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Protein

NADH-ubiquinone oxidoreductase chain 5

Gene

MT-ND5

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase chain 5 (EC:1.6.5.3)
Alternative name(s):
NADH dehydrogenase subunit 5
Gene namesi
Name:MT-ND5
Synonyms:MTND5, NADH5, ND5
Encoded oniMitochondrion
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Mitochondrion

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Transmembranei43 – 63HelicalSequence analysisAdd BLAST21
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Transmembranei171 – 191HelicalSequence analysisAdd BLAST21
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
Transmembranei273 – 293HelicalSequence analysisAdd BLAST21
Transmembranei310 – 330HelicalSequence analysisAdd BLAST21
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Transmembranei413 – 433HelicalSequence analysisAdd BLAST21
Transmembranei457 – 477HelicalSequence analysisAdd BLAST21
Transmembranei482 – 502HelicalSequence analysisAdd BLAST21
Transmembranei582 – 602HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial respiratory chain complex I Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001180681 – 606NADH-ubiquinone oxidoreductase chain 5Add BLAST606

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-formylmethionine1 Publication1

Keywords - PTMi

Formylation

Proteomic databases

PaxDbiP03920.
PRIDEiP03920.

Interactioni

Protein-protein interaction databases

IntActiP03920. 1 interactor.
STRINGi9913.ENSBTAP00000053152.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35L2-605[»]
5LDWelectron microscopy4.27L1-606[»]
5LDXelectron microscopy5.60L1-606[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4668. Eukaryota.
COG1009. LUCA.
HOGENOMiHOG000100570.
HOVERGENiHBG108343.
InParanoidiP03920.

Family and domain databases

InterProiIPR010934. NADH_DH_su5_C.
IPR003945. NADHpl_OxRdtase_5.
IPR001750. ND/Mrp_mem.
IPR001516. Proton_antipo_N.
[Graphical view]
PfamiPF06455. NADH5_C. 1 hit.
PF00361. Proton_antipo_M. 1 hit.
PF00662. Proton_antipo_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01974. NDH_I_L. 1 hit.

Sequencei

Sequence statusi: Complete.

P03920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMFSSLSLV TLLLLTMPIM MMSFNTYKPS NYPLYVKTAI SYAFITSMIP
60 70 80 90 100
TMMFIHSGQE LIISNWHWLT IQTLKLSLSF KMDYFSMMFI PVALFVTWSI
110 120 130 140 150
MEFSMWYMYS DPNINKFFKY LLLFLITMLI LVTANNLFQL FIGWEGVGIM
160 170 180 190 200
SFLLIGWWYG RADANTAALQ AILYNRIGDI GFILAMAWFL TNLNTWDLQQ
210 220 230 240 250
IFMLNPSDSN MPLIGLALAA TGKSAQFGLH PWLPSAMEGP TPVSALLHSS
260 270 280 290 300
TMVVAGIFLL IRFYPLTENN KYIQSITLCL GAITTLFTAM CALTQNDIKK
310 320 330 340 350
IIAFSTSSQL GLMMVTIGIN QPYLAFLHIC THAFFKAMLF MCSGSIIHSL
360 370 380 390 400
NDEQDIRKMG GLFKAMPFTT TALIVGSLAL TGMPFLTGFY SKDLIIEAAN
410 420 430 440 450
TSYTNAWALL MTLIATSFTA IYSTRIIFFA LLGQPRFPTL VNINENNPLL
460 470 480 490 500
INSIKRLLIG SLFAGYIISN NIPPTTIPQM TMPYYLKTTA LIVTILGFIL
510 520 530 540 550
ALEISNMTKN LKYHYPSNAF KFSTLLGYFP TIMHRLAPYM NLSMSQKSAS
560 570 580 590 600
SLLDLIWLEA ILPKTISLAQ MKASTLVTNQ KGLIKLYFLS FLITILISMI

LFNFHE
Length:606
Mass (Da):68,286
Last modified:February 12, 2003 - v2
Checksum:i3DC4371F0328B068
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti401T → K in CAA24005 (PubMed:7120390).Curated1

Mass spectrometryi

Molecular mass is 68319.8 Da from positions 1 - 606. Determined by ESI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti90I → T in strain: F. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA. Translation: CAA24005.1.
AF490528 Genomic DNA. Translation: AAM08327.1.
AF490529 Genomic DNA. Translation: AAM08340.1.
AF493541 Genomic DNA. Translation: AAM12799.1.
AF493542 Genomic DNA. Translation: AAM12812.1.
PIRiA00450. QXBO5M.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA. Translation: CAA24005.1.
AF490528 Genomic DNA. Translation: AAM08327.1.
AF490529 Genomic DNA. Translation: AAM08340.1.
AF493541 Genomic DNA. Translation: AAM12799.1.
AF493542 Genomic DNA. Translation: AAM12812.1.
PIRiA00450. QXBO5M.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35L2-605[»]
5LDWelectron microscopy4.27L1-606[»]
5LDXelectron microscopy5.60L1-606[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03920. 1 interactor.
STRINGi9913.ENSBTAP00000053152.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP03920.
PRIDEiP03920.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG4668. Eukaryota.
COG1009. LUCA.
HOGENOMiHOG000100570.
HOVERGENiHBG108343.
InParanoidiP03920.

Family and domain databases

InterProiIPR010934. NADH_DH_su5_C.
IPR003945. NADHpl_OxRdtase_5.
IPR001750. ND/Mrp_mem.
IPR001516. Proton_antipo_N.
[Graphical view]
PfamiPF06455. NADH5_C. 1 hit.
PF00361. Proton_antipo_M. 1 hit.
PF00662. Proton_antipo_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01974. NDH_I_L. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNU5M_BOVIN
AccessioniPrimary (citable) accession number: P03920
Secondary accession number(s): Q8SEN2, Q8SFW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 12, 2003
Last modified: November 30, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.