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Protein

Capsid assembly protease C

Gene

C

more
Organism
Enterobacteria phage lambda (Bacteriophage lambda)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Assembly protease promotes icosahedral procapsid assembly. Autocatalytic cleavage may release the capsid scaffolding protein. The protease domain catalyzes the cleavage of the capsid scaffolding protein after complete procapsid formation. Assembly protease and cleavages products are evicted from the capsid before or during DNA packaging.
Scaffolding protein Nu3 promotes icosahedral procapsid assembly. Acts by binding the major capsid protein gpE and multimerizing in interaction with portal dodecamer, thereby placing gpE in a context facilitating icoahedral procapsid formation. Cleaved by capsid assembly protease C after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661NucleophileBy similarity

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • serine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Viral capsid assembly, Virus exit from host cell

Protein family/group databases

MEROPSiS49.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid assembly protease C (EC:3.4.21.-)
Alternative name(s):
Gene product C
Short name:
GPC
Minor capsid protein C
Gene namesi
Name:C
AND
Name:NU3
Ordered Locus Names:lambdap05
OrganismiEnterobacteria phage lambda (Bacteriophage lambda)
Taxonomic identifieri10710 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000001711 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi166 – 1661S → A: Complete loss of protease activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Capsid assembly protease CPRO_0000027358Add
BLAST

Interactioni

Subunit structurei

Capsid assembly protease interacts with capsid scaffolding protein Nu3. Capsid scaffolding protein Nu3 multimerizes and interacts with major capsid protein gpE.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-4478358,EBI-4478358

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

IntActiP03711. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliP03711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S49 family.Curated

Family and domain databases

Gene3Di3.90.226.10. 2 hits.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR002142. Peptidase_S49.
[Graphical view]
PfamiPF01343. Peptidase_S49. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Capsid assembly protease C (identifier: P03711-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAELRNLPH IASMAFNEPL MLEPAYARVF FCALAGQLGI SSLTDAVSGD
60 70 80 90 100
SLTAQEALAT LALSGDDDGP RQARSYQVMN GIAVLPVSGT LVSRTRALQP
110 120 130 140 150
YSGMTGYNGI IARLQQAASD PMVDGILLDM DTPGGMVAGA FDCADIIARV
160 170 180 190 200
RDIKPVWALA NDMNCSAGQL LASAASRRLV TQTARTGSIG VMMAHSNYGA
210 220 230 240 250
ALEKQGVEIT LIYSGSHKVD GNPYSHLPDD VRETLQSRMD ATRQMFAQKV
260 270 280 290 300
SAYTGLSVQV VLDTEAAVYS GQEAIDAGLA DELVNSTDAI TVMRDALDAR
310 320 330 340 350
KSRLSGGRMT KETQSTTVSA TASQADVTDV VPATEGENAS AAQPDVNAQI
360 370 380 390 400
TAAVAAENSR IMGILNCEEA HGREEQARVL AETPGMTVKT ARRILAAAPQ
410 420 430
SAQARSDTAL DRLMQGAPAP LAAGNPASDA VNDLLNTPV
Length:439
Mass (Da):45,940
Last modified:July 21, 1986 - v1
Checksum:i355ED56D60F6CDDF
GO
Isoform Capsid scaffolding protein Nu3 (identifier: P03711-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-308: Missing.

Note: Produced by alternative initiation at Met-309 of isoform.
Show »
Length:131
Mass (Da):13,394
Checksum:iE4F1ECF65753ACEA
GO

Sequence cautioni

The sequence AAA96538 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 308308Missing in isoform Capsid scaffolding protein Nu3. CuratedVSP_054968Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA. Translation: AAA96537.1.
J02459 Genomic DNA. Translation: AAA96538.1. Different initiation.
PIRiA04333. VHBPCL.
RefSeqiNP_040584.1. NC_001416.1. [P03711-1]
NP_040585.1. NC_001416.1.

Genome annotation databases

GeneIDi2703527.
2703528.
KEGGivg:2703527.
vg:2703528.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA. Translation: AAA96537.1.
J02459 Genomic DNA. Translation: AAA96538.1. Different initiation.
PIRiA04333. VHBPCL.
RefSeqiNP_040584.1. NC_001416.1. [P03711-1]
NP_040585.1. NC_001416.1.

3D structure databases

ProteinModelPortaliP03711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03711. 4 interactions.

Protein family/group databases

MEROPSiS49.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703527.
2703528.
KEGGivg:2703527.
vg:2703528.

Family and domain databases

Gene3Di3.90.226.10. 2 hits.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR002142. Peptidase_S49.
[Graphical view]
PfamiPF01343. Peptidase_S49. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSCAF_LAMBD
AccessioniPrimary (citable) accession number: P03711
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 24, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

It is uncertain wether Val-260 or Met-309 is the initiator for Capsid scaffolding protein Nu3.

Caution

Some of the E protein may be covalently linked with an equimolar amount of protein C and cleaved to yield minor capsid proteins X1 and X2. But recent data fail to detect cleavages products.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.