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P03871

- REP1_YEAST

UniProt

P03871 - REP1_YEAST

Protein

Partitioning protein REP1

Gene

REP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1.5 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct

    GO - Biological processi

    1. 2-micrometer plasmid partitioning Source: SGD
    2. cellular protein localization Source: SGD

    Keywords - Biological processi

    Plasmid partition

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Partitioning protein REP1
    Short name:
    R1
    Alternative name(s):
    Protein Baker
    Trans-acting factor B
    Gene namesi
    Name:REP1
    Ordered Locus Names:R0020C
    Encoded oniPlasmid 2-micron0 Publication
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Plasmid 2-micron

    Organism-specific databases

    CYGDiR0020c.
    SGDiS000029675. REP1.

    Subcellular locationi

    Nucleus 3 Publications
    Note: Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases.

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi32 – 321T → K: Abolishes interaction with REP2 and STB. 1 Publication
    Mutagenesisi43 – 431Y → A: Abolishes interaction with REP2, but not with STB. 1 Publication
    Mutagenesisi50 – 501A → D: Abolishes interaction with REP2, but not with STB. 1 Publication
    Mutagenesisi78 – 781V → K: Abolishes interaction with REP2, but not with STB. 1 Publication
    Mutagenesisi93 – 931S → Y: Abolishes interaction with REP2, but not with STB. 1 Publication
    Mutagenesisi154 – 1541L → S: Abolishes interaction with STB, but not with REP2. 1 Publication
    Mutagenesisi200 – 2001E → A: Abolishes interaction with REP2, but not with STB. 1 Publication
    Mutagenesisi276 – 2761A → P: Abolishes interaction with REP2, but not with STB. 1 Publication
    Mutagenesisi297 – 2971K → Q: Abolishes interaction with STB, but not with REP2. 1 Publication
    Mutagenesisi301 – 3011Y → L: Abolishes interaction with REP2, but not with STB. 1 Publication
    Mutagenesisi308 – 3081I → Y: Abolishes interaction with REP2, but not with STB. 1 Publication
    Mutagenesisi317 – 3171Y → I: Abolishes interaction with STB, but not with REP2. 1 Publication
    Mutagenesisi330 – 3301S → Y: Abolishes interaction with STB, but not with REP2. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 373373Partitioning protein REP1PRO_0000150892Add
    BLAST

    Proteomic databases

    MaxQBiP03871.
    PaxDbiP03871.

    Expressioni

    Gene expression databases

    GenevestigatoriP03871.

    Interactioni

    Subunit structurei

    Interacts with REP2.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself5EBI-14929,EBI-14929
    REP2P038727EBI-14929,EBI-2125362

    Protein-protein interaction databases

    DIPiDIP-7698N.
    IntActiP03871. 7 interactions.
    MINTiMINT-687041.
    STRINGi4932.R0020C.

    Structurei

    3D structure databases

    ProteinModelPortaliP03871.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 129129Interaction with REP2 and self-associationAdd
    BLAST
    Regioni1 – 7676Interaction with REP2Add
    BLAST
    Regioni349 – 37325Nuclear localizationAdd
    BLAST

    Phylogenomic databases

    eggNOGiNOG326131.
    OrthoDBiEOG7B31XF.

    Family and domain databases

    InterProiIPR008897. Yeast_TAF.
    [Graphical view]
    PfamiPF05797. Rep_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03871-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGERLLACI KQCIMQHFQP MVYDESRCVI ETTRGTFPVP DNYKKYKTLA    50
    FAFVGHVLNT DDTPVIEKEL DWPDPALVYN TIVDRIINHP ELSQFISVAF 100
    ISQLKATIGE GLDINVKGTL NRRGKGIRRP KGVFFRYMES PFVNTKVTAF 150
    FSYLRDYNKI ASEYHNNTKF ILTFSCQAYW ASGPNFSALK NVIRCSIIHE 200
    YISKFVEREQ DKGHIGDQEL PPEEDPSREL NNVQHEVNSL TEQDAEADEG 250
    LWGEIDSLCE KWQSEAEDQT EAEIIADRII GNSQRMANLK IRRTKFKSVL 300
    YHILKELIQS QGTVKVYRGS SFSHDSIKIS LHYEEQHITA VWVYLTVKFE 350
    EHWKPVDVEV EFRCKFKERK VDG 373
    Length:373
    Mass (Da):43,231
    Last modified:July 21, 1986 - v1
    Checksum:iFD52FC966A3BA11E
    GO

