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P03940 (CP21A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 21-hydroxylase
EC=1.14.99.10
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene names
Name:Cyp21
Synonyms:Cyp21a-1, Cyp21a1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activity

A C21 steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Domain

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 487487Steroid 21-hydroxylase
PRO_0000051977

Regions

Region334 – 35017Steroid-binding By similarity

Sites

Metal binding4201Iron (heme axial ligand)

Experimental info

Sequence conflict31 – 322PP → LR in AAA37114. Ref.1
Sequence conflict661M → L in AAA37114. Ref.1
Sequence conflict2181L → F in BAA31153. Ref.3
Sequence conflict2381Q → R in BAA31153. Ref.3
Sequence conflict2381Q → R in AAC05278. Ref.4
Sequence conflict2411D → E in AAA37114. Ref.1
Sequence conflict3141E → A in AAA37114. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P03940 [UniParc].

Last modified April 26, 2005. Version 3.
Checksum: CAD32B606004449F

FASTA48755,328
        10         20         30         40         50         60 
MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL GLTQKLGPIY 

        70         80         90        100        110        120 
RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM DLDLSLGDYS LMWKAHKKLS 

       130        140        150        160        170        180 
RSALMLGMRD SMEPLIEQLT QEFCERMRAQ AGTPVAIHKE FSFLTCSIIS CLTFGDKDST 

       190        200        210        220        230        240 
LVQTLHDCVQ DLLQAWNHWS IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK 

       250        260        270        280        290        300 
DSLVAGQWKD MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA 

       310        320        330        340        350        360 
FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV VPLALPHRAT 

       370        380        390        400        410        420 
RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR FLEPGKNPRT PSFGCGARVC 

       430        440        450        460        470        480 
LGEPLARLEL FVVLARLLQA FTLLPPPDGT LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP 


QDQGERP

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence analysis of murine 21-hydroxylase genes: mutations affecting gene expression."
Chaplin D.D., Galbraith L.J., Seidman J.G., White P.C., Parker K.L.
Proc. Natl. Acad. Sci. U.S.A. 83:9601-9605(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The murine Slp gene: additional evidence that sex-limited protein has no biological function."
Zepf N.E., Ogata R.T.
J. Immunol. 147:2756-2763(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: B10.WR.
[3]"Mus musculus 5' truncated pseudogene of tenascin-X, steroid 21-hydroxylase (Cyp21), and sex-limited protein (Slp) genes, partial cds and complete sequences."
Matsumoto K., Ikuta T.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C57BL/6 X CBA.
[4]"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 129.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Liver mRNA probes disclose two cytochrome P-450 genes duplicated in tandem with the complement C4 loci of the mouse H-2S region."
Amor M., Tosi M., Duponchel C., Steinmetz M., Meo T.
Proc. Natl. Acad. Sci. U.S.A. 82:4453-4457(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-487.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M15009 Genomic DNA. Translation: AAA37114.1.
M64933 Genomic DNA. Translation: AAA40118.1.
AB015623 Genomic DNA. Translation: BAA31153.1.
AF049850 Genomic DNA. Translation: AAC05278.1.
BC127167 mRNA. Translation: AAI27168.1.
PIRA26660.
S54785.
RefSeqNP_034125.2. NM_009995.2.
UniGeneMm.440407.

3D structure databases

ProteinModelPortalP03940.
SMRP03940. Positions 29-475.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000025223.

PTM databases

PhosphoSiteP03940.

Proteomic databases

PRIDEP03940.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025223; ENSMUSP00000025223; ENSMUSG00000024365.
GeneID13079.
KEGGmmu:13079.
UCSCuc008cdo.2. mouse.

Organism-specific databases

CTD13079.
MGIMGI:88591. Cyp21a1.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117317.
HOVERGENHBG106944.
InParanoidA0JP50.
KOK00513.
OrthoDBEOG7BCNBG.
PhylomeDBP03940.
TreeFamTF105095.

Gene expression databases

ArrayExpressP03940.
BgeeP03940.
GenevestigatorP03940.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283032.
PROP03940.
SOURCESearch...

Entry information

Entry nameCP21A_MOUSE
AccessionPrimary (citable) accession number: P03940
Secondary accession number(s): A0JP50 expand/collapse secondary AC list , O88304, Q64510, Q9QX14
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: April 26, 2005
Last modified: April 16, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents