ID   CAPSD_BPPF1             Reviewed;          82 AA.
AC   P03621;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 2.
DT   22-FEB-2023, entry version 122.
DE   RecName: Full=Capsid protein G8P;
DE   AltName: Full=Coat protein B;
DE   AltName: Full=Gene 8 protein;
DE            Short=G8P;
DE   AltName: Full=Major coat protein;
DE   Flags: Precursor;
GN   Name=VIII;
OS   Pseudomonas phage Pf1 (Bacteriophage Pf1).
OC   Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC   Tubulavirales; Inoviridae; Primolicivirus.
OX   NCBI_TaxID=2011081;
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 25102-B1 / pf;
RX   PubMed=2010913; DOI=10.1016/0022-2836(91)90717-k;
RA   Hill D.F., Short N.J., Perham R.N., Petersen G.B.;
RT   "DNA sequence of the filamentous bacteriophage Pf1.";
RL   J. Mol. Biol. 218:349-364(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 37-82.
RX   PubMed=1110754; DOI=10.1038/253068a0;
RA   Nakashima Y., Wiseman R.L., Konigsberg W., Marvin D.A.;
RT   "Primary structure and sidechain interactions of Pf1 filamentous bacterial
RT   virus coat protein.";
RL   Nature 253:68-71(1975).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX   PubMed=8036516; DOI=10.1126/science.8036516;
RA   Liu D.J., Day L.A.;
RT   "Pf1 virus structure: helical coat protein and DNA with paraxial
RT   phosphates.";
RL   Science 265:671-674(1994).
RN   [4]
RP   STRUCTURE BY NMR.
RX   PubMed=1925543; DOI=10.1126/science.1925543;
RA   Nambudripad R., Stark W., Opella S.J., Makowski L.;
RT   "Membrane-mediated assembly of filamentous bacteriophage Pf1 coat
RT   protein.";
RL   Science 252:1305-1308(1991).
CC   -!- FUNCTION: Self assembles to form a helical capsid wrapping up the viral
CC       genomic DNA. The capsid displays a filamentous structure with a length
CC       of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding
CC       take place at the host inner membrane (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimerizes. There are several thousands of this protein
CC       in the phage capsid (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host membrane; Single-pass
CC       type I membrane protein. Note=prior to assembly, the major capsid
CC       protein is found associated with the bacterial host inner membrane.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the inovirus capsid protein family.
CC       {ECO:0000305}.
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DR   EMBL; X52107; CAA36331.1; -; Genomic_DNA.
DR   PIR; A04229; VCBPPF.
DR   PIR; S15143; S15143.
DR   PDB; 1IFM; Fiber; 3.30 A; A=37-82.
DR   PDB; 1IFN; Fiber; 4.00 A; A=37-82.
DR   PDB; 1PFI; Fiber; 3.00 A; A/B=37-82.
DR   PDB; 1PJF; NMR; -; A=37-82.
DR   PDB; 1QL1; Fiber; 3.10 A; A=37-82.
DR   PDB; 1QL2; Fiber; 3.10 A; A/B/C=37-82.
DR   PDB; 1ZN5; NMR; -; A=37-82.
DR   PDB; 2IFM; Fiber; 3.30 A; A=37-82.
DR   PDB; 2IFN; Fiber; 4.00 A; A=37-82.
DR   PDB; 2KLV; NMR; -; A=37-82.
DR   PDB; 2KSJ; Other; -; A=37-82.
DR   PDB; 2XKM; Other; 3.30 A; A=37-82.
DR   PDB; 3IFM; Fiber; 3.30 A; A=37-82.
DR   PDB; 4IFM; Fiber; 3.30 A; A=37-82.
DR   PDB; 6TUP; EM; 3.20 A; A=37-82.
DR   PDB; 6TUQ; EM; 3.90 A; A=37-82.
DR   PDBsum; 1IFM; -.
DR   PDBsum; 1IFN; -.
DR   PDBsum; 1PFI; -.
DR   PDBsum; 1PJF; -.
DR   PDBsum; 1QL1; -.
DR   PDBsum; 1QL2; -.
DR   PDBsum; 1ZN5; -.
DR   PDBsum; 2IFM; -.
DR   PDBsum; 2IFN; -.
DR   PDBsum; 2KLV; -.
DR   PDBsum; 2KSJ; -.
DR   PDBsum; 2XKM; -.
DR   PDBsum; 3IFM; -.
DR   PDBsum; 4IFM; -.
DR   PDBsum; 6TUP; -.
DR   PDBsum; 6TUQ; -.
DR   BMRB; P03621; -.
DR   SMR; P03621; -.
DR   EvolutionaryTrace; P03621; -.
DR   Proteomes; UP000002121; Genome.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.5.230; -; 1.
DR   InterPro; IPR008020; G8P.
DR   Pfam; PF05356; Phage_Coat_B; 1.
DR   SUPFAM; SSF57987; Inovirus (filamentous phage) major coat protein; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Capsid protein; Direct protein sequencing;
KW   Helical capsid protein; Host membrane; Membrane; Reference proteome;
KW   Signal; Transmembrane; Transmembrane helix; Virion.
FT   SIGNAL          1..28
FT   CHAIN           29..82
FT                   /note="Capsid protein G8P"
FT                   /id="PRO_0000003302"
FT   TOPO_DOM        29..57
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT   TOPO_DOM        79..82
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        45
FT                   /note="E -> Q (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   HELIX           39..51
FT                   /evidence="ECO:0007829|PDB:1PFI"
FT   HELIX           53..81
FT                   /evidence="ECO:0007829|PDB:1PFI"
SQ   SEQUENCE   82 AA;  8377 MW;  25FD055DA4661504 CRC64;
     MKAMKQRIAK FSPVASFRNL CIAGSVTAAT SLPAFAGVID TSAVESAITD GQGDMKAIGG
     YIVGALVILA VAGLIYSMLR KA
//