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P03621

- CAPSD_BPPF1

UniProt

P03621 - CAPSD_BPPF1

Protein

Capsid protein G8P

Gene

VIII

Organism
Pseudomonas phage Pf1 (Bacteriophage Pf1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane By similarity.By similarity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Capsid protein G8P
    Alternative name(s):
    Coat protein B
    Gene 8 protein
    Short name:
    G8P
    Major coat protein
    Gene namesi
    Name:VIII
    OrganismiPseudomonas phage Pf1 (Bacteriophage Pf1)
    Taxonomic identifieri10871 [NCBI]
    Taxonomic lineageiVirusesssDNA virusesInoviridaeInovirus
    Virus hostiPseudomonas aeruginosa [TaxID: 287]
    ProteomesiUP000002121: Genome

    Subcellular locationi

    Virion Curated. Host membrane; Single-pass type I membrane protein
    Note: prior to assembly, the major capsid protein is found associated with the bacterial host inner membrane.By similarity

    GO - Cellular componenti

    1. helical viral capsid Source: UniProtKB-KW
    2. host cell membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Helical capsid protein, Host membrane, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Add
    BLAST
    Chaini29 – 8254Capsid protein G8PPRO_0000003302Add
    BLAST

    Interactioni

    Subunit structurei

    Homomultimerizes. There are several thousands of this protein in the phage capsid By similarity.By similarity

    Structurei

    Secondary structure

    1
    82
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi39 – 5113
    Helixi53 – 8129

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IFMfiber diffraction3.30A37-82[»]
    1IFNfiber diffraction4.00A37-82[»]
    1PFIfiber diffraction3.00A/B37-82[»]
    1PJFNMR-A37-82[»]
    1QL1fiber diffraction3.10A37-82[»]
    1QL2fiber diffraction3.10A/B/C37-82[»]
    1ZN5NMR-A37-82[»]
    2IFMfiber diffraction3.30A37-82[»]
    2IFNfiber diffraction4.00A37-82[»]
    2KLVNMR-A37-82[»]
    2KSJOther-A37-82[»]
    2XKMOther3.30A37-82[»]
    3IFMfiber diffraction3.30A37-82[»]
    4IFMfiber diffraction3.30A37-82[»]
    ProteinModelPortaliP03621.
    SMRiP03621. Positions 37-82.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03621.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini29 – 5729PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini79 – 824CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei58 – 7821HelicalAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the inovirus capsid protein family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR008020. Major_coat_inovirus.
    [Graphical view]
    PfamiPF05356. Phage_Coat_B. 1 hit.
    [Graphical view]
    ProDomiPD059964. Phage_coat_B. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P03621-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKAMKQRIAK FSPVASFRNL CIAGSVTAAT SLPAFAGVID TSAVESAITD   50
    GQGDMKAIGG YIVGALVILA VAGLIYSMLR KA 82
    Length:82
    Mass (Da):8,377
    Last modified:May 1, 1992 - v2
    Checksum:i25FD055DA4661504
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 451E → Q AA sequence (PubMed:1110754)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52107 Genomic DNA. Translation: CAA36331.1.
    PIRiA04229. VCBPPF.
    S15143.
    RefSeqiNP_039603.1. NC_001331.1.

    Genome annotation databases

    GeneIDi1260711.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52107 Genomic DNA. Translation: CAA36331.1 .
    PIRi A04229. VCBPPF.
    S15143.
    RefSeqi NP_039603.1. NC_001331.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IFM fiber diffraction 3.30 A 37-82 [» ]
    1IFN fiber diffraction 4.00 A 37-82 [» ]
    1PFI fiber diffraction 3.00 A/B 37-82 [» ]
    1PJF NMR - A 37-82 [» ]
    1QL1 fiber diffraction 3.10 A 37-82 [» ]
    1QL2 fiber diffraction 3.10 A/B/C 37-82 [» ]
    1ZN5 NMR - A 37-82 [» ]
    2IFM fiber diffraction 3.30 A 37-82 [» ]
    2IFN fiber diffraction 4.00 A 37-82 [» ]
    2KLV NMR - A 37-82 [» ]
    2KSJ Other - A 37-82 [» ]
    2XKM Other 3.30 A 37-82 [» ]
    3IFM fiber diffraction 3.30 A 37-82 [» ]
    4IFM fiber diffraction 3.30 A 37-82 [» ]
    ProteinModelPortali P03621.
    SMRi P03621. Positions 37-82.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1260711.

    Miscellaneous databases

    EvolutionaryTracei P03621.

    Family and domain databases

    InterProi IPR008020. Major_coat_inovirus.
    [Graphical view ]
    Pfami PF05356. Phage_Coat_B. 1 hit.
    [Graphical view ]
    ProDomi PD059964. Phage_coat_B. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequence of the filamentous bacteriophage Pf1."
      Hill D.F., Short N.J., Perham R.N., Petersen G.B.
      J. Mol. Biol. 218:349-364(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 25102-B1 / pf.
    2. "Primary structure and sidechain interactions of Pf1 filamentous bacterial virus coat protein."
      Nakashima Y., Wiseman R.L., Konigsberg W., Marvin D.A.
      Nature 253:68-71(1975) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 37-82.
    3. "Pf1 virus structure: helical coat protein and DNA with paraxial phosphates."
      Liu D.J., Day L.A.
      Science 265:671-674(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
    4. "Membrane-mediated assembly of filamentous bacteriophage Pf1 coat protein."
      Nambudripad R., Stark W., Opella S.J., Makowski L.
      Science 252:1305-1308(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiCAPSD_BPPF1
    AccessioniPrimary (citable) accession number: P03621
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3