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P03870

- FLP_YEAST

UniProt

P03870 - FLP_YEAST

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Protein

Site-specific recombinase Flp

Gene
FLP1, R0010W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei343 – 3431O-(3'-phospho-DNA)-tyrosine intermediate1 Publication

GO - Molecular functioni

  1. DNA binding, bending Source: SGD
  2. double-stranded DNA binding Source: SGD
  3. single-stranded DNA binding Source: SGD
  4. site-specific recombinase activity Source: SGD

GO - Biological processi

  1. DNA integration Source: UniProtKB-KW
  2. plasmid recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA integration, DNA recombination

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Site-specific recombinase Flp
Short name:
FLP
Alternative name(s):
Protein Able
Gene namesi
Name:FLP1
Ordered Locus Names:R0010W
Encoded oniPlasmid 2-micron0 Publication
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Plasmid 2-micron

Organism-specific databases

CYGDiR0010w.
SGDiS000029654. FLP1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi305 – 3051H → L or P: Inactive and weakened DNA binding. 1 Publication
Mutagenesisi305 – 3051H → Q: Reduced activity. 1 Publication
Mutagenesisi308 – 3081R → G: Inactive and weakened DNA binding. 1 Publication
Mutagenesisi343 – 3431Y → F or S: No strand cleavage or recombination. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Site-specific recombinase FlpPRO_0000197567Add
BLAST

Expressioni

Inductioni

Repressed by the negative regulatory complex REP1-REP2.1 Publication

Gene expression databases

GenevestigatoriP03870.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1
423
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 97
Helixi12 – 2413
Helixi28 – 314
Helixi32 – 343
Helixi35 – 4612
Turni47 – 493
Helixi54 – 6512
Beta strandi69 – 713
Turni72 – 754
Beta strandi76 – 805
Helixi86 – 9611
Beta strandi101 – 1055
Helixi116 – 12813
Helixi138 – 14912
Beta strandi150 – 1523
Helixi154 – 16310
Turni164 – 1685
Helixi172 – 18817
Helixi192 – 1965
Beta strandi199 – 2068
Turni209 – 2113
Beta strandi214 – 2196
Beta strandi228 – 2325
Beta strandi236 – 2383
Helixi241 – 25111
Beta strandi267 – 2693
Beta strandi272 – 2754
Helixi279 – 29012
Helixi294 – 2974
Helixi306 – 31712
Helixi324 – 3285
Helixi337 – 3404
Turni341 – 3433
Helixi352 – 3587
Beta strandi361 – 3655
Turni366 – 3694
Beta strandi370 – 3734
Helixi380 – 38910
Helixi395 – 3995
Helixi401 – 4033
Turni404 – 4063
Helixi409 – 42012

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FLOX-ray2.65A/B/C/D2-423[»]
1M6XX-ray2.80A/B/C/D1-423[»]
1P4EX-ray2.70A/B2-423[»]
C/D2-422[»]
ProteinModelPortaliP03870.
SMRiP03870. Positions 2-422.

Miscellaneous databases

EvolutionaryTraceiP03870.

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.

Phylogenomic databases

eggNOGiNOG255844.
OrthoDBiEOG7TXKS7.

Family and domain databases

Gene3Di1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat-core.
IPR010998. Integrase_Lambda-type_N.
IPR005626. Recombinase_Flp.
IPR022647. Recombinase_Flp_N.
[Graphical view]
PfamiPF05202. Flp_C. 1 hit.
PF03930. Flp_N. 1 hit.
[Graphical view]
SUPFAMiSSF47823. SSF47823. 1 hit.
SSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

P03870-1 [UniParc]FASTAAdd to Basket

« Hide

MPQFGILCKT PPKVLVRQFV ERFERPSGEK IALCAAELTY LCWMITHNGT    50
AIKRATFMSY NTIISNSLSF DIVNKSLQFK YKTQKATILE ASLKKLIPAW 100
EFTIIPYYGQ KHQSDITDIV SSLQLQFESS EEADKGNSHS KKMLKALLSE 150
GESIWEITEK ILNSFEYTSR FTKTKTLYQF LFLATFINCG RFSDIKNVDP 200
KSFKLVQNKY LGVIIQCLVT ETKTSVSRHI YFFSARGRID PLVYLDEFLR 250
NSEPVLKRVN RTGNSSSNKQ EYQLLKDNLV RSYNKALKKN APYSIFAIKN 300
GPKSHIGRHL MTSFLSMKGL TELTNVVGNW SDKRASAVAR TTYTHQITAI 350
PDHYFALVSR YYAYDPISKE MIALKDETNP IEEWQHIEQL KGSAEGSIRY 400
PAWNGIISQE VLDYLSSYIN RRI 423
Length:423
Mass (Da):48,620
Last modified:July 21, 1986 - v1
Checksum:iB9C3DE95AB253BC3
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51G → D AA sequence 1 Publication
Sequence conflicti5 – 51G → D AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01347 Genomic DNA. Translation: AAB59340.1.
PIRiA04502. PDBYA.
RefSeqiNP_040488.1. NC_001398.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01347 Genomic DNA. Translation: AAB59340.1 .
PIRi A04502. PDBYA.
RefSeqi NP_040488.1. NC_001398.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FLO X-ray 2.65 A/B/C/D 2-423 [» ]
1M6X X-ray 2.80 A/B/C/D 1-423 [» ]
1P4E X-ray 2.70 A/B 2-423 [» ]
C/D 2-422 [» ]
ProteinModelPortali P03870.
SMRi P03870. Positions 2-422.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

CYGDi R0010w.
SGDi S000029654. FLP1.

Phylogenomic databases

eggNOGi NOG255844.
OrthoDBi EOG7TXKS7.

Miscellaneous databases

EvolutionaryTracei P03870.

Gene expression databases

Genevestigatori P03870.

Family and domain databases

Gene3Di 1.10.443.10. 1 hit.
InterProi IPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat-core.
IPR010998. Integrase_Lambda-type_N.
IPR005626. Recombinase_Flp.
IPR022647. Recombinase_Flp_N.
[Graphical view ]
Pfami PF05202. Flp_C. 1 hit.
PF03930. Flp_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47823. SSF47823. 1 hit.
SSF56349. SSF56349. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the yeast plasmid."
    Hartley J.L., Donelson J.E.
    Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A364A D5.
  2. "The FLP protein of the 2-micron plasmid of yeast. Purification of the protein from Escherichia coli cells expressing the cloned FLP gene."
    Babineau D., Vetter D., Andrews B.J., Gronostajski R.M., Proteau G.A., Beatty L.G., Sadowski P.D.
    J. Biol. Chem. 260:12313-12319(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-12.
  3. "Identification of the DNA-binding domain of the FLP recombinase."
    Pan H., Clary D., Sadowski P.D.
    J. Biol. Chem. 266:11347-11354(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-7; 124-129 AND 148-153, FUNCTION.
  4. "Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 micrometer plasmid."
    Hindley J., Phear G.A.
    Nucleic Acids Res. 7:361-375(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 250-423.
  5. "Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
    Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
    EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae."
    Reynolds A.E., Murray A.W.H., Szostak J.W.
    Mol. Cell. Biol. 7:3566-3573(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Mutations in the 2-microns circle site-specific recombinase that abolish recombination without affecting substrate recognition."
    Prasad P.V., Young L.J., Jayaram M.
    Proc. Natl. Acad. Sci. U.S.A. 84:2189-2193(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF TYR-343.
  8. "Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination."
    Parsons R.L., Prasad P.V., Harshey R.M., Jayaram M.
    Mol. Cell. Biol. 8:3303-3310(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-305 AND ARG-308.
  9. "FLP protein of 2 mu circle plasmid of yeast induces multiple bends in the FLP recognition target site."
    Schwartz C.J., Sadowski P.D.
    J. Mol. Biol. 216:289-298(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Identification of the active site tyrosine of Flp recombinase. Possible relevance of its location to the mechanism of recombination."
    Evans B.R., Chen J.W., Parsons R.L., Bauer T.K., Teplow D.B., Jayaram M.
    J. Biol. Chem. 265:18504-18510(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITE.
  11. "Crystal structure of a Flp recombinase-Holliday junction complex: assembly of an active oligomer by helix swapping."
    Chen Y., Narendra U., Iype L.E., Cox M.M., Rice P.A.
    Mol. Cell 6:885-897(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) IN COMPLEX WITH DNA, SUBUNIT.

Entry informationi

Entry nameiFLP_YEAST
AccessioniPrimary (citable) accession number: P03870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

External Data

Dasty 3

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