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Protein

Integrase

Gene

int

Organism
Enterobacteria phage lambda (Bacteriophage lambda)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.1 Publication1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei342O-(3'-phospho-DNA)-tyrosine intermediateCombined sources1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination, Viral genome excision, Viral genome integration, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Integrase (EC:2.7.7.-1 Publication, EC:3.1.-.-1 Publication)
Gene namesi
Name:int
OrganismiEnterobacteria phage lambda (Bacteriophage lambda)
Taxonomic identifieri10710 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000001711 Componenti: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47E → A: Complete loss of interaction with the integrase. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975271 – 356IntegraseAdd BLAST356

Proteomic databases

PRIDEiP03700.

Interactioni

Subunit structurei

Homotetramer. Interacts (via N-terminus) with the excisionase (via C-terminus) (PubMed:12832614). Part of the excision complex made of the integrase tetramer, IHF, Fis and Xis.2 Publications

Protein-protein interaction databases

DIPiDIP-41000N.
IntActiP03700. 9 interactors.
MINTiMINT-120644.

Structurei

Secondary structure

1356
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Beta strandi16 – 18Combined sources3
Beta strandi24 – 27Combined sources4
Turni29 – 31Combined sources3
Beta strandi34 – 39Combined sources6
Helixi41 – 57Combined sources17
Helixi76 – 90Combined sources15
Helixi94 – 110Combined sources17
Helixi116 – 118Combined sources3
Helixi121 – 133Combined sources13
Helixi137 – 156Combined sources20
Turni164 – 167Combined sources4
Helixi182 – 191Combined sources10
Helixi192 – 194Combined sources3
Helixi198 – 209Combined sources12
Helixi213 – 218Combined sources6
Helixi221 – 223Combined sources3
Beta strandi228 – 232Combined sources5
Turni234 – 236Combined sources3
Beta strandi239 – 243Combined sources5
Turni249 – 252Combined sources4
Helixi255 – 265Combined sources11
Beta strandi269 – 272Combined sources4
Helixi282 – 296Combined sources15
Beta strandi301 – 303Combined sources3
Helixi309 – 321Combined sources13
Helixi324 – 331Combined sources8
Beta strandi334 – 336Combined sources3
Helixi339 – 342Combined sources4
Beta strandi349 – 352Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AE9X-ray1.90A/B177-355[»]
1KJKNMR-A1-64[»]
1M97model-A76-171[»]
1P7DX-ray2.95A/B74-356[»]
1Z19X-ray2.80A/B74-356[»]
1Z1BX-ray3.80A/B1-356[»]
1Z1GX-ray4.40A/B/C/D1-356[»]
2OXOX-ray2.00A75-176[»]
2WCCNMR-31-64[»]
ProteinModelPortaliP03700.
SMRiP03700.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03700.

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Family and domain databases

Gene3Di1.10.150.130. 1 hit.
1.10.443.10. 1 hit.
3.30.160.60. 1 hit.
InterProiIPR016177. DNA-bd_dom.
IPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
IPR015094. Integrase_lambda-typ_DNA-bd_N.
IPR023109. Integrase_recombinase_N.
IPR004107. Integrase_SAM-like_N.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02899. Phage_int_SAM_1. 1 hit.
PF09003. Phage_integ_N. 1 hit.
PF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
SSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

P03700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRRRSHERR DLPPNLYIRN NGYYCYRDPR TGKEFGLGRD RRIAITEAIQ
60 70 80 90 100
ANIELFSGHK HKPLTARINS DNSVTLHSWL DRYEKILASR GIKQKTLINY
110 120 130 140 150
MSKIKAIRRG LPDAPLEDIT TKEIAAMLNG YIDEGKAASA KLIRSTLSDA
160 170 180 190 200
FREAIAEGHI TTNHVAATRA AKSEVRRSRL TADEYLKIYQ AAESSPCWLR
210 220 230 240 250
LAMELAVVTG QRVGDLCEMK WSDIVDGYLY VEQSKTGVKI AIPTALHIDA
260 270 280 290 300
LGISMKETLD KCKEILGGET IIASTRREPL SSGTVSRYFM RARKASGLSF
310 320 330 340 350
EGDPPTFHEL RSLSARLYEK QISDKFAQHL LGHKSDTMAS QYRDDRGREW

DKIEIK
Length:356
Mass (Da):40,304
Last modified:July 21, 1986 - v1
Checksum:i708D52B96E89209C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti118D → V (PubMed:6253947).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA. Translation: AAA96562.1.
PIRiA04322. RSBPIL.
RefSeqiNP_040609.1. NC_001416.1.

Genome annotation databases

GeneIDi2703464.
KEGGivg:2703470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA. Translation: AAA96562.1.
PIRiA04322. RSBPIL.
RefSeqiNP_040609.1. NC_001416.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AE9X-ray1.90A/B177-355[»]
1KJKNMR-A1-64[»]
1M97model-A76-171[»]
1P7DX-ray2.95A/B74-356[»]
1Z19X-ray2.80A/B74-356[»]
1Z1BX-ray3.80A/B1-356[»]
1Z1GX-ray4.40A/B/C/D1-356[»]
2OXOX-ray2.00A75-176[»]
2WCCNMR-31-64[»]
ProteinModelPortaliP03700.
SMRiP03700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-41000N.
IntActiP03700. 9 interactors.
MINTiMINT-120644.

Proteomic databases

PRIDEiP03700.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703464.
KEGGivg:2703470.

Miscellaneous databases

EvolutionaryTraceiP03700.

Family and domain databases

Gene3Di1.10.150.130. 1 hit.
1.10.443.10. 1 hit.
3.30.160.60. 1 hit.
InterProiIPR016177. DNA-bd_dom.
IPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
IPR015094. Integrase_lambda-typ_DNA-bd_N.
IPR023109. Integrase_recombinase_N.
IPR004107. Integrase_SAM-like_N.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02899. Phage_int_SAM_1. 1 hit.
PF09003. Phage_integ_N. 1 hit.
PF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
SSF56349. SSF56349. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVINT_LAMBD
AccessioniPrimary (citable) accession number: P03700
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.