Reviewed, UniProtKB/Swiss-Prot P03898 (NU3M_BOVIN)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase chain 3
    EC=1.6.5.3
Alternative name(s):
    NADH dehydrogenase subunit 3

Gene names

Name:	MT-ND3
Synonyms:	MTND3, NADH3, ND3

Encoded on

Mitochondrion

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein Ref.3.
Sequence similarities	Belongs to the complex I subunit 3 family.
Mass spectrometry	Molecular mass is 13082.4 Da from positions 1 - 115. Determined by ESI. Ref.3

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Formylation
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 115	115	NADH-ubiquinone oxidoreductase chain 3		PRO_0000117716
Regions
	Transmembrane	3 – 23	21	Potential
	Transmembrane	55 – 75	21	Potential
	Transmembrane	84 – 104	21	Potential
Amino acid modifications
	Modified residue	1	1	N-formylmethionine

Sequences

	Sequence		Length	Mass (Da)
	P03898-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: A55C1B856B1C3515	FASTA	115	13,055
	10 20 30 40 50 60 MNLMLALLTN FTLATLLVII AFWLPQLNVY SEKTSPYECG FDPMGSARLP FSMKFFLVAI 70 80 90 100 110 TFLLFDLEIA LLLPLPWASQ TANLNTMLTM ALFLIILLAV SLAYEWTQKG LEWTE

References

[1]	"Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome." Anderson S., de Bruijn M.H.L., Coulson A.R., Eperon I.C., Sanger F., Young I.G. J. Mol. Biol. 156:683-717(1982) [PubMed: 7120390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Heart.
[2]	"Bos taurus mitochondrial protein coding regions." Wettstein P.J. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 65, 66, D and F.
[3]	"Definition of the mitochondrial proteome by measurement of molecular masses of membrane proteins." Carroll J., Fearnley I.M., Walker J.E. Proc. Natl. Acad. Sci. U.S.A. 103:16170-16175(2006) [PubMed: 17060615] [Abstract] Cited for: SUBCELLULAR LOCATION, FORMYLATION AT MET-1, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00654 Genomic DNA. Translation: CAA24008.1. Sequence problems. AF490528 Genomic DNA. Translation: AAM08324.1. Sequence problems. AF490529 Genomic DNA. Translation: AAM08337.1. Sequence problems. AF493541 Genomic DNA. Translation: AAM12796.1. Sequence problems. AF493542 Genomic DNA. Translation: AAM12809.1. Sequence problems.
IPI	IPI00716083.
PIR	QXBO3M. A00423.
RefSeq	YP_209212.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P03898.
Protein family/group databases
TCDB	3.D.1.6.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
Ensembl	ENSBTAT00000060547; ENSBTAP00000053159; ENSBTAG00000043568; Bos taurus. [Genome view]
GeneID	3283884.
KEGG	bta:3283884.
Organism-specific databases
CTD	3283884.
Phylogenomic databases
eggNOG	maNOG21081.
HOVERGEN	P03898.
InParanoid	P03898.
OrthoDB	EOG9NS5Z4.
PhylomeDB	P03898.
Enzyme and pathway databases
BRENDA	1.6.5.3. 251.
Family and domain databases
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NU3M_BOVIN

Accession

Primary (citable) accession number: P03898
Secondary accession number(s): Q8SEI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 19, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)