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P03871

- REP1_YEAST

UniProt

P03871 - REP1_YEAST

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Protein

Partitioning protein REP1

Gene

REP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1.5 Publications

GO - Molecular functioni

  1. identical protein binding Source: IntAct

GO - Biological processi

  1. 2-micrometer plasmid partitioning Source: SGD
  2. cellular protein localization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Plasmid partition

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning protein REP1
Short name:
R1
Alternative name(s):
Protein Baker
Trans-acting factor B
Gene namesi
Name:REP1
Ordered Locus Names:R0020C
Encoded oniPlasmid 2-micron0 Publication
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Plasmid 2-micron

Organism-specific databases

CYGDiR0020c.
SGDiS000029675. REP1.

Subcellular locationi

Nucleus 3 Publications
Note: Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases.

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi32 – 321T → K: Abolishes interaction with REP2 and STB. 1 Publication
Mutagenesisi43 – 431Y → A: Abolishes interaction with REP2, but not with STB. 1 Publication
Mutagenesisi50 – 501A → D: Abolishes interaction with REP2, but not with STB. 1 Publication
Mutagenesisi78 – 781V → K: Abolishes interaction with REP2, but not with STB. 1 Publication
Mutagenesisi93 – 931S → Y: Abolishes interaction with REP2, but not with STB. 1 Publication
Mutagenesisi154 – 1541L → S: Abolishes interaction with STB, but not with REP2. 1 Publication
Mutagenesisi200 – 2001E → A: Abolishes interaction with REP2, but not with STB. 1 Publication
Mutagenesisi276 – 2761A → P: Abolishes interaction with REP2, but not with STB. 1 Publication
Mutagenesisi297 – 2971K → Q: Abolishes interaction with STB, but not with REP2. 1 Publication
Mutagenesisi301 – 3011Y → L: Abolishes interaction with REP2, but not with STB. 1 Publication
Mutagenesisi308 – 3081I → Y: Abolishes interaction with REP2, but not with STB. 1 Publication
Mutagenesisi317 – 3171Y → I: Abolishes interaction with STB, but not with REP2. 1 Publication
Mutagenesisi330 – 3301S → Y: Abolishes interaction with STB, but not with REP2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Partitioning protein REP1PRO_0000150892Add
BLAST

Proteomic databases

MaxQBiP03871.
PaxDbiP03871.

Expressioni

Gene expression databases

GenevestigatoriP03871.

Interactioni

Subunit structurei

Interacts with REP2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-14929,EBI-14929
REP2P038727EBI-14929,EBI-2125362

Protein-protein interaction databases

DIPiDIP-7698N.
IntActiP03871. 7 interactions.
MINTiMINT-687041.
STRINGi4932.R0020C.

Structurei

3D structure databases

ProteinModelPortaliP03871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 129129Interaction with REP2 and self-associationAdd
BLAST
Regioni1 – 7676Interaction with REP2Add
BLAST
Regioni349 – 37325Nuclear localizationAdd
BLAST

Phylogenomic databases

eggNOGiNOG326131.
InParanoidiP03871.
OrthoDBiEOG7B31XF.

Family and domain databases

InterProiIPR008897. Yeast_TAF.
[Graphical view]
PfamiPF05797. Rep_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03871-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGERLLACI KQCIMQHFQP MVYDESRCVI ETTRGTFPVP DNYKKYKTLA
60 70 80 90 100
FAFVGHVLNT DDTPVIEKEL DWPDPALVYN TIVDRIINHP ELSQFISVAF
110 120 130 140 150
ISQLKATIGE GLDINVKGTL NRRGKGIRRP KGVFFRYMES PFVNTKVTAF
160 170 180 190 200
FSYLRDYNKI ASEYHNNTKF ILTFSCQAYW ASGPNFSALK NVIRCSIIHE
210 220 230 240 250
YISKFVEREQ DKGHIGDQEL PPEEDPSREL NNVQHEVNSL TEQDAEADEG
260 270 280 290 300
LWGEIDSLCE KWQSEAEDQT EAEIIADRII GNSQRMANLK IRRTKFKSVL
310 320 330 340 350
YHILKELIQS QGTVKVYRGS SFSHDSIKIS LHYEEQHITA VWVYLTVKFE
360 370
EHWKPVDVEV EFRCKFKERK VDG
Length:373
Mass (Da):43,231
Last modified:July 21, 1986 - v1
Checksum:iFD52FC966A3BA11E
GO

