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P03872 (REP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Partitioning protein REP2

Short name=R2
Alternative name(s):
Protein Charlie
Trans-acting factor C
Gene names
Name:REP2
Ordered Locus Names:R0040C
Encoded onPlasmid 2-micron
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1. Ref.2 Ref.5 Ref.7 Ref.8

Subunit structure

Interacts with REP1. Ref.3 Ref.6

Subcellular location

Nucleus. Note: Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases. Ref.3 Ref.4 Ref.5

Miscellaneous

The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

Ontologies

Keywords
   Biological processPlasmid partition
   Cellular componentNucleus
   PTMAcetylation
   Technical termComplete proteome
Plasmid
Reference proteome
Gene Ontology (GO)
   Biological_process2-micrometer plasmid partitioning

Inferred from mutant phenotype Ref.3. Source: SGD

protein stabilization

Inferred from mutant phenotype PubMed 23555963. Source: SGD

   Cellular_componentnucleus

Inferred from direct assay Ref.3. Source: SGD

   Molecular_functionidentical protein binding

Inferred from physical interaction Ref.3. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself5EBI-2125362,EBI-2125362
REP1P038717EBI-2125362,EBI-14929

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296Partitioning protein REP2
PRO_0000150898

Regions

Region1 – 5757Interaction with REP1
Region58 – 296239DNA-binding, and self-association
Region276 – 29621Nuclear localization

Amino acid modifications

Modified residue11N-acetylmethionine Ref.10

Sequences

Sequence LengthMass (Da)Tools
P03872 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: FF54CBC6B90F5FAF

FASTA29633,196
        10         20         30         40         50         60 
MDDIETAKNL TVKARTAYSV WDVCRLFIEM IAPDVDIDIE SKRKSDELLF PGYVIRPMES 

        70         80         90        100        110        120 
LTTGRPYGLD SSAEDSSVSS DSSAEVILPA AKMVKERFDS IGNGMLSSQE ASQAAIDLML 

       130        140        150        160        170        180 
QNNKLLDNRK QLYKSIAIII GRLPEKDKKR ATEMLMRKMD CTQLLVPPAP TEEDVMKLVS 

       190        200        210        220        230        240 
VVTQLLTLVP PDRQAALIGD LFIPESLKDI FNSFNELAAE NRLQQKKSEL EGRTEVNHAN 

       250        260        270        280        290 
TNEEVPSRRT RSRDTNARGA YKLQNTITEG PKAVPTKKRR VATRVRGRKS RNTSRV 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the yeast plasmid."
Hartley J.L., Donelson J.E.
Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A364A D5.
[2]"Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"The 2 micrometer-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus."
Ahn Y.-T., Wu X.-L., Biswal S., Velmurugan S., Volkert F.C., Jayaram M.
J. Bacteriol. 179:7497-7506(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH REP1.
[4]"The 2 micrometer plasmid stability system: analyses of the interactions among plasmid- and host-encoded components."
Velmurugan S., Ahn Y.-T., Yang X.-M., Wu X.-L., Jayaram M.
Mol. Cell. Biol. 18:7466-7477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Partitioning of the 2-micrometer circle plasmid of Saccharomyces cerevisiae. Functional coordination with chromosome segregation and plasmid-encoded Rep protein distribution."
Velmurugan S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M.
J. Cell Biol. 149:553-566(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Functional domains of yeast plasmid-encoded Rep proteins."
Sengupta A., Blomqvist K., Pickett A.J., Zhang Y., Chew J.S.K., Dobson M.J.
J. Bacteriol. 183:2306-2315(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH REP1, DNA-BINDING.
[7]"The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?"
Mehta S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M., Velmurugan S.
J. Cell Biol. 158:625-637(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-micrometer circle partitioning locus and promotes equal plasmid segregation."
Hajra S., Ghosh S.K., Jayaram M.
J. Cell Biol. 174:779-790(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J01347 Genomic DNA. Translation: AAB59343.1.
PIRPDBYC. A04504.
RefSeqNP_040491.1. NC_001398.1.

3D structure databases

ProteinModelPortalP03872.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP03872. 1 interaction.
STRING4932.R0040C.

Proteomic databases

PaxDbP03872.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

CYGDR0040c.
SGDS000029676. REP2.

Phylogenomic databases

OrthoDBEOG71VT45.

Gene expression databases

GenevestigatorP03872.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameREP2_YEAST
AccessionPrimary (citable) accession number: P03872
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD