P03697 (EXO_LAMBD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exonuclease
EC=3.1.11.3

Gene names

Name:	exo
Synonyms:	red-alpha, redX

Organism

Enterobacteria phage lambda (Bacteriophage lambda) [Reference proteome]

Taxonomic identifier

10710 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambdalikevirus

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Facilitates phage DNA recombination through the double-strand break repair (DSBR) and single-strand annealing pathways. Also important for the late, rolling-circle mode of lambda DNA replication.
Catalytic activity	Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates.
Cofactor	Magnesium.
Subunit structure	Trimer of three subunits that form a toroid, with a tapered channel passing through the middle.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 226

226

Exonuclease

PRO_0000077574

Sites

Metal binding

119

1

Magnesium

Metal binding

129

1

Magnesium

Secondary structure

1

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226

Helix Strand Turn

Details...

Helix

3 – 10

8

Helix

14 – 16

3

Beta strand

19 – 21

3

Helix

22 – 27

6

Turn

28 – 30

3

Helix

34 – 37

4

Helix

38 – 41

4

Beta strand

45 – 48

4

Helix

52 – 67

16

Helix

75 – 96

22

Beta strand

106 – 109

4

Beta strand

114 – 116

3

Beta strand

119 – 122

4

Beta strand

127 – 131

5

Helix

136 – 145

10

Helix

146 – 149

4

Helix

151 – 165

15

Beta strand

168 – 175

8

Beta strand

179 – 181

3

Beta strand

184 – 190

7

Helix

193 – 216

24

Helix

223 – 225

3

Sequences

	Sequence		Length	Mass (Da)
	P03697 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 7D93AABA17F9AE26	FASTA	226	25,909
	10 20 30 40 50 60 MTPDIILQRT GIDVRAVEQG DDAWHKLRLG VITASEVHNV IAKPRSGKKW PDMKMSYFHT 70 80 90 100 110 120 LLAEVCTGVA PEVNAKALAW GKQYENDART LFEFTSGVNV TESPIIYRDE SMRTACSPDG 130 140 150 160 170 180 LCSDGNGLEL KCPFTSRDFM KFRLGGFEAI KSAYMAQVQY SMWVTRKNAW YFANYDPRMK 190 200 210 220 REGLHYVVIE RDEKYMASFD EIVPEFIEKM DEALAEIGFV FGEQWR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of bacteriophage lambda DNA." Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B. J. Mol. Biol. 162:729-773(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Toroidal structure of lambda-exonuclease." Kovall R., Matthews B.W. Science 277:1824-1827(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02459 Genomic DNA. Translation: AAA96569.1.

PIR

NDBPXL. H94614.

RefSeq

NP_040616.1. NC_001416.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AVQ	X-ray	2.40	A/B/C	1-226	[»]
3SLP	X-ray	2.30	A/B/C	1-226	[»]
3SM4	X-ray	1.88	A/B/C	1-226	[»]

ProteinModelPortal

P03697.

SMR

P03697. Positions 1-226.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59695N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2703469.

Phylogenomic databases

ProtClustDB

CLSP2343950.

Enzyme and pathway databases

BRENDA

3.1.11.3. 717.

Family and domain databases

Gene3D

3.90.320.10. 1 hit.

InterPro

IPR011604. Exonuc_phg/RecB_C.
IPR011335. Restrct_endonuc-II-like.
IPR019080. YqaJ_viral_recombinase.
[Graphical view]

Pfam

PF09588. YqaJ. 1 hit.
[Graphical view]

SUPFAM

SSF52980. Restrict_endonuc_II-like_core. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P03697.

Entry information

Entry name

EXO_LAMBD

Accession

Primary (citable) accession number: P03697

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program