P03872 (REP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Partitioning protein REP2
Short name=R2

Alternative name(s):
Protein Charlie
Trans-acting factor C

Gene names

Name:	REP2
Ordered Locus Names:	R0040C

Encoded on

Plasmid 2-micron

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1. Ref.2 Ref.5 Ref.7 Ref.8
Subunit structure	Interacts with REP1. Ref.3 Ref.6
Subcellular location	Nucleus. Note: Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases. Ref.3 Ref.4 Ref.5
Miscellaneous	The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

Ontologies

Keywords
Biological process	Plasmid partition
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Plasmid Reference proteome
Gene Ontology (GO)
Biological_process	2-micrometer plasmid partitioning Inferred from mutant phenotype Ref.3. Source: SGD
Cellular_component	nucleus Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		5	EBI-2125362,EBI-2125362
REP1	P03871	7	EBI-2125362,EBI-14929

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Partitioning protein REP2		PRO_0000150898
Regions
	Region	1 – 57	57	Interaction with REP1
	Region	58 – 296	239	DNA-binding, and self-association
	Region	276 – 296	21	Nuclear localization
Amino acid modifications
	Modified residue	107	1	Phosphoserine Ref.9
	Modified residue	108	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P03872 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: FF54CBC6B90F5FAF	FASTA	296	33,196
	10 20 30 40 50 60 MDDIETAKNL TVKARTAYSV WDVCRLFIEM IAPDVDIDIE SKRKSDELLF PGYVIRPMES 70 80 90 100 110 120 LTTGRPYGLD SSAEDSSVSS DSSAEVILPA AKMVKERFDS IGNGMLSSQE ASQAAIDLML 130 140 150 160 170 180 QNNKLLDNRK QLYKSIAIII GRLPEKDKKR ATEMLMRKMD CTQLLVPPAP TEEDVMKLVS 190 200 210 220 230 240 VVTQLLTLVP PDRQAALIGD LFIPESLKDI FNSFNELAAE NRLQQKKSEL EGRTEVNHAN 250 260 270 280 290 TNEEVPSRRT RSRDTNARGA YKLQNTITEG PKAVPTKKRR VATRVRGRKS RNTSRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the yeast plasmid." Hartley J.L., Donelson J.E. Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A364A D5.
[2]	"Antagonistic controls regulate copy number of the yeast 2 micron plasmid." Murray J.A.H., Scarpa M., Rossi N., Cesareni G. EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"The 2 micrometer-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus." Ahn Y.-T., Wu X.-L., Biswal S., Velmurugan S., Volkert F.C., Jayaram M. J. Bacteriol. 179:7497-7506(1997) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH REP1.
[4]	"The 2 micrometer plasmid stability system: analyses of the interactions among plasmid- and host-encoded components." Velmurugan S., Ahn Y.-T., Yang X.-M., Wu X.-L., Jayaram M. Mol. Cell. Biol. 18:7466-7477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Partitioning of the 2-micrometer circle plasmid of Saccharomyces cerevisiae. Functional coordination with chromosome segregation and plasmid-encoded Rep protein distribution." Velmurugan S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M. J. Cell Biol. 149:553-566(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Functional domains of yeast plasmid-encoded Rep proteins." Sengupta A., Blomqvist K., Pickett A.J., Zhang Y., Chew J.S.K., Dobson M.J. J. Bacteriol. 183:2306-2315(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH REP1, DNA-BINDING.
[7]	"The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?" Mehta S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M., Velmurugan S. J. Cell Biol. 158:625-637(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-micrometer circle partitioning locus and promotes equal plasmid segregation." Hajra S., Ghosh S.K., Jayaram M. J. Cell Biol. 174:779-790(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107 AND SER-108, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01347 Genomic DNA. Translation: AAB59343.1.
PIR	PDBYC. A04504.
RefSeq	NP_040491.1. NC_001398.1.
3D structure databases
ProteinModelPortal	P03872.
ModBase	Search...
Protein-protein interaction databases
IntAct	P03872. 1 interaction.
STRING	4932.R0040C.
Proteomic databases
PaxDb	P03872.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
CYGD	R0040c.
SGD	S000029676. REP2.
Gene expression databases
Genevestigator	P03872.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

REP2_YEAST

Accession

Primary (citable) accession number: P03872

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program