Reviewed, UniProtKB/Swiss-Prot P03594 (ORF2A_BMV)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase 2A
      Short name=protein 2A
    EC=2.7.7.48

Gene names

Name:	RNA2
ORF Names:	ORF2a

Organism

Brome mosaic virus (BMV)

Taxonomic identifier

12302 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Bromovirus

Virus host

Bromus inermis (Smooth brome grass) [TaxID: 15371]

Protein attributes

Sequence length	822 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-directed RNA polymerase.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Miscellaneous	The virus has a tripartite genome (RNA1, RNA2 and RNA3) and an encapsidated subgenomic RNA (RNA4) from which the coat protein is expressed. Virions are icosahedral, not enveloped, with 32 capsomers per nucleocapsid.
Sequence similarities	Belongs to the cucumoviruses/bromoviruses 2A family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 822	822	RNA-directed RNA polymerase 2A		PRO_0000083269
Regions
	Domain	459 – 572	114	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P03594-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: C13B99016B2C54AC	FASTA	822	93,927
	10 20 30 40 50 60 MSSKTWDDDF VRQVPSFQWI IDQSLEDEVE AASLQVQEPA DGVAIDGSLA SFKLAIAPLE 70 80 90 100 110 120 IGGVFDPPFD RVRWGSICDT VQQMVQQFTD RPLIPQAEMA RMLYLDIPGS FVLEDEIDDW 130 140 150 160 170 180 YPEDTSDGYG VSFAADEDHA SDLKLASDSS NCEIEEVRVT GDTPKELTLG DRYMGIDEEF 190 200 210 220 230 240 QTTNTDYDIT LQIMNPIEHR VSRVIDTHCH PDNPDISTGP IYMERVSLAR TEATSHSILP 250 260 270 280 290 300 THAYFDDSYH QALVENGDYS MDFDRIRLKQ SDVDWYRDPD KYFQPKMNIG SAQRRVGTQK 310 320 330 340 350 360 EVLTALKKRN ADVPEMGDAI NMKDTAKAIA KRFRSTFLNV DGEDCLRASM DVMTKCLEYH 370 380 390 400 410 420 KKWGKHMDLQ GVNVAAETDL CRYQHMLKSD VKPVVTDTLH LERAVAATIT FHSKGVTSNF 430 440 450 460 470 480 SPFFTACFEK LSLALKSRFI VPIGKISSLE LKNVRLNNRY FLEADLSKFD KSQGELHLEF 490 500 510 520 530 540 QREILLALGF PAPLTNWWSD FHRDSYLSDP HAKVGMSVSF QRRTGDAFTY FGNTLVTMAM 550 560 570 580 590 600 IAYASDLSDC DCAIFSGDDS LIISKVKPVL DTDMFTSLFN MEIKVMDPSV PYVCSKFLVE 610 620 630 640 650 660 TEMGNLVSVP DPLREIQRLA KRKILRDEQM LRAHFVSFCD RMKFINQLDE KMITTLCHFV 670 680 690 700 710 720 YLKYGKEKPW IFEEVRAALA AFSLYSENFL RFSDCYCTEG IRVYQMSDPV CKFKRTTEER 730 740 750 760 770 780 KTDGDWFHNW KNPKFPGVLD KVYRTIGIYS SDCSTKELPV KRIGRLHEAL ERESLKLAND 790 800 810 820 RRTTQRLKKK VDDYATGRGG LTSVDALLVK SHCETFKPSD LR

References

[1]	"Nucleotide sequence of the brome mosaic virus genome and its implications for viral replication." Ahlquist P., Dasgupta R., Kaesberg P. J. Mol. Biol. 172:369-383(1984) [PubMed: 6694215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X01678 Unassigned RNA. Translation: CAA25834.1.
PIR	P2BVA. A04202.
RefSeq	NP_041197.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	962145.
Enzyme and pathway databases
BRENDA	2.7.7.48. 271343.
Family and domain databases
InterPro	IPR007610. DUF585. IPR001788. RNA-dep_RNA_pol_vir-typ. IPR007094. RNA_pol_PSvir. [Graphical view]
Pfam	PF04522. DUF585. 1 hit. PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ORF2A_BMV

Accession

Primary (citable) accession number: P03594

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)