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Reviewed, UniProtKB/Swiss-Prot P03692 (PRIM_BPT7)

Last modified June 16, 2009. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA primase/helicase
    EC=2.7.7.-
Gene names
Name: 4
OrganismEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifier10760 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae › T7-like viruses
Virus hostEscherichia coli [TaxID: 562]

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Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

DNA primase synthesizes RNA primers necessary for replication by DNA polymerase. It also functions as an ATP-dependent unwinding protein. Has a 5'->3' helicase activity; 4a also has a DNA primase activity.

Subunit structure

Homohexamer.

Sequence similarities

Contains 1 SF4 helicase domain.

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Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Chain a (identifier: P03692-1)

Also known as: 4a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Chain b (identifier: P03692-2)

Also known as: 4b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-63: Missing.
Note: No experimental confirmation available. The alternative initiation site Met-64 is uncertain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 566566DNA primase/helicase
PRO_0000003355

Regions

Domain281 – 548268SF4 helicase
Zinc finger17 – 3923C4-type; may recognize priming sites Potential
Nucleotide binding312 – 3198ATP Potential

Natural variations

Alternative sequence1 – 6363Missing in isoform Chain b.
VSP_018683

Secondary structure

1 ............................................................................................ 566
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform Chain a (4a) [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: C808C3663FEBE5AA

FASTA56662,655
        10         20         30         40         50         60 
MDNSHDSDSV FLYHIPCDNC GSSDGNSLFS DGHTFCYVCE KWTAGNEDTK ERASKRKPSG 

        70         80         90        100        110        120 
GKPMTYNVWN FGESNGRYSA LTARGISKET CQKAGYWIAK VDGVMYQVAD YRDQNGNIVS 

       130        140        150        160        170        180 
QKVRDKDKNF KTTGSHKSDA LFGKHLWNGG KKIVVTEGEI DMLTVMELQD CKYPVVSLGH 

       190        200        210        220        230        240 
GASAAKKTCA ANYEYFDQFE QIILMFDMDE AGRKAVEEAA QVLPAGKVRV AVLPCKDANE 

       250        260        270        280        290        300 
CHLNGHDREI MEQVWNAGPW IPDGVVSALS LRERIREHLS SEESVGLLFS GCTGINDKTL 

       310        320        330        340        350        360 
GARGGEVIMV TSGSGMGKST FVRQQALQWG TAMGKKVGLA MLEESVEETA EDLIGLHNRV 

       370        380        390        400        410        420 
RLRQSDSLKR EIIENGKFDQ WFDELFGNDT FHLYDSFAEA ETDRLLAKLA YMRSGLGCDV 

       430        440        450        460        470        480 
IILDHISIVV SASGESDERK MIDNLMTKLK GFAKSTGVVL VVICHLKNPD KGKAHEEGRP 

       490        500        510        520        530        540 
VSITDLRGSG ALRQLSDTII ALERNQQGDM PNLVLVRILK CRFTGDTGIA GYMEYNKETG 

       550        560 
WLEPSSYSGE EESHSESTDW SNDTDF

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Isoform Chain b (4b).

Checksum: 402AD68B32328F71
Show »

FASTA50355,743

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References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements."
Dunn J.J., Studier F.W.
J. Mol. Biol. 166:477-535(1983) [PubMed: 6864790] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4."
Dunn J.J., Studier F.W.
J. Mol. Biol. 148:303-330(1981) [PubMed: 7310871] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-179.
[3]"Characterization and crystallization of the helicase domain of bacteriophage T7 gene 4 protein."
Bird L.E., Haekansson K., Pan H., Wigley D.B.
Nucleic Acids Res. 25:2620-2626(1997) [PubMed: 9185573] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7."
Sawaya M.R., Guo S., Tabor S., Richardson C.C., Ellenberger T.
Cell 99:167-177(1999) [PubMed: 10535735] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 271-566.
[5]"Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides."
Singleton M.R., Sawaya M.R., Ellenberger T., Wigley D.B.
Cell 101:589-600(2000) [PubMed: 10892646] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 261-549.
+Additional computationally mapped references.

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Cross-references

Sequence databases

V01127 Genomic DNA. Translation: CAA24348.1.
V01146 Genomic DNA. Translation: CAA24405.1.
V01146 Genomic DNA. Translation: CAA24407.1. Different initiation.
PIRYDBPA7. A04314.
RefSeqNP_041975.1.
NP_041977.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1CR0X-ray2.30A272-566[»]
1CR1X-ray2.30A272-566[»]
1CR2X-ray2.30A272-566[»]
1CR4X-ray2.50A272-566[»]
1E0JX-ray3.00A/B/C/D/E/F261-549[»]
1E0KX-ray3.30A/B/C/D/E/F261-549[»]
1NUIX-ray2.90A/B1-255[»]
1Q57X-ray3.45A/B/C/D/E/F/G64-566[»]
ModBaseSearch...

Genome annotation databases

GeneID1261046.
1261048.
GenomeReviewsGene locus 4 in contig V01146_GR.

Family and domain databases

InterProIPR013237. BPP4_alpha_Zn_bd.
IPR007694. DNA_helicase_DnaB-like_C.
IPR006171. Toprim_domain.
IPR006154. Toprim_sub.
[Graphical view]
PfamPF08273. Prim_Zn_Ribbon. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
ProDomPD332834. DnaB_C. 1 hit.
PD002276. Toprim_primase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00778. Prim_Zn_Ribbon. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
PROSITEPS51199. SF4_HELICASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePRIM_BPT7
AccessionPrimary (citable) accession number: P03692
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 16, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents