P03772 (PP_LAMBD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Serine/threonine-protein phosphatase EC=3.1.3.16
Organism	Enterobacteria phage lambda (Bacteriophage lambda) [Reference proteome]
Taxonomic identifier	10710 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambdalikevirus
Virus host	Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 221

221

Serine/threonine-protein phosphatase

PRO_0000058912

Sites

Active site

76

1

Proton donor By similarity

Metal binding

20

1

Iron By similarity

Metal binding

22

1

Iron By similarity

Metal binding

49

1

Iron By similarity

Metal binding

49

1

Manganese By similarity

Metal binding

75

1

Manganese By similarity

Metal binding

186

1

Manganese By similarity

Secondary structure

1

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221

Helix Strand Turn

Details...

Beta strand

3 – 8

6

Helix

9 – 11

3

Beta strand

15 – 18

4

Helix

25 – 35

11

Turn

39 – 41

3

Beta strand

43 – 46

4

Beta strand

51 – 55

5

Helix

57 – 61

5

Helix

62 – 65

4

Beta strand

69 – 71

3

Helix

75 – 84

10

Helix

91 – 94

4

Turn

95 – 97

3

Helix

98 – 103

6

Helix

106 – 119

14

Beta strand

124 – 130

7

Beta strand

133 – 137

5

Beta strand

144 – 146

3

Helix

155 – 160

6

Helix

163 – 169

7

Beta strand

179 – 184

6

Beta strand

193 – 195

3

Beta strand

198 – 200

3

Helix

205 – 208

4

Beta strand

213 – 217

5

Sequences

	Sequence		Length	Mass (Da)
	P03772 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 5CE0E1A0F3BC5CB5	FASTA	221	25,219
	10 20 30 40 50 60 MRYYEKIDGS KYRNIWVVGD LHGCYTNLMN KLDTIGFDNK KDLLISVGDL VDRGAENVEC 70 80 90 100 110 120 LELITFPWFR AVRGNHEQMM IDGLSERGNV NHWLLNGGGW FFNLDYDKEI LAKALAHKAD 130 140 150 160 170 180 ELPLIIELVS KDKKYVICHA DYPFDEYEFG KPVDHQQVIW NRERISNSQN GIVKEIKGAD 190 200 210 220 TFIFGHTPAV KPLKFANQMY IDTGAVFCGN LTLIQVQGEG A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of bacteriophage lambda DNA." Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B. J. Mol. Biol. 162:729-773(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Discovery of a protein phosphatase activity encoded in the genome of bacteriophage lambda. Probable identity with open reading frame 221." Cohen P.T.W., Cohen P. Biochem. J. 260:931-934(1989) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02459 Genomic DNA. Translation: AAA96594.1.

PIR

Q1BP1L. G43011.

RefSeq

NP_040641.1. NC_001416.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G5B	X-ray	2.15	A/B/C	1-221	[»]

ProteinModelPortal

P03772.

SMR

P03772. Positions 1-218.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-44028N.

IntAct

P03772. 2 interactions.

MINT

MINT-4053809.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2703476.

Phylogenomic databases

ProtClustDB

CLSP2343890.

Family and domain databases

InterPro

IPR004843. Metallo_PEstase_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]

Pfam

PF00149. Metallophos. 1 hit.
[Graphical view]

SMART

SM00156. PP2Ac. 1 hit.
[Graphical view]

PROSITE

PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P03772.

ChEMBL

CHEMBL3695.

EvolutionaryTrace

P03772.

Entry information

Entry name

PP_LAMBD

Accession

Primary (citable) accession number: P03772

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program