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P03819 (KEFC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione-regulated potassium-efflux system protein kefC
Alternative name(s):
K(+)/H(+) antiporter
Gene names
Name:kefC
Synonyms:trkC
Ordered Locus Names:b0047, JW0046
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length620 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport. HAMAP MF_01413

Subcellular location

Cell inner membrane; Multi-pass membrane protein HAMAP MF_01413.

Miscellaneous

KefC system is apparently under negative feedback regulation by glutathione and removal of glutathione leads to partial activation of the system. Complete activation requires the formation of glutathione metabolites that carry a hydrophobic substituent on the S-atom. HAMAP MF_01413

Sequence similarities

Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. KefC subfamily. [View classification]

Contains 1 RCK N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 620620Glutathione-regulated potassium-efflux system protein kefC HAMAP MF_01413
PRO_0000196606

Regions

Topological domain1 – 33Periplasmic Potential
Transmembrane4 – 2421Helical; Potential
Topological domain251Cytoplasmic Potential
Transmembrane26 – 4621Helical; Potential
Topological domain47 – 537Periplasmic Potential
Transmembrane54 – 7421Helical; Potential
Topological domain75 – 8915Cytoplasmic Potential
Transmembrane90 – 11021Helical; Potential
Topological domain111 – 1133Periplasmic Potential
Transmembrane114 – 13421Helical; Potential
Topological domain135 – 14814Cytoplasmic Potential
Transmembrane149 – 16921Helical; Potential
Topological domain170 – 1778Periplasmic Potential
Transmembrane178 – 19821Helical; Potential
Topological domain199 – 21315Cytoplasmic Potential
Transmembrane214 – 23320Helical; Potential
Topological domain234 – 2363Periplasmic Potential
Transmembrane237 – 25418Helical; Potential
Topological domain255 – 26915Cytoplasmic Potential
Transmembrane270 – 29021Helical; Potential
Topological domain291 – 2933Periplasmic Potential
Transmembrane294 – 31421Helical; Potential
Topological domain315 – 32612Cytoplasmic Potential
Transmembrane327 – 34721Helical; Potential
Topological domain348 – 35811Periplasmic Potential
Transmembrane359 – 37921Helical; Potential
Topological domain380 – 620241Cytoplasmic Potential
Domain401 – 523123RCK N-terminal

Secondary structure

1 ...................... 620
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P03819 [UniParc].

Last modified November 1, 1991. Version 2.
Checksum: 9995B2E8E3C1DCE3

FASTA62067,796
        10         20         30         40         50         60 
MDSHTLIQAL IYLGSAALIV PIAVRLGLGS VLGYLIAGCI IGPWGLRLVT DAESILHFAE 

        70         80         90        100        110        120 
IGVVLMLFII GLELDPQRLW KLRAAVFGCG ALQMVICGGL LGLFCMLLGL RWQVAELIGM 

       130        140        150        160        170        180 
TLALSSTAIA MQAMNERNLM VTQMGRSAFA VLLFQDIAAI PLVAMIPLLA TSSASTTMGA 

       190        200        210        220        230        240 
FALSALKVAG ALVLVVLLGR YVTRPALRFV ARSGLREVFS AVALFLVFGF GLLLEEVGLS 

       250        260        270        280        290        300 
MAMGAFLAGV LLASSEYRHA LESDIEPFKG LLLGLFFIGV GMSIDFGTLL ENPLRIVILL 

       310        320        330        340        350        360 
LGFLIIKIAM LWLIARPLQV PNKQRRWFAV LLGQGSEFAF VVFGAAQMAN VLEPEWAKSL 

       370        380        390        400        410        420 
TLAVALSMAA TPILLVILNR LEQSSTEEAR EADEIDEEQP RVIIAGFGRF GQITGRLLLS 

       430        440        450        460        470        480 
SGVKMVVLDH DPDHIETLRK FGMKVFYGDA TRMDLLESAG AAKAEVLINA IDDPQTNLQL 

       490        500        510        520        530        540 
TEMVKEHFPH LQIIARARDV DHYIRLRQAG VEKPERETFE GALKTGRLAL ESLGLGPYEA 

       550        560        570        580        590        600 
RERADVFRRF NIQMVEEMAM VENDTKARAA VYKRTSAMLS EIITEDREHL SLIQRHGWQG 

       610        620 
TEEGKHTGNM ADEPETKPSS

« Hide

References

« Hide 'large scale' references
[1]"The cloning and DNA sequence of the gene for the glutathione-regulated potassium-efflux system KefC of Escherichia coli."
Munro A.W., Ritchie G.Y., Lamb A.J., Douglas R.M., Booth I.R.
Mol. Microbiol. 5:607-616(1991) [PubMed: 2046548] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / CS520.
[2]"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Nucleotide sequence of the E coli gene coding for dihydrofolate reductase."
Smith D.R., Calvo J.M.
Nucleic Acids Res. 8:2255-2274(1980) [PubMed: 6159575] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 500-620.
Strain: K12.
[6]"The putative Na+/H+ antiporter (NapA) of Enterococcus hirae is homologous to the putative K+/H+ antiporter (KefC) of Escherichia coli."
Reizer J., Reizer A., Saier M.H. Jr.
FEMS Microbiol. Lett. 73:161-163(1992) [PubMed: 1325937] [Abstract]
Cited for: SIMILARITY TO NAPA.
[7]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X56742 Genomic DNA. Translation: CAA40066.1.
U00096 Genomic DNA. Translation: AAC73158.1.
AP009048 Genomic DNA. Translation: BAB96615.1.
J01609 Genomic DNA. No translation available.
PIRQQECRD. S40568.
RefSeqNP_414589.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3EYWX-ray2.40A/B401-620[»]
3L9WX-ray1.75A/B401-620[»]
3L9XX-ray2.10A/B401-620[»]
ProteinModelPortalP03819.
SMRP03819. Positions 401-583.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10072N.
IntActP03819. 2 interactions.
MINTMINT-1257196.

Protein family/group databases

TCDB2.A.37.1.1. monovalent cation:proton antiporter-2 (CPA2) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000004398; EBESCP00000004398; EBESCG00000003586.
EBESCT00000017034; EBESCP00000016325; EBESCG00000016093.
GeneID944773.
GenomeReviewsGene locus JW0046 in contig AP009048_GR.
Gene locus b0047 in contig U00096_GR.
KEGGecj:JW0046.
eco:b0047.
PATRIC32115193. VBIEscCol129921_0048.

Organism-specific databases

EchoBASEEB0516.
EcoGeneEG10521. kefC.

Phylogenomic databases

eggNOGCOG0475.
GeneTreeEBGT00050000011199.
HOGENOMHBG428809.
OMADIMHFAE.
PhylomeDBP03819.
ProtClustDBPRK03562.

Enzyme and pathway databases

BioCycEcoCyc:KEFC-MONOMER.

Gene expression databases

GenevestigatorP03819.

Family and domain databases

HAMAPMF_01413. K-H-efflux_KefC.
[Tree]
InterProIPR006153. Cation/H_exchanger.
IPR004771. K/H_exchanger.
IPR023941. K_H_efflux_KefC.
IPR006036. K_uptake_TrkA.
IPR016040. NAD(P)-bd_dom.
IPR003148. RCK_N.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK11745.
PfamPF00999. Na_H_Exchanger. 1 hit.
PF02254. TrkA_N. 1 hit.
[Graphical view]
PRINTSPR00335. KUPTAKETRKA.
TIGRFAMsTIGR00932. 2a37. 1 hit.
PROSITEPS51201. RCK_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKEFC_ECOLI
AccessionPrimary (citable) accession number: P03819
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1991
Last modified: January 25, 2012
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents