Cyp21

Functionⁱ

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.By similarity

Catalytic activityⁱ

A C(21) steroid + [reduced NADPH--hemoprotein reductase] + O₂ = a 21-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H₂O.By similarity

Cofactorⁱ

hemeBy similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Metal bindingⁱ	420	Iron (heme axial ligand)		1

GO - Molecular functionⁱ

heme binding Source: MGI
iron ion binding Source: InterPro
steroid 21-monooxygenase activity Source: MGI
steroid binding Source: UniProtKB-KW
steroid hydroxylase activity Source: UniProtKB

Complete GO annotation...

GO - Biological processⁱ

steroid biosynthetic process Source: MGI
steroid metabolic process Source: UniProtKB

Complete GO annotation...

Keywordsⁱ

Molecular function	Monooxygenase, Oxidoreductase
Biological process	Steroidogenesis
Ligand	Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Steroid 21-hydroxylase (EC:1.14.14.16By similarity) Alternative name(s): 21-OHase Cytochrome P-450c21 Cytochrome P450 21 Cytochrome P450 XXI Cytochrome P450-C21
Gene namesⁱ	Name:Cyp21 Synonyms:Cyp21a-1, Cyp21a1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:88591. Cyp21a1.

Subcellular locationⁱ

GO - Cellular componentⁱ

endoplasmic reticulum membrane Source: UniProtKB-SubCell
organelle membrane Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000051977	1 – 487	Steroid 21-hydroxylaseAdd BLAST		487

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P03940.
PaxDbⁱ	P03940.
PRIDEⁱ	P03940.

PTM databases

iPTMnetⁱ	P03940.
PhosphoSitePlusⁱ	P03940.

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000024365.

Bgeeⁱ

ENSMUSG00000024365.

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000025223.

STRINGⁱ

10090.ENSMUSP00000025223.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P03940.
SMRⁱ	P03940.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	334 – 350	Steroid-bindingBy similarityAdd BLAST		17

Domainⁱ

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesⁱ

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0156. Eukaryota. COG2124. LUCA.
HOVERGENⁱ	HBG106944.
InParanoidⁱ	P03940.
KEGG Orthology (KO) More...KOⁱ	K00513.
PhylomeDBⁱ	P03940.
TreeFamⁱ	TF105095.

Family and domain databases

InterProⁱ	View protein in InterPro IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00067. p450. 1 hit.
PRINTSⁱ	PR00463. EP450I. PR00385. P450.
SUPFAMⁱ	SSF48264. SSF48264. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00086. CYTOCHROME_P450. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P03940-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL 
        60         70         80         90        100
GLTQKLGPIY RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM 
       110        120        130        140        150
DLDLSLGDYS LMWKAHKKLS RSALMLGMRD SMEPLIEQLT QEFCERMRAQ 
       160        170        180        190        200
AGTPVAIHKE FSFLTCSIIS CLTFGDKDST LVQTLHDCVQ DLLQAWNHWS 
       210        220        230        240        250
IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK DSLVAGQWKD 
       260        270        280        290        300
MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA 
       310        320        330        340        350
FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV 
       360        370        380        390        400
VPLALPHRAT RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR 
       410        420        430        440        450
FLEPGKNPRT PSFGCGARVC LGEPLARLEL FVVLARLLQA FTLLPPPDGT 
       460        470        480 
LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP QDQGERP

Length:487

Mass (Da):55,328

Last modified:April 26, 2005 - v3

Checksum:ⁱCAD32B606004449F

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	31 – 32	PP → LR in AAA37114 (PubMed:3491986).Curated	2
Sequence conflictⁱ	66	M → L in AAA37114 (PubMed:3491986).Curated	1
Sequence conflictⁱ	218	L → F in BAA31153 (Ref. 3) Curated	1
Sequence conflictⁱ	238	Q → R in BAA31153 (Ref. 3) Curated	1
Sequence conflictⁱ	238	Q → R in AAC05278 (PubMed:14656967).Curated	1
Sequence conflictⁱ	241	D → E in AAA37114 (PubMed:3491986).Curated	1
Sequence conflictⁱ	314	E → A in AAA37114 (PubMed:3491986).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M15009 Genomic DNA. Translation: AAA37114.1. M64933 Genomic DNA. Translation: AAA40118.1. AB015623 Genomic DNA. Translation: BAA31153.1. AF049850 Genomic DNA. Translation: AAC05278.1. BC127167 mRNA. Translation: AAI27168.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS28658.1.
PIRⁱ	A26660. S54785.
NCBI Reference Sequences More...RefSeqⁱ	NP_034125.2. NM_009995.2.
UniGeneⁱ	Mm.440407.

Genome annotation databases

GeneIDⁱ	13079.
KEGGⁱ	mmu:13079.
UCSC genome browser More...UCSCⁱ	uc008cdo.2. mouse.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M15009 Genomic DNA. Translation: AAA37114.1. M64933 Genomic DNA. Translation: AAA40118.1. AB015623 Genomic DNA. Translation: BAA31153.1. AF049850 Genomic DNA. Translation: AAC05278.1. BC127167 mRNA. Translation: AAI27168.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS28658.1.
PIRⁱ	A26660. S54785.
NCBI Reference Sequences More...RefSeqⁱ	NP_034125.2. NM_009995.2.
UniGeneⁱ	Mm.440407.

3D structure databases

ProteinModelPortalⁱ	P03940.
SMRⁱ	P03940.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000025223.

STRINGⁱ

10090.ENSMUSP00000025223.

PTM databases

iPTMnetⁱ	P03940.
PhosphoSitePlusⁱ	P03940.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P03940.
PaxDbⁱ	P03940.
PRIDEⁱ	P03940.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	13079.
KEGGⁱ	mmu:13079.
UCSC genome browser More...UCSCⁱ	uc008cdo.2. mouse.

Organism-specific databases

CTDⁱ	13079.
MGIⁱ	MGI:88591. Cyp21a1.

Phylogenomic databases

eggNOGⁱ	KOG0156. Eukaryota. COG2124. LUCA.
HOVERGENⁱ	HBG106944.
InParanoidⁱ	P03940.
KEGG Orthology (KO) More...KOⁱ	K00513.
PhylomeDBⁱ	P03940.
TreeFamⁱ	TF105095.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P03940.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000024365.

Bgeeⁱ

ENSMUSG00000024365.

Family and domain databases

InterProⁱ	View protein in InterPro IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00067. p450. 1 hit.
PRINTSⁱ	PR00463. EP450I. PR00385. P450.
SUPFAMⁱ	SSF48264. SSF48264. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00086. CYTOCHROME_P450. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CP21A_MOUSE
Accessionⁱ	P03940Primary (citable) accession number: P03940 Secondary accession number(s): A0JP50 , O88304, Q64510, Q9QX14
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
	Last sequence update:	April 26, 2005
	Last modified:	May 10, 2017
	This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P03940 (CP21A_MOUSE)

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Steroid 21-hydroxylase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