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Protein

Bacterial RNA polymerase inhibitor

Gene

2

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an inhibitor of bacterial RNA polymerase by interacting with RpoC subunit and thus preventing promoter recognition by RpoE. Non-DNA-binding transcription factor that plays a role in the transcriptional switch that abolishes transcription of the viral early genes (class I) by the host RNA polymerase but allows transcription of late genes (class II and class III) by T7 RNA polymerase.1 Publication

GO - Biological processi

  • suppression by virus of host transcription Source: UniProtKB

Keywordsi

Biological processBacterial host gene expression shutoff by virus, Bacterial host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Bacterial RNA polymerase inhibitor
Alternative name(s):
Gene product 2
Short name:
Gp2
Gene namesi
Ordered Locus Names:2
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001064811 – 64Bacterial RNA polymerase inhibitorAdd BLAST64

Interactioni

Subunit structurei

Interacts with host RpoC; this interaction inhibits host RNA polymerase activity.1 Publication

Protein-protein interaction databases

IntActiP03704. 1 interactor.
MINTiMINT-1513464.

Structurei

Secondary structure

164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 21Combined sources9
Beta strandi26 – 31Combined sources6
Helixi36 – 45Combined sources10
Helixi48 – 50Combined sources3
Beta strandi53 – 60Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LMCNMR-A1-60[»]
2WNMNMR-A1-64[»]
4LK0X-ray3.91M/N1-64[»]
4LLGX-ray3.79M/N1-64[»]
SMRiP03704.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03704.

Family & Domainsi

Family and domain databases

InterProiView protein in InterPro
IPR016412. RNA_pol_inhibitor.
PfamiView protein in Pfam
PF16857. RNA_pol_inhib. 1 hit.
PIRSFiPIRSF004348. RNA_polymerase_inhibitor_gp2. 1 hit.

Sequencei

Sequence statusi: Complete.

P03704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVNTGSLS VDNKKFWATV ESSEHSFEVP IYAETLDEAL ELAEWQYVPA
60
GFEVTRVRPC VAPK
Length:64
Mass (Da):7,175
Last modified:July 21, 1986 - v1
Checksum:iD42C11A4C116862F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24399.1.
V01127 Genomic DNA. Translation: CAA24342.1.
PIRiG94615. JRBP27.
RefSeqiNP_041969.1. NC_001604.1.

Genome annotation databases

GeneIDi1261073.
KEGGivg:1261073.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiVRPI_BPT7
AccessioniPrimary (citable) accession number: P03704
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 18, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references