Reviewed, UniProtKB/Swiss-Prot P03942 (FER_THET8)

Names and origin

Protein names

Recommended name:
    Ferredoxin

Gene names

Ordered Locus Names:

TTHA0177

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	79 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
Cofactor	Binds 1 4Fe-4S cluster. Binds 1 3Fe-4S cluster.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2 Ref.3

Chain

2 – 79

78

Ferredoxin

PRO_0000159108

Regions

Domain

2 – 30

29

4Fe-4S ferredoxin-type 1

Domain

31 – 60

30

4Fe-4S ferredoxin-type 2

Sites

Metal binding

9

1

Iron-sulfur 1 (3Fe-4S)

Metal binding

17

1

Iron-sulfur 1 (3Fe-4S)

Metal binding

21

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

40

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

43

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

46

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

50

1

Iron-sulfur 1 (3Fe-4S)

Experimental info

Sequence conflict

7

1

E → Q AA sequence Ref.3

Secondary structure

1

.

.

.

.

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.

.

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.

.

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.

.

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.

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79

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Helix

7 – 9

3

Turn

10 – 12

3

Helix

16 – 20

5

Beta strand

26 – 28

3

Beta strand

30 – 35

6

Turn

37 – 39

3

Helix

46 – 49

4

Beta strand

55 – 57

3

Helix

58 – 60

3

Helix

63 – 66

4

Helix

67 – 75

9

Sequences

	Sequence		Length	Mass (Da)
	P03942-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 33BB84B159BC10A6	FASTA	79	8,818
	10 20 30 40 50 60 MPHVICEPCI GVKDQSCVEV CPVECIYDGG DQFYIHPEEC IDCGACVPAC PVNAIYPEED 70 VPEQWKSYIE KNRKLAGLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Purification, some properties and amino acid sequence of Thermus thermophilus HB8 ferredoxin." Sato S., Nakazawa K., Hon-Nami K., Oshima T. Biochim. Biophys. Acta 668:277-289(1981) [PubMed: 7225412] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-79.
[3]	"New insights into the thermostability of bacterial ferredoxins: high-resolution crystal structure of the seven-iron ferredoxin from Thermus thermophilus." Macedo-Ribeiro S., Martins B.M., Pereira P.J., Buse G., Huber R., Soulimane T. J. Biol. Inorg. Chem. 6:663-674(2001) [PubMed: 11681700] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-79, X-RAY CRYSTALLOGRAPHY (1.64 ANGSTROMS).
[4]	"Studies of the ferredoxin from Thermus thermophilus." Hille R., Yoshida T., Tarr G.E., Williams C.H. Jr., Ludwig M.I., Fee J.A., Kent T.A., Huynh B.H., Munck E. J. Biol. Chem. 258:13008-13013(1983) [PubMed: 6313685] [Abstract] Cited for: AMINO-ACID COMPOSITION. Strain: ATCC 696.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AP008226 Genomic DNA. Translation: BAD70000.1.

PIR

FETWT. A00216.

RefSeq

YP_143443.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H98	X-ray	1.64	A	2-79	[»]

ModBase

Search...

Genome annotation databases

GeneID

3169609.

GenomeReviews

Gene locus TTHA0177 in contig AP008226_GR.

KEGG

ttj:TTHA0177.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P03942.

OMA

P03942. RACIEEC.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA0177-MON.

Family and domain databases

InterPro

IPR017896. 4Fe4S_Fe-S-bd.
IPR001450. 4Fe4S_Fe_S_bd_subgr.
IPR017900. 4Fe4S_Fe_S_CS.
IPR000813. 7Fe_ferredoxin.
[Graphical view]

Pfam

PF00037. Fer4. 1 hit.
[Graphical view]

PRINTS

PR00354. 7FE8SFRDOXIN.

PROSITE

PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FER_THET8

Accession

Primary (citable) accession number: P03942
Secondary accession number(s): Q5SLW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)