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Protein

Steroid 21-hydroxylase

Gene

Cyp21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activityi

A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi420 – 4201Iron (heme axial ligand)

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. steroid 21-monooxygenase activity Source: UniProtKB-EC
  4. steroid binding Source: UniProtKB-KW

GO - Biological processi

  1. steroid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Enzyme and pathway databases

ReactomeiREACT_281686. Endogenous sterols.
REACT_295803. Mineralocorticoid biosynthesis.
REACT_297756. Glucocorticoid biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.99.10)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:Cyp21
Synonyms:Cyp21a-1, Cyp21a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:88591. Cyp21a1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487Steroid 21-hydroxylasePRO_0000051977Add
BLAST

Proteomic databases

MaxQBiP03940.
PRIDEiP03940.

PTM databases

PhosphoSiteiP03940.

Expressioni

Gene expression databases

BgeeiP03940.
ExpressionAtlasiP03940. baseline and differential.
GenevestigatoriP03940.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025223.

Structurei

3D structure databases

ProteinModelPortaliP03940.
SMRiP03940. Positions 29-475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni334 – 35017Steroid-bindingBy similarityAdd
BLAST

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOVERGENiHBG106944.
InParanoidiP03940.
KOiK00513.
OrthoDBiEOG7BCNBG.
PhylomeDBiP03940.
TreeFamiTF105095.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL
60 70 80 90 100
GLTQKLGPIY RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM
110 120 130 140 150
DLDLSLGDYS LMWKAHKKLS RSALMLGMRD SMEPLIEQLT QEFCERMRAQ
160 170 180 190 200
AGTPVAIHKE FSFLTCSIIS CLTFGDKDST LVQTLHDCVQ DLLQAWNHWS
210 220 230 240 250
IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK DSLVAGQWKD
260 270 280 290 300
MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA
310 320 330 340 350
FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV
360 370 380 390 400
VPLALPHRAT RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR
410 420 430 440 450
FLEPGKNPRT PSFGCGARVC LGEPLARLEL FVVLARLLQA FTLLPPPDGT
460 470 480
LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP QDQGERP
Length:487
Mass (Da):55,328
Last modified:April 26, 2005 - v3
Checksum:iCAD32B606004449F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 322PP → LR in AAA37114 (PubMed:3491986).Curated
Sequence conflicti66 – 661M → L in AAA37114 (PubMed:3491986).Curated
Sequence conflicti218 – 2181L → F in BAA31153 (Ref. 3) Curated
Sequence conflicti238 – 2381Q → R in BAA31153 (Ref. 3) Curated
Sequence conflicti238 – 2381Q → R in AAC05278 (PubMed:14656967).Curated
Sequence conflicti241 – 2411D → E in AAA37114 (PubMed:3491986).Curated
Sequence conflicti314 – 3141E → A in AAA37114 (PubMed:3491986).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15009 Genomic DNA. Translation: AAA37114.1.
M64933 Genomic DNA. Translation: AAA40118.1.
AB015623 Genomic DNA. Translation: BAA31153.1.
AF049850 Genomic DNA. Translation: AAC05278.1.
BC127167 mRNA. Translation: AAI27168.1.
CCDSiCCDS28658.1.
PIRiA26660.
S54785.
RefSeqiNP_034125.2. NM_009995.2.
UniGeneiMm.440407.

Genome annotation databases

GeneIDi13079.
KEGGimmu:13079.
UCSCiuc008cdo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15009 Genomic DNA. Translation: AAA37114.1.
M64933 Genomic DNA. Translation: AAA40118.1.
AB015623 Genomic DNA. Translation: BAA31153.1.
AF049850 Genomic DNA. Translation: AAC05278.1.
BC127167 mRNA. Translation: AAI27168.1.
CCDSiCCDS28658.1.
PIRiA26660.
S54785.
RefSeqiNP_034125.2. NM_009995.2.
UniGeneiMm.440407.

3D structure databases

ProteinModelPortaliP03940.
SMRiP03940. Positions 29-475.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025223.

PTM databases

PhosphoSiteiP03940.

Proteomic databases

MaxQBiP03940.
PRIDEiP03940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13079.
KEGGimmu:13079.
UCSCiuc008cdo.2. mouse.

Organism-specific databases

CTDi13079.
MGIiMGI:88591. Cyp21a1.

Phylogenomic databases

eggNOGiCOG2124.
HOVERGENiHBG106944.
InParanoidiP03940.
KOiK00513.
OrthoDBiEOG7BCNBG.
PhylomeDBiP03940.
TreeFamiTF105095.

Enzyme and pathway databases

ReactomeiREACT_281686. Endogenous sterols.
REACT_295803. Mineralocorticoid biosynthesis.
REACT_297756. Glucocorticoid biosynthesis.

Miscellaneous databases

NextBioi283032.
PROiP03940.
SOURCEiSearch...

Gene expression databases

BgeeiP03940.
ExpressionAtlasiP03940. baseline and differential.
GenevestigatoriP03940.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis of murine 21-hydroxylase genes: mutations affecting gene expression."
    Chaplin D.D., Galbraith L.J., Seidman J.G., White P.C., Parker K.L.
    Proc. Natl. Acad. Sci. U.S.A. 83:9601-9605(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The murine Slp gene: additional evidence that sex-limited protein has no biological function."
    Zepf N.E., Ogata R.T.
    J. Immunol. 147:2756-2763(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B10.WR.
  3. "Mus musculus 5' truncated pseudogene of tenascin-X, steroid 21-hydroxylase (Cyp21), and sex-limited protein (Slp) genes, partial cds and complete sequences."
    Matsumoto K., Ikuta T.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6 X CBA.
  4. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
    Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
    Genome Res. 13:2621-2636(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Liver mRNA probes disclose two cytochrome P-450 genes duplicated in tandem with the complement C4 loci of the mouse H-2S region."
    Amor M., Tosi M., Duponchel C., Steinmetz M., Meo T.
    Proc. Natl. Acad. Sci. U.S.A. 82:4453-4457(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-487.

Entry informationi

Entry nameiCP21A_MOUSE
AccessioniPrimary (citable) accession number: P03940
Secondary accession number(s): A0JP50
, O88304, Q64510, Q9QX14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: April 26, 2005
Last modified: April 1, 2015
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.