Partitioning protein REP1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P03871 (REP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 83. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Partitioning protein REP1
Short name=R1

Alternative name(s):
Protein Baker
Trans-acting factor B

Gene names

Name:	REP1
Ordered Locus Names:	R0020C

Encoded on

Plasmid 2-micron

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1. Ref.5 Ref.8 Ref.10 Ref.11 Ref.12
Subunit structure	Interacts with REP2. Ref.6 Ref.9 Ref.11
Subcellular location	Nucleus. Note: Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases. Ref.6 Ref.7 Ref.8
Miscellaneous	The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.
Sequence caution	The sequence AAA34966.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA39080.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Plasmid partition
Cellular component	Nucleus
Technical term	Complete proteome Plasmid Reference proteome
Gene Ontology (GO)
Biological_process	2-micrometer plasmid partitioning Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	nucleus Inferred from direct assay PubMed 3542994 Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		5	EBI-14929,EBI-14929
REP2	P03872	7	EBI-14929,EBI-2125362

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 373

373

Partitioning protein REP1

PRO_0000150892

Regions

Region

1 – 129

129

Interaction with REP2 and self-association

Region

1 – 76

Interaction with REP2

Region

349 – 373

Nuclear localization

Natural variations

Natural variant

A → V in strain: ATCC 44827 and ATCC 7754.

Natural variant

V → I in strain: ATCC 44827 and ATCC 7754.

Natural variant

P → S in strain: ATCC 44827 and ATCC 7754.

Natural variant

I → R in strain: ATCC 44827 and ATCC 7754.

Natural variant

107

T → A in strain: ATCC 44827 and ATCC 7754.

Natural variant

111 – 123

GLDIN…TLNRR → VRYQCKRHVKPQ in strain: ATCC 44827.

Natural variant

117

K → R in strain: ATCC 7754.

Natural variant

131 – 132

KG → N in strain: ATCC 44827.

Natural variant

149

A → D in strain: ATCC 44827 and ATCC 7754.

Natural variant

177

Q → K in strain: ATCC 44827 and ATCC 7754.

Natural variant

184

P → Q in strain: ATCC 44827 and ATCC 7754.

Natural variant

198

I → V in strain: ATCC 44827 and ATCC 7754.

Natural variant

204

K → R in strain: ATCC 44827 and ATCC 7754.

Natural variant

211 – 214

DKGH → GL in strain: ATCC 44827 and ATCC 7754.

Natural variant

220 – 222

LPP → QPR in strain: ATCC 44827.

Natural variant

225 – 228

DPSR → NSSP in strain: ATCC 44827.

Natural variant

231

N → S in strain: ATCC 44827.

Natural variant

239 – 241

SLT → NLI in strain: ATCC 44827 and ATCC 7754.

Natural variant

246

E → A in strain: ATCC 44827 and ATCC 7754.

Natural variant

253

G → S in strain: ATCC 44827 and ATCC 7754.

Natural variant

261

K → R in strain: ATCC 7754.

Natural variant

270

T → A in strain: ATCC 44827 and ATCC 7754.

Natural variant

372 – 373

DG → NE in strain: ATCC 44827 and ATCC 7754.

Experimental info

Mutagenesis

T → K: Abolishes interaction with REP2 and STB. Ref.11

Mutagenesis

Y → A: Abolishes interaction with REP2, but not with STB. Ref.11

Mutagenesis

A → D: Abolishes interaction with REP2, but not with STB. Ref.11

Mutagenesis

V → K: Abolishes interaction with REP2, but not with STB. Ref.11

Mutagenesis

S → Y: Abolishes interaction with REP2, but not with STB. Ref.11

Mutagenesis

154

L → S: Abolishes interaction with STB, but not with REP2. Ref.11

Mutagenesis

200

E → A: Abolishes interaction with REP2, but not with STB. Ref.11

Mutagenesis

276

A → P: Abolishes interaction with REP2, but not with STB. Ref.11

Mutagenesis

297

K → Q: Abolishes interaction with STB, but not with REP2. Ref.11

Mutagenesis

301

Y → L: Abolishes interaction with REP2, but not with STB. Ref.11

Mutagenesis

308

I → Y: Abolishes interaction with REP2, but not with STB. Ref.11

Mutagenesis

317

Y → I: Abolishes interaction with STB, but not with REP2. Ref.11

Mutagenesis

330

S → Y: Abolishes interaction with STB, but not with REP2. Ref.11

Sequences

Sequence

Length

Mass (Da)

Tools

P03871 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: FD52FC966A3BA11E
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FASTA

373

43,231

        10         20         30         40         50         60 
MNGERLLACI KQCIMQHFQP MVYDESRCVI ETTRGTFPVP DNYKKYKTLA FAFVGHVLNT 

        70         80         90        100        110        120 
DDTPVIEKEL DWPDPALVYN TIVDRIINHP ELSQFISVAF ISQLKATIGE GLDINVKGTL 

       130        140        150        160        170        180 
NRRGKGIRRP KGVFFRYMES PFVNTKVTAF FSYLRDYNKI ASEYHNNTKF ILTFSCQAYW 

       190        200        210        220        230        240 
ASGPNFSALK NVIRCSIIHE YISKFVEREQ DKGHIGDQEL PPEEDPSREL NNVQHEVNSL 

       250        260        270        280        290        300 
TEQDAEADEG LWGEIDSLCE KWQSEAEDQT EAEIIADRII GNSQRMANLK IRRTKFKSVL 

       310        320        330        340        350        360 
YHILKELIQS QGTVKVYRGS SFSHDSIKIS LHYEEQHITA VWVYLTVKFE EHWKPVDVEV 

       370 
EFRCKFKERK VDG

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References

[1]	"Nucleotide sequence of the yeast plasmid." Hartley J.L., Donelson J.E. Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A364A D5.
[2]	"Heterogeneity among the 2 microns plasmids in Saccharomyces cerevisiae: a new sequence for the REP1 gene." Neuville P., Bonneu M., Aigle M. Gene 89:139-144(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 44827 / SKQ2N.
[3]	"Evidence for cis- and trans-acting element coevolution of the 2-microns circle genome in Saccharomyces cerevisiae." Xiao W., Pelcher L.E., Rank G.H. J. Mol. Evol. 32:145-152(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 7754.
[4]	"Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 micrometer plasmid." Hindley J., Phear G.A. Nucleic Acids Res. 7:361-375(1979) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 330-373.
[5]	"Antagonistic controls regulate copy number of the yeast 2 micron plasmid." Murray J.A.H., Scarpa M., Rossi N., Cesareni G. EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"The 2 micrometer-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus." Ahn Y.-T., Wu X.-L., Biswal S., Velmurugan S., Volkert F.C., Jayaram M. J. Bacteriol. 179:7497-7506(1997) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH REP2.
[7]	"The 2 micrometer plasmid stability system: analyses of the interactions among plasmid- and host-encoded components." Velmurugan S., Ahn Y.-T., Yang X.-M., Wu X.-L., Jayaram M. Mol. Cell. Biol. 18:7466-7477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Partitioning of the 2-micrometer circle plasmid of Saccharomyces cerevisiae. Functional coordination with chromosome segregation and plasmid-encoded Rep protein distribution." Velmurugan S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M. J. Cell Biol. 149:553-566(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Functional domains of yeast plasmid-encoded Rep proteins." Sengupta A., Blomqvist K., Pickett A.J., Zhang Y., Chew J.S.K., Dobson M.J. J. Bacteriol. 183:2306-2315(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH REP2.
[10]	"The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?" Mehta S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M., Velmurugan S. J. Cell Biol. 158:625-637(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Mutations in a partitioning protein and altered chromatin structure at the partitioning locus prevent cohesin recruitment by the Saccharomyces cerevisiae plasmid and cause plasmid missegregation." Yang X.-M., Mehta S., Uzri D., Jayaram M., Velmurugan S. Mol. Cell. Biol. 24:5290-5303(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH REP2 AND STB, MUTAGENESIS OF THR-32; TYR-43; ALA-50; VAL-78; SER-93; LEU-154; GLU-200; ALA-276; LYS-297; TYR-301; ILE-308; TYR-317 AND SER-330.
[12]	"The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-micrometer circle partitioning locus and promotes equal plasmid segregation." Hajra S., Ghosh S.K., Jayaram M. J. Cell Biol. 174:779-790(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01347 Genomic DNA. Translation: AAB59341.1. M31942 Genomic DNA. Translation: AAA34966.1. Different initiation. X55437 Genomic DNA. Translation: CAA39080.1. Different initiation.
PIR	PDBYB. A04503. S11187. S40417.
RefSeq	NP_040489.1. NC_001398.1.
3D structure databases
ProteinModelPortal	P03871.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7698N.
IntAct	P03871. 10 interactions.
MINT	MINT-687041.
STRING	4932.R0020C.
Proteomic databases
PaxDb	P03871.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
CYGD	R0020c.
SGD	S000029675. REP1.
Phylogenomic databases
eggNOG	NOG326131.
Gene expression databases
Genevestigator	P03871.
Family and domain databases
InterPro	IPR008897. Yeast_TAF. [Graphical view]
Pfam	PF05797. Rep_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

REP1_YEAST

Accession

Primary (citable) accession number: P03871
Secondary accession number(s): Q04137, Q07007

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents