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Protein

Site-specific recombinase Flp

Gene

FLP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei343O-(3'-phospho-DNA)-tyrosine intermediate1 Publication1

GO - Molecular functioni

  • DNA binding, bending Source: SGD
  • double-stranded DNA binding Source: SGD
  • single-stranded DNA binding Source: SGD
  • site-specific recombinase activity Source: SGD

GO - Biological processi

  • DNA integration Source: UniProtKB-KW
  • plasmid recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA integration, DNA recombination

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Site-specific recombinase Flp
Short name:
FLP
Alternative name(s):
Protein Able
Gene namesi
Name:FLP1
Ordered Locus Names:R0010W
Encoded oniPlasmid 2-micron0 Publication
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Plasmid 2-micron

Organism-specific databases

SGDiS000029654. FLP1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi305H → L or P: Inactive and weakened DNA binding. 1 Publication1
Mutagenesisi305H → Q: Reduced activity. 1 Publication1
Mutagenesisi308R → G: Inactive and weakened DNA binding. 1 Publication1
Mutagenesisi343Y → F or S: No strand cleavage or recombination. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975671 – 423Site-specific recombinase FlpAdd BLAST423

Proteomic databases

PRIDEiP03870.

Expressioni

Inductioni

Repressed by the negative regulatory complex REP1-REP2.1 Publication

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1423
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 9Combined sources7
Helixi12 – 24Combined sources13
Helixi28 – 31Combined sources4
Helixi32 – 34Combined sources3
Helixi35 – 46Combined sources12
Turni47 – 49Combined sources3
Helixi54 – 65Combined sources12
Beta strandi69 – 71Combined sources3
Turni72 – 75Combined sources4
Beta strandi76 – 80Combined sources5
Helixi86 – 96Combined sources11
Beta strandi101 – 105Combined sources5
Helixi116 – 128Combined sources13
Helixi138 – 149Combined sources12
Beta strandi150 – 152Combined sources3
Helixi154 – 163Combined sources10
Turni164 – 168Combined sources5
Helixi172 – 188Combined sources17
Helixi192 – 196Combined sources5
Beta strandi199 – 206Combined sources8
Turni209 – 211Combined sources3
Beta strandi214 – 219Combined sources6
Beta strandi228 – 232Combined sources5
Beta strandi236 – 238Combined sources3
Helixi241 – 251Combined sources11
Beta strandi267 – 269Combined sources3
Beta strandi272 – 275Combined sources4
Helixi279 – 290Combined sources12
Helixi294 – 297Combined sources4
Helixi306 – 317Combined sources12
Helixi324 – 328Combined sources5
Helixi337 – 340Combined sources4
Turni341 – 343Combined sources3
Helixi352 – 358Combined sources7
Beta strandi361 – 365Combined sources5
Turni366 – 369Combined sources4
Beta strandi370 – 373Combined sources4
Helixi380 – 389Combined sources10
Helixi395 – 399Combined sources5
Helixi401 – 403Combined sources3
Turni404 – 406Combined sources3
Helixi409 – 420Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FLOX-ray2.65A/B/C/D2-423[»]
1M6XX-ray2.80A/B/C/D1-423[»]
1P4EX-ray2.70A/B2-423[»]
C/D2-422[»]
ProteinModelPortaliP03870.
SMRiP03870.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03870.

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Phylogenomic databases

InParanoidiP03870.
OrthoDBiEOG092C2Q6Y.

Family and domain databases

Gene3Di1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR010998. Integrase_Lambda-type_N.
IPR005626. Recombinase_Flp.
IPR022647. Recombinase_Flp_N.
[Graphical view]
PfamiPF05202. Flp_C. 1 hit.
PF03930. Flp_N. 1 hit.
[Graphical view]
SUPFAMiSSF47823. SSF47823. 1 hit.
SSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

P03870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQFGILCKT PPKVLVRQFV ERFERPSGEK IALCAAELTY LCWMITHNGT
60 70 80 90 100
AIKRATFMSY NTIISNSLSF DIVNKSLQFK YKTQKATILE ASLKKLIPAW
110 120 130 140 150
EFTIIPYYGQ KHQSDITDIV SSLQLQFESS EEADKGNSHS KKMLKALLSE
160 170 180 190 200
GESIWEITEK ILNSFEYTSR FTKTKTLYQF LFLATFINCG RFSDIKNVDP
210 220 230 240 250
KSFKLVQNKY LGVIIQCLVT ETKTSVSRHI YFFSARGRID PLVYLDEFLR
260 270 280 290 300
NSEPVLKRVN RTGNSSSNKQ EYQLLKDNLV RSYNKALKKN APYSIFAIKN
310 320 330 340 350
GPKSHIGRHL MTSFLSMKGL TELTNVVGNW SDKRASAVAR TTYTHQITAI
360 370 380 390 400
PDHYFALVSR YYAYDPISKE MIALKDETNP IEEWQHIEQL KGSAEGSIRY
410 420
PAWNGIISQE VLDYLSSYIN RRI
Length:423
Mass (Da):48,620
Last modified:July 21, 1986 - v1
Checksum:iB9C3DE95AB253BC3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5G → D AA sequence (PubMed:2995370).Curated1
Sequence conflicti5G → D AA sequence (PubMed:2040639).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01347 Genomic DNA. Translation: AAB59340.1.
PIRiA04502. PDBYA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01347 Genomic DNA. Translation: AAB59340.1.
PIRiA04502. PDBYA.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FLOX-ray2.65A/B/C/D2-423[»]
1M6XX-ray2.80A/B/C/D1-423[»]
1P4EX-ray2.70A/B2-423[»]
C/D2-422[»]
ProteinModelPortaliP03870.
SMRiP03870.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP03870.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

SGDiS000029654. FLP1.

Phylogenomic databases

InParanoidiP03870.
OrthoDBiEOG092C2Q6Y.

Miscellaneous databases

EvolutionaryTraceiP03870.
PROiP03870.

Family and domain databases

Gene3Di1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR010998. Integrase_Lambda-type_N.
IPR005626. Recombinase_Flp.
IPR022647. Recombinase_Flp_N.
[Graphical view]
PfamiPF05202. Flp_C. 1 hit.
PF03930. Flp_N. 1 hit.
[Graphical view]
SUPFAMiSSF47823. SSF47823. 1 hit.
SSF56349. SSF56349. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFLP_YEAST
AccessioniPrimary (citable) accession number: P03870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.