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P03945

- COX1_NEUCR

UniProt

P03945 - COX1_NEUCR

Protein

Cytochrome c oxidase subunit 1

Gene

cox-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (14 Dec 2011)
      Previous versions | rss
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    Functioni

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi67 – 671Iron (heme A axial ligand)Curated
    Metal bindingi246 – 2461Copper BCurated
    Metal bindingi250 – 2501Copper BCurated
    Metal bindingi295 – 2951Copper BCurated
    Metal bindingi296 – 2961Copper BCurated
    Metal bindingi381 – 3811Iron (heme A3 axial ligand)Curated
    Metal bindingi383 – 3831Iron (heme A axial ligand)Curated

    GO - Molecular functioni

    1. cytochrome-c oxidase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. aerobic respiration Source: InterPro
    2. oxidative phosphorylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Heme, Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00705.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
    Alternative name(s):
    Cytochrome c oxidase polypeptide I
    Gene namesi
    Name:cox-1
    Synonyms:coi, cox1
    ORF Names:NCM025, NCU16016
    Encoded oniMitochondrion
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Mitochondrion

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: UniProtKB-SubCell
    3. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 557557Cytochrome c oxidase subunit 1PRO_0000183366Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki246 ↔ 2501'-histidyl-3'-tyrosine (His-Tyr)By similarity
    Lipidationi324 – 3241N6-myristoyl lysine1 Publication

    Post-translational modificationi

    The amino end of the mature protein may be Ser-3.

    Keywords - PTMi

    Lipoprotein, Myristate

    Structurei

    3D structure databases

    ProteinModelPortaliP03945.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei22 – 4221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei69 – 8921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei106 – 12621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei151 – 17121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei187 – 20721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei240 – 26021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei272 – 29221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei310 – 33021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei343 – 36321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei378 – 39821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei418 – 43821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei457 – 47721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    OrthoDBiEOG72C58S.

    Family and domain databases

    Gene3Di1.20.210.10. 1 hit.
    InterProiIPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    [Graphical view]
    PANTHERiPTHR10422. PTHR10422. 1 hit.
    PfamiPF00115. COX1. 1 hit.
    [Graphical view]
    PRINTSiPR01165. CYCOXIDASEI.
    SUPFAMiSSF81442. SSF81442. 1 hit.
    PROSITEiPS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03945-1 [UniParc]FASTAAdd to Basket

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    MSSISIWTER WFLSTNAKDI GVLYLIFALF SGLLGTAFSV LIRMELSGPG    50
    VQYIADNQLY NAIITAHAIL MIFFMVMPAL IGGFGNFLLP LLVGGPDMAF 100
    PRLNNISFWL LPPSLLLLVF SACIEGGAGT GWTIYPPLSG VQSHSGPSVD 150
    LAIFALHLSG VSSLLGSINF ITTIVNMRTP GIRLHKLALF GWAVVITAVL 200
    LLLSLPVLAG AITMLLTDRN FNTSFFETAG GGDPILFQHL FWFFGHPEVY 250
    ILIIPGFGII STTISAYSNK SVFGYIGMVY AMMSIGILGF IVWSHHMYTV 300
    GLDVDTRAYF TAATLIIAVP TGIKIFSWLA TCYGGSIRLT PSMLFALGFV 350
    FMFTIGGLSG VVLANASLDI AFHDTYYVVA HFHYVLSMGA VFAMFSGWYH 400
    WVPKILGLNY NMVLSKAQFW LLFIGVNLTF FPQHFLGLQG MPRRISDYPD 450
    AFSGWNLISS FGSIVSVVAS WLFLYIVYIQ LVQGEYAGRY PWSIPQFYTD 500
    SLRALLNRSY PSLEWSISSP PKPHSFVSLP LQSSSFFLSF FRLSSYGEQK 550
    EISGRQN 557
    Length:557
    Mass (Da):61,522
    Last modified:December 14, 2011 - v2
    Checksum:i8AC9618704E5C91D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti527 – 5271V → A in CAA25976. (PubMed:6327266)Curated
    Sequence conflicti527 – 5271V → A in CAA28195. (PubMed:2531370)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01850 Genomic DNA. Translation: CAA25976.1.
    M36958 Genomic DNA. No translation available.
    X14669 Genomic DNA. Translation: CAA32799.1.
    KC683708 Genomic DNA. Translation: AGG16005.1.
    X04512 Genomic DNA. Translation: CAA28195.1.
    PIRiA00469. ODNC1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01850 Genomic DNA. Translation: CAA25976.1 .
    M36958 Genomic DNA. No translation available.
    X14669 Genomic DNA. Translation: CAA32799.1 .
    KC683708 Genomic DNA. Translation: AGG16005.1 .
    X04512 Genomic DNA. Translation: CAA28195.1 .
    PIRi A00469. ODNC1.

    3D structure databases

    ProteinModelPortali P03945.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    OrthoDBi EOG72C58S.

    Enzyme and pathway databases

    UniPathwayi UPA00705 .

    Family and domain databases

    Gene3Di 1.20.210.10. 1 hit.
    InterProi IPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    [Graphical view ]
    PANTHERi PTHR10422. PTHR10422. 1 hit.
    Pfami PF00115. COX1. 1 hit.
    [Graphical view ]
    PRINTSi PR01165. CYCOXIDASEI.
    SUPFAMi SSF81442. SSF81442. 1 hit.
    PROSITEi PS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Subunit 1 of cytochrome oxidase from Neurospora crassa: nucleotide sequence of the coding gene and partial amino acid sequence of the protein."
      Burger G., Scriven C., Machleidt W., Werner S.
      EMBO J. 1:1385-1391(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 3-99; 234-285; 346-354; 443-489 AND 494-521.
    2. "The structure of the gene for subunit I of cytochrome c oxidase in Neurospora crassa mitochondria."
      de Jonge J.C., de Vries H.
      Curr. Genet. 7:21-28(1983)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "A group II intron in the Neurospora mitochondrial coI gene: nucleotide sequence and implications for splicing and molecular evolution."
      Field D.J., Sommerfield A., Saville B.J., Collins R.A.
      Nucleic Acids Res. 17:9087-9099(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Adiopodoume / FGSC 430.
    4. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    5. "Mitochondrial genetics of Neurospora."
      Kennell J.C., Collins R.A., Griffiths A.J.F., Nargang F.E.
      (In) Kueck U. (eds.); The Mycota II, Genetics and Biotechnology (2nd edition), pp.95-112, Springer-Verlag, Berlin-Heidelberg (2004)
      Cited for: GENOME REANNOTATION.
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    6. "Mitochondrial gene URFN of Neurospora crassa codes for a long polypeptide with highly repetitive structure."
      Burger G., Werner S.
      J. Mol. Biol. 191:589-599(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 519-557.
    7. "Cytochrome c oxidase in Neurospora crassa contains myristic acid covalently linked to subunit 1."
      Vassilev A.O., Plesofsky-Vig N., Brambl R.
      Proc. Natl. Acad. Sci. U.S.A. 92:8680-8684(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: MYRISTOYLATION AT LYS-324.

    Entry informationi

    Entry nameiCOX1_NEUCR
    AccessioniPrimary (citable) accession number: P03945
    Secondary accession number(s): M1RV30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 1986
    Last sequence update: December 14, 2011
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3