    Sequence cautioni

    The sequence AAA34966.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA39080.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti8 – 81A → V in strain: ATCC 44827 and ATCC 7754.
    Natural varianti22 – 221V → I in strain: ATCC 44827 and ATCC 7754.
    Natural varianti38 – 381P → S in strain: ATCC 44827 and ATCC 7754.
    Natural varianti87 – 871I → R in strain: ATCC 44827 and ATCC 7754.
    Natural varianti107 – 1071T → A in strain: ATCC 44827 and ATCC 7754.
    Natural varianti111 – 12313GLDIN…TLNRR → VRYQCKRHVKPQ in strain: ATCC 44827.
    Add
    BLAST
    Natural varianti117 – 1171K → R in strain: ATCC 7754.
    Natural varianti131 – 1322KG → N in strain: ATCC 44827.
    Natural varianti149 – 1491A → D in strain: ATCC 44827 and ATCC 7754.
    Natural varianti177 – 1771Q → K in strain: ATCC 44827 and ATCC 7754.
    Natural varianti184 – 1841P → Q in strain: ATCC 44827 and ATCC 7754.
    Natural varianti198 – 1981I → V in strain: ATCC 44827 and ATCC 7754.
    Natural varianti204 – 2041K → R in strain: ATCC 44827 and ATCC 7754.
    Natural varianti211 – 2144DKGH → GL in strain: ATCC 44827 and ATCC 7754.
    Natural varianti220 – 2223LPP → QPR in strain: ATCC 44827.
    Natural varianti225 – 2284DPSR → NSSP in strain: ATCC 44827.
    Natural varianti231 – 2311N → S in strain: ATCC 44827.
    Natural varianti239 – 2413SLT → NLI in strain: ATCC 44827 and ATCC 7754.
    Natural varianti246 – 2461E → A in strain: ATCC 44827 and ATCC 7754.
    Natural varianti253 – 2531G → S in strain: ATCC 44827 and ATCC 7754.
    Natural varianti261 – 2611K → R in strain: ATCC 7754.
    Natural varianti270 – 2701T → A in strain: ATCC 44827 and ATCC 7754.
    Natural varianti372 – 3732DG → NE in strain: ATCC 44827 and ATCC 7754.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01347 Genomic DNA. Translation: AAB59341.1.
    M31942 Genomic DNA. Translation: AAA34966.1. Different initiation.
    X55437 Genomic DNA. Translation: CAA39080.1. Different initiation.
    PIRiA04503. PDBYB.
    S11187.
    S40417.
    RefSeqiNP_040489.1. NC_001398.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01347 Genomic DNA. Translation: AAB59341.1 .
    M31942 Genomic DNA. Translation: AAA34966.1 . Different initiation.
    X55437 Genomic DNA. Translation: CAA39080.1 . Different initiation.
    PIRi A04503. PDBYB.
    S11187.
    S40417.
    RefSeqi NP_040489.1. NC_001398.1.

    3D structure databases

    ProteinModelPortali P03871.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-7698N.
    IntActi P03871. 7 interactions.
    MINTi MINT-687041.
    STRINGi 4932.R0020C.

    Proteomic databases

    MaxQBi P03871.
    PaxDbi P03871.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    CYGDi R0020c.
    SGDi S000029675. REP1.

    Phylogenomic databases

    eggNOGi NOG326131.
    OrthoDBi EOG7B31XF.

    Gene expression databases

    Genevestigatori P03871.

    Family and domain databases

    InterProi IPR008897. Yeast_TAF.
    [Graphical view ]
    Pfami PF05797. Rep_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the yeast plasmid."
      Hartley J.L., Donelson J.E.
      Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A364A D5.
    2. "Heterogeneity among the 2 microns plasmids in Saccharomyces cerevisiae: a new sequence for the REP1 gene."
      Neuville P., Bonneu M., Aigle M.
      Gene 89:139-144(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 44827 / SKQ2N.
    3. "Evidence for cis- and trans-acting element coevolution of the 2-microns circle genome in Saccharomyces cerevisiae."
      Xiao W., Pelcher L.E., Rank G.H.
      J. Mol. Evol. 32:145-152(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 7754.
    4. "Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 micrometer plasmid."
      Hindley J., Phear G.A.
      Nucleic Acids Res. 7:361-375(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 330-373.
    5. "Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
      Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
      EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The 2 micrometer-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus."
      Ahn Y.-T., Wu X.-L., Biswal S., Velmurugan S., Volkert F.C., Jayaram M.
      J. Bacteriol. 179:7497-7506(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH REP2.
    7. "The 2 micrometer plasmid stability system: analyses of the interactions among plasmid- and host-encoded components."
      Velmurugan S., Ahn Y.-T., Yang X.-M., Wu X.-L., Jayaram M.
      Mol. Cell. Biol. 18:7466-7477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Partitioning of the 2-micrometer circle plasmid of Saccharomyces cerevisiae. Functional coordination with chromosome segregation and plasmid-encoded Rep protein distribution."
      Velmurugan S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M.
      J. Cell Biol. 149:553-566(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    9. Cited for: INTERACTION WITH REP2.
    10. "The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?"
      Mehta S., Yang X.-M., Chan C.S.-M., Dobson M.J., Jayaram M., Velmurugan S.
      J. Cell Biol. 158:625-637(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Mutations in a partitioning protein and altered chromatin structure at the partitioning locus prevent cohesin recruitment by the Saccharomyces cerevisiae plasmid and cause plasmid missegregation."
      Yang X.-M., Mehta S., Uzri D., Jayaram M., Velmurugan S.
      Mol. Cell. Biol. 24:5290-5303(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH REP2 AND STB, MUTAGENESIS OF THR-32; TYR-43; ALA-50; VAL-78; SER-93; LEU-154; GLU-200; ALA-276; LYS-297; TYR-301; ILE-308; TYR-317 AND SER-330.
    12. "The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-micrometer circle partitioning locus and promotes equal plasmid segregation."
      Hajra S., Ghosh S.K., Jayaram M.
      J. Cell Biol. 174:779-790(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiREP1_YEAST
    AccessioniPrimary (citable) accession number: P03871
    Secondary accession number(s): Q04137, Q07007
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

    External Data

    Dasty 3