLRHVPKE ESNTSSQEDL PLDATDNTND SIIVIPRDEK AKVRQLVTQK 970 980 990 1000 1010 1020 VSPLQHHRPF SLQCPNNSTS SAISHSEHAD SSETSSLSGV YEERMKPELP RKRSNGDTKV 1030 1040 1050 1060 1070 1080 VPVTWIINGE KEPNGMARTK SLRDITSKFE QLGTASTIES KIEEAVPYRE HALEKKGTSK 1090 1100 1110 1120 1130 1140 RFSMLEGSNE LKHVVPPRKN RNQDESGSID EEPVSKDMIV SLLKVIQKEF VNLFNLASSE 1150 1160 1170 1180 1190 1200 ITDEKLQQFV IMADNVQKLH STCSVYAEQI SPHSKFRFKE LLSQLEIYNR QIKFSHNPRA 1210 1220 KPVDDKLKMA FQDCFDQIMR LVDR
	Isoform b [UniParc]. Checksum: B0535D6CC47604C1	FASTA	1,214	137,259
	10 20 30 40 50 60 MGHSHSTGKE INDNELFTCE DPVFDQPVAS PKSEISSKLA EEIERSKSPL ILEMFRPTFD 70 80 90 100 110 120 TFRPPNSDSS TFRGSQSRED LVACSSMNSV NNVHDMNTVS SSSSSSAPLF VALYDFHGVG 130 140 150 160 170 180 EEQLSLRKGD QVRILGYNKN NEWCEARLYS TRKNDASNQR RLGEIGWVPS NFIAPYNSLD 190 200 210 220 230 240 KYTWYHGKIS RSDSEAILGS GITGSFLVRE SETSIGQYTI SVRHDGRVFH YRINVDNTEK 250 260 270 280 290 300 MFITQEVKFR TLGELVHHHS VHADGLICLL MYPASKKDKG RGLFSLSPNA PDEWELDRSE 310 320 330 340 350 360 IIMHNKLGGG QYGDVYEGYW KRHDCTIAVK ALKEDAMPLH EFLAEAAIMK DLHHKNLVRL 370 380 390 400 410 420 LGVCTHEAPF YIITEFMCNG NLLEYLRRTD KSLLPPIILV QMASQIASGM SYLEARHFIH 430 440 450 460 470 480 RDLAARNCLV SEHNIVKIAD FGLARFMKED TYTAHAGAKF PIKWTAPEGL AFNTFSSKSD 490 500 510 520 530 540 VWAFGVLLWE IATYGMAPYP GVELSNVYGL LENGFRMDGP QGCPPSVYRL MLQCWNWSPS 550 560 570 580 590 600 DRPRFRDIHF NLENLISSNS LNDEVQKQLK KNNDKKLESD KRRSNVRERS DSKSRHSSHH 610 620 630 640 650 660 DRDRDRESLH SRNSNPEIPN RSFIRTDDSV SFFNPSTTSK VTSFRAQGPP FPPPPQQNTK 670 680 690 700 710 720 PKLLKSVLNS NARHASEEFE RNEQDDVVPL AEKNVRKAVT RLGGTMPKGQ RIDAYLDSMR 730 740 750 760 770 780 RVDSWKESTD ADNEGAGSSS LSRTVSNDSL DTLPLPDSMN SSTYVKMHPA SGENVFLRQI 790 800 810 820 830 840 RSKLKKRSET PELDHIDSDT ADETTKSEKS PFGSLNKSSI KYPIKNAPEF SENHSRVSPV 850 860 870 880 890 900 PVPPSRNASV SVRPDSKAED SSDETTKDVG MWGPKHAVTR KIEIVKNDSY PNVEGELKAK 910 920 930 940 950 960 IRNLRHVPKE ESNTSSQEDL PLDATDNTND SIIVIPRDEK AKVRQLVTQK VSPLQHHRPF 970 980 990 1000 1010 1020 SLQCPNNSTS SAISHSEHAD SSETSSLSGV YEERMKPELP RKRSNGDTKV VPVTWIINGE 1030 1040 1050 1060 1070 1080 KEPNGMARTK SLRDITSKFE QLGTASTIES KIEEAVPYRE HALEKKGTSK RFSMLEGSNE 1090 1100 1110 1120 1130 1140 LKHVVPPRKN RNQDESGSID EEPVSKDMIV SLLKVIQKEF VNLFNLASSE ITDEKLQQFV 1150 1160 1170 1180 1190 1200 IMADNVQKLH STCSVYAEQI SPHSKFRFKE LLSQLEIYNR QIKFSHNPRA KPVDDKLKMA 1210 FQDCFDQIMR LVDR
	Isoform c [UniParc]. Checksum: 733EA68DDB491076	FASTA	1,209	136,811
	10 20 30 40 50 60 MIMNPCFRER SQKRTKKRAK NRAKSRSTMY LSKVGDSSLA MSRSLPSVAL PMHYLHHKLA 70 80 90 100 110 120 ESICFITATI DSLEDLVACS SMNSVNNVHD MNTVSSSSSS SAPLFVALYD FHGVGEEQLS 130 140 150 160 170 180 LRKGDQVRIL GYNKNNEWCE ARLYSTRKND ASNQRRLGEI GWVPSNFIAP YNSLDKYTWY 190 200 210 220 230 240 HGKISRSDSE AILGSGITGS FLVRESETSI GQYTISVRHD GRVFHYRINV DNTEKMFITQ 250 260 270 280 290 300 EVKFRTLGEL VHHHSVHADG LICLLMYPAS KKDKGRGLFS LSPNAPDEWE LDRSEIIMHN 310 320 330 340 350 360 KLGGGQYGDV YEGYWKRHDC TIAVKALKED AMPLHEFLAE AAIMKDLHHK NLVRLLGVCT 370 380 390 400 410 420 HEAPFYIITE FMCNGNLLEY LRRTDKSLLP PIILVQMASQ IASGMSYLEA RHFIHRDLAA 430 440 450 460 470 480 RNCLVSEHNI VKIADFGLAR FMKEDTYTAH AGAKFPIKWT