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Protein

Virion export protein

Gene

IV

Organism
Enterobacteria phage f1 (Bacteriophage f1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts in the assembly and extrusion of the bacteriophage by forming a channel across the host outer membrane. This channel is just large enough to allow a newly synthesized phage particle to pass through. Extrusion is a process of concomitant assembly and secretion and takes place at specific assembly sites where host inner and outer membranes are in close contacts.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Viral extrusion, Virus exit from host cell

Protein family/group databases

TCDBi1.B.22.5.1. the outer bacterial membrane secretin (secretin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Virion export protein
Alternative name(s):
Gene 4 protein
Short name:
G4P
Gene namesi
Name:IV
OrganismiEnterobacteria phage f1 (Bacteriophage f1)
Taxonomic identifieri10863 [NCBI]
Taxonomic lineageiVirusesssDNA virusesInoviridaeInovirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000002557 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei317 – 33721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 426405Virion export proteinPRO_0000209448Add
BLAST

Interactioni

Subunit structurei

Homomultimer. The channel is composed of 14 G4P subunits that confer a barrel-like structure. Interacts with G1P; this interaction results in a complex that spans the inner an outer host membranes (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the inovirus G4P protein family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR001775. GspD/PilQ.
IPR004846. T2SS/T3SS.
IPR004845. T2SS_GspD_CS.
[Graphical view]
PfamiPF00263. Secretin. 1 hit.
[Graphical view]
PRINTSiPR00811. BCTERIALGSPD.
PROSITEiPS00875. T2SP_D. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLNVINFV FLMFVSSSSF AQVIEMNNSS LRDFVTWYSK QTGESVIVSP
60 70 80 90 100
DVKGTVTVYS SDVKPENLRD FFISVLRANN FDMVGSIPSI IQKYNPNNQD
110 120 130 140 150
YIDELPSSDN QEYDDNSAPS GGFFVPQNDN VTQTFKINNV RAKDLIRVVE
160 170 180 190 200
LFVKSNTSKS SNVLSVDGSN LLVVSAPKDI LDNLPQFLST VDLPTDQILI
210 220 230 240 250
EGLIFEVQQG DALDFSFAAG SQRGTVAGGV NTDRLTSVLS SAGGSFGIFN
260 270 280 290 300
GDVLGLSVRA LKTNSHSKIL SVPRILTLSG QKGSISVGQN VPFITGRVTG
310 320 330 340 350
ESANVNNPFQ TVERQNVGIS MSVFPVAMAG GNIVLDITSK ADSLSSSTQA
360 370 380 390 400
SDVITNQRSI ATTVNLRDGQ TLLLGGLTDY KNTSQDSGVP FLSKIPLIGL
410 420
LFSSRSDSNE ESTLYVLVKA TIVRAL
Length:426
Mass (Da):45,842
Last modified:July 21, 1986 - v1
Checksum:i27593CC35BAE610B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301S → P in AAA32218 (PubMed:6292494).Curated
Sequence conflicti70 – 701D → N in AAA32218 (PubMed:6292494).Curated
Sequence conflicti87 – 871I → N in AAA32218 (PubMed:6292494).Curated
Sequence conflicti166 – 1661V → I in AAA32218 (PubMed:6292494).Curated
Sequence conflicti312 – 3121V → I in AAA32218 (PubMed:6292494).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00606 Genomic DNA. Translation: CAA23875.1.
J02448 Genomic DNA. Translation: AAA32218.1.
M10641 Genomic DNA. Translation: AAA32222.1.
M38348 Genomic DNA. Translation: AAA32221.1. Sequence problems.
PIRiC04268. Z4BPF1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00606 Genomic DNA. Translation: CAA23875.1.
J02448 Genomic DNA. Translation: AAA32218.1.
M10641 Genomic DNA. Translation: AAA32222.1.
M38348 Genomic DNA. Translation: AAA32221.1. Sequence problems.
PIRiC04268. Z4BPF1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.B.22.5.1. the outer bacterial membrane secretin (secretin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001775. GspD/PilQ.
IPR004846. T2SS/T3SS.
IPR004845. T2SS_GspD_CS.
[Graphical view]
PfamiPF00263. Secretin. 1 hit.
[Graphical view]
PRINTSiPR00811. BCTERIALGSPD.
PROSITEiPS00875. T2SP_D. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence and genome organisation of filamentous bacteriophages f1 and fd."
    Beck E., Zink B.
    Gene 16:35-58(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of bacteriophage f1 DNA."
    Hill D.F., Petersen G.B.
    J. Virol. 44:32-46(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "DNA sequence analysis of the defective interfering particles of bacteriophage f1."
    Ravetch J.V., Horiuchi K., Zinder N.D.
    J. Mol. Biol. 128:305-318(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 376-426.
  4. "Nucleotide sequence of a gene IV fragment of bacteriophage f1."
    Grachev S.A., Kolosov M.N., Korobko V.G., Petrov N.A.
    Bioorg. Khim. 4:569-570(1978)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 341-399.
  5. "A trans-envelope protein complex needed for filamentous phage assembly and export."
    Feng J.N., Model P., Russel M.
    Mol. Microbiol. 34:745-755(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH G1P.

Entry informationi

Entry nameiG4P_BPF1
AccessioniPrimary (citable) accession number: P03666
Secondary accession number(s): Q96223
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 11, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.