Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Replicase large subunit

Gene
N/A
Organism
Tobacco mosaic virus (strain vulgare) (TMV) (Tobacco mosaic virus (strain U1))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Replicase large subunit: is an RNA-dependent RNA polymerase active in viral RNA replication.1 Publication
Replicase small subunit: is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential). It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation.Curated1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi833 – 8408ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Suppressor of RNA silencing, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase large subunit (EC:2.1.1.-, EC:2.7.7.-, EC:2.7.7.48, EC:3.6.4.13)
Alternative name(s):
183 kDa protein
RNA-directed RNA polymerase
Cleaved into the following chain:
Alternative name(s):
126 kDa protein
Methyltransferase/RNA helicase
Short name:
MT/HEL
OrganismiTobacco mosaic virus (strain vulgare) (TMV) (Tobacco mosaic virus (strain U1))
Taxonomic identifieri12243 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageVirgaviridaeTobamovirus
Virus hostiNicotiana tabacum (Common tobacco) [TaxID: 4097]
Proteomesi
  • UP000000522 Componenti: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi349 – 3491C → A: Loss of RNA silencing suppression activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16161616Replicase large subunitPRO_0000041162Add
BLAST
Chaini1 – 11161116Replicase small subunitPRO_0000041163Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Protein-protein interaction databases

BioGridi3509210. 1 interaction.
DIPiDIP-29269N.
IntActiP03586. 1 interaction.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 281210Alphavirus-like MTPROSITE-ProRule annotationAdd
BLAST
Domaini801 – 963163(+)RNA virus helicase ATP-bindingAdd
BLAST
Domaini964 – 1116153(+)RNA virus helicase C-terminalAdd
BLAST
Domaini1380 – 1493114RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 458409MethyltransferaseSequence analysisAdd
BLAST
Regioni830 – 1085256HelicaseSequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 alphavirus-like MT (methyltransferase) domain.PROSITE-ProRule annotation
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR002588. Alphavirus-like_MT_dom.
IPR027417. P-loop_NTPase.
IPR007094. RNA-dir_pol_PSvirus.
IPR001788. Tymovirus_RNA-dep_RNA_pol.
[Graphical view]
PfamiPF00978. RdRP_2. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF01660. Vmethyltransf. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51743. ALPHAVIRUS_MT. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03586-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYTQTATTS ALLDTVRGNN SLVNDLAKRR LYDTAVEEFN ARDRRPKVNF
60 70 80 90 100
SKVISEEQTL IATRAYPEFQ ITFYNTQNAV HSLAGGLRSL ELEYLMMQIP
110 120 130 140 150
YGSLTYDIGG NFASHLFKGR AYVHCCMPNL DVRDIMRHEG QKDSIELYLS
160 170 180 190 200
RLERGGKTVP NFQKEAFDRY AEIPEDAVCH NTFQTMRHQP MQQSGRVYