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P03872

- REP2_YEAST

UniProt

P03872 - REP2_YEAST

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Protein

Partitioning protein REP2

Gene

REP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1.4 Publications

GO - Molecular functioni

  1. identical protein binding Source: IntAct

GO - Biological processi

  1. 2-micrometer plasmid partitioning Source: SGD
  2. protein stabilization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Plasmid partition

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning protein REP2
Short name:
R2
Alternative name(s):
Protein Charlie
Trans-acting factor C
Gene namesi
Name:REP2
Ordered Locus Names:R0040C
Encoded oniPlasmid 2-micron0 Publication
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Plasmid 2-micron

Organism-specific databases

CYGDiR0040c.
SGDiS000029676. REP2.

Subcellular locationi

Nucleus 3 Publications
Note: Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases.

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Partitioning protein REP2PRO_0000150898Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP03872.
PaxDbiP03872.

Expressioni

Gene expression databases

GenevestigatoriP03872.

Interactioni

Subunit structurei

Interacts with REP1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-2125362,EBI-2125362
REP1P038717EBI-2125362,EBI-14929

Protein-protein interaction databases

IntActiP03872. 1 interaction.
STRINGi4932.R0040C.

Structurei

3D structure databases

ProteinModelPortaliP03872.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 5757Interaction with REP1Add
BLAST
Regioni58 – 296239DNA-binding, and self-associationAdd
BLAST
Regioni276 – 29621Nuclear localizationAdd
BLAST

Phylogenomic databases

OrthoDBiEOG71VT45.

Sequencei

Sequence statusi: Complete.

P03872-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDIETAKNL TVKARTAYSV WDVCRLFIEM IAPDVDIDIE SKRKSDELLF
60 70 80 90 100
PGYVIRPMES LTTGRPYGLD SSAEDSSVSS DSSAEVILPA AKMVKERFDS
110 120 130 140 150
IGNGMLSSQE ASQAAIDLML QNNKLLDNRK QLYKSIAIII GRLPEKDKKR
160 170 180 190 200
ATEMLMRKMD CTQLLVPPAP TEEDVMKLVS VVTQLLTLVP PDRQAALIGD
210 220 230 240 250
LFIPESLKDI FNSFNELAAE NRLQQKKSEL EGRTEVNHAN TNEEVPSRRT
260 270 280 290
RSRDTNARGA YKLQNTITEG PKAVPTKKRR VATRVRGRKS RNTSRV
Length:296
Mass (Da):33,196
Last modified:July 21, 1986 - v1
Checksum:iFF54CBC6B90F5FAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01347 Genomic DNA. Translation: AAB59343.1.
PIRiA04504. PDBYC.
RefSeqiNP_040491.1. NC_001398.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01347 Genomic DNA. Translation: AAB59343.1 .
PIRi A04504. PDBYC.
RefSeqi NP_040491.1. NC_001398.1.

3D structure databases

ProteinModelPortali P03872.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P03872. 1 interaction.
STRINGi 4932.R0040C.

Proteomic databases

MaxQBi P03872.
PaxDbi P03872.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

CYGDi R0040c.
SGDi S000029676. REP2.

Phylogenomic databases

OrthoDBi EOG71VT45.

Gene expression databases

Genevestigatori P03872.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the yeast plasmid."
    Hartley J.L., Donelson J.E.
    Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A364A D5.
  2. "Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
    Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
    EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "The 2 micrometer-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus."
    Ahn Y.-T., Wu X.-L., Biswal S., Velmurugan S., Volkert F.C., Jayaram M.
    J. Bacteriol. 179:7497-7506(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH REP1.
  4. "The 2 micrometer plasmid stability system: analyses of the interactions among plasmid- and host-encoded components."
    Velmurugan S., Ahn Y.-T., Yang X.-M., Wu X.-L., Jayaram M.
    Mol. Cell. Biol. 18:7466-7477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Partitioning of the 2-micrometer circle plasmid of Saccharomyces cerevisiae. Functional coordination with chromosome segregation and plasmid-encoded Rep protein distribution."
    Velmurugan S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M.
    J. Cell Biol. 149:553-566(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. Cited for: INTERACTION WITH REP1, DNA-BINDING.
  7. "The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?"
    Mehta S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M., Velmurugan S.
    J. Cell Biol. 158:625-637(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-micrometer circle partitioning locus and promotes equal plasmid segregation."
    Hajra S., Ghosh S.K., Jayaram M.
    J. Cell Biol. 174:779-790(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiREP2_YEAST
AccessioniPrimary (citable) accession number: P03872
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

External Data

Dasty 3