Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P03621

- CAPSD_BPPF1

UniProt

P03621 - CAPSD_BPPF1

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Capsid protein G8P

Gene

VIII

Organism
Pseudomonas phage Pf1 (Bacteriophage Pf1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane By similarity.By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid protein G8P
Alternative name(s):
Coat protein B
Gene 8 protein
Short name:
G8P
Major coat protein
Gene namesi
Name:VIII
OrganismiPseudomonas phage Pf1 (Bacteriophage Pf1)
Taxonomic identifieri10871 [NCBI]
Taxonomic lineageiVirusesssDNA virusesInoviridaeInovirus
Virus hostiPseudomonas aeruginosa [TaxID: 287]
ProteomesiUP000002121: Genome

Subcellular locationi

Virion Curated. Host membrane; Single-pass type I membrane protein
Note: prior to assembly, the major capsid protein is found associated with the bacterial host inner membrane.By similarity

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Add
BLAST
Chaini29 – 8254Capsid protein G8PPRO_0000003302Add
BLAST

Interactioni

Subunit structurei

Homomultimerizes. There are several thousands of this protein in the phage capsid By similarity.By similarity

Structurei

Secondary structure

1
82
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi39 – 5113
Helixi53 – 8129

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IFMfiber diffraction3.30A37-82[»]
1IFNfiber diffraction4.00A37-82[»]
1PFIfiber diffraction3.00A/B37-82[»]
1PJFNMR-A37-82[»]
1QL1fiber diffraction3.10A37-82[»]
1QL2fiber diffraction3.10A/B/C37-82[»]
1ZN5NMR-A37-82[»]
2IFMfiber diffraction3.30A37-82[»]
2IFNfiber diffraction4.00A37-82[»]
2KLVNMR-A37-82[»]
2KSJOther-A37-82[»]
2XKMOther3.30A37-82[»]
3IFMfiber diffraction3.30A37-82[»]
4IFMfiber diffraction3.30A37-82[»]
ProteinModelPortaliP03621.
SMRiP03621. Positions 37-82.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03621.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 5729PeriplasmicSequence AnalysisAdd
BLAST
Topological domaini79 – 824CytoplasmicSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei58 – 7821HelicalAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the inovirus capsid protein family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008020. Major_coat_inovirus.
[Graphical view]
PfamiPF05356. Phage_Coat_B. 1 hit.
[Graphical view]
ProDomiPD059964. Phage_coat_B. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03621-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKAMKQRIAK FSPVASFRNL CIAGSVTAAT SLPAFAGVID TSAVESAITD
60 70 80
GQGDMKAIGG YIVGALVILA VAGLIYSMLR KA
Length:82
Mass (Da):8,377
Last modified:May 1, 1992 - v2
Checksum:i25FD055DA4661504
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451E → Q AA sequence (PubMed:1110754)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52107 Genomic DNA. Translation: CAA36331.1.
PIRiA04229. VCBPPF.
S15143.
RefSeqiNP_039603.1. NC_001331.1.

Genome annotation databases

GeneIDi1260711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52107 Genomic DNA. Translation: CAA36331.1 .
PIRi A04229. VCBPPF.
S15143.
RefSeqi NP_039603.1. NC_001331.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IFM fiber diffraction 3.30 A 37-82 [» ]
1IFN fiber diffraction 4.00 A 37-82 [» ]
1PFI fiber diffraction 3.00 A/B 37-82 [» ]
1PJF NMR - A 37-82 [» ]
1QL1 fiber diffraction 3.10 A 37-82 [» ]
1QL2 fiber diffraction 3.10 A/B/C 37-82 [» ]
1ZN5 NMR - A 37-82 [» ]
2IFM fiber diffraction 3.30 A 37-82 [» ]
2IFN fiber diffraction 4.00 A 37-82 [» ]
2KLV NMR - A 37-82 [» ]
2KSJ Other - A 37-82 [» ]
2XKM Other 3.30 A 37-82 [» ]
3IFM fiber diffraction 3.30 A 37-82 [» ]
4IFM fiber diffraction 3.30 A 37-82 [» ]
ProteinModelPortali P03621.
SMRi P03621. Positions 37-82.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1260711.

Miscellaneous databases

EvolutionaryTracei P03621.

Family and domain databases

InterProi IPR008020. Major_coat_inovirus.
[Graphical view ]
Pfami PF05356. Phage_Coat_B. 1 hit.
[Graphical view ]
ProDomi PD059964. Phage_coat_B. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "DNA sequence of the filamentous bacteriophage Pf1."
    Hill D.F., Short N.J., Perham R.N., Petersen G.B.
    J. Mol. Biol. 218:349-364(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25102-B1 / pf.
  2. "Primary structure and sidechain interactions of Pf1 filamentous bacterial virus coat protein."
    Nakashima Y., Wiseman R.L., Konigsberg W., Marvin D.A.
    Nature 253:68-71(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 37-82.
  3. "Pf1 virus structure: helical coat protein and DNA with paraxial phosphates."
    Liu D.J., Day L.A.
    Science 265:671-674(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
  4. "Membrane-mediated assembly of filamentous bacteriophage Pf1 coat protein."
    Nambudripad R., Stark W., Opella S.J., Makowski L.
    Science 252:1305-1308(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiCAPSD_BPPF1
AccessioniPrimary (citable) accession number: P03621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 1, 1992
Last modified: October 29, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3