Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P03706 (LYS_LAMBD) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysozyme

EC=3.2.1.17
Alternative name(s):
Endolysin
Lysis protein
Muramidase
Gene names
Name:R
OrganismEnterobacteria phage lambda (Bacteriophage lambda) [Reference proteome]
Taxonomic identifier10710 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length158 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity. Acts as a transglycosylase. Cleaves glycosidic bonds between the C1 of N-acetyl muramic acids (NAM) and C4 of N-acetyl glucosamines (NAG) of the peptidoglycan of the bacterial walls.

Catalytic activity

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Subunit structure

Monomer.

Sequence similarities

Belongs to the glycosyl hydrolase 24 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 158158Lysozyme
PRO_0000218108

Sites

Active site191

Experimental info

Sequence conflict741Missing AA sequence Ref.2

Secondary structure

......................... 158
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P03706 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 94F8E9A512EAA03E

FASTA15817,825
        10         20         30         40         50         60 
MVEINNQRKA FLDMLAWSEG TDNGRQKTRN HGYDVIVGGE LFTDYSDHPR KLVTLNPKLK 

        70         80         90        100        110        120 
STGAGRYQLL SRWWDAYRKQ LGLKDFSPKS QDAVALQQIK ERGALPMIDR GDIRQAIDRC 

       130        140        150 
SNIWASLPGA GYGQFEHKAD SLIAKFKEAG GTVREIDV 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of bacteriophage lambda DNA."
Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.
J. Mol. Biol. 162:729-773(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Amino-acid sequence of lambda phage endolysin."
Imada M., Tsugita A.
Nature New Biol. 233:230-231(1971)
Cited for: PROTEIN SEQUENCE.
[3]"Crystal structure of the lysozyme from bacteriophage lambda and its relationship with V and C-type lysozymes."
Evrard C., Fastrez J., Declercq J.-P.
J. Mol. Biol. 276:151-164(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02459 Genomic DNA. Translation: AAA96598.1.
PIREYBPL. B04333.
RefSeqNP_040645.1. NC_001416.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AM7X-ray2.30A/B/C1-158[»]
1D9UX-ray2.60A/B1-154[»]
3D3DX-ray2.60A/B1-154[»]
ProteinModelPortalP03706.
SMRP03706. Positions 1-154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP03706. 1 interaction.

Protein family/group databases

CAZyGH104. Glycoside Hydrolase Family 104.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703480.

Phylogenomic databases

ProtClustDBCLSP2343790.

Family and domain databases

InterProIPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamPF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP03706.

Entry information

Entry nameLYS_LAMBD
AccessionPrimary (citable) accession number: P03706
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 16, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries