Reviewed, UniProtKB/Swiss-Prot P03696 (VHED_BPT7)

Names and origin

Protein names

Recommended name:
    Helix-destabilizing protein
Alternative name(s):
    Single-stranded DNA-binding protein
      Short name=SSB protein

Gene names

Name:

2.5

Organism

Enterobacteria phage T7 (Bacteriophage T7)

Taxonomic identifier

10760 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae › T7-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Helix-destabilizing protein, which is expressed in the late stage of lytic development, binds preferentially to single-stranded DNA. It is implicated in DNA replication, recombination, and repair.
Subunit structure	Homodimer.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Ligand	DNA-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 232

232

Helix-destabilizing protein

PRO_0000106483

Secondary structure

1

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232

Helix Strand Turn

Details...

Beta strand

10 – 13

4

Beta strand

18 – 23

6

Beta strand

37 – 45

9

Helix

49 – 74

26

Beta strand

95 – 98

4

Beta strand

100 – 115

16

Turn

117 – 119

3

Beta strand

122 – 124

3

Beta strand

148 – 158

11

Beta strand

166 – 179

14

Helix

191 – 193

3

Sequences

	Sequence		Length	Mass (Da)
	P03696-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 2D2EF0B5FB89F3F4	FASTA	232	25,694
	10 20 30 40 50 60 MAKKIFTSAL GTAEPYAYIA KPDYGNEERG FGNPRGVYKV DLTIPNKDPR CQRMVDEIVK 70 80 90 100 110 120 CHEEAYAAAV EEYEANPPAV ARGKKPLKPY EGDMPFFDNG DGTTTFKFKC YASFQDKKTK 130 140 150 160 170 180 ETKHINLVVV DSKGKKMEDV PIIGGGSKLK VKYSLVPYKW NTAVGASVKL QLESVMLVEL 190 200 210 220 230 ATFGGGEDDW ADEVEENGYV ASGSAKASKP RDEESWDEDD EESEEADEDG DF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements." Dunn J.J., Studier F.W. J. Mol. Biol. 166:477-535(1983) [PubMed: 6864790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4." Dunn J.J., Studier F.W. J. Mol. Biol. 148:303-330(1981) [PubMed: 7310871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Purification and characterization of the bacteriophage T7 gene 2.5 protein. A single-stranded DNA-binding protein." Kim Y.T., Tabor S., Bortner C., Grifith J.D., Richardson C.C. J. Biol. Chem. 267:15022-15031(1992) [PubMed: 1634538] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

V01146 Genomic DNA. Translation: CAA24400.1.
V01127 Genomic DNA. Translation: CAA24343.1.

PIR

DDBPT7. F94615.

RefSeq

NP_041970.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JE5	X-ray	1.90	A/B	1-206	[»]

ModBase

Search...

Genome annotation databases

GeneID

1261080.

GenomeReviews

Gene locus 2.5 in contig V01146_GR.

Family and domain databases

InterPro

IPR016411. Helix_destablising_prot.
IPR012340. NA-bd_OB-fold.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.

PIRSF

PIRSF004311. Helix_destablz_SSB_T7. 1 hit.

ProtoNet

Search...

Entry information

Entry name

VHED_BPT7

Accession

Primary (citable) accession number: P03696

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 4, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)