Reviewed, UniProtKB/Swiss-Prot P03940 (CP21A_MOUSE)

Names and origin

Protein names

Recommended name:
    Steroid 21-hydroxylase
    EC=1.14.99.10
Alternative name(s):
    Cytochrome P450 21
    Cytochrome P450 XXI
    21-OHase
    Cytochrome P450-C21
    Cytochrome P-450c21

Gene names

Name:	Cyp21
Synonyms:	Cyp21a-1, Cyp21a1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.
Catalytic activity	A steroid + AH2 + O2 = a 21-hydroxysteroid + A + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Domain	The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Lipid-binding Metal-binding Steroid-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 21-monooxygenase activity Inferred from electronic annotation. Source: EC steroid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Steroid 21-hydroxylase		PRO_0000051977
Regions
	Region	334 – 350	17	Steroid-binding By similarity
Sites
	Metal binding	420	1	Iron (heme axial ligand)
Experimental info
	Sequence conflict	31 – 32	2	PP → LR in AAA37114. Ref.1
	Sequence conflict	66	1	M → L in AAA37114. Ref.1
	Sequence conflict	218	1	L → F in BAA31153. Ref.3
	Sequence conflict	238	1	Q → R Ref.3
	Sequence conflict	238	1	Q → R Ref.4
	Sequence conflict	241	1	D → E in AAA37114. Ref.1
	Sequence conflict	314	1	E → A in AAA37114. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P03940-1 [UniParc]. Last modified April 26, 2005. Version 3. Checksum: CAD32B606004449F	FASTA	487	55,328
	10 20 30 40 50 60 MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL GLTQKLGPIY 70 80 90 100 110 120 RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM DLDLSLGDYS LMWKAHKKLS 130 140 150 160 170 180 RSALMLGMRD SMEPLIEQLT QEFCERMRAQ AGTPVAIHKE FSFLTCSIIS CLTFGDKDST 190 200 210 220 230 240 LVQTLHDCVQ DLLQAWNHWS IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK 250 260 270 280 290 300 DSLVAGQWKD MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA 310 320 330 340 350 360 FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV VPLALPHRAT 370 380 390 400 410 420 RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR FLEPGKNPRT PSFGCGARVC 430 440 450 460 470 480 LGEPLARLEL FVVLARLLQA FTLLPPPDGT LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP QDQGERP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis of murine 21-hydroxylase genes: mutations affecting gene expression." Chaplin D.D., Galbraith L.J., Seidman J.G., White P.C., Parker K.L. Proc. Natl. Acad. Sci. U.S.A. 83:9601-9605(1986) [PubMed: 3491986] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The murine Slp gene: additional evidence that sex-limited protein has no biological function." Zepf N.E., Ogata R.T. J. Immunol. 147:2756-2763(1991) [PubMed: 1918990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B10.WR.
[3]	"Mus musculus 5' truncated pseudogene of tenascin-X, steroid 21-hydroxylase (Cyp21), and sex-limited protein (Slp) genes, partial cds and complete sequences." Matsumoto K., Ikuta T. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6 X CBA.
[4]	"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse." Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L. Genome Res. 13:2621-2636(2003) [PubMed: 14656967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Liver mRNA probes disclose two cytochrome P-450 genes duplicated in tandem with the complement C4 loci of the mouse H-2S region." Amor M., Tosi M., Duponchel C., Steinmetz M., Meo T. Proc. Natl. Acad. Sci. U.S.A. 82:4453-4457(1985) [PubMed: 3874401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-487.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15009 Genomic DNA. Translation: AAA37114.1. M64933 Genomic DNA. Translation: AAA40118.1. AB015623 Genomic DNA. Translation: BAA31153.1. AF049850 Genomic DNA. Translation: AAC05278.1. BC127167 mRNA. Translation: AAI27168.1.
IPI	IPI00116920.
PIR	A26660. S54785.
RefSeq	NP_034125.2.
UniGene	Mm.440407
3D structure databases
SMR	P03940. Positions 44-476.
ModBase	Search...
Protein-protein interaction databases
STRING	P03940.
Proteomic databases
PRIDE	P03940.
Genome annotation databases
Ensembl	ENSMUST00000025223; ENSMUSP00000025223; ENSMUSG00000024365; Mus musculus. [Genome view]
GeneID	13079.
KEGG	mmu:13079.
Organism-specific databases
CTD	13079.
MGI	MGI:88591. Cyp21a1.
Phylogenomic databases
HOVERGEN	P03940.
InParanoid	P03940.
Enzyme and pathway databases
BRENDA	1.14.99.10. 244.
Gene expression databases
ArrayExpress	P03940.
Bgee	P03940.
Genevestigator	P03940.
GermOnline	ENSMUSG00000024365. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CP21A_MOUSE

Accession

Primary (citable) accession number: P03940
Secondary accession number(s): A0JP50 , O88304, Q64510, Q9QX14

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
Last sequence update:	April 26, 2005
Last modified:	January 19, 2010
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)