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Protein

Putative general secretion pathway protein B

Gene

gspB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-binding protein that may be required for the initiation of chromosome replication. May play a regulatory role under conditions of derepressed gsp gene expression.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciEcoCyc:EG11263-MONOMER.
ECOL316407:JW3284-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative general secretion pathway protein B
Alternative name(s):
Protein PinO
Protein PioO
Gene namesi
Name:gspB
Synonyms:pinO, pioO, pno
Ordered Locus Names:b3322, JW3284
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11263. gspB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei28 – 4821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 139139Putative general secretion pathway protein BPRO_0000058445Add
BLAST

Proteomic databases

PaxDbiP03825.
PRIDEiP03825.

Expressioni

Inductioni

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261294. 169 interactions.
STRINGi511145.b3322.

Structurei

3D structure databases

ProteinModelPortaliP03825.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107ACA. Bacteria.
ENOG410Z2ZU. LUCA.
HOGENOMiHOG000009789.
KOiK02451.
OMAiQSMWHER.

Sequencei

Sequence statusi: Complete.

P03825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEFYIAARE QKETGHPGIF SRQKHSTIIY VICLLLICLW FAGMVLVGGY
60 70 80 90 100
ARQLWVLWIV KAEVTVEAET PAFKQSTQHY FFKKQPLPVV ESVEEEDDPG
110 120 130
VAVENAPSSS EDEENTVEES EEKAGLRERV KNALNELER
Length:139
Mass (Da):15,876
Last modified:November 1, 1995 - v2
Checksum:i36DE3301CB52EE47
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 6666MFEFY…AEVTV → MTDLPVVCRNGAGWWVCQAA MGALDSKSRSHL in V00344 (PubMed:7037196).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00344 Genomic DNA. No translation available.
U18997 Genomic DNA. Translation: AAA58119.1.
U00096 Genomic DNA. Translation: AAC76347.1.
AP009048 Genomic DNA. Translation: BAE77969.1.
PIRiE65125. QQECRP.
RefSeqiNP_417781.1. NC_000913.3.
WP_000461450.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76347; AAC76347; b3322.
BAE77969; BAE77969; BAE77969.
GeneIDi947826.
KEGGiecj:JW3284.
eco:b3322.
PATRICi32122076. VBIEscCol129921_3415.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00344 Genomic DNA. No translation available.
U18997 Genomic DNA. Translation: AAA58119.1.
U00096 Genomic DNA. Translation: AAC76347.1.
AP009048 Genomic DNA. Translation: BAE77969.1.
PIRiE65125. QQECRP.
RefSeqiNP_417781.1. NC_000913.3.
WP_000461450.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP03825.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261294. 169 interactions.
STRINGi511145.b3322.

Proteomic databases

PaxDbiP03825.
PRIDEiP03825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76347; AAC76347; b3322.
BAE77969; BAE77969; BAE77969.
GeneIDi947826.
KEGGiecj:JW3284.
eco:b3322.
PATRICi32122076. VBIEscCol129921_3415.

Organism-specific databases

EchoBASEiEB1243.
EcoGeneiEG11263. gspB.

Phylogenomic databases

eggNOGiENOG4107ACA. Bacteria.
ENOG410Z2ZU. LUCA.
HOGENOMiHOG000009789.
KOiK02451.
OMAiQSMWHER.

Enzyme and pathway databases

BioCyciEcoCyc:EG11263-MONOMER.
ECOL316407:JW3284-MONOMER.

Miscellaneous databases

PROiP03825.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiGSPB_ECOLI
AccessioniPrimary (citable) accession number: P03825
Secondary accession number(s): Q2M6Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.