APEGLAFNTF SSKSDVWAFG 490 500 510 520 530 540 VLLWEIATYG MAPYPGVELS NVYGLLENGF RMDGPQGCPP SVYRLMLQCW NWSPSDRPRF 550 560 570 580 590 600 RDIHFNLENL ISSNSLNDEV QKQLKKNNDK KLESDKRRSN VRERSDSKSR HSSHHDRDRD 610 620 630 640 650 660 RESLHSRNSN PEIPNRSFIR TDDSVSFFNP STTSKVTSFR AQGPPFPPPP QQNTKPKLLK 670 680 690 700 710 720 SVLNSNARHA SEEFERNEQD DVVPLAEKNV RKAVTRLGGT MPKGQRIDAY LDSMRRVDSW 730 740 750 760 770 780 KESTDADNEG AGSSSLSRTV SNDSLDTLPL PDSMNSSTYV KMHPASGENV FLRQIRSKLK 790 800 810 820 830 840 KRSETPELDH IDSDTADETT KSEKSPFGSL NKSSIKYPIK NAPEFSENHS RVSPVPVPPS 850 860 870 880 890 900 RNASVSVRPD SKAEDSSDET TKDVGMWGPK HAVTRKIEIV KNDSYPNVEG ELKAKIRNLR 910 920 930 940 950 960 HVPKEESNTS SQEDLPLDAT DNTNDSIIVI PRDEKAKVRQ LVTQKVSPLQ HHRPFSLQCP 970 980 990 1000 1010 1020 NNSTSSAISH SEHADSSETS SLSGVYEERM KPELPRKRSN GDTKVVPVTW IINGEKEPNG 1030 1040 1050 1060 1070 1080 MARTKSLRDI TSKFEQLGTA STIESKIEEA VPYREHALEK KGTSKRFSML EGSNELKHVV 1090 1100 1110 1120 1130 1140 PPRKNRNQDE SGSIDEEPVS KDMIVSLLKV IQKEFVNLFN LASSEITDEK LQQFVIMADN 1150 1160 1170 1180 1190 1200 VQKLHSTCSV YAEQISPHSK FRFKELLSQL EIYNRQIKFS HNPRAKPVDD KLKMAFQDCF DQIMRLVDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"Isolation and characterization of Caenorhabditis elegans DNA sequences homologous to the v-abl oncogene." Goddard J.M., Weiland J.J., Capecchi M.R. Proc. Natl. Acad. Sci. U.S.A. 83:2172-2176(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 150-706.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z50806 Genomic DNA. Translation: CAA90691.2. Z50806 Genomic DNA. Translation: CAB60296.2. Z50806 Genomic DNA. Translation: CAB60297.2. M13235 Genomic DNA. Translation: AAA28129.1.
PIR	T23832.
RefSeq	NP_509777.2. NM_077376.6. NP_509778.2. NM_077377.4. NP_509779.2. NM_077378.2.
UniGene	Cel.19997.
3D structure databases
ProteinModelPortal	P03949.
SMR	P03949. Positions 119-618.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48409N.
IntAct	P03949. 19 interactions.
Proteomic databases
PaxDb	P03949.
PRIDE	P03949.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	M79.1a; M79.1a; M79.1.
GeneID	181261.
KEGG	cel:CELE_M79.1.
UCSC	M79.1b. c. elegans.
Organism-specific databases
CTD	181261.
WormBase	M79.1a; CE31570; WBGene00000018; abl-1. M79.1b; CE31571; WBGene00000018; abl-1. M79.1c; CE31572; WBGene00000018; abl-1.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00640000091347.
HOGENOM	HOG000017506.
InParanoid	P03949.
KO	K06619.
OMA	CTHEAPF.
Enzyme and pathway databases
BRENDA	2.7.10.2. 1045.
Gene expression databases
ArrayExpress	P03949.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR015015. F-actin_binding. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00109. TYRKINASE.
SMART	SM00808. FABD. 1 hit. SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	913152.

Entry information

Entry name

ABL1_CAEEL

Accession

Primary (citable) accession number: P03949
Secondary accession number(s): Q9U3A2, Q9U3A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
Last sequence update:	January 17, 2003
Last modified:	May 1, 2013
This is version 126 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project