P03633 (B_BPPHX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Internal scaffolding protein B
Short name=GPB

Gene names

Name:

B

Organism

Enterobacteria phage phiX174 (Bacteriophage phi-X174)

Taxonomic identifier

10847 [NCBI]

Taxonomic lineage

Viruses › ssDNA viruses › Microviridae › Microvirus

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in the assembly of the viral procapsid in the cytoplasm. Forms first a 12S pre-assembly complex with protein H, and F and G pentamers, then twelve 12S complexes are joined by the D protein to form the procapsid. Internal scaffold protein B is released from the procapsid upon genome packaging, possibly through affinity displacement by the protein J, or by proteolysis. Ref.2
Subunit structure	Component of the procapsid complex composed of 60 copies of the internally located B, 240 copies of the external scaffolding protein D, 60 copies of each of the viral structural proteins F and G proteins, and 12 copies of H.
Subcellular location	Host cytoplasm.
Post-translational modification	The proteolytic cleavage of the protein B by the host may occur directly inside the procapsid, thus releasing the scaffold protein in order to continue virion assembly. The B protein would be completely processed into small fragments by proteolytic digestion.
Sequence similarities	Belongs to the microviridae B protein family.

Ontologies

Keywords
Biological process	Capsid assembly
Cellular component	Host cytoplasm
Technical term	3D-structure
Gene Ontology (GO)
Biological process	viral capsid assembly Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell viral procapsid Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 120

120

Internal scaffolding protein B

PRO_0000164870

Sites

Site

76 – 77

2

Cleavage; by host

Secondary structure

1

.

.

.

.

.

120

Helix Strand Turn

Details...

Helix

87 – 90

4

Helix

112 – 115

4

Sequences

	Sequence		Length	Mass (Da)
	P03633 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: D8E52894116497ED	FASTA	120	13,843
	10 20 30 40 50 60 MEQLTKNQAV ATSQEAVQNQ NEPQLRDENA HNDKSVHGVL NPTYQAGLRR DAVQPDIEAE 70 80 90 100 110 120 RKKRDEIEAG KSYCSRRFGG ATCDDKSAQI YARFDKNDWR IQPAEFYRFH DAEVNTFGYF

References

[1]	"Nucleotide sequence of bacteriophage phi X174 DNA." Sanger F., Air G.M., Barrell B.G., Brown N.L., Coulson A.R., Fiddes J.C., Hutchison C.A. III, Slocombe P.M., Smith M. Nature 265:687-695(1977) [PubMed: 870828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation and identification of bacteriophage phi X174 prohead." Mukai R., Hamatake R.K., Hayashi M. Proc. Natl. Acad. Sci. U.S.A. 76:4877-4881(1979) [PubMed: 159449] [Abstract] Cited for: FUNCTION AND PROCAPSID STRUCTURE.
[3]	"Proteolysis of bacteriophage phi X174 prohead protein gpB by a protease located in the Escherichia coli outer membrane." Richardson D.L. Jr., Aoyama A., Hayashi M. J. Bacteriol. 170:5564-5571(1988) [PubMed: 2973457] [Abstract] Cited for: CLEAVAGE SITE, PROTEIN SEQUENCING 1-5.
[4]	"Structure of a viral procapsid with molecular scaffolding." Dokland T., McKenna R., Ilag L.L., Bowman B.R., Incardona N.L., Fane B.A., Rossmann M.G. Nature 389:308-313(1997) [PubMed: 9305849] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02482 Genomic DNA. Translation: AAA32572.1.

PIR

ZBBPF4. A04241.

RefSeq

NP_040705.1. NC_001422.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AL0	X-ray	3.50	B	1-120	[»]
1CD3	X-ray	3.50	B	1-120	[»]

ProteinModelPortal

P03633.

SMR

P03633. Positions 80-120.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2546405.

GenomeReviews

Gene locus phiX174p04 in contig J02482_GR.

Phylogenomic databases

ProtClustDB

PHA0003.

Family and domain databases

InterPro

IPR003513. Phage_B.
[Graphical view]

Pfam

PF02304. Phage_B. 1 hit.
[Graphical view]

ProDom

PD012751. Phage_B. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

B_BPPHX

Accession

Primary (citable) accession number: P03633

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program