Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P03940

- CP21A_MOUSE

UniProt

P03940 - CP21A_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Steroid 21-hydroxylase

Gene

Cyp21

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activityi

A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

Cofactori

Heme group.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi420 – 4201Iron (heme axial ligand)

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. steroid 21-monooxygenase activity Source: UniProtKB-EC
  4. steroid binding Source: UniProtKB-KW

GO - Biological processi

  1. steroid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.99.10)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:Cyp21
Synonyms:Cyp21a-1, Cyp21a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:88591. Cyp21a1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487Steroid 21-hydroxylasePRO_0000051977Add
BLAST

Proteomic databases

MaxQBiP03940.
PRIDEiP03940.

PTM databases

PhosphoSiteiP03940.

Expressioni

Gene expression databases

BgeeiP03940.
ExpressionAtlasiP03940. baseline and differential.
GenevestigatoriP03940.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025223.

Structurei

3D structure databases

ProteinModelPortaliP03940.
SMRiP03940. Positions 29-475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni334 – 35017Steroid-bindingBy similarityAdd
BLAST

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118992.
HOVERGENiHBG106944.
InParanoidiP03940.
KOiK00513.
OrthoDBiEOG7BCNBG.
PhylomeDBiP03940.
TreeFamiTF105095.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03940-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL
60 70 80 90 100
GLTQKLGPIY RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM
110 120 130 140 150
DLDLSLGDYS LMWKAHKKLS RSALMLGMRD SMEPLIEQLT QEFCERMRAQ
160 170 180 190 200
AGTPVAIHKE FSFLTCSIIS CLTFGDKDST LVQTLHDCVQ DLLQAWNHWS
210 220 230 240 250
IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK DSLVAGQWKD
260 270 280 290 300
MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA
310 320 330 340 350
FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV
360 370 380 390 400
VPLALPHRAT RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR
410 420 430 440 450
FLEPGKNPRT PSFGCGARVC LGEPLARLEL FVVLARLLQA FTLLPPPDGT
460 470 480
LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP QDQGERP
Length:487
Mass (Da):55,328
Last modified:April 26, 2005 - v3
Checksum:iCAD32B606004449F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 322PP → LR in AAA37114. (PubMed:3491986)Curated
Sequence conflicti66 – 661M → L in AAA37114. (PubMed:3491986)Curated
Sequence conflicti218 – 2181L → F in BAA31153. 1 PublicationCurated
Sequence conflicti238 – 2381Q → R in BAA31153. 1 PublicationCurated
Sequence conflicti238 – 2381Q → R in AAC05278. (PubMed:14656967)Curated
Sequence conflicti241 – 2411D → E in AAA37114. (PubMed:3491986)Curated
Sequence conflicti314 – 3141E → A in AAA37114. (PubMed:3491986)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15009 Genomic DNA. Translation: AAA37114.1.
M64933 Genomic DNA. Translation: AAA40118.1.
AB015623 Genomic DNA. Translation: BAA31153.1.
AF049850 Genomic DNA. Translation: AAC05278.1.
BC127167 mRNA. Translation: AAI27168.1.
CCDSiCCDS28658.1.
PIRiA26660.
S54785.
RefSeqiNP_034125.2. NM_009995.2.
UniGeneiMm.440407.

Genome annotation databases

EnsembliENSMUST00000025223; ENSMUSP00000025223; ENSMUSG00000024365.
GeneIDi13079.
KEGGimmu:13079.
UCSCiuc008cdo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15009 Genomic DNA. Translation: AAA37114.1 .
M64933 Genomic DNA. Translation: AAA40118.1 .
AB015623 Genomic DNA. Translation: BAA31153.1 .
AF049850 Genomic DNA. Translation: AAC05278.1 .
BC127167 mRNA. Translation: AAI27168.1 .
CCDSi CCDS28658.1.
PIRi A26660.
S54785.
RefSeqi NP_034125.2. NM_009995.2.
UniGenei Mm.440407.

3D structure databases

ProteinModelPortali P03940.
SMRi P03940. Positions 29-475.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000025223.

PTM databases

PhosphoSitei P03940.

Proteomic databases

MaxQBi P03940.
PRIDEi P03940.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025223 ; ENSMUSP00000025223 ; ENSMUSG00000024365 .
GeneIDi 13079.
KEGGi mmu:13079.
UCSCi uc008cdo.2. mouse.

Organism-specific databases

CTDi 13079.
MGIi MGI:88591. Cyp21a1.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00760000118992.
HOVERGENi HBG106944.
InParanoidi P03940.
KOi K00513.
OrthoDBi EOG7BCNBG.
PhylomeDBi P03940.
TreeFami TF105095.

Miscellaneous databases

NextBioi 283032.
PROi P03940.
SOURCEi Search...

Gene expression databases

Bgeei P03940.
ExpressionAtlasi P03940. baseline and differential.
Genevestigatori P03940.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis of murine 21-hydroxylase genes: mutations affecting gene expression."
    Chaplin D.D., Galbraith L.J., Seidman J.G., White P.C., Parker K.L.
    Proc. Natl. Acad. Sci. U.S.A. 83:9601-9605(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The murine Slp gene: additional evidence that sex-limited protein has no biological function."
    Zepf N.E., Ogata R.T.
    J. Immunol. 147:2756-2763(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B10.WR.
  3. "Mus musculus 5' truncated pseudogene of tenascin-X, steroid 21-hydroxylase (Cyp21), and sex-limited protein (Slp) genes, partial cds and complete sequences."
    Matsumoto K., Ikuta T.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6 X CBA.
  4. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
    Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
    Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Liver mRNA probes disclose two cytochrome P-450 genes duplicated in tandem with the complement C4 loci of the mouse H-2S region."
    Amor M., Tosi M., Duponchel C., Steinmetz M., Meo T.
    Proc. Natl. Acad. Sci. U.S.A. 82:4453-4457(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-487.

Entry informationi

Entry nameiCP21A_MOUSE
AccessioniPrimary (citable) accession number: P03940
Secondary accession number(s): A0JP50
, O88304, Q64510, Q9QX14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: April 26, 2005
Last modified: October 29, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3