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P03872

- REP2_YEAST

UniProt

P03872 - REP2_YEAST

Protein

Partitioning protein REP2

Gene

REP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1.4 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct

    GO - Biological processi

    1. 2-micrometer plasmid partitioning Source: SGD
    2. protein stabilization Source: SGD

    Keywords - Biological processi

    Plasmid partition

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Partitioning protein REP2
    Short name:
    R2
    Alternative name(s):
    Protein Charlie
    Trans-acting factor C
    Gene namesi
    Name:REP2
    Ordered Locus Names:R0040C
    Encoded oniPlasmid 2-micron0 Publication
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Plasmid 2-micron

    Organism-specific databases

    CYGDiR0040c.
    SGDiS000029676. REP2.

    Subcellular locationi

    Nucleus 3 Publications
    Note: Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases.

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 296296Partitioning protein REP2PRO_0000150898Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP03872.
    PaxDbiP03872.

    Expressioni

    Gene expression databases

    GenevestigatoriP03872.

    Interactioni

    Subunit structurei

    Interacts with REP1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself5EBI-2125362,EBI-2125362
    REP1P038717EBI-2125362,EBI-14929

    Protein-protein interaction databases

    IntActiP03872. 1 interaction.
    STRINGi4932.R0040C.

    Structurei

    3D structure databases

    ProteinModelPortaliP03872.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 5757Interaction with REP1Add
    BLAST
    Regioni58 – 296239DNA-binding, and self-associationAdd
    BLAST
    Regioni276 – 29621Nuclear localizationAdd
    BLAST

    Phylogenomic databases

    OrthoDBiEOG71VT45.

    Sequencei

    Sequence statusi: Complete.

    P03872-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDIETAKNL TVKARTAYSV WDVCRLFIEM IAPDVDIDIE SKRKSDELLF    50
    PGYVIRPMES LTTGRPYGLD SSAEDSSVSS DSSAEVILPA AKMVKERFDS 100
    IGNGMLSSQE ASQAAIDLML QNNKLLDNRK QLYKSIAIII GRLPEKDKKR 150
    ATEMLMRKMD CTQLLVPPAP TEEDVMKLVS VVTQLLTLVP PDRQAALIGD 200
    LFIPESLKDI FNSFNELAAE NRLQQKKSEL EGRTEVNHAN TNEEVPSRRT 250
    RSRDTNARGA YKLQNTITEG PKAVPTKKRR VATRVRGRKS RNTSRV 296
    Length:296
    Mass (Da):33,196
    Last modified:July 21, 1986 - v1
    Checksum:iFF54CBC6B90F5FAF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01347 Genomic DNA. Translation: AAB59343.1.
    PIRiA04504. PDBYC.
    RefSeqiNP_040491.1. NC_001398.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01347 Genomic DNA. Translation: AAB59343.1 .
    PIRi A04504. PDBYC.
    RefSeqi NP_040491.1. NC_001398.1.

    3D structure databases

    ProteinModelPortali P03872.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P03872. 1 interaction.
    STRINGi 4932.R0040C.

    Proteomic databases

    MaxQBi P03872.
    PaxDbi P03872.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    CYGDi R0040c.
    SGDi S000029676. REP2.

    Phylogenomic databases

    OrthoDBi EOG71VT45.

    Gene expression databases

    Genevestigatori P03872.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the yeast plasmid."
      Hartley J.L., Donelson J.E.
      Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A364A D5.
    2. "Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
      Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
      EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    3. "The 2 micrometer-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus."
      Ahn Y.-T., Wu X.-L., Biswal S., Velmurugan S., Volkert F.C., Jayaram M.
      J. Bacteriol. 179:7497-7506(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH REP1.
    4. "The 2 micrometer plasmid stability system: analyses of the interactions among plasmid- and host-encoded components."
      Velmurugan S., Ahn Y.-T., Yang X.-M., Wu X.-L., Jayaram M.
      Mol. Cell. Biol. 18:7466-7477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "Partitioning of the 2-micrometer circle plasmid of Saccharomyces cerevisiae. Functional coordination with chromosome segregation and plasmid-encoded Rep protein distribution."
      Velmurugan S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M.
      J. Cell Biol. 149:553-566(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. Cited for: INTERACTION WITH REP1, DNA-BINDING.
    7. "The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?"
      Mehta S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M., Velmurugan S.
      J. Cell Biol. 158:625-637(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-micrometer circle partitioning locus and promotes equal plasmid segregation."
      Hajra S., Ghosh S.K., Jayaram M.
      J. Cell Biol. 174:779-790(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiREP2_YEAST
    AccessioniPrimary (citable) accession number: P03872
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

    External Data

    Dasty 3