P03593 (RDRP_AMVLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase 2a
Short name=protein 2a
EC=2.7.7.48

Gene names

ORF Names:

ORF2a

Organism

Alfalfa mosaic virus (strain 425 / isolate Leiden)

Taxonomic identifier

12322 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Alfamovirus

Virus host

Cicer arietinum (Chickpea) (Garbanzo) [TaxID: 3827]
Glycine max (Soybean) (Glycine hispida) [TaxID: 3847]
Lens culinaris (Lentil) (Cicer lens) [TaxID: 3864]
Lupinus [TaxID: 3869]
Medicago sativa (Alfalfa) [TaxID: 3879]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Pisum sativum (Garden pea) [TaxID: 3888]
Vigna radiata var. radiata (Mung bean) (Phaseolus aureus) [TaxID: 3916]
Vigna unguiculata (Cowpea) [TaxID: 3917]
Apium graveolens (Celery) [TaxID: 4045]
Capsicum annuum (Bell pepper) [TaxID: 4072]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Nicotiana tabacum (Common tobacco) [TaxID: 4097]
Solanum tuberosum (Potato) [TaxID: 4113]
Lactuca sativa (Garden lettuce) [TaxID: 4236]
Philadelphus [TaxID: 23113]
Lablab purpureus (Hyacinth bean) (Dolichos lab lab) [TaxID: 35936]
Caryopteris incana [TaxID: 41386]
Trifolium incarnatum (Crimson clover) [TaxID: 60916]
Astragalus glycyphyllos (Wild liquorice) [TaxID: 83862]
Viburnum opulus (Guelder-rose) [TaxID: 85293]
Malva parviflora (Little mallow) (Cheeseweed mallow) [TaxID: 145753]
Teramnus repens [TaxID: 157662]

Protein attributes

Sequence length	790 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which replicates the viral genome composed of 3 RNA segments, RNA1, RNA2 and RNA3 Potential.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Interacts with replication protein 1a By similarity.
Sequence similarities	Belongs to the bromoviridae 2a family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 790	790	RNA-directed RNA polymerase 2a		PRO_0000083267
Regions
	Domain	524 – 639	116	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P03593 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: B435C9EB9AA853C2	FASTA	790	89,855
	10 20 30 40 50 60 MFTLLRCLGF GVNEPTNTSS SEYVPEYSVE EISNEVAELD SVDPLFQCYK HVFVSLMLVR 70 80 90 100 110 120 KMTQAAEDFL ESFGGEFDSP CCRVYRLYRH FVNEDDAPAW AIPNVVNEDS YDDYAYLREE 130 140 150 160 170 180 LDAIDSSFEL LNEERELSEI TDRLNALRFF PVSKTEALPV ANVQEVKLIS ETYQLLMTFI 190 200 210 220 230 240 NYSDENIPSE MPAPLLDELG MLPEELGPLN EIEDIKPVAA PITLLSEFRA SDNAKPLDIV 250 260 270 280 290 300 EIIPDVSPTK PYEAVISGND WMTLGRIIPT TPVPTIRDVF FSGLSRHGSP EVIQNALDEF 310 320 330 340 350 360 LPLHHSIDDK YFQEWVETSD KSLDVDPCRI DLSVFNNWQS SENCYEPRFK TGALSTRKGT 370 380 390 400 410 420 QTEALLAIKK RNMNVPNLGQ IYDVNSVANS VVNKLLTTVI DPDKLCMFPD FISEGEVSYF 430 440 450 460 470 480 QDYIVGKNPD PELYSDPLGV RSIDSYKHMI KSVLKPVEDN SLHLERPMPA TITYHDKDIV 490 500 510 520 530 540 MSSSPIFLAA AARLMLILRD KITIPSGKFH QLFSIDAEAF DASFHFKEID FSKFDKSQNE 550 560 570 580 590 600 LHHLIQERFL KYLGIPNEFL TLWFNAHRKS RISDSKNGVF FNVDFQRRTG DALTYLGNTI 610 620 630 640 650 660 VTLACLCHVY DLMDPNVKFV VASGDDSLIG TVEELPRDQE FLFTTLFNLE AKFPHNQPFI 670 680 690 700 710 720 CSKFLITMPT TSGGKVVLPI PNPLKLLIRL GSKKVNADIF DEWYQSWIDI IGGFNDHHVI 730 740 750 760 770 780 RCVAAMTAHR YLRRPSLYLE AALESLGKIF AGKTLCKECL FNEKHESNVK IKPRRVKKSH 790 SDARSRARRA

References

[1]	"Complete nucleotide sequence of alfalfa mosaic virus RNA 2." Cornelissen B.J.C., Brederode F.T., Veeneman G.H., van Boom J.H., Bol J.F. Nucleic Acids Res. 11:3019-3025(1983) [PubMed: 6304618] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01572 Unassigned RNA. Translation: CAA25728.1.
PIR	WMFM9. A04201.
RefSeq	YP_053235.1. NC_002024.2.
3D structure databases
ProteinModelPortal	P03593.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	962668.
Family and domain databases
InterPro	IPR007094. RNA-dir_pol_PSvirus. IPR001788. Tymovirus_RNA-dep_RNA_pol. [Graphical view]
Pfam	PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_AMVLE

Accession

Primary (citable) accession number: P03593

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 1996
Last modified:	September 21, 2011
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program