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P03870 (FLP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Site-specific recombinase Flp

Short name=FLP
Alternative name(s):
Protein Able
Gene names
Name:FLP1
Ordered Locus Names:R0010W
Encoded onPlasmid 2-micron
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c. Ref.3 Ref.6 Ref.9

Subunit structure

Homotetramer. Ref.11

Induction

Repressed by the negative regulatory complex REP1-REP2. Ref.5

Miscellaneous

The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

Sequence similarities

Belongs to the 'phage' integrase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Site-specific recombinase Flp
PRO_0000197567

Sites

Active site3431O-(3'-phospho-DNA)-tyrosine intermediate Ref.10

Experimental info

Mutagenesis3051H → L or P: Inactive and weakened DNA binding. Ref.8
Mutagenesis3051H → Q: Reduced activity. Ref.8
Mutagenesis3081R → G: Inactive and weakened DNA binding. Ref.8
Mutagenesis3431Y → F or S: No strand cleavage or recombination. Ref.7
Sequence conflict51G → D AA sequence Ref.2
Sequence conflict51G → D AA sequence Ref.3

Secondary structure

.......................................................................... 423
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P03870 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: B9C3DE95AB253BC3

FASTA42348,620
        10         20         30         40         50         60 
MPQFGILCKT PPKVLVRQFV ERFERPSGEK IALCAAELTY LCWMITHNGT AIKRATFMSY 

        70         80         90        100        110        120 
NTIISNSLSF DIVNKSLQFK YKTQKATILE ASLKKLIPAW EFTIIPYYGQ KHQSDITDIV 

       130        140        150        160        170        180 
SSLQLQFESS EEADKGNSHS KKMLKALLSE GESIWEITEK ILNSFEYTSR FTKTKTLYQF 

       190        200        210        220        230        240 
LFLATFINCG RFSDIKNVDP KSFKLVQNKY LGVIIQCLVT ETKTSVSRHI YFFSARGRID 

       250        260        270        280        290        300 
PLVYLDEFLR NSEPVLKRVN RTGNSSSNKQ EYQLLKDNLV RSYNKALKKN APYSIFAIKN 

       310        320        330        340        350        360 
GPKSHIGRHL MTSFLSMKGL TELTNVVGNW SDKRASAVAR TTYTHQITAI PDHYFALVSR 

       370        380        390        400        410        420 
YYAYDPISKE MIALKDETNP IEEWQHIEQL KGSAEGSIRY PAWNGIISQE VLDYLSSYIN 


RRI 

« Hide

References

[1]"Nucleotide sequence of the yeast plasmid."
Hartley J.L., Donelson J.E.
Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A364A D5.
[2]"The FLP protein of the 2-micron plasmid of yeast. Purification of the protein from Escherichia coli cells expressing the cloned FLP gene."
Babineau D., Vetter D., Andrews B.J., Gronostajski R.M., Proteau G.A., Beatty L.G., Sadowski P.D.
J. Biol. Chem. 260:12313-12319(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-12.
[3]"Identification of the DNA-binding domain of the FLP recombinase."
Pan H., Clary D., Sadowski P.D.
J. Biol. Chem. 266:11347-11354(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-7; 124-129 AND 148-153, FUNCTION.
[4]"Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 micrometer plasmid."
Hindley J., Phear G.A.
Nucleic Acids Res. 7:361-375(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 250-423.
[5]"Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae."
Reynolds A.E., Murray A.W.H., Szostak J.W.
Mol. Cell. Biol. 7:3566-3573(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Mutations in the 2-microns circle site-specific recombinase that abolish recombination without affecting substrate recognition."
Prasad P.V., Young L.J., Jayaram M.
Proc. Natl. Acad. Sci. U.S.A. 84:2189-2193(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF TYR-343.
[8]"Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination."
Parsons R.L., Prasad P.V., Harshey R.M., Jayaram M.
Mol. Cell. Biol. 8:3303-3310(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF HIS-305 AND ARG-308.
[9]"FLP protein of 2 mu circle plasmid of yeast induces multiple bends in the FLP recognition target site."
Schwartz C.J., Sadowski P.D.
J. Mol. Biol. 216:289-298(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Identification of the active site tyrosine of Flp recombinase. Possible relevance of its location to the mechanism of recombination."
Evans B.R., Chen J.W., Parsons R.L., Bauer T.K., Teplow D.B., Jayaram M.
J. Biol. Chem. 265:18504-18510(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: ACTIVE SITE.
[11]"Crystal structure of a Flp recombinase-Holliday junction complex: assembly of an active oligomer by helix swapping."
Chen Y., Narendra U., Iype L.E., Cox M.M., Rice P.A.
Mol. Cell 6:885-897(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) IN COMPLEX WITH DNA, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J01347 Genomic DNA. Translation: AAB59340.1.
PIRPDBYA. A04502.
RefSeqNP_040488.1. NC_001398.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FLOX-ray2.65A/B/C/D2-423[»]
1M6XX-ray2.80A/B/C/D3-423[»]
1P4EX-ray2.70A/B3-423[»]
C/D3-422[»]
ProteinModelPortalP03870.
SMRP03870. Positions 2-422.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

CYGDR0010w.
SGDS000029654. FLP1.

Phylogenomic databases

eggNOGNOG255844.
OrthoDBEOG7TXKS7.

Gene expression databases

GenevestigatorP03870.

Family and domain databases

Gene3D1.10.443.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat-core.
IPR010998. Integrase_Lambda-type_N.
IPR005626. Recombinase_Flp.
IPR022647. Recombinase_Flp_N.
[Graphical view]
PfamPF05202. Flp_C. 1 hit.
PF03930. Flp_N. 1 hit.
[Graphical view]
SUPFAMSSF47823. SSF47823. 1 hit.
SSF56349. SSF56349. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP03870.

Entry information

Entry nameFLP_YEAST
AccessionPrimary (citable) accession number: P03870
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 13, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references