Sequence cautioni

The sequence AAA34966.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA39080.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti8 – 81A → V in strain: ATCC 44827 and ATCC 7754.
Natural varianti22 – 221V → I in strain: ATCC 44827 and ATCC 7754.
Natural varianti38 – 381P → S in strain: ATCC 44827 and ATCC 7754.
Natural varianti87 – 871I → R in strain: ATCC 44827 and ATCC 7754.
Natural varianti107 – 1071T → A in strain: ATCC 44827 and ATCC 7754.
Natural varianti111 – 12313GLDIN…TLNRR → VRYQCKRHVKPQ in strain: ATCC 44827.
Add
BLAST
Natural varianti117 – 1171K → R in strain: ATCC 7754.
Natural varianti131 – 1322KG → N in strain: ATCC 44827.
Natural varianti149 – 1491A → D in strain: ATCC 44827 and ATCC 7754.
Natural varianti177 – 1771Q → K in strain: ATCC 44827 and ATCC 7754.
Natural varianti184 – 1841P → Q in strain: ATCC 44827 and ATCC 7754.
Natural varianti198 – 1981I → V in strain: ATCC 44827 and ATCC 7754.
Natural varianti204 – 2041K → R in strain: ATCC 44827 and ATCC 7754.
Natural varianti211 – 2144DKGH → GL in strain: ATCC 44827 and ATCC 7754.
Natural varianti220 – 2223LPP → QPR in strain: ATCC 44827.
Natural varianti225 – 2284DPSR → NSSP in strain: ATCC 44827.
Natural varianti231 – 2311N → S in strain: ATCC 44827.
Natural varianti239 – 2413SLT → NLI in strain: ATCC 44827 and ATCC 7754.
Natural varianti246 – 2461E → A in strain: ATCC 44827 and ATCC 7754.
Natural varianti253 – 2531G → S in strain: ATCC 44827 and ATCC 7754.
Natural varianti261 – 2611K → R in strain: ATCC 7754.
Natural varianti270 – 2701T → A in strain: ATCC 44827 and ATCC 7754.
Natural varianti372 – 3732DG → NE in strain: ATCC 44827 and ATCC 7754.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01347 Genomic DNA. Translation: AAB59341.1.
M31942 Genomic DNA. Translation: AAA34966.1. Different initiation.
X55437 Genomic DNA. Translation: CAA39080.1. Different initiation.
PIRiA04503. PDBYB.
S11187.
S40417.
RefSeqiNP_040489.1. NC_001398.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01347 Genomic DNA. Translation: AAB59341.1 .
M31942 Genomic DNA. Translation: AAA34966.1 . Different initiation.
X55437 Genomic DNA. Translation: CAA39080.1 . Different initiation.
PIRi A04503. PDBYB.
S11187.
S40417.
RefSeqi NP_040489.1. NC_001398.1.

3D structure databases

ProteinModelPortali P03871.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-7698N.
IntActi P03871. 7 interactions.
MINTi MINT-687041.
STRINGi 4932.R0020C.

Proteomic databases

MaxQBi P03871.
PaxDbi P03871.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

CYGDi R0020c.
SGDi S000029675. REP1.

Phylogenomic databases

eggNOGi NOG326131.
InParanoidi P03871.
OrthoDBi EOG7B31XF.

Gene expression databases

Genevestigatori P03871.

Family and domain databases

InterProi IPR008897. Yeast_TAF.
[Graphical view ]
Pfami PF05797. Rep_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the yeast plasmid."
    Hartley J.L., Donelson J.E.
    Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A364A D5.
  2. "Heterogeneity among the 2 microns plasmids in Saccharomyces cerevisiae: a new sequence for the REP1 gene."
    Neuville P., Bonneu M., Aigle M.
    Gene 89:139-144(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 44827 / SKQ2N.
  3. "Evidence for cis- and trans-acting element coevolution of the 2-microns circle genome in Saccharomyces cerevisiae."
    Xiao W., Pelcher L.E., Rank G.H.
    J. Mol. Evol. 32:145-152(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 7754.
  4. "Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 micrometer plasmid."
    Hindley J., Phear G.A.
    Nucleic Acids Res. 7:361-375(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 330-373.
  5. "Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
    Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
    EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The 2 micrometer-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus."
    Ahn Y.-T., Wu X.-L., Biswal S., Velmurugan S., Volkert F.C., Jayaram M.
    J. Bacteriol. 179:7497-7506(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH REP2.
  7. "The 2 micrometer plasmid stability system: analyses of the interactions among plasmid- and host-encoded components."
    Velmurugan S., Ahn Y.-T., Yang X.-M., Wu X.-L., Jayaram M.
    Mol. Cell. Biol. 18:7466-7477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Partitioning of the 2-micrometer circle plasmid of Saccharomyces cerevisiae. Functional coordination with chromosome segregation and plasmid-encoded Rep protein distribution."
    Velmurugan S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M.
    J. Cell Biol. 149:553-566(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. Cited for: INTERACTION WITH REP2.
  10. "The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?"
    Mehta S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M., Velmurugan S.
    J. Cell Biol. 158:625-637(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Mutations in a partitioning protein and altered chromatin structure at the partitioning locus prevent cohesin recruitment by the Saccharomyces cerevisiae plasmid and cause plasmid missegregation."
    Yang X.-M., Mehta S., Uzri D., Jayaram M., Velmurugan S.
    Mol. Cell. Biol. 24:5290-5303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH REP2 AND STB, MUTAGENESIS OF THR-32; TYR-43; ALA-50; VAL-78; SER-93; LEU-154; GLU-200; ALA-276; LYS-297; TYR-301; ILE-308; TYR-317 AND SER-330.
  12. "The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-micrometer circle partitioning locus and promotes equal plasmid segregation."
    Hajra S., Ghosh S.K., Jayaram M.
    J. Cell Biol. 174:779-790(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiREP1_YEAST
AccessioniPrimary (citable) accession number: P03871
Secondary accession number(s): Q04137, Q07007
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

External Data

Dasty 3