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Protein

Single-stranded DNA-binding protein gp2.5

Gene

2.5

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded DNA-binding protein that eliminates secondary structure in long ssDNA formed on the lagging strand of the replication fork. Stimulates DNA polymerase activity and increases the efficiency of RNA primer synthesis by interacting with the DNA polymerase and the helicase/primase protein gp4. Disrupts loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation.3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA recombination, DNA repair, DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Single-stranded DNA-binding protein gp2.5
Short name:
SSB protein
Gene namesi
Ordered Locus Names:2.5
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001064831 – 232Single-stranded DNA-binding protein gp2.5Add BLAST232

Interactioni

Subunit structurei

Homodimer. Interacts (via C-terminus) with the DNA polymerase gp5. Interacts with the helicase/primase gp4.3 Publications

Protein-protein interaction databases

IntActiP03696. 1 interactor.
MINTiMINT-1513401.

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 13Combined sources4
Beta strandi18 – 23Combined sources6
Beta strandi37 – 45Combined sources9
Helixi49 – 74Combined sources26
Beta strandi95 – 98Combined sources4
Beta strandi100 – 115Combined sources16
Turni117 – 119Combined sources3
Beta strandi122 – 124Combined sources3
Beta strandi148 – 158Combined sources11
Beta strandi166 – 179Combined sources14
Helixi191 – 193Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JE5X-ray1.90A/B1-206[»]
ProteinModelPortaliP03696.
SMRiP03696.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03696.

Family & Domainsi

Phylogenomic databases

OrthoDBiVOG090000VM.

Family and domain databases

InterProiView protein in InterPro
IPR016411. Helix_destablising_prot.
IPR012340. NA-bd_OB-fold.
PIRSFiPIRSF004311. Helix_destablz_SSB_T7. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.

Sequencei

Sequence statusi: Complete.

P03696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKIFTSAL GTAEPYAYIA KPDYGNEERG FGNPRGVYKV DLTIPNKDPR
60 70 80 90 100
CQRMVDEIVK CHEEAYAAAV EEYEANPPAV ARGKKPLKPY EGDMPFFDNG
110 120 130 140 150
DGTTTFKFKC YASFQDKKTK ETKHINLVVV DSKGKKMEDV PIIGGGSKLK
160 170 180 190 200
VKYSLVPYKW NTAVGASVKL QLESVMLVEL ATFGGGEDDW ADEVEENGYV
210 220 230
ASGSAKASKP RDEESWDEDD EESEEADEDG DF
Length:232
Mass (Da):25,694
Last modified:July 21, 1986 - v1
Checksum:i2D2EF0B5FB89F3F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24400.1.
V01127 Genomic DNA. Translation: CAA24343.1.
PIRiF94615. DDBPT7.
RefSeqiNP_041970.1. NC_001604.1.

Genome annotation databases

GeneIDi1261080.
KEGGivg:1261080.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDNBI_BPT7
AccessioniPrimary (citable) accession number: P03696
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 15, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references