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Protein

Spanin, inner membrane subunit

Gene

18.5

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Component of the spanin complex that disrupts the host outer membrane and participates in cell lysis during virus exit. The spanin complex conducts the final step in host lysis by disrupting the outer membrane after holin and endolysin action have permeabilized the inner membrane and degraded the host peptidoglycans. Host outer membrane disruption is possibly due to local fusion between the inner and outer membrane performed by the spanin complex.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease
Biological processCytolysis, Host cell lysis by virus, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Spanin, inner membrane subunit1 Publication
Short name:
i-spanin
Alternative name(s):
Gene product 18.5
Short name:
Gp18.5
Gene namesi
Ordered Locus Names:18.5
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7Cytoplasmic1 Publication7
Transmembranei8 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini25 – 143Periplasmic1 PublicationAdd BLAST119

GO - Cellular componenti

Keywords - Cellular componenti

Host cell inner membrane, Host cell membrane, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001065371 – 143Spanin, inner membrane subunitAdd BLAST143

Interactioni

Subunit structurei

Interacts (via C-terminus) with the spanin outer lipoprotein subunit (o-spanin) (via C-terminus). Part of the spanin complex which spans the entire periplasmic space. The spanin complex is composed of spanin inner membrane subunit and spanin outer membrane subunit.1 Publication

Protein-protein interaction databases

IntActiP03803. 1 interactor.
MINTiMINT-1513615.

Family & Domainsi

Sequence similaritiesi

Belongs to the T7likevirus i-spanin family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG0900010C.

Family and domain databases

InterProiView protein in InterPro
IPR004929. i-spanin.
IPR016417. i-spanin_T7likevirus.
PfamiView protein in Pfam
PF03245. Phage_lysis. 1 hit.
PIRSFiPIRSF004485. T7_18-5_prd. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD033200. Phge_lysis-endo-Pept_T7/18-5. 1 hit.

Sequencei

Sequence statusi: Complete.

P03803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEFLRKLIP WVLAGMLFGL GWHLGSDSMD AKWKQEVHNE YVKRVEAAKS
60 70 80 90 100
TQRAIDAVSA KYQEDLAALE GSTDRIISDL RSDNKRLRVR VKTTGTSDGQ
110 120 130 140
CGFEPDGRAE LDDRDAKRIL AVTQKGDAWI RALQDTIREL QRK
Length:143
Mass (Da):16,243
Last modified:July 21, 1986 - v1
Checksum:i7A2634B7C4C16AEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24438.1.
PIRiA04428. Q8BPE7.
RefSeqiNP_042008.1. NC_001604.1.

Genome annotation databases

GeneIDi1261067.
KEGGivg:1261067.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24438.1.
PIRiA04428. Q8BPE7.
RefSeqiNP_042008.1. NC_001604.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03803. 1 interactor.
MINTiMINT-1513615.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261067.
KEGGivg:1261067.

Phylogenomic databases

OrthoDBiVOG0900010C.

Family and domain databases

InterProiView protein in InterPro
IPR004929. i-spanin.
IPR016417. i-spanin_T7likevirus.
PfamiView protein in Pfam
PF03245. Phage_lysis. 1 hit.
PIRSFiPIRSF004485. T7_18-5_prd. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD033200. Phge_lysis-endo-Pept_T7/18-5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPAN1_BPT7
AccessioniPrimary (citable) accession number: P03803
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 15, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.