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Protein

Holin

Gene

17.5

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Participates in determining the precise timing of host cell lysis. Participates with the endolysin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell.UniRule annotation2 Publications

GO - Biological processi

Keywordsi

Biological processCytolysis, Host cell lysis by virus, Virus exit from host cell

Protein family/group databases

TCDBi1.E.6.1.1. the t7 holin (t7 holin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
HolinUniRule annotation
Alternative name(s):
Gene product 17.5
Short name:
Gp17.5
Gene namesi
Ordered Locus Names:17.5
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

Subcellular locationi

  • Host cell inner membrane UniRule annotation1 Publication; Single-pass type II membrane protein UniRule annotation1 Publication; Periplasmic side UniRule annotation1 Publication

  • Note: Classified as a class II holin although it seems to have only one transmembrane domain.1 PublicationUniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36CytoplasmicUniRule annotationAdd BLAST36
Transmembranei37 – 55Helical; Signal-anchor for type II membrane proteinUniRule annotationAdd BLAST19
Topological domaini56 – 67PeriplasmicUniRule annotationAdd BLAST12

GO - Cellular componenti

Keywords - Cellular componenti

Host cell inner membrane, Host cell membrane, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001065331 – 67HolinAdd BLAST67

Interactioni

Subunit structurei

Homomultimer.UniRule annotation1 Publication

Structurei

3D structure databases

SMRiP03802.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the T7likevirus holin family.UniRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090001UY.

Family and domain databases

HAMAPiMF_04108. HOLIN_T7. 1 hit.
InterProiView protein in InterPro
IPR019682. Phage_T7_Gp17.5_holin.
PfamiView protein in Pfam
PF10746. Phage_holin_2_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P03802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLDFNNEL IKAAPIVGTG VADVSARLFF GLSLNEWFYV AAIAYTVVQI
60
GAKVVDKMID WKKANKE
Length:67
Mass (Da):7,392
Last modified:July 21, 1986 - v1
Checksum:iFEA1040A783F4AC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24436.1.
PIRiA04427. Q7BPE7.
RefSeqiNP_042006.1. NC_001604.1.

Genome annotation databases

GeneIDi1261022.
KEGGivg:1261022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24436.1.
PIRiA04427. Q7BPE7.
RefSeqiNP_042006.1. NC_001604.1.

3D structure databases

SMRiP03802.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.E.6.1.1. the t7 holin (t7 holin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261022.
KEGGivg:1261022.

Phylogenomic databases

OrthoDBiVOG090001UY.

Family and domain databases

HAMAPiMF_04108. HOLIN_T7. 1 hit.
InterProiView protein in InterPro
IPR019682. Phage_T7_Gp17.5_holin.
PfamiView protein in Pfam
PF10746. Phage_holin_2_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHOLIN_BPT7
AccessioniPrimary (citable) accession number: P03802
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 10, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.