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Protein

Integrase

Gene

int

Organism
Escherichia phage lambda (Bacteriophage lambda)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.1 Publication1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei342O-(3'-phospho-DNA)-tyrosine intermediateCombined sources1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transferase
Biological processDNA integration, DNA recombination, Viral genome excision, Viral genome integration, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Integrase (EC:2.7.7.-1 Publication, EC:3.1.-.-1 Publication)
Gene namesi
Name:int
OrganismiEscherichia phage lambda (Bacteriophage lambda)
Taxonomic identifieri10710 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdavirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000001711 Componenti: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47E → A: Complete loss of interaction with the integrase. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975271 – 356IntegraseAdd BLAST356

Proteomic databases

PRIDEiP03700

Interactioni

Subunit structurei

Homotetramer. Interacts (via N-terminus) with the excisionase (via C-terminus) (PubMed:12832614). Part of the excision complex made of the integrase tetramer, IHF, Fis and Xis.2 Publications

Protein-protein interaction databases

DIPiDIP-41000N
IntActiP03700 10 interactors.

Structurei

Secondary structure

1356
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Beta strandi16 – 18Combined sources3
Beta strandi24 – 27Combined sources4
Turni29 – 31Combined sources3
Beta strandi34 – 39Combined sources6
Helixi41 – 57Combined sources17
Helixi76 – 90Combined sources15
Helixi94 – 110Combined sources17
Helixi116 – 118Combined sources3
Helixi121 – 133Combined sources13
Helixi137 – 156Combined sources20
Turni164 – 167Combined sources4
Helixi182 – 191Combined sources10
Helixi192 – 194Combined sources3
Helixi198 – 209Combined sources12
Helixi213 – 218Combined sources6
Helixi221 – 223Combined sources3
Beta strandi228 – 232Combined sources5
Turni234 – 236Combined sources3
Beta strandi239 – 243Combined sources5
Turni249 – 252Combined sources4
Helixi255 – 265Combined sources11
Beta strandi269 – 272Combined sources4
Helixi282 – 296Combined sources15
Beta strandi301 – 303Combined sources3
Helixi309 – 321Combined sources13
Helixi324 – 331Combined sources8
Beta strandi334 – 336Combined sources3
Helixi339 – 342Combined sources4
Beta strandi349 – 352Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AE9X-ray1.90A/B177-355[»]
1KJKNMR-A1-64[»]
1M97model-A76-171[»]
1P7DX-ray2.95A/B74-356[»]
1Z19X-ray2.80A/B74-356[»]
1Z1BX-ray3.80A/B1-356[»]
1Z1GX-ray4.40A/B/C/D1-356[»]
2OXOX-ray2.00A75-176[»]
2WCCNMR-31-64[»]
5J0Nelectron microscopy11.00E/F/G/H1-356[»]
ProteinModelPortaliP03700
SMRiP03700
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03700

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Phylogenomic databases

OrthoDBiVOG090000CQ

Family and domain databases

Gene3Di1.10.150.1301 hit
1.10.443.101 hit
InterProiView protein in InterPro
IPR016177 DNA-bd_dom_sf
IPR011010 DNA_brk_join_enz
IPR013762 Integrase-like_cat_sf
IPR002104 Integrase_catalytic
IPR015094 Integrase_lambda-typ_DNA-bd_N
IPR010998 Integrase_recombinase_N
IPR004107 Integrase_SAM-like_N
PfamiView protein in Pfam
PF09003 Arm-DNA-bind_1, 1 hit
PF02899 Phage_int_SAM_1, 1 hit
PF00589 Phage_integrase, 1 hit
SUPFAMiSSF54171 SSF54171, 1 hit
SSF56349 SSF56349, 1 hit

Sequencei

Sequence statusi: Complete.

P03700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRRRSHERR DLPPNLYIRN NGYYCYRDPR TGKEFGLGRD RRIAITEAIQ
60 70 80 90 100
ANIELFSGHK HKPLTARINS DNSVTLHSWL DRYEKILASR GIKQKTLINY
110 120 130 140 150
MSKIKAIRRG LPDAPLEDIT TKEIAAMLNG YIDEGKAASA KLIRSTLSDA
160 170 180 190 200
FREAIAEGHI TTNHVAATRA AKSEVRRSRL TADEYLKIYQ AAESSPCWLR
210 220 230 240 250
LAMELAVVTG QRVGDLCEMK WSDIVDGYLY VEQSKTGVKI AIPTALHIDA
260 270 280 290 300
LGISMKETLD KCKEILGGET IIASTRREPL SSGTVSRYFM RARKASGLSF
310 320 330 340 350
EGDPPTFHEL RSLSARLYEK QISDKFAQHL LGHKSDTMAS QYRDDRGREW

DKIEIK
Length:356
Mass (Da):40,304
Last modified:July 21, 1986 - v1
Checksum:i708D52B96E89209C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti118D → V (PubMed:6253947).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA Translation: AAA96562.1
PIRiA04322 RSBPIL
RefSeqiNP_040609.1, NC_001416.1

Genome annotation databases

GeneIDi2703464
KEGGivg:2703470

Similar proteinsi

Entry informationi

Entry nameiVINT_LAMBD
AccessioniPrimary (citable) accession number: P03700
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome