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Protein

Peptidoglycan hydrolase gp16

Gene

16

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cylindrical core that assembles on the inner surface of the capsid during procapsid formation. Plays a role in ejection of the bacteriophage DNA into the host cell at the initiation of infection. Functions as an exolysin that catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycans.3 Publications

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei37 – 371PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • entry into host via enzymatic degradation of host anatomical structure Source: UniProtKB
  • peptidoglycan metabolic process Source: InterPro
  • viral genome ejection through host cell envelope Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase, Viral short tail ejection system

Keywords - Biological processi

Degradation of host cell envelope components during virus entry, Degradation of host peptidoglycans during virus entry, Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Virus entry into host cell

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.
TCDBi3.A.17.1.1. the phage t7 injectisome (t7 injectisome) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan hydrolase gp16 (EC:4.2.2.n1)
Alternative name(s):
Gene product 16
Short name:
Gp16
Internal virion protein gp16
Gene namesi
Ordered Locus Names:16
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13181318Peptidoglycan hydrolase gp16PRO_0000196560Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Interacts with gp15 octamer. The T7 inner core is composed of stacked rings of three distinct proteins gp14, gp15 and gp16.1 Publication

Protein-protein interaction databases

IntActiP03726. 1 interaction.
MINTiMINT-1513705.

Structurei

3D structure databases

ProteinModelPortaliP03726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 11188Slt-type domainAdd
BLAST

Sequence similaritiesi

Belongs to the transglycosylase Slt family.Curated

Family and domain databases

InterProiIPR023346. Lysozyme-like_dom.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
[Graphical view]
PfamiPF01464. SLT. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00922. TRANSGLYCOSYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKYDKNVPS DYDGLFQKAA DANGVSYDLL RKVAWTESRF VPTAKSKTGP
60 70 80 90 100
LGMMQFTKAT AKALGLRVTD GPDDDRLNPE LAINAAAKQL AGLVGKFDGD
110 120 130 140 150
ELKAALAYNQ GEGRLGNPQL EAYSKGDFAS ISEEGRNYMR NLLDVAKSPM
160 170 180 190 200
AGQLETFGGI TPKGKGIPAE VGLAGIGHKQ KVTQELPEST SFDVKGIEQE
210 220 230 240 250
ATAKPFAKDF WETHGETLDE YNSRSTFFGF KNAAEAELSN SVAGMAFRAG
260 270 280 290 300
RLDNGFDVFK DTITPTRWNS HIWTPEELEK IRTEVKNPAY INVVTGGSPE
310 320 330 340 350
NLDDLIKLAN ENFENDSRAA EAGLGAKLSA GIIGAGVDPL SYVPMVGVTG
360 370 380 390 400
KGFKLINKAL VVGAESAALN VASEGLRTSV AGGDADYAGA ALGGFVFGAG
410 420 430 440 450
MSAISDAVAA GLKRSKPEAE FDNEFIGPMM RLEARETARN ANSADLSRMN
460 470 480 490 500
TENMKFEGEH NGVPYEDLPT ERGAVVLHDG SVLSASNPIN PKTLKEFSEV
510 520 530 540 550
DPEKAARGIK LAGFTEIGLK TLGSDDADIR RVAIDLVRSP TGMQSGASGK
560 570 580 590 600
FGATASDIHE RLHGTDQRTY NDLYKAMSDA MKDPEFSTGG AKMSREETRY
610 620 630 640 650
TIYRRAALAI ERPELQKALT PSERIVMDII KRHFDTKREL MENPAIFGNT
660 670 680 690 700
KAVSIFPESR HKGTYVPHVY DRHAKALMIQ RYGAEGLQEG IARSWMNSYV
710 720 730 740 750
SRPEVKARVD EMLKELHGVK EVTPEMVEKY AMDKAYGISH SDQFTNSSII
760 770 780 790 800
EENIEGLVGI ENNSFLEARN LFDSDLSITM PDGQQFSVND LRDFDMFRIM
810 820 830 840 850
PAYDRRVNGD IAIMGSTGKT TKELKDEILA LKAKAEGDGK KTGEVHALMD
860 870 880 890 900
TVKILTGRAR RNQDTVWETS LRAINDLGFF AKNAYMGAQN ITEIAGMIVT
910 920 930 940 950
GNVRALGHGI PILRDTLYKS KPVSAKELKE LHASLFGKEV DQLIRPKRAD
960 970 980 990 1000
IVQRLREATD TGPAVANIVG TLKYSTQELA ARSPWTKLLN GTTNYLLDAA
1010 1020 1030 1040 1050
RQGMLGDVIS ATLTGKTTRW EKEGFLRGAS VTPEQMAGIK SLIKEHMVRG
1060 1070 1080 1090 1100
EDGKFTVKDK QAFSMDPRAM DLWRLADKVA DEAMLRPHKV SLQDSHAFGA
1110 1120 1130 1140 1150
LGKMVMQFKS FTIKSLNSKF LRTFYDGYKN NRAIDAALSI ITSMGLAGGF
1160 1170 1180 1190 1200
YAMAAHVKAY ALPKEKRKEY LERALDPTMI AHAALSRSSQ LGAPLAMVDL
1210 1220 1230 1240 1250
VGGVLGFESS KMARSTILPK DTVKERDPNK PYTSREVMGA MGSNLLEQMP
1260 1270 1280 1290 1300
SAGFVANVGA TLMNAAGVVN SPNKATEQDF MTGLMNSTKE LVPNDPLTQQ
1310
LVLKIYEANG VNLRERRK
Length:1,318
Mass (Da):143,838
Last modified:July 21, 1986 - v1
Checksum:i51A0AAA920CBF210
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24434.1.
PIRiA04352. HIBPD7.
RefSeqiNP_042004.1. NC_001604.1.

Genome annotation databases

GeneIDi1261031.
KEGGivg:1261031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24434.1.
PIRiA04352. HIBPD7.
RefSeqiNP_042004.1. NC_001604.1.

3D structure databases

ProteinModelPortaliP03726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03726. 1 interaction.
MINTiMINT-1513705.

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.
TCDBi3.A.17.1.1. the phage t7 injectisome (t7 injectisome) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261031.
KEGGivg:1261031.

Family and domain databases

InterProiIPR023346. Lysozyme-like_dom.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
[Graphical view]
PfamiPF01464. SLT. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00922. TRANSGLYCOSYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEXLYS_BPT7
AccessioniPrimary (citable) accession number: P03726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 8, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.