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P03870

- FLP_YEAST

UniProt

P03870 - FLP_YEAST

Protein

Site-specific recombinase Flp

Gene

FLP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c.3 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei343 – 3431O-(3'-phospho-DNA)-tyrosine intermediate1 Publication

    GO - Molecular functioni

    1. DNA binding, bending Source: SGD
    2. double-stranded DNA binding Source: SGD
    3. single-stranded DNA binding Source: SGD
    4. site-specific recombinase activity Source: SGD

    GO - Biological processi

    1. DNA integration Source: UniProtKB-KW
    2. plasmid recombination Source: SGD

    Keywords - Biological processi

    DNA integration, DNA recombination

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Site-specific recombinase Flp
    Short name:
    FLP
    Alternative name(s):
    Protein Able
    Gene namesi
    Name:FLP1
    Ordered Locus Names:R0010W
    Encoded oniPlasmid 2-micron0 Publication
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Plasmid 2-micron

    Organism-specific databases

    CYGDiR0010w.
    SGDiS000029654. FLP1.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi305 – 3051H → L or P: Inactive and weakened DNA binding. 1 Publication
    Mutagenesisi305 – 3051H → Q: Reduced activity. 1 Publication
    Mutagenesisi308 – 3081R → G: Inactive and weakened DNA binding. 1 Publication
    Mutagenesisi343 – 3431Y → F or S: No strand cleavage or recombination. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 423423Site-specific recombinase FlpPRO_0000197567Add
    BLAST

    Expressioni

    Inductioni

    Repressed by the negative regulatory complex REP1-REP2.1 Publication

    Gene expression databases

    GenevestigatoriP03870.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Structurei

    Secondary structure

    1
    423
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 97
    Helixi12 – 2413
    Helixi28 – 314
    Helixi32 – 343
    Helixi35 – 4612
    Turni47 – 493
    Helixi54 – 6512
    Beta strandi69 – 713
    Turni72 – 754
    Beta strandi76 – 805
    Helixi86 – 9611
    Beta strandi101 – 1055
    Helixi116 – 12813
    Helixi138 – 14912
    Beta strandi150 – 1523
    Helixi154 – 16310
    Turni164 – 1685
    Helixi172 – 18817
    Helixi192 – 1965
    Beta strandi199 – 2068
    Turni209 – 2113
    Beta strandi214 – 2196
    Beta strandi228 – 2325
    Beta strandi236 – 2383
    Helixi241 – 25111
    Beta strandi267 – 2693
    Beta strandi272 – 2754
    Helixi279 – 29012
    Helixi294 – 2974
    Helixi306 – 31712
    Helixi324 – 3285
    Helixi337 – 3404
    Turni341 – 3433
    Helixi352 – 3587
    Beta strandi361 – 3655
    Turni366 – 3694
    Beta strandi370 – 3734
    Helixi380 – 38910
    Helixi395 – 3995
    Helixi401 – 4033
    Turni404 – 4063
    Helixi409 – 42012

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FLOX-ray2.65A/B/C/D2-423[»]
    1M6XX-ray2.80A/B/C/D1-423[»]
    1P4EX-ray2.70A/B2-423[»]
    C/D2-422[»]
    ProteinModelPortaliP03870.
    SMRiP03870. Positions 2-422.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03870.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 'phage' integrase family.Curated

    Phylogenomic databases

    eggNOGiNOG255844.
    OrthoDBiEOG7TXKS7.

