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Protein

Terminase small subunit

Gene

Nu1

Organism
Enterobacteria phage lambda (Bacteriophage lambda)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the terminase that processes and encapsidates viral genomes during virion assembly. The terminase is composed of two small and one large subunits. To initiate packaging, it binds a specific sequence called cos, at the junction of adjacent viral genomes in the concatemeric DNA substrate. Next, in a reaction stimulated by the presence of proheads and ATP but not requiring ATP hydrolysis, the terminase creates two nicks 12bp appart at the cos site, one on each stand. Terminase then separates the cohesive ends in a reaction requiring ATP hydrolysis. The heterotrimer remains bound to the left end of the genome to be packaged, forming a stable DNA-protein complex known as complex I. In a reaction facilitated by a viral assembly catalyst, gpFI, complex I binds a prohead, a preformed head shell precursor, to form complex II. In another packaging reaction requiring ATP hydrolysis, the DNA is translocated into the prohead until the next cos site on the concatemer reaches the packaging complex. At this time the downstream cos site is cut and the heterotrimer undocks from the DNA-filled head to remain bound to the left end of concatemer's next genome. The new heterotrimer-DNA complex I binds another prohead to continue the processive, polarized packaging of viral genomes. The terminase is dependent upon host integration host factor (ihfA/ihfB) for these activities.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 366ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Viral genome packaging, Virus exit from host cell

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Terminase small subunit
Alternative name(s):
DNA-packaging protein Nu1
Gene product Nu1
Short name:
gpNu1
Gene namesi
Name:Nu1
Ordered Locus Names:lambdap01
OrganismiEnterobacteria phage lambda (Bacteriophage lambda)
Taxonomic identifieri10710 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000001711 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Terminase small subunitPRO_0000077675Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of two small and one large terminase subunits. The active complex may be composed of five heterotrimers.1 Publication

Protein-protein interaction databases

IntActiP03707. 6 interactions.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 43Combined sources
Helixi5 – 117Combined sources
Helixi16 – 227Combined sources
Turni23 – 264Combined sources
Beta strandi35 – 373Combined sources
Beta strandi40 – 423Combined sources
Helixi43 – 508Combined sources
Turni51 – 533Combined sources
Helixi54 – 6310Combined sources
Beta strandi64 – 663Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J9INMR-A/B1-68[»]
ProteinModelPortaliP03707.
SMRiP03707. Positions 1-68.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03707.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili50 – 10960Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the terminase small subunit family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR010906. Phage_lambda_Nu1_terminase-ssu.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF07471. Phage_Nu1. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.

Sequencei

Sequence statusi: Complete.

P03707-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVNKKQLAD IFGASIRTIQ NWQEQGMPVL RGGGKGNEVL YDSAAVIKWY
60 70 80 90 100
AERDAEIENE KLRREVEELR QASEADLQPG TIEYERHRLT RAQADAQELK
110 120 130 140 150
NARDSAEVVE TAFCTFVLSR IAGEIASILD GLPLSVQRRF PELENRHVDF
160 170 180
LKRDIIKAMN KAAALDELIP GLLSEYIEQS G
Length:181
Mass (Da):20,441
Last modified:July 21, 1986 - v1
Checksum:iA25A5EAEF7BF18A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA. Translation: AAA96533.1.
PIRiC04333. JVBPNL.
RefSeqiNP_040580.1. NC_001416.1.

Genome annotation databases

GeneIDi2703523.
KEGGivg:2703523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA. Translation: AAA96533.1.
PIRiC04333. JVBPNL.
RefSeqiNP_040580.1. NC_001416.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J9INMR-A/B1-68[»]
ProteinModelPortaliP03707.
SMRiP03707. Positions 1-68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03707. 6 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703523.
KEGGivg:2703523.

Miscellaneous databases

EvolutionaryTraceiP03707.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR010906. Phage_lambda_Nu1_terminase-ssu.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF07471. Phage_Nu1. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The terminase of bacteriophage lambda. Functional domains for cosB binding and multimer assembly."
    Frackman S., Siegele D.A., Feiss M.
    J. Mol. Biol. 183:225-238(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Prediction of an ATP reactive center in the small subunit, gpNu1, of the phage lambda terminase enzyme."
    Becker A., Gold M.
    J. Mol. Biol. 199:219-222(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: ATP-BINDING SITE.
  4. "Self-association properties of the bacteriophage lambda terminase holoenzyme: implications for the DNA packaging motor."
    Maluf N.K., Yang Q., Catalano C.E.
    J. Mol. Biol. 347:523-542(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  5. "Insights into specific DNA recognition during the assembly of a viral genome packaging machine."
    de Beer T., Fang J., Ortega M., Yang Q., Maes L., Duffy C., Berton N., Sippy J., Overduin M., Feiss M., Catalano C.E.
    Mol. Cell 9:981-991(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-68.

Entry informationi

Entry nameiTERS_LAMBD
AccessioniPrimary (citable) accession number: P03707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: December 9, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.