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Protein

Partitioning protein REP1

Gene

REP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1.5 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • 2-micrometer plasmid partitioning Source: SGD
  • cellular protein localization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Plasmid partition

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning protein REP1
Short name:
R1
Alternative name(s):
Protein Baker
Trans-acting factor B
Gene namesi
Name:REP1
Ordered Locus Names:R0020C
Encoded oniPlasmid 2-micron0 Publication
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Plasmid 2-micron

Organism-specific databases

SGDiS000029675. REP1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi32T → K: Abolishes interaction with REP2 and STB. 1 Publication1
Mutagenesisi43Y → A: Abolishes interaction with REP2, but not with STB. 1 Publication1
Mutagenesisi50A → D: Abolishes interaction with REP2, but not with STB. 1 Publication1
Mutagenesisi78V → K: Abolishes interaction with REP2, but not with STB. 1 Publication1
Mutagenesisi93S → Y: Abolishes interaction with REP2, but not with STB. 1 Publication1
Mutagenesisi154L → S: Abolishes interaction with STB, but not with REP2. 1 Publication1
Mutagenesisi200E → A: Abolishes interaction with REP2, but not with STB. 1 Publication1
Mutagenesisi276A → P: Abolishes interaction with REP2, but not with STB. 1 Publication1
Mutagenesisi297K → Q: Abolishes interaction with STB, but not with REP2. 1 Publication1
Mutagenesisi301Y → L: Abolishes interaction with REP2, but not with STB. 1 Publication1
Mutagenesisi308I → Y: Abolishes interaction with REP2, but not with STB. 1 Publication1
Mutagenesisi317Y → I: Abolishes interaction with STB, but not with REP2. 1 Publication1
Mutagenesisi330S → Y: Abolishes interaction with STB, but not with REP2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001508921 – 373Partitioning protein REP1Add BLAST373

Proteomic databases

MaxQBiP03871.
PRIDEiP03871.

Interactioni

Subunit structurei

Interacts with REP2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-14929,EBI-14929
REP2P038727EBI-14929,EBI-2125362

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-7698N.
IntActiP03871. 8 interactors.
MINTiMINT-687041.

Structurei

3D structure databases

ProteinModelPortaliP03871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 129Interaction with REP2 and self-associationAdd BLAST129
Regioni1 – 76Interaction with REP2Add BLAST76
Regioni349 – 373Nuclear localizationAdd BLAST25

Phylogenomic databases

InParanoidiP03871.
OrthoDBiEOG092C2LVW.

Family and domain databases

InterProiIPR008897. Rep_fungi.
[Graphical view]
PfamiPF05797. Rep_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGERLLACI KQCIMQHFQP MVYDESRCVI ETTRGTFPVP DNYKKYKTLA
60 70 80 90 100
FAFVGHVLNT DDTPVIEKEL DWPDPALVYN TIVDRIINHP ELSQFISVAF
110 120 130 140 150
ISQLKATIGE GLDINVKGTL NRRGKGIRRP KGVFFRYMES PFVNTKVTAF
160 170 180 190 200
FSYLRDYNKI ASEYHNNTKF ILTFSCQAYW ASGPNFSALK NVIRCSIIHE
210 220 230 240 250
YISKFVEREQ DKGHIGDQEL PPEEDPSREL NNVQHEVNSL TEQDAEADEG
260 270 280 290 300
LWGEIDSLCE KWQSEAEDQT EAEIIADRII GNSQRMANLK IRRTKFKSVL
310 320 330 340 350
YHILKELIQS QGTVKVYRGS SFSHDSIKIS LHYEEQHITA VWVYLTVKFE
360 370
EHWKPVDVEV EFRCKFKERK VDG
Length:373
Mass (Da):43,231
Last modified:July 21, 1986 - v1
Checksum:iFD52FC966A3BA11E
GO

Sequence cautioni

The sequence AAA34966 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA39080 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti8A → V in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti22V → I in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti38P → S in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti87I → R in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti107T → A in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti111 – 123GLDIN…TLNRR → VRYQCKRHVKPQ in strain: ATCC 44827. Add BLAST13
Natural varianti117K → R in strain: ATCC 7754. 1
Natural varianti131 – 132KG → N in strain: ATCC 44827. 2
Natural varianti149A → D in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti177Q → K in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti184P → Q in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti198I → V in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti204K → R in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti211 – 214DKGH → GL in strain: ATCC 44827 and ATCC 7754. 4
Natural varianti220 – 222LPP → QPR in strain: ATCC 44827. 3
Natural varianti225 – 228DPSR → NSSP in strain: ATCC 44827. 4
Natural varianti231N → S in strain: ATCC 44827. 1
Natural varianti239 – 241SLT → NLI in strain: ATCC 44827 and ATCC 7754. 3
Natural varianti246E → A in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti253G → S in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti261K → R in strain: ATCC 7754. 1
Natural varianti270T → A in strain: ATCC 44827 and ATCC 7754. 1
Natural varianti372 – 373DG → NE in strain: ATCC 44827 and ATCC 7754. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01347 Genomic DNA. Translation: AAB59341.1.
M31942 Genomic DNA. Translation: AAA34966.1. Different initiation.
X55437 Genomic DNA. Translation: CAA39080.1. Different initiation.
PIRiA04503. PDBYB.
S11187.
S40417.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01347 Genomic DNA. Translation: AAB59341.1.
M31942 Genomic DNA. Translation: AAA34966.1. Different initiation.
X55437 Genomic DNA. Translation: CAA39080.1. Different initiation.
PIRiA04503. PDBYB.
S11187.
S40417.

3D structure databases

ProteinModelPortaliP03871.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-7698N.
IntActiP03871. 8 interactors.
MINTiMINT-687041.

Proteomic databases

MaxQBiP03871.
PRIDEiP03871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

SGDiS000029675. REP1.

Phylogenomic databases

InParanoidiP03871.
OrthoDBiEOG092C2LVW.

Miscellaneous databases

PROiP03871.

Family and domain databases

InterProiIPR008897. Rep_fungi.
[Graphical view]
PfamiPF05797. Rep_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiREP1_YEAST
AccessioniPrimary (citable) accession number: P03871
Secondary accession number(s): Q04137, Q07007
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.