UniProtKB - P03940 (CP21A_MOUSE)
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Protein
Steroid 21-hydroxylase
Gene
Cyp21
Organism
Mus musculus (Mouse)
Status
Functioni
Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.By similarity
Catalytic activityi
A C(21) steroid + [reduced NADPH--hemoprotein reductase] + O2 = a 21-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity
Cofactori
hemeBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 420 | Iron (heme axial ligand) | 1 |
GO - Molecular functioni
- heme binding Source: MGI
- iron ion binding Source: InterPro
- steroid 21-monooxygenase activity Source: MGI
- steroid binding Source: UniProtKB-KW
- steroid hydroxylase activity Source: UniProtKB
GO - Biological processi
- steroid biosynthetic process Source: MGI
- steroid metabolic process Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Steroidogenesis |
Ligand | Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Steroid 21-hydroxylase (EC:1.14.14.16By similarity)Alternative name(s): 21-OHase Cytochrome P-450c21 Cytochrome P450 21 Cytochrome P450 XXI Cytochrome P450-C21 |
Gene namesi | Name:Cyp21 Synonyms:Cyp21a-1, Cyp21a1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88591 Cyp21a1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051977 | 1 – 487 | Steroid 21-hydroxylaseAdd BLAST | 487 |
Proteomic databases
MaxQBi | P03940 |
PaxDbi | P03940 |
PRIDEi | P03940 |
PTM databases
iPTMneti | P03940 |
PhosphoSitePlusi | P03940 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 334 – 350 | Steroid-bindingBy similarityAdd BLAST | 17 |
Domaini
The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
eggNOGi | KOG0156 Eukaryota COG2124 LUCA |
HOVERGENi | HBG106944 |
InParanoidi | P03940 |
KOi | K00513 |
PhylomeDBi | P03940 |
TreeFami | TF105095 |
Family and domain databases
Gene3Di | 1.10.630.101 hit |
InterProi | View protein in InterPro IPR001128 Cyt_P450 IPR017972 Cyt_P450_CS IPR002401 Cyt_P450_E_grp-I IPR036396 Cyt_P450_sf |
Pfami | View protein in Pfam PF00067 p450, 1 hit |
PRINTSi | PR00463 EP450I PR00385 P450 |
SUPFAMi | SSF48264 SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086 CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
P03940-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL
60 70 80 90 100
GLTQKLGPIY RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM
110 120 130 140 150
DLDLSLGDYS LMWKAHKKLS RSALMLGMRD SMEPLIEQLT QEFCERMRAQ
160 170 180 190 200
AGTPVAIHKE FSFLTCSIIS CLTFGDKDST LVQTLHDCVQ DLLQAWNHWS
210 220 230 240 250
IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK DSLVAGQWKD
260 270 280 290 300
MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA
310 320 330 340 350
FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV
360 370 380 390 400
VPLALPHRAT RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR
410 420 430 440 450
FLEPGKNPRT PSFGCGARVC LGEPLARLEL FVVLARLLQA FTLLPPPDGT
460 470 480
LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP QDQGERP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 – 32 | PP → LR in AAA37114 (PubMed:3491986).Curated | 2 | |
Sequence conflicti | 66 | M → L in AAA37114 (PubMed:3491986).Curated | 1 | |
Sequence conflicti | 218 | L → F in BAA31153 (Ref. 3) Curated | 1 | |
Sequence conflicti | 238 | Q → R in BAA31153 (Ref. 3) Curated | 1 | |
Sequence conflicti | 238 | Q → R in AAC05278 (PubMed:14656967).Curated | 1 | |
Sequence conflicti | 241 | D → E in AAA37114 (PubMed:3491986).Curated | 1 | |
Sequence conflicti | 314 | E → A in AAA37114 (PubMed:3491986).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15009 Genomic DNA Translation: AAA37114.1 M64933 Genomic DNA Translation: AAA40118.1 AB015623 Genomic DNA Translation: BAA31153.1 AF049850 Genomic DNA Translation: AAC05278.1 BC127167 mRNA Translation: AAI27168.1 |
CCDSi | CCDS28658.1 |
PIRi | A26660 S54785 |
RefSeqi | NP_034125.2, NM_009995.2 |
UniGenei | Mm.440407 |
Genome annotation databases
GeneIDi | 13079 |
KEGGi | mmu:13079 |
UCSCi | uc008cdo.2 mouse |
Similar proteinsi
Entry informationi
Entry namei | CP21A_MOUSE | |
Accessioni | P03940Primary (citable) accession number: P03940 Secondary accession number(s): A0JP50 Q9QX14 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 1986 |
Last sequence update: | April 26, 2005 | |
Last modified: | April 25, 2018 | |
This is version 151 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |