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P03772

- PP_LAMBD

UniProt

P03772 - PP_LAMBD

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Protein
Serine/threonine-protein phosphatase
Gene
N/A
Organism
Enterobacteria phage lambda (Bacteriophage lambda)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi20 – 201Manganese 1
Metal bindingi22 – 221Manganese 1
Metal bindingi49 – 491Manganese 1
Metal bindingi49 – 491Manganese 2
Metal bindingi75 – 751Manganese 2
Active sitei76 – 761Proton donor By similarity
Metal bindingi186 – 1861Manganese 2

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
  3. protein binding Source: IntAct
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase (EC:3.1.3.16)
OrganismiEnterobacteria phage lambda (Bacteriophage lambda)
Taxonomic identifieri10710 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000001711: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221Serine/threonine-protein phosphatase
PRO_0000058912Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Plk4Q647022EBI-4478820,EBI-2552433From a different organism.

Protein-protein interaction databases

DIPiDIP-44028N.
IntActiP03772. 3 interactions.
MINTiMINT-4053809.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86
Helixi9 – 113
Beta strandi15 – 184
Helixi25 – 3511
Turni39 – 413
Beta strandi43 – 464
Beta strandi51 – 555
Helixi57 – 615
Helixi62 – 654
Beta strandi69 – 713
Helixi75 – 8410
Helixi91 – 944
Turni95 – 973
Helixi98 – 1036
Helixi106 – 11914
Beta strandi124 – 1307
Beta strandi133 – 1375
Beta strandi144 – 1463
Helixi155 – 1606
Helixi163 – 1697
Beta strandi179 – 1846
Beta strandi193 – 1953
Beta strandi198 – 2003
Helixi205 – 2084
Beta strandi213 – 2175

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G5BX-ray2.15A/B/C1-221[»]
ProteinModelPortaliP03772.
SMRiP03772. Positions 1-218.

Miscellaneous databases

EvolutionaryTraceiP03772.

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03772-1 [UniParc]FASTAAdd to Basket

« Hide

MRYYEKIDGS KYRNIWVVGD LHGCYTNLMN KLDTIGFDNK KDLLISVGDL    50
VDRGAENVEC LELITFPWFR AVRGNHEQMM IDGLSERGNV NHWLLNGGGW 100
FFNLDYDKEI LAKALAHKAD ELPLIIELVS KDKKYVICHA DYPFDEYEFG 150
KPVDHQQVIW NRERISNSQN GIVKEIKGAD TFIFGHTPAV KPLKFANQMY 200
IDTGAVFCGN LTLIQVQGEG A 221
Length:221
Mass (Da):25,219
Last modified:July 21, 1986 - v1
Checksum:i5CE0E1A0F3BC5CB5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02459 Genomic DNA. Translation: AAA96594.1.
PIRiG43011. Q1BP1L.
RefSeqiNP_040641.1. NC_001416.1.

Genome annotation databases

GeneIDi2703476.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02459 Genomic DNA. Translation: AAA96594.1 .
PIRi G43011. Q1BP1L.
RefSeqi NP_040641.1. NC_001416.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G5B X-ray 2.15 A/B/C 1-221 [» ]
ProteinModelPortali P03772.
SMRi P03772. Positions 1-218.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-44028N.
IntActi P03772. 3 interactions.
MINTi MINT-4053809.

Chemistry

BindingDBi P03772.
ChEMBLi CHEMBL3695.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2703476.

Miscellaneous databases

EvolutionaryTracei P03772.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Discovery of a protein phosphatase activity encoded in the genome of bacteriophage lambda. Probable identity with open reading frame 221."
    Cohen P.T.W., Cohen P.
    Biochem. J. 260:931-934(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Structure of the bacteriophage lambda Ser/Thr protein phosphatase with sulfate ion bound in two coordination modes."
    Voegtli W.C., White D.J., Reiter N.J., Rusnak F., Rosenzweig A.C.
    Biochemistry 39:15365-15374(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) IN COMPLEX WITH MANGANESE IONS, COFACTOR.

Entry informationi

Entry nameiPP_LAMBD
AccessioniPrimary (citable) accession number: P03772
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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