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P03871 (REP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Partitioning protein REP1

Short name=R1
Alternative name(s):
Protein Baker
Trans-acting factor B
Gene names
Name:REP1
Ordered Locus Names:R0020C
Encoded onPlasmid 2-micron
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1. Ref.5 Ref.8 Ref.10 Ref.11 Ref.12

Subunit structure

Interacts with REP2. Ref.6 Ref.9 Ref.11

Subcellular location

Nucleus. Note: Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases. Ref.6 Ref.7 Ref.8

Miscellaneous

The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

Sequence caution

The sequence AAA34966.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA39080.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself5EBI-14929,EBI-14929
REP2P038727EBI-14929,EBI-2125362

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Partitioning protein REP1
PRO_0000150892

Regions

Region1 – 129129Interaction with REP2 and self-association
Region1 – 7676Interaction with REP2
Region349 – 37325Nuclear localization

Natural variations

Natural variant81A → V in strain: ATCC 44827 and ATCC 7754.
Natural variant221V → I in strain: ATCC 44827 and ATCC 7754.
Natural variant381P → S in strain: ATCC 44827 and ATCC 7754.
Natural variant871I → R in strain: ATCC 44827 and ATCC 7754.
Natural variant1071T → A in strain: ATCC 44827 and ATCC 7754.
Natural variant111 – 12313GLDIN…TLNRR → VRYQCKRHVKPQ in strain: ATCC 44827.
Natural variant1171K → R in strain: ATCC 7754.
Natural variant131 – 1322KG → N in strain: ATCC 44827.
Natural variant1491A → D in strain: ATCC 44827 and ATCC 7754.
Natural variant1771Q → K in strain: ATCC 44827 and ATCC 7754.
Natural variant1841P → Q in strain: ATCC 44827 and ATCC 7754.
Natural variant1981I → V in strain: ATCC 44827 and ATCC 7754.
Natural variant2041K → R in strain: ATCC 44827 and ATCC 7754.
Natural variant211 – 2144DKGH → GL in strain: ATCC 44827 and ATCC 7754.
Natural variant220 – 2223LPP → QPR in strain: ATCC 44827.
Natural variant225 – 2284DPSR → NSSP in strain: ATCC 44827.
Natural variant2311N → S in strain: ATCC 44827.
Natural variant239 – 2413SLT → NLI in strain: ATCC 44827 and ATCC 7754.
Natural variant2461E → A in strain: ATCC 44827 and ATCC 7754.
Natural variant2531G → S in strain: ATCC 44827 and ATCC 7754.
Natural variant2611K → R in strain: ATCC 7754.
Natural variant2701T → A in strain: ATCC 44827 and ATCC 7754.
Natural variant372 – 3732DG → NE in strain: ATCC 44827 and ATCC 7754.

Experimental info

Mutagenesis321T → K: Abolishes interaction with REP2 and STB. Ref.11
Mutagenesis431Y → A: Abolishes interaction with REP2, but not with STB. Ref.11
Mutagenesis501A → D: Abolishes interaction with REP2, but not with STB. Ref.11
Mutagenesis781V → K: Abolishes interaction with REP2, but not with STB. Ref.11
Mutagenesis931S → Y: Abolishes interaction with REP2, but not with STB. Ref.11
Mutagenesis1541L → S: Abolishes interaction with STB, but not with REP2. Ref.11
Mutagenesis2001E → A: Abolishes interaction with REP2, but not with STB. Ref.11
Mutagenesis2761A → P: Abolishes interaction with REP2, but not with STB. Ref.11
Mutagenesis2971K → Q: Abolishes interaction with STB, but not with REP2. Ref.11
Mutagenesis3011Y → L: Abolishes interaction with REP2, but not with STB. Ref.11
Mutagenesis3081I → Y: Abolishes interaction with REP2, but not with STB. Ref.11
Mutagenesis3171Y → I: Abolishes interaction with STB, but not with REP2. Ref.11
Mutagenesis3301S → Y: Abolishes interaction with STB, but not with REP2. Ref.11

Sequences

Sequence LengthMass (Da)Tools
P03871 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: FD52FC966A3BA11E

FASTA37343,231
        10         20         30         40         50         60 
MNGERLLACI KQCIMQHFQP MVYDESRCVI ETTRGTFPVP DNYKKYKTLA FAFVGHVLNT 

        70         80         90        100        110        120 
DDTPVIEKEL DWPDPALVYN TIVDRIINHP ELSQFISVAF ISQLKATIGE GLDINVKGTL 

