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P03940

- CP21A_MOUSE

UniProt

P03940 - CP21A_MOUSE

Protein

Steroid 21-hydroxylase

Gene

Cyp21

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 3 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

    Catalytic activityi

    A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi420 – 4201Iron (heme axial ligand)

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. steroid 21-monooxygenase activity Source: UniProtKB-EC
    4. steroid binding Source: UniProtKB-KW

    GO - Biological processi

    1. steroid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 21-hydroxylase (EC:1.14.99.10)
    Alternative name(s):
    21-OHase
    Cytochrome P-450c21
    Cytochrome P450 21
    Cytochrome P450 XXI
    Cytochrome P450-C21
    Gene namesi
    Name:Cyp21
    Synonyms:Cyp21a-1, Cyp21a1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:88591. Cyp21a1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 487487Steroid 21-hydroxylasePRO_0000051977Add
    BLAST

    Proteomic databases

    MaxQBiP03940.
    PRIDEiP03940.

    PTM databases

    PhosphoSiteiP03940.

    Expressioni

    Gene expression databases

    ArrayExpressiP03940.
    BgeeiP03940.
    GenevestigatoriP03940.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000025223.

    Structurei

    3D structure databases

    ProteinModelPortaliP03940.
    SMRiP03940. Positions 29-475.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni334 – 35017Steroid-bindingBy similarityAdd
    BLAST

    Domaini

    The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117317.
    HOVERGENiHBG106944.
    InParanoidiA0JP50.
    KOiK00513.
    OrthoDBiEOG7BCNBG.
    PhylomeDBiP03940.
    TreeFamiTF105095.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03940-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL    50
    GLTQKLGPIY RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM 100
    DLDLSLGDYS LMWKAHKKLS RSALMLGMRD SMEPLIEQLT QEFCERMRAQ 150
    AGTPVAIHKE FSFLTCSIIS CLTFGDKDST LVQTLHDCVQ DLLQAWNHWS 200
    IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK DSLVAGQWKD 250
    MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA 300
    FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV 350
    VPLALPHRAT RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR 400
    FLEPGKNPRT PSFGCGARVC LGEPLARLEL FVVLARLLQA FTLLPPPDGT 450
    LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP QDQGERP 487
    Length:487
    Mass (Da):55,328
    Last modified:April 26, 2005 - v3
    Checksum:iCAD32B606004449F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti31 – 322PP → LR in AAA37114. (PubMed:3491986)Curated
    Sequence conflicti66 – 661M → L in AAA37114. (PubMed:3491986)Curated
    Sequence conflicti218 – 2181L → F in BAA31153. 1 PublicationCurated
    Sequence conflicti238 – 2381Q → R in BAA31153. 1 PublicationCurated
    Sequence conflicti238 – 2381Q → R in AAC05278. (PubMed:14656967)Curated
    Sequence conflicti241 – 2411D → E in AAA37114. (PubMed:3491986)Curated
    Sequence conflicti314 – 3141E → A in AAA37114. (PubMed:3491986)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15009 Genomic DNA. Translation: AAA37114.1.
    M64933 Genomic DNA. Translation: AAA40118.1.
    AB015623 Genomic DNA. Translation: BAA31153.1.
    AF049850 Genomic DNA. Translation: AAC05278.1.
    BC127167 mRNA. Translation: AAI27168.1.
    CCDSiCCDS28658.1.
    PIRiA26660.
    S54785.
    RefSeqiNP_034125.2. NM_009995.2.
    UniGeneiMm.440407.

    Genome annotation databases

    EnsembliENSMUST00000025223; ENSMUSP00000025223; ENSMUSG00000024365.
    GeneIDi13079.
    KEGGimmu:13079.
    UCSCiuc008cdo.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15009 Genomic DNA. Translation: AAA37114.1 .
    M64933 Genomic DNA. Translation: AAA40118.1 .
    AB015623 Genomic DNA. Translation: BAA31153.1 .
    AF049850 Genomic DNA. Translation: AAC05278.1 .
    BC127167 mRNA. Translation: AAI27168.1 .
    CCDSi CCDS28658.1.
    PIRi A26660.
    S54785.
    RefSeqi NP_034125.2. NM_009995.2.
    UniGenei Mm.440407.

    3D structure databases

    ProteinModelPortali P03940.
    SMRi P03940. Positions 29-475.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000025223.

    PTM databases

    PhosphoSitei P03940.

    Proteomic databases

    MaxQBi P03940.
    PRIDEi P03940.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025223 ; ENSMUSP00000025223 ; ENSMUSG00000024365 .
    GeneIDi 13079.
    KEGGi mmu:13079.
    UCSCi uc008cdo.2. mouse.

    Organism-specific databases

    CTDi 13079.
    MGIi MGI:88591. Cyp21a1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117317.
    HOVERGENi HBG106944.
    InParanoidi A0JP50.
    KOi K00513.
    OrthoDBi EOG7BCNBG.
    PhylomeDBi P03940.
    TreeFami TF105095.

    Miscellaneous databases

    NextBioi 283032.
    PROi P03940.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P03940.
    Bgeei P03940.
    Genevestigatori P03940.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence analysis of murine 21-hydroxylase genes: mutations affecting gene expression."
      Chaplin D.D., Galbraith L.J., Seidman J.G., White P.C., Parker K.L.
      Proc. Natl. Acad. Sci. U.S.A. 83:9601-9605(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The murine Slp gene: additional evidence that sex-limited protein has no biological function."
      Zepf N.E., Ogata R.T.
      J. Immunol. 147:2756-2763(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: B10.WR.
    3. "Mus musculus 5' truncated pseudogene of tenascin-X, steroid 21-hydroxylase (Cyp21), and sex-limited protein (Slp) genes, partial cds and complete sequences."
      Matsumoto K., Ikuta T.
      Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C57BL/6 X CBA.
    4. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
      Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
      Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 129.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Liver mRNA probes disclose two cytochrome P-450 genes duplicated in tandem with the complement C4 loci of the mouse H-2S region."
      Amor M., Tosi M., Duponchel C., Steinmetz M., Meo T.
      Proc. Natl. Acad. Sci. U.S.A. 82:4453-4457(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-487.

    Entry informationi

    Entry nameiCP21A_MOUSE
    AccessioniPrimary (citable) accession number: P03940
    Secondary accession number(s): A0JP50
    , O88304, Q64510, Q9QX14
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 1986
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 127 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3