P03835 (INSG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transposase insG for insertion sequence element IS4

Gene names

Name:	insG
Ordered Locus Names:	b4278, JW5767

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the transposition of the insertion sequence IS4.
Sequence similarities	Belongs to the transposase 11 family.

Ontologies

Keywords
Biological process	DNA recombination Transposition
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome Transposable element
Gene Ontology (GO)
Biological process	transposition, DNA-mediated Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transposase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Transposase insG for insertion sequence element IS4		PRO_0000173291

Sequences

	Sequence		Length	Mass (Da)
	P03835 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 0B9CAAA0FFAC724E	FASTA	442	50,386
	10 20 30 40 50 60 MHIGQALDLV SRYDSLRNPL TSLGDYLDPE LISRCLAESG TVTLRKRRLP LEMMVWCIVG 70 80 90 100 110 120 MALERKEPLH QIVNRLDIML PGNRPFVAPS AVIQARQRLG SEAVRRVFTK TAQLWHNATP 130 140 150 160 170 180 HPHWCGLTLL AIDGVFWRTP DTPENDAAFP RQTHAGNPAL YPQVKMVCQM ELTSHLLTAA 190 200 210 220 230 240 AFGTMKNSEN ELAEQLIEQT GDNTLTLMDK GYYSLGLLNA WSLAGEHRHW MIPLRKGAQY 250 260 270 280 290 300 EEIRKLGKGD HLVKLKTSPQ ARKKWPGLGN EVTARLLTVT RKGKVCHLLT SMTDAMRFPG 310 320 330 340 350 360 GEMGDLYSHR WEIELGYREI KQTMQRSRLT LRSKKPELVE QELWGVLLAY NLVRYQMIKM 370 380 390 400 410 420 AEHLKGYWPN QLSFSESCGM VMRMLMTLQG ASPGRIPELM RDLASMGQLV KLPTRRERAF 430 440 PRVVKERPWK YPTAPKKSQS VA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of IS4." Klaer R., Kuhn S., Tillmann E., Fritz H.-J., Starlinger P. Mol. Gen. Genet. 181:169-175(1981) [PubMed: 6268937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01733 Genomic DNA. No translation available. U14003 Genomic DNA. Translation: AAA97174.1. U00096 Genomic DNA. Translation: AAC77234.1. AP009048 Genomic DNA. Translation: BAE78272.1.
PIR	IEEC41. A04463.
RefSeq	NP_418698.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P03835.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002793; EBESCP00000002793; EBESCG00000002279. EBESCT00000015973; EBESCP00000015264; EBESCG00000015033.
GeneID	948805.
GenomeReviews	Gene locus JW5767 in contig AP009048_GR. Gene locus b4278 in contig U00096_GR.
KEGG	ecj:JW5767. eco:b4278.
PATRIC	32124129. VBIEscCol129921_4408.
Organism-specific databases
EchoBASE	EB4735.
EcoGene	EG40007. insG.
Phylogenomic databases
eggNOG	COG3385.
GeneTree	EBGT00050000008395.
HOGENOM	HBG753189.
OMA	CGTHAIV.
PhylomeDB	P03835.
ProtClustDB	CLSK504976.
Enzyme and pathway databases
BioCyc	EcoCyc:G7900-MONOMER.
Gene expression databases
Genevestigator	P03835.
Family and domain databases
InterPro	IPR012337. RNaseH-like_dom. IPR002559. Transposase_11. IPR024473. Transposases_IS4_N. [Graphical view]
KO	K07495.
Pfam	PF01609. DDE_Tnp_1. 1 hit. PF13006. Nterm_IS4. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

INSG_ECOLI

Accession

Primary (citable) accession number: P03835
Secondary accession number(s): Q2M634

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program