       130        140        150        160        170        180 
NRRGKGIRRP KGVFFRYMES PFVNTKVTAF FSYLRDYNKI ASEYHNNTKF ILTFSCQAYW 

       190        200        210        220        230        240 
ASGPNFSALK NVIRCSIIHE YISKFVEREQ DKGHIGDQEL PPEEDPSREL NNVQHEVNSL 

       250        260        270        280        290        300 
TEQDAEADEG LWGEIDSLCE KWQSEAEDQT EAEIIADRII GNSQRMANLK IRRTKFKSVL 

       310        320        330        340        350        360 
YHILKELIQS QGTVKVYRGS SFSHDSIKIS LHYEEQHITA VWVYLTVKFE EHWKPVDVEV 

       370 
EFRCKFKERK VDG 

« Hide

References

[1]"Nucleotide sequence of the yeast plasmid."
Hartley J.L., Donelson J.E.
Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A364A D5.
[2]"Heterogeneity among the 2 microns plasmids in Saccharomyces cerevisiae: a new sequence for the REP1 gene."
Neuville P., Bonneu M., Aigle M.
Gene 89:139-144(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 44827 / SKQ2N.
[3]"Evidence for cis- and trans-acting element coevolution of the 2-microns circle genome in Saccharomyces cerevisiae."
Xiao W., Pelcher L.E., Rank G.H.
J. Mol. Evol. 32:145-152(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 7754.
[4]"Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 micrometer plasmid."
Hindley J., Phear G.A.
Nucleic Acids Res. 7:361-375(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 330-373.
[5]"Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The 2 micrometer-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus."
Ahn Y.-T., Wu X.-L., Biswal S., Velmurugan S., Volkert F.C., Jayaram M.
J. Bacteriol. 179:7497-7506(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH REP2.
[7]"The 2 micrometer plasmid stability system: analyses of the interactions among plasmid- and host-encoded components."
Velmurugan S., Ahn Y.-T., Yang X.-M., Wu X.-L., Jayaram M.
Mol. Cell. Biol. 18:7466-7477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"Partitioning of the 2-micrometer circle plasmid of Saccharomyces cerevisiae. Functional coordination with chromosome segregation and plasmid-encoded Rep protein distribution."
Velmurugan S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M.
J. Cell Biol. 149:553-566(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]"Functional domains of yeast plasmid-encoded Rep proteins."
Sengupta A., Blomqvist K., Pickett A.J., Zhang Y., Chew J.S.K., Dobson M.J.
J. Bacteriol. 183:2306-2315(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH REP2.
[10]"The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?"
Mehta S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M., Velmurugan S.
J. Cell Biol. 158:625-637(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Mutations in a partitioning protein and altered chromatin structure at the partitioning locus prevent cohesin recruitment by the Saccharomyces cerevisiae plasmid and cause plasmid missegregation."
Yang X.-M., Mehta S., Uzri D., Jayaram M., Velmurugan S.
Mol. Cell. Biol. 24:5290-5303(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH REP2 AND STB, MUTAGENESIS OF THR-32; TYR-43; ALA-50; VAL-78; SER-93; LEU-154; GLU-200; ALA-276; LYS-297; TYR-301; ILE-308; TYR-317 AND SER-330.
[12]"The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-micrometer circle partitioning locus and promotes equal plasmid segregation."
Hajra S., Ghosh S.K., Jayaram M.
J. Cell Biol. 174:779-790(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J01347 Genomic DNA. Translation: AAB59341.1.
M31942 Genomic DNA. Translation: AAA34966.1. Different initiation.
X55437 Genomic DNA. Translation: CAA39080.1. Different initiation.
PIRPDBYB. A04503.
S11187.
S40417.
RefSeqNP_040489.1. NC_001398.1.

3D structure databases

ProteinModelPortalP03871.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-7698N.
IntActP03871. 7 interactions.
MINTMINT-687041.
STRING4932.R0020C.

Proteomic databases

PaxDbP03871.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

CYGDR0020c.
SGDS000029675. REP1.

Phylogenomic databases

eggNOGNOG326131.
OrthoDBEOG7B31XF.

Gene expression databases

GenevestigatorP03871.

Family and domain databases

InterProIPR008897. Yeast_TAF.
[Graphical view]
PfamPF05797. Rep_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameREP1_YEAST
AccessionPrimary (citable) accession number: P03871
Secondary accession number(s): Q04137, Q07007
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD