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P03772

- PP_LAMBD

UniProt

P03772 - PP_LAMBD

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Protein

Serine/threonine-protein phosphatase

Gene
N/A
Organism
Enterobacteria phage lambda (Bacteriophage lambda)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+1 PublicationNote: Binds 2 manganese ions per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi20 – 201Manganese 1
Metal bindingi22 – 221Manganese 1
Metal bindingi49 – 491Manganese 1
Metal bindingi49 – 491Manganese 2
Metal bindingi75 – 751Manganese 2
Active sitei76 – 761Proton donorBy similarity
Metal bindingi186 – 1861Manganese 2

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase (EC:3.1.3.16)
OrganismiEnterobacteria phage lambda (Bacteriophage lambda)
Taxonomic identifieri10710 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000001711: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221Serine/threonine-protein phosphatasePRO_0000058912Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Plk4Q647022EBI-4478820,EBI-2552433From a different organism.

Protein-protein interaction databases

DIPiDIP-44028N.
IntActiP03772. 3 interactions.
MINTiMINT-4053809.

Structurei

Secondary structure

1
221
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi9 – 113Combined sources
Beta strandi15 – 184Combined sources
Helixi25 – 3511Combined sources
Turni39 – 413Combined sources
Beta strandi43 – 464Combined sources
Beta strandi51 – 555Combined sources
Helixi57 – 615Combined sources
Helixi62 – 654Combined sources
Beta strandi69 – 713Combined sources
Helixi75 – 8410Combined sources
Helixi91 – 944Combined sources
Turni95 – 973Combined sources
Helixi98 – 1036Combined sources
Helixi106 – 11914Combined sources
Beta strandi124 – 1307Combined sources
Beta strandi133 – 1375Combined sources
Beta strandi144 – 1463Combined sources
Helixi155 – 1606Combined sources
Helixi163 – 1697Combined sources
Beta strandi179 – 1846Combined sources
Beta strandi193 – 1953Combined sources
Beta strandi198 – 2003Combined sources
Helixi205 – 2084Combined sources
Beta strandi213 – 2175Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G5BX-ray2.15A/B/C1-221[»]
ProteinModelPortaliP03772.
SMRiP03772. Positions 1-218.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03772.

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family.Curated

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03772-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRYYEKIDGS KYRNIWVVGD LHGCYTNLMN KLDTIGFDNK KDLLISVGDL
60 70 80 90 100
VDRGAENVEC LELITFPWFR AVRGNHEQMM IDGLSERGNV NHWLLNGGGW
110 120 130 140 150
FFNLDYDKEI LAKALAHKAD ELPLIIELVS KDKKYVICHA DYPFDEYEFG
160 170 180 190 200
KPVDHQQVIW NRERISNSQN GIVKEIKGAD TFIFGHTPAV KPLKFANQMY
210 220
IDTGAVFCGN LTLIQVQGEG A
Length:221
Mass (Da):25,219
Last modified:July 21, 1986 - v1
Checksum:i5CE0E1A0F3BC5CB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA. Translation: AAA96594.1.
PIRiG43011. Q1BP1L.
RefSeqiNP_040641.1. NC_001416.1.

Genome annotation databases

GeneIDi2703476.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA. Translation: AAA96594.1 .
PIRi G43011. Q1BP1L.
RefSeqi NP_040641.1. NC_001416.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G5B X-ray 2.15 A/B/C 1-221 [» ]
ProteinModelPortali P03772.
SMRi P03772. Positions 1-218.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-44028N.
IntActi P03772. 3 interactions.
MINTi MINT-4053809.

Chemistry

BindingDBi P03772.
ChEMBLi CHEMBL3695.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2703476.

Miscellaneous databases

EvolutionaryTracei P03772.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Discovery of a protein phosphatase activity encoded in the genome of bacteriophage lambda. Probable identity with open reading frame 221."
    Cohen P.T.W., Cohen P.
    Biochem. J. 260:931-934(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Structure of the bacteriophage lambda Ser/Thr protein phosphatase with sulfate ion bound in two coordination modes."
    Voegtli W.C., White D.J., Reiter N.J., Rusnak F., Rosenzweig A.C.
    Biochemistry 39:15365-15374(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) IN COMPLEX WITH MANGANESE IONS, COFACTOR.

Entry informationi

Entry nameiPP_LAMBD
AccessioniPrimary (citable) accession number: P03772
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3