P03706 (LYS_LAMBD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme
EC=3.2.1.17

Alternative name(s):
Endolysin
Lysis protein
Muramidase

Gene names

Name:

R

Organism

Enterobacteria phage lambda (Bacteriophage lambda) [Reference proteome]

Taxonomic identifier

10710 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambdalikevirus

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity. Acts as a transglycosylase. Cleaves glycosidic bonds between the C1 of N-acetyl muramic acids (NAM) and C4 of N-acetyl glucosamines (NAG) of the peptidoglycan of the bacterial walls.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Sequence similarities	Belongs to the glycosyl hydrolase 24 family.

Ontologies

Keywords
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 158

158

Lysozyme

PRO_0000218108

Sites

Active site

19

1

Experimental info

Sequence conflict

74

1

Missing AA sequence Ref.2

Secondary structure

1

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158

Helix Strand Turn

Details...

Helix

6 – 18

13

Beta strand

21 – 26

6

Turn

31 – 34

4

Beta strand

53 – 56

4

Beta strand

59 – 61

3

Turn

65 – 68

4

Helix

71 – 81

11

Helix

88 – 101

14

Helix

105 – 110

6

Helix

113 – 120

8

Turn

121 – 123

3

Beta strand

132 – 134

3

Helix

135 – 148

14

Sequences

	Sequence		Length	Mass (Da)
	P03706 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 94F8E9A512EAA03E	FASTA	158	17,825
	10 20 30 40 50 60 MVEINNQRKA FLDMLAWSEG TDNGRQKTRN HGYDVIVGGE LFTDYSDHPR KLVTLNPKLK 70 80 90 100 110 120 STGAGRYQLL SRWWDAYRKQ LGLKDFSPKS QDAVALQQIK ERGALPMIDR GDIRQAIDRC 130 140 150 SNIWASLPGA GYGQFEHKAD SLIAKFKEAG GTVREIDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of bacteriophage lambda DNA." Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B. J. Mol. Biol. 162:729-773(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Amino-acid sequence of lambda phage endolysin." Imada M., Tsugita A. Nature New Biol. 233:230-231(1971) Cited for: PROTEIN SEQUENCE.
[3]	"Crystal structure of the lysozyme from bacteriophage lambda and its relationship with V and C-type lysozymes." Evrard C., Fastrez J., Declercq J.-P. J. Mol. Biol. 276:151-164(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02459 Genomic DNA. Translation: AAA96598.1.

PIR

EYBPL. B04333.

RefSeq

NP_040645.1. NC_001416.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AM7	X-ray	2.30	A/B/C	1-158	[»]
1D9U	X-ray	2.60	A/B	1-154	[»]
3D3D	X-ray	2.60	A/B	1-154	[»]

ProteinModelPortal

P03706.

SMR

P03706. Positions 1-154.

ModBase

Search...

Protein-protein interaction databases

IntAct

P03706. 1 interaction.

Protein family/group databases

CAZy

GH104. Glycoside Hydrolase Family 104.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2703480.

Phylogenomic databases

ProtClustDB

CLSP2343790.

Family and domain databases

InterPro

IPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
[Graphical view]

Pfam

PF00959. Phage_lysozyme. 1 hit.
[Graphical view]

SUPFAM

SSF53955. SSF53955. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P03706.

Entry information

Entry name

LYS_LAMBD

Accession

Primary (citable) accession number: P03706

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program