P03594 (RDRP_BMV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase 2a
Short name=protein 2a
EC=2.7.7.48

Gene names

ORF Names:

ORF2a

Organism

Brome mosaic virus (BMV)

Taxonomic identifier

12302 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Bromovirus

Virus host

Bromus inermis (Smooth brome grass) (Bromopsis inermis) [TaxID: 15371]

Protein attributes

Sequence length	822 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-dependent RNA polymerase which replicates the viral genome composed of 3 RNA segments, RNA1, RNA2 and RNA3 Potential.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Interacts with replication protein 1a. Ref.2
Sequence similarities	Belongs to the ssRNA positive-strand viruses RNA-directed RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW endodeoxyribonuclease activity, producing 5'-phosphomonoesters Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 822	822	RNA-directed RNA polymerase 2a		PRO_0000083269
Regions
	Domain	459 – 572	114	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P03594 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: C13B99016B2C54AC	FASTA	822	93,927
	10 20 30 40 50 60 MSSKTWDDDF VRQVPSFQWI IDQSLEDEVE AASLQVQEPA DGVAIDGSLA SFKLAIAPLE 70 80 90 100 110 120 IGGVFDPPFD RVRWGSICDT VQQMVQQFTD RPLIPQAEMA RMLYLDIPGS FVLEDEIDDW 130 140 150 160 170 180 YPEDTSDGYG VSFAADEDHA SDLKLASDSS NCEIEEVRVT GDTPKELTLG DRYMGIDEEF 190 200 210 220 230 240 QTTNTDYDIT LQIMNPIEHR VSRVIDTHCH PDNPDISTGP IYMERVSLAR TEATSHSILP 250 260 270 280 290 300 THAYFDDSYH QALVENGDYS MDFDRIRLKQ SDVDWYRDPD KYFQPKMNIG SAQRRVGTQK 310 320 330 340 350 360 EVLTALKKRN ADVPEMGDAI NMKDTAKAIA KRFRSTFLNV DGEDCLRASM DVMTKCLEYH 370 380 390 400 410 420 KKWGKHMDLQ GVNVAAETDL CRYQHMLKSD VKPVVTDTLH LERAVAATIT FHSKGVTSNF 430 440 450 460 470 480 SPFFTACFEK LSLALKSRFI VPIGKISSLE LKNVRLNNRY FLEADLSKFD KSQGELHLEF 490 500 510 520 530 540 QREILLALGF PAPLTNWWSD FHRDSYLSDP HAKVGMSVSF QRRTGDAFTY FGNTLVTMAM 550 560 570 580 590 600 IAYASDLSDC DCAIFSGDDS LIISKVKPVL DTDMFTSLFN MEIKVMDPSV PYVCSKFLVE 610 620 630 640 650 660 TEMGNLVSVP DPLREIQRLA KRKILRDEQM LRAHFVSFCD RMKFINQLDE KMITTLCHFV 670 680 690 700 710 720 YLKYGKEKPW IFEEVRAALA AFSLYSENFL RFSDCYCTEG IRVYQMSDPV CKFKRTTEER 730 740 750 760 770 780 KTDGDWFHNW KNPKFPGVLD KVYRTIGIYS SDCSTKELPV KRIGRLHEAL ERESLKLAND 790 800 810 820 RRTTQRLKKK VDDYATGRGG LTSVDALLVK SHCETFKPSD LR

References

[1]	"Nucleotide sequence of the brome mosaic virus genome and its implications for viral replication." Ahlquist P., Dasgupta R., Kaesberg P. J. Mol. Biol. 172:369-383(1984) [PubMed: 6694215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"An amphipathic alpha-helix controls multiple roles of brome mosaic virus protein 1a in RNA replication complex assembly and function." Liu L., Westler W.M., den Boon J.A., Wang X., Diaz A., Steinberg H.A., Ahlquist P. PLoS Pathog. 5:E1000351-E1000351(2009) [PubMed: 19325881] [Abstract] Cited for: INTERACTION WITH REPLICATION PROTEIN 1A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01678 Unassigned RNA. Translation: CAA25834.1.
PIR	P2BVA. A04202.
RefSeq	NP_041197.1. NC_002027.1.
3D structure databases
ProteinModelPortal	P03594.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	962145.
Family and domain databases
InterPro	IPR007610. BBMV_Gp1_N. IPR018228. DNase_TatD-rel_CS. IPR007094. RNA-dir_pol_PSvirus. IPR001788. Tymovirus_RNA-dep_RNA_pol. [Graphical view]
Pfam	PF04522. DUF585. 1 hit. PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_BMV

Accession

Primary (citable) accession number: P03594

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	October 19, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program