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P03697

- EXO_LAMBD

UniProt

P03697 - EXO_LAMBD

Protein

Exonuclease

Gene

exo

Organism
Enterobacteria phage lambda (Bacteriophage lambda)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (14 May 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Facilitates phage DNA recombination through the double-strand break repair (DSBR) and single-strand annealing pathways. Also important for the late, rolling-circle mode of lambda DNA replication.

    Catalytic activityi

    Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates.

    Cofactori

    Magnesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi119 – 1191Magnesium
    Metal bindingi129 – 1291Magnesium

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. exonuclease activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Exonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.1.11.3. 717.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exonuclease (EC:3.1.11.3)
    Gene namesi
    Name:exo
    Synonyms:red-alpha, redX
    OrganismiEnterobacteria phage lambda (Bacteriophage lambda)
    Taxonomic identifieri10710 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000001711: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 226226ExonucleasePRO_0000077574Add
    BLAST

    Interactioni

    Subunit structurei

    Trimer of three subunits that form a toroid, with a tapered channel passing through the middle.

    Protein-protein interaction databases

    DIPiDIP-59695N.

    Structurei

    Secondary structure

    1
    226
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 108
    Helixi14 – 163
    Beta strandi19 – 213
    Helixi22 – 276
    Turni28 – 303
    Helixi34 – 374
    Helixi38 – 414
    Beta strandi45 – 484
    Helixi52 – 6716
    Helixi75 – 9622
    Beta strandi106 – 1094
    Beta strandi114 – 1163
    Beta strandi119 – 1224
    Beta strandi127 – 1315
    Helixi136 – 14510
    Helixi146 – 1494
    Helixi151 – 16515
    Beta strandi168 – 1758
    Beta strandi179 – 1813
    Beta strandi184 – 1907
    Helixi193 – 21624
    Helixi223 – 2253

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AVQX-ray2.40A/B/C1-226[»]
    3SLPX-ray2.30A/B/C1-226[»]
    3SM4X-ray1.88A/B/C1-226[»]
    ProteinModelPortaliP03697.
    SMRiP03697. Positions 1-226.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03697.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.90.320.10. 1 hit.
    InterProiIPR011604. Exonuc_phg/RecB_C.
    IPR011335. Restrct_endonuc-II-like.
    IPR019080. YqaJ_viral_recombinase.
    [Graphical view]
    PfamiPF09588. YqaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P03697-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPDIILQRT GIDVRAVEQG DDAWHKLRLG VITASEVHNV IAKPRSGKKW    50
    PDMKMSYFHT LLAEVCTGVA PEVNAKALAW GKQYENDART LFEFTSGVNV 100
    TESPIIYRDE SMRTACSPDG LCSDGNGLEL KCPFTSRDFM KFRLGGFEAI 150
    KSAYMAQVQY SMWVTRKNAW YFANYDPRMK REGLHYVVIE RDEKYMASFD 200
    EIVPEFIEKM DEALAEIGFV FGEQWR 226
    Length:226
    Mass (Da):25,909
    Last modified:July 21, 1986 - v1
    Checksum:i7D93AABA17F9AE26
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02459 Genomic DNA. Translation: AAA96569.1.
    PIRiH94614. NDBPXL.
    RefSeqiNP_040616.1. NC_001416.1.

    Genome annotation databases

    GeneIDi2703469.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02459 Genomic DNA. Translation: AAA96569.1 .
    PIRi H94614. NDBPXL.
    RefSeqi NP_040616.1. NC_001416.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AVQ X-ray 2.40 A/B/C 1-226 [» ]
    3SLP X-ray 2.30 A/B/C 1-226 [» ]
    3SM4 X-ray 1.88 A/B/C 1-226 [» ]
    ProteinModelPortali P03697.
    SMRi P03697. Positions 1-226.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59695N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2703469.

    Enzyme and pathway databases

    BRENDAi 3.1.11.3. 717.

    Miscellaneous databases

    EvolutionaryTracei P03697.

    Family and domain databases

    Gene3Di 3.90.320.10. 1 hit.
    InterProi IPR011604. Exonuc_phg/RecB_C.
    IPR011335. Restrct_endonuc-II-like.
    IPR019080. YqaJ_viral_recombinase.
    [Graphical view ]
    Pfami PF09588. YqaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Toroidal structure of lambda-exonuclease."
      Kovall R., Matthews B.W.
      Science 277:1824-1827(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

    Entry informationi

    Entry nameiEXO_LAMBD
    AccessioniPrimary (citable) accession number: P03697
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: May 14, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3