P03899 (NU3M_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-ubiquinone oxidoreductase chain 3
EC=1.6.5.3

Alternative name(s):
NADH dehydrogenase subunit 3

Gene names

Name:	Mtnd3
Synonyms:	mt-Nd3, Nd3

Encoded on

Mitochondrion

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex I Inferred from electronic annotation. Source: Compara mitochondrion Inferred from direct assay PubMed 18614015. Source: MGI
Molecular_function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 115	115	NADH-ubiquinone oxidoreductase chain 3		PRO_0000117765
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Transmembrane	55 – 75	21	Helical; Potential
	Transmembrane	86 – 106	21	Helical; Potential
Experimental info
	Sequence conflict	16	1	T → A in AAA19255. Ref.2
	Sequence conflict	33	1	Missing in AAB48651. Ref.1
	Sequence conflict	33	1	Missing in CAA24086. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P03899 [UniParc]. Last modified November 16, 2001. Version 3. Checksum: 6925F293601CB391	FASTA	115	13,219
	10 20 30 40 50 60 MNLYTVIFIN ILLSLTLILV AFWLPQMNLY SEKANPYECG FDPTSSARLP FSMKFFLVAI 70 80 90 100 110 TFLLFDLEIA LLLPLPWAIQ TIKTSTMMIM AFILVTILSL GLAYEWTQKG LEWTE

References

[1]	"Sequence and gene organization of mouse mitochondrial DNA." Bibb M.J., van Etten R.A., Wright C.T., Walberg M.W., Clayton D.A. Cell 26:167-180(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nonneutral evolution at the mitochondrial NADH dehydrogenase subunit 3 gene in mice." Nachman M.W., Boyer S.N., Aquadro C.F. Proc. Natl. Acad. Sci. U.S.A. 91:6364-6368(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Unique mutations in mitochondrial DNA of senescence-accelerated mouse (SAM) strains." Mizutani J., Chiba T., Tanaka M., Higuchi K., Mori M. J. Hered. 92:352-355(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AKR/J, C3H/He, SAMP1, SAMP8 and SAMR1. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01420 Genomic DNA. Translation: AAB48651.1. V00711 Genomic DNA. Translation: CAA24086.1. U09637 Genomic DNA. Translation: AAA19254.2. U09638 Genomic DNA. Translation: AAA19255.2. AB042432 Genomic DNA. Translation: BAA95623.1. AB042523 Genomic DNA. Translation: BAA95660.1. AB042524 Genomic DNA. Translation: BAA95647.1. AB042809 Genomic DNA. Translation: BAA95802.1. AB049357 Genomic DNA. Translation: BAB13808.1.
IPI	IPI00116170.
PIR	A55746. QXMS3M. B55746.
RefSeq	NP_904335.1. NC_005089.1. YP_220557.1. NC_006914.1.
3D structure databases
ProteinModelPortal	P03899.
SMR	P03899. Positions 3-114.
ModBase	Search...
PTM databases
PhosphoSite	P03899.
Proteomic databases
PaxDb	P03899.
PRIDE	P03899.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000082411; ENSMUSP00000080998; ENSMUSG00000064360.
GeneID	17718. 3338896.
KEGG	mmu:17718.
Organism-specific databases
CTD	4537.
MGI	MGI:102499. mt-Nd3.
Phylogenomic databases
eggNOG	COG0838.
HOGENOM	HOG000100119.
HOVERGEN	HBG017392.
InParanoid	P03899.
KO	K03880.
OrthoDB	EOG4NVZN1.
ProtClustDB	MTH00106.
Gene expression databases
Genevestigator	P03899.
Family and domain databases
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. PTHR11058. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	292338.
SOURCE	Search...

Entry information

Entry name

NU3M_MOUSE

Accession

Primary (citable) accession number: P03899
Secondary accession number(s): Q35039, Q35040, Q9MD73

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 16, 2001
Last modified:	April 3, 2013
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program