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Protein

Cytochrome c oxidase subunit 1

Gene

cox-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathway:ioxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Iron (heme A axial ligand)Curated
Metal bindingi246 – 2461Copper BCurated
Metal bindingi250 – 2501Copper BCurated
Metal bindingi295 – 2951Copper BCurated
Metal bindingi296 – 2961Copper BCurated
Metal bindingi381 – 3811Iron (heme A3 axial ligand)Curated
Metal bindingi383 – 3831Iron (heme A axial ligand)Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00705.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide I
Gene namesi
Name:cox-1
Synonyms:coi, cox1
ORF Names:NCM025, NCU16016
Encoded oniMitochondrion
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805 Componenti: Mitochondrion

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei22 – 4221HelicalSequence AnalysisAdd
BLAST
Transmembranei69 – 8921HelicalSequence AnalysisAdd
BLAST
Transmembranei106 – 12621HelicalSequence AnalysisAdd
BLAST
Transmembranei151 – 17121HelicalSequence AnalysisAdd
BLAST
Transmembranei187 – 20721HelicalSequence AnalysisAdd
BLAST
Transmembranei240 – 26021HelicalSequence AnalysisAdd
BLAST
Transmembranei272 – 29221HelicalSequence AnalysisAdd
BLAST
Transmembranei310 – 33021HelicalSequence AnalysisAdd
BLAST
Transmembranei343 – 36321HelicalSequence AnalysisAdd
BLAST
Transmembranei378 – 39821HelicalSequence AnalysisAdd
BLAST
Transmembranei418 – 43821HelicalSequence AnalysisAdd
BLAST
Transmembranei457 – 47721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Cytochrome c oxidase subunit 1PRO_0000183366Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki246 ↔ 2501'-histidyl-3'-tyrosine (His-Tyr)By similarity
Lipidationi324 – 3241N6-myristoyl lysine1 Publication

Post-translational modificationi

The amino end of the mature protein may be Ser-3.

Keywords - PTMi

Lipoprotein, Myristate

Structurei

3D structure databases

ProteinModelPortaliP03945.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiP03945.
OrthoDBiEOG72C58S.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03945-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSISIWTER WFLSTNAKDI GVLYLIFALF SGLLGTAFSV LIRMELSGPG
60 70 80 90 100
VQYIADNQLY NAIITAHAIL MIFFMVMPAL IGGFGNFLLP LLVGGPDMAF
110 120 130 140 150
PRLNNISFWL LPPSLLLLVF SACIEGGAGT GWTIYPPLSG VQSHSGPSVD
160 170 180 190 200
LAIFALHLSG VSSLLGSINF ITTIVNMRTP GIRLHKLALF GWAVVITAVL
210 220 230 240 250
LLLSLPVLAG AITMLLTDRN FNTSFFETAG GGDPILFQHL FWFFGHPEVY
260 270 280 290 300
ILIIPGFGII STTISAYSNK SVFGYIGMVY AMMSIGILGF IVWSHHMYTV
310 320 330 340 350
GLDVDTRAYF TAATLIIAVP TGIKIFSWLA TCYGGSIRLT PSMLFALGFV
360 370 380 390 400
FMFTIGGLSG VVLANASLDI AFHDTYYVVA HFHYVLSMGA VFAMFSGWYH
410 420 430 440 450
WVPKILGLNY NMVLSKAQFW LLFIGVNLTF FPQHFLGLQG MPRRISDYPD
460 470 480 490 500
AFSGWNLISS FGSIVSVVAS WLFLYIVYIQ LVQGEYAGRY PWSIPQFYTD
510 520 530 540 550
SLRALLNRSY PSLEWSISSP PKPHSFVSLP LQSSSFFLSF FRLSSYGEQK

EISGRQN
Length:557
Mass (Da):61,522
Last modified:December 14, 2011 - v2
Checksum:i8AC9618704E5C91D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti527 – 5271V → A in CAA25976 (PubMed:6327266).Curated
Sequence conflicti527 – 5271V → A in CAA28195 (PubMed:2531370).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01850 Genomic DNA. Translation: CAA25976.1.
M36958 Genomic DNA. No translation available.
X14669 Genomic DNA. Translation: CAA32799.1.
KC683708 Genomic DNA. Translation: AGG16005.1.
X04512 Genomic DNA. Translation: CAA28195.1.
PIRiA00469. ODNC1.
RefSeqiYP_009126717.1. NC_026614.1.

Genome annotation databases

GeneIDi23681570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01850 Genomic DNA. Translation: CAA25976.1.
M36958 Genomic DNA. No translation available.
X14669 Genomic DNA. Translation: CAA32799.1.
KC683708 Genomic DNA. Translation: AGG16005.1.
X04512 Genomic DNA. Translation: CAA28195.1.
PIRiA00469. ODNC1.
RefSeqiYP_009126717.1. NC_026614.1.

3D structure databases

ProteinModelPortaliP03945.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23681570.

Phylogenomic databases

InParanoidiP03945.
OrthoDBiEOG72C58S.

Enzyme and pathway databases

UniPathwayiUPA00705.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Subunit 1 of cytochrome oxidase from Neurospora crassa: nucleotide sequence of the coding gene and partial amino acid sequence of the protein."
    Burger G., Scriven C., Machleidt W., Werner S.
    EMBO J. 1:1385-1391(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 3-99; 234-285; 346-354; 443-489 AND 494-521.
  2. "The structure of the gene for subunit I of cytochrome c oxidase in Neurospora crassa mitochondria."
    de Jonge J.C., de Vries H.
    Curr. Genet. 7:21-28(1983)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A group II intron in the Neurospora mitochondrial coI gene: nucleotide sequence and implications for splicing and molecular evolution."
    Field D.J., Sommerfield A., Saville B.J., Collins R.A.
    Nucleic Acids Res. 17:9087-9099(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Adiopodoume / FGSC 430.
  4. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  5. "Mitochondrial genetics of Neurospora."
    Kennell J.C., Collins R.A., Griffiths A.J.F., Nargang F.E.
    (In) Kueck U. (eds.); The Mycota II, Genetics and Biotechnology (2nd edition), pp.95-112, Springer-Verlag, Berlin-Heidelberg (2004)
    Cited for: GENOME REANNOTATION.
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  6. "Mitochondrial gene URFN of Neurospora crassa codes for a long polypeptide with highly repetitive structure."
    Burger G., Werner S.
    J. Mol. Biol. 191:589-599(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 519-557.
  7. "Cytochrome c oxidase in Neurospora crassa contains myristic acid covalently linked to subunit 1."
    Vassilev A.O., Plesofsky-Vig N., Brambl R.
    Proc. Natl. Acad. Sci. U.S.A. 92:8680-8684(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MYRISTOYLATION AT LYS-324.

Entry informationi

Entry nameiCOX1_NEUCR
AccessioniPrimary (citable) accession number: P03945
Secondary accession number(s): M1RV30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: December 14, 2011
Last modified: July 22, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.