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Protein

Steroid 21-hydroxylase

Gene

Cyp21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.By similarity

Catalytic activityi

A C(21) steroid + [reduced NADPH--hemoprotein reductase] + O2 = a 21-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi420Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.14.16By similarity)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:Cyp21
Synonyms:Cyp21a-1, Cyp21a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88591. Cyp21a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519771 – 487Steroid 21-hydroxylaseAdd BLAST487

Proteomic databases

MaxQBiP03940.
PaxDbiP03940.
PRIDEiP03940.

PTM databases

iPTMnetiP03940.
PhosphoSitePlusiP03940.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024365.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025223.

Structurei

3D structure databases

ProteinModelPortaliP03940.
SMRiP03940.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni334 – 350Steroid-bindingBy similarityAdd BLAST17

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOVERGENiHBG106944.
InParanoidiP03940.
KOiK00513.
PhylomeDBiP03940.
TreeFamiTF105095.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL
60 70 80 90 100
GLTQKLGPIY RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM
110 120 130 140 150
DLDLSLGDYS LMWKAHKKLS RSALMLGMRD SMEPLIEQLT QEFCERMRAQ
160 170 180 190 200
AGTPVAIHKE FSFLTCSIIS CLTFGDKDST LVQTLHDCVQ DLLQAWNHWS
210 220 230 240 250
IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK DSLVAGQWKD
260 270 280 290 300
MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA
310 320 330 340 350
FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV
360 370 380 390 400
VPLALPHRAT RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR
410 420 430 440 450
FLEPGKNPRT PSFGCGARVC LGEPLARLEL FVVLARLLQA FTLLPPPDGT
460 470 480
LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP QDQGERP
Length:487
Mass (Da):55,328
Last modified:April 26, 2005 - v3
Checksum:iCAD32B606004449F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31 – 32PP → LR in AAA37114 (PubMed:3491986).Curated2
Sequence conflicti66M → L in AAA37114 (PubMed:3491986).Curated1
Sequence conflicti218L → F in BAA31153 (Ref. 3) Curated1
Sequence conflicti238Q → R in BAA31153 (Ref. 3) Curated1
Sequence conflicti238Q → R in AAC05278 (PubMed:14656967).Curated1
Sequence conflicti241D → E in AAA37114 (PubMed:3491986).Curated1
Sequence conflicti314E → A in AAA37114 (PubMed:3491986).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15009 Genomic DNA. Translation: AAA37114.1.
M64933 Genomic DNA. Translation: AAA40118.1.
AB015623 Genomic DNA. Translation: BAA31153.1.
AF049850 Genomic DNA. Translation: AAC05278.1.
BC127167 mRNA. Translation: AAI27168.1.
CCDSiCCDS28658.1.
PIRiA26660.
S54785.
RefSeqiNP_034125.2. NM_009995.2.
UniGeneiMm.440407.

Genome annotation databases

GeneIDi13079.
KEGGimmu:13079.
UCSCiuc008cdo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15009 Genomic DNA. Translation: AAA37114.1.
M64933 Genomic DNA. Translation: AAA40118.1.
AB015623 Genomic DNA. Translation: BAA31153.1.
AF049850 Genomic DNA. Translation: AAC05278.1.
BC127167 mRNA. Translation: AAI27168.1.
CCDSiCCDS28658.1.
PIRiA26660.
S54785.
RefSeqiNP_034125.2. NM_009995.2.
UniGeneiMm.440407.

3D structure databases

ProteinModelPortaliP03940.
SMRiP03940.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025223.

PTM databases

iPTMnetiP03940.
PhosphoSitePlusiP03940.

Proteomic databases

MaxQBiP03940.
PaxDbiP03940.
PRIDEiP03940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13079.
KEGGimmu:13079.
UCSCiuc008cdo.2. mouse.

Organism-specific databases

CTDi13079.
MGIiMGI:88591. Cyp21a1.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOVERGENiHBG106944.
InParanoidiP03940.
KOiK00513.
PhylomeDBiP03940.
TreeFamiTF105095.

Miscellaneous databases

PROiP03940.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024365.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP21A_MOUSE
AccessioniPrimary (citable) accession number: P03940
Secondary accession number(s): A0JP50
, O88304, Q64510, Q9QX14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: April 26, 2005
Last modified: November 2, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.