    Family and domain databases

    Gene3Di1.10.443.10. 1 hit.
    InterProiIPR011010. DNA_brk_join_enz.
    IPR013762. Integrase-like_cat-core.
    IPR010998. Integrase_Lambda-type_N.
    IPR005626. Recombinase_Flp.
    IPR022647. Recombinase_Flp_N.
    [Graphical view]
    PfamiPF05202. Flp_C. 1 hit.
    PF03930. Flp_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47823. SSF47823. 1 hit.
    SSF56349. SSF56349. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P03870-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPQFGILCKT PPKVLVRQFV ERFERPSGEK IALCAAELTY LCWMITHNGT    50
    AIKRATFMSY NTIISNSLSF DIVNKSLQFK YKTQKATILE ASLKKLIPAW 100
    EFTIIPYYGQ KHQSDITDIV SSLQLQFESS EEADKGNSHS KKMLKALLSE 150
    GESIWEITEK ILNSFEYTSR FTKTKTLYQF LFLATFINCG RFSDIKNVDP 200
    KSFKLVQNKY LGVIIQCLVT ETKTSVSRHI YFFSARGRID PLVYLDEFLR 250
    NSEPVLKRVN RTGNSSSNKQ EYQLLKDNLV RSYNKALKKN APYSIFAIKN 300
    GPKSHIGRHL MTSFLSMKGL TELTNVVGNW SDKRASAVAR TTYTHQITAI 350
    PDHYFALVSR YYAYDPISKE MIALKDETNP IEEWQHIEQL KGSAEGSIRY 400
    PAWNGIISQE VLDYLSSYIN RRI 423
    Length:423
    Mass (Da):48,620
    Last modified:July 21, 1986 - v1
    Checksum:iB9C3DE95AB253BC3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51G → D AA sequence (PubMed:2995370)Curated
    Sequence conflicti5 – 51G → D AA sequence (PubMed:2040639)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01347 Genomic DNA. Translation: AAB59340.1.
    PIRiA04502. PDBYA.
    RefSeqiNP_040488.1. NC_001398.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01347 Genomic DNA. Translation: AAB59340.1 .
    PIRi A04502. PDBYA.
    RefSeqi NP_040488.1. NC_001398.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FLO X-ray 2.65 A/B/C/D 2-423 [» ]
    1M6X X-ray 2.80 A/B/C/D 1-423 [» ]
    1P4E X-ray 2.70 A/B 2-423 [» ]
    C/D 2-422 [» ]
    ProteinModelPortali P03870.
    SMRi P03870. Positions 2-422.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    CYGDi R0010w.
    SGDi S000029654. FLP1.

    Phylogenomic databases

    eggNOGi NOG255844.
    OrthoDBi EOG7TXKS7.

    Miscellaneous databases

    EvolutionaryTracei P03870.

    Gene expression databases

    Genevestigatori P03870.

    Family and domain databases

    Gene3Di 1.10.443.10. 1 hit.
    InterProi IPR011010. DNA_brk_join_enz.
    IPR013762. Integrase-like_cat-core.
    IPR010998. Integrase_Lambda-type_N.
    IPR005626. Recombinase_Flp.
    IPR022647. Recombinase_Flp_N.
    [Graphical view ]
    Pfami PF05202. Flp_C. 1 hit.
    PF03930. Flp_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47823. SSF47823. 1 hit.
    SSF56349. SSF56349. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the yeast plasmid."
      Hartley J.L., Donelson J.E.
      Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A364A D5.
    2. "The FLP protein of the 2-micron plasmid of yeast. Purification of the protein from Escherichia coli cells expressing the cloned FLP gene."
      Babineau D., Vetter D., Andrews B.J., Gronostajski R.M., Proteau G.A., Beatty L.G., Sadowski P.D.
      J. Biol. Chem. 260:12313-12319(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-12.
    3. "Identification of the DNA-binding domain of the FLP recombinase."
      Pan H., Clary D., Sadowski P.D.
      J. Biol. Chem. 266:11347-11354(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-7; 124-129 AND 148-153, FUNCTION.
    4. "Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 micrometer plasmid."
      Hindley J., Phear G.A.
      Nucleic Acids Res. 7:361-375(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 250-423.
    5. "Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
      Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
      EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae."
      Reynolds A.E., Murray A.W.H., Szostak J.W.
      Mol. Cell. Biol. 7:3566-3573(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Mutations in the 2-microns circle site-specific recombinase that abolish recombination without affecting substrate recognition."
      Prasad P.V., Young L.J., Jayaram M.
      Proc. Natl. Acad. Sci. U.S.A. 84:2189-2193(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF TYR-343.
    8. "Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination."
      Parsons R.L., Prasad P.V., Harshey R.M., Jayaram M.
      Mol. Cell. Biol. 8:3303-3310(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF HIS-305 AND ARG-308.
    9. "FLP protein of 2 mu circle plasmid of yeast induces multiple bends in the FLP recognition target site."
      Schwartz C.J., Sadowski P.D.
      J. Mol. Biol. 216:289-298(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Identification of the active site tyrosine of Flp recombinase. Possible relevance of its location to the mechanism of recombination."
      Evans B.R., Chen J.W., Parsons R.L., Bauer T.K., Teplow D.B., Jayaram M.
      J. Biol. Chem. 265:18504-18510(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVE SITE.
    11. "Crystal structure of a Flp recombinase-Holliday junction complex: assembly of an active oligomer by helix swapping."
      Chen Y., Narendra U., Iype L.E., Cox M.M., Rice P.A.
      Mol. Cell 6:885-897(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) IN COMPLEX WITH DNA, SUBUNIT.

    Entry informationi

    Entry nameiFLP_YEAST
    AccessioniPrimary (citable) accession number: P03870
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

    External Data

    Dasty 3