P03874 (CBP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome B pre-mRNA-processing protein 2

Gene names

Name:	CBP2
Ordered Locus Names:	YHL038C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Appears to be specifically required for the splicing of the terminal intron (bI5) of the cytochrome b pre-mRNA. Can also stimulates the splicing of the omega intron of the precursor of large ribosomal RNA.
Subcellular location	Mitochondrion.
Miscellaneous	Present with 1360 molecules/cell in log phase SD medium. Ref.5

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Mitochondrion
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Group I intron splicing Inferred from direct assay Ref.4. Source: SGD mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 630	630	Cytochrome B pre-mRNA-processing protein 2		PRO_0000089380
Experimental info
	Sequence conflict	490	1	G → S in AAA63570. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P03874 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 915413F4DD28827A	FASTA	630	73,861
	10 20 30 40 50 60 MVNWQTLFMV SLRRQGSSSR YRYKFNMENI THQVFPRCKQ AFKKTNLSYE YCDLEGVLYN 70 80 90 100 110 120 ISLTDLQKLL LRDINAPREH AFKIVRTDLT QKSSKKRIQH WERIAPMFDH PLSLYEKLFS 130 140 150 160 170 180 EMDEDFKPSF EWQQLIRVRC KDDKLKLQRV IWPKSIFSNF CRGIGVKKST YDRLLEQNNG 190 200 210 220 230 240 EVPMFVNPAN AKPLPLFQVS DEAMIGEFDG IGIFPYFVDK HREFFVTEVD KLKTKIASPL 250 260 270 280 290 300 CTLNERKRIE KANAGRILAN EEGKPFYLDA NSATTRIAGG NVVTLKQLLE RSVSHKTLWS 310 320 330 340 350 360 KQSNKDRTCP GDILRATILS NDFSIRQLRA EFCKNFILYN IFTILQRNKK SIRSFSGNDN 370 380 390 400 410 420 APSFQFSWNV WDSYIWKQYQ ETESMKIPTD QASLINYKTK YDSFLHDLQT YSALVISEMK 430 440 450 460 470 480 WNQFSIFQND ETTLSRFEHI TLILQTVLTK SKMIRIFQPN LYKFMQDDLR PTLMELVGFT 490 500 510 520 530 540 ESINATIEPG FANEQSLQSA NGLKKLANQL LYFEQEIYGE KFRVNRPIQL RPITLSANYK 550 560 570 580 590 600 IVILDKKNAI PEIFQTLLKF MTQITTYFVK DLSEVELHGH MHCIDKKMLD KSKFMYLYEE 610 620 630 KSNEEVKAAS PQKEKIVDNI IGLLSNDEEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Assembly of the mitochondrial membrane system. Characterization of a yeast nuclear gene involved in the processing of the cytochrome b pre-mRNA." McGraw P., Tzagoloff A. J. Biol. Chem. 258:9459-9468(1983) [PubMed: 6348045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The Cbp2 protein stimulates the splicing of the omega intron of yeast mitochondria." Shaw L.C., Lewin A.S. Nucleic Acids Res. 25:1597-1604(1997) [PubMed: 9092668] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K00138 Genomic DNA. Translation: AAA63570.1. U11583 Genomic DNA. Translation: AAB65050.1. BK006934 Genomic DNA. Translation: DAA06648.1.
PIR	BWBY. S48930.
RefSeq	NP_011825.1. NM_001179118.1.
3D structure databases
ProteinModelPortal	P03874.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6581N.
IntAct	P03874. 2 interactions.
MINT	MINT-680365.
STRING	P03874.
Proteomic databases
PeptideAtlas	P03874.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL038C; YHL038C; YHL038C.
GeneID	856347.
KEGG	sce:YHL038C.
NMPDR	fig|4932.3.peg.2961.
Organism-specific databases
CYGD	YHL038c.
SGD	S000001030. CBP2.
Phylogenomic databases
eggNOG	fuNOG14903.
OMA	MENITHQ.
OrthoDB	EOG4RFQ34.
Gene expression databases
ArrayExpress	P03874.
Genevestigator	P03874.
GermOnline	YHL038C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016424. Cyt_B_pre-mRNA-process_2. [Graphical view]
PIRSF	PIRSF004611. CBP2. 1 hit.
ProtoNet	Search...
Other
NextBio	981781.

Entry information

Entry name

CBP2_YEAST

Accession

Primary (citable) accession number: P03874
Secondary accession number(s): D3DKT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program