P03681 (TERM_BPPH2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA terminal protein

Alternative name(s):
Protein Gp3

Gene names

Name:

3

Organism

Bacillus phage phi29 (Bacteriophage phi-29)

Taxonomic identifier

10756 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Picovirinae › Phi29likevirus

Virus host

Bacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

DNA terminal protein is linked to the 5'-ends of both strands of the genome through a phosphodiester bond between the beta-hydroxyl group of a serine residue and the 5'-phosphate of the terminal deoxyadenylate. This protein is essential for DNA replication and is involved in the priming of DNA elongation.

Ontologies

Keywords
Biological process	DNA replication
Developmental stage	Early protein
PTM	Covalent protein-DNA linkage Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: InterPro protein-DNA covalent cross-linking Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 266

266

DNA terminal protein

PRO_0000106556

Regions

Region

256 – 258

3

Interaction with the viral DNA polymerase

Amino acid modifications

Modified residue

232

1

O-(5'-phospho-DNA)-serine

Natural variations

Natural variant

124 – 266

143

Missing in mutant SUS3(91).

Secondary structure

1

.

.

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.

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.

.

.

266

Helix Strand Turn

Details...

Beta strand

77 – 79

3

Beta strand

85 – 87

3

Helix

88 – 112

25

Helix

115 – 118

4

Helix

150 – 152

3

Helix

156 – 168

13

Helix

174 – 194

21

Helix

198 – 208

11

Helix

212 – 222

11

Turn

223 – 225

3

Helix

237 – 255

19

Sequences

	Sequence		Length	Mass (Da)
	P03681 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 4C87B55670A48D65	FASTA	266	31,049
	10 20 30 40 50 60 MARSPRIRIK DNDKAEYARL VKNTKAKIAR TKKKYGVDLT AEIDIPDLDS FETRAQFNKW 70 80 90 100 110 120 KEQASSFTNR ANMRYQFEKN AYGVVASKAK IAEIERNTKE VQRLVDEKIK AMKDKEYYAG 130 140 150 160 170 180 GKPQGTIEQR IAMTSPAHVT GINRPHDFDF SKVRSYSRLR TLEESMEMRT DPQYYEKKMI 190 200 210 220 230 240 QLQLNFIKSV EGSFNSFDAA DELIEELKKI PPDDFYELFL RISEISFEEF DSEGNTVENV 250 260 EGNVYKILSY LEQYRRGDFD LSLKGF

References

[1]	"Nucleotide sequence of the early genes 3 and 4 of bacteriophage phi 29." Escarmis C., Salas M. Nucleic Acids Res. 10:5785-5798(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the major early region of bacteriophage phi 29." Yoshikawa H., Ito J. Gene 17:323-335(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Protein p3 is linked to the DNA of phage phi 29 through a phosphoester bond between serine and 5'-dAMP." Hermoso J.M., Salas M. Proc. Natl. Acad. Sci. U.S.A. 77:6425-6428(1980) [PubMed] [Europe PMC] [Abstract] Cited for: COVALENT DNA LINKAGE TO SER-232.
[4]	"The RGD sequence in phage phi29 terminal protein is required for interaction with phi29 DNA polymerase." Illana B., Zaballos A., Blanco L., Salas M. Virology 248:12-19(1998) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF ARG-256; GLY-257 AND ASP-258.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V01155 Genomic DNA. Translation: CAA24481.1.
J02479 Genomic DNA. Translation: AAA32290.1.

PIR

ERBP39. A93439.

RefSeq

YP_002004530.1. NC_011048.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EX3	X-ray	3.00	B/D/F/H/J/L	71-266	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

6446514.

Phylogenomic databases

ProtClustDB

PHA0490.

Family and domain databases

InterPro

IPR008770. DNA_terminal_Gp3.
[Graphical view]

Pfam

PF05435. Phi-29_GP3. 1 hit.
[Graphical view]

PIRSF

PIRSF004179. Phi-29_GP3. 1 hit.

ProDom

PD013906. Phi-29_GP3. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

EvolutionaryTrace

P03681.

Entry information

Entry name

TERM_BPPH2

Accession

Primary (citable) accession number: P03681

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program