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Protein

NADH-ubiquinone oxidoreductase chain 4

Gene

MT-ND4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase chain 4 (EC:1.6.5.3)
Alternative name(s):
NADH dehydrogenase subunit 4
Gene namesi
Name:MT-ND4
Synonyms:MTND4, NADH4, ND4
Encoded oniMitochondrion
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Mitochondrion

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei22 – 4221HelicalSequence analysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence analysisAdd
BLAST
Transmembranei94 – 11219HelicalSequence analysisAdd
BLAST
Transmembranei116 – 13823HelicalSequence analysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence analysisAdd
BLAST
Transmembranei196 – 21621HelicalSequence analysisAdd
BLAST
Transmembranei224 – 24421HelicalSequence analysisAdd
BLAST
Transmembranei256 – 27621HelicalSequence analysisAdd
BLAST
Transmembranei284 – 30320HelicalSequence analysisAdd
BLAST
Transmembranei308 – 33023HelicalSequence analysisAdd
BLAST
Transmembranei351 – 37121HelicalSequence analysisAdd
BLAST
Transmembranei393 – 41321HelicalSequence analysisAdd
BLAST
Transmembranei435 – 45521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial respiratory chain complex I Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459NADH-ubiquinone oxidoreductase chain 4PRO_0000117906Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-formylmethionine1 Publication

Keywords - PTMi

Formylation

Proteomic databases

PaxDbiP03910.
PRIDEiP03910.

Expressioni

Gene expression databases

ExpressionAtlasiP03910. baseline.

Interactioni

Protein-protein interaction databases

IntActiP03910. 1 interaction.
STRINGi9913.ENSBTAP00000053150.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4845. Eukaryota.
COG1008. LUCA.
HOVERGENiHBG093667.
InParanoidiP03910.
KOiK03881.
OMAiIQTPWSY.
OrthoDBiEOG7DJSMN.
TreeFamiTF343520.

Family and domain databases

InterProiIPR000260. NADH4_N.
IPR010227. NADH_Q_OxRdtase_chainM/4.
IPR003918. NADH_UbQ_OxRdtase.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF01059. Oxidored_q5_N. 1 hit.
PF00361. Proton_antipo_M. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.
TIGRFAMsiTIGR01972. NDH_I_M. 1 hit.

Sequencei

Sequence statusi: Complete.

P03910-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKYIIPTIM LMPLTWLSKN NMIWVNSTAH SLLISFTSLL LMNQFGDNSL
60 70 80 90 100
NFSLLFFSDS LSTPLLILTM WLLPLMLMAS QHHLSKENLT RKKLFITMLI
110 120 130 140 150
SLQLFLIMTF TAMELILFYI LFEATLVPTL IIITRWGNQT ERLNAGLYFL
160 170 180 190 200
FYTLAGSLPL LVALIYIQNT VGSLNFLMLQ YWVQPVHNSW SNVFMWLACM
210 220 230 240 250
MAFMVKMPLY GLHLWLPKAH VEAPIAGSMV LAAVLLKLGG YGMLRITLIL
260 270 280 290 300
NPMTDFMAYP FIMLSLWGMI MTSSICLRQT DLKSLIAYSS VSHMALVIVA
310 320 330 340 350
ILIQTPWSYM GATALMIAHG LTSSMLFCLA NSNYERIHSR TMILARGLQT
360 370 380 390 400
LLPLMATWWL LASLTNLALP PTINLIGELF VVMSTFSWSN ITIILMGVNM
410 420 430 440 450
VITALYSLYM LIMTQRGKYT YHINNISPSF TRENALMSLH ILPLLLLTLN

PKIILGPLY
Length:459
Mass (Da):52,099
Last modified:July 21, 1986 - v1
Checksum:iEE7061AA41977CB4
GO

Mass spectrometryi

Molecular mass is 52130.0 Da from positions 1 - 459. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA. Translation: CAA24009.1. Sequence problems.
AF490528 Genomic DNA. Translation: AAM08326.1. Sequence problems.
AF490529 Genomic DNA. Translation: AAM08339.1. Sequence problems.
AF493541 Genomic DNA. Translation: AAM12798.1. Sequence problems.
AF493542 Genomic DNA. Translation: AAM12811.1. Sequence problems.
PIRiA00439. QXBO4M.
RefSeqiYP_209214.1. NC_006853.1.

Genome annotation databases

EnsembliENSBTAT00000060552; ENSBTAP00000053150; ENSBTAG00000043577.
GeneIDi3283886.
KEGGibta:3283886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA. Translation: CAA24009.1. Sequence problems.
AF490528 Genomic DNA. Translation: AAM08326.1. Sequence problems.
AF490529 Genomic DNA. Translation: AAM08339.1. Sequence problems.
AF493541 Genomic DNA. Translation: AAM12798.1. Sequence problems.
AF493542 Genomic DNA. Translation: AAM12811.1. Sequence problems.
PIRiA00439. QXBO4M.
RefSeqiYP_209214.1. NC_006853.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03910. 1 interaction.
STRINGi9913.ENSBTAP00000053150.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP03910.
PRIDEiP03910.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000060552; ENSBTAP00000053150; ENSBTAG00000043577.
GeneIDi3283886.
KEGGibta:3283886.

Organism-specific databases

CTDi4538.

Phylogenomic databases

eggNOGiKOG4845. Eukaryota.
COG1008. LUCA.
HOVERGENiHBG093667.
InParanoidiP03910.
KOiK03881.
OMAiIQTPWSY.
OrthoDBiEOG7DJSMN.
TreeFamiTF343520.

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Miscellaneous databases

NextBioi20810193.

Gene expression databases

ExpressionAtlasiP03910. baseline.

Family and domain databases

InterProiIPR000260. NADH4_N.
IPR010227. NADH_Q_OxRdtase_chainM/4.
IPR003918. NADH_UbQ_OxRdtase.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF01059. Oxidored_q5_N. 1 hit.
PF00361. Proton_antipo_M. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.
TIGRFAMsiTIGR01972. NDH_I_M. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome."
    Anderson S., de Bruijn M.H.L., Coulson A.R., Eperon I.C., Sanger F., Young I.G.
    J. Mol. Biol. 156:683-717(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HerefordImported.
    Tissue: Heart.
  2. "Bos taurus mitochondrial protein coding regions."
    Wettstein P.J.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 65, 66, D and F.
  3. "Definition of the mitochondrial proteome by measurement of molecular masses of membrane proteins."
    Carroll J., Fearnley I.M., Walker J.E.
    Proc. Natl. Acad. Sci. U.S.A. 103:16170-16175(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FORMYLATION AT MET-1, MASS SPECTROMETRY.

Entry informationi

Entry nameiNU4M_BOVIN
AccessioniPrimary (citable) accession number: P03910
Secondary accession number(s): Q8SE30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 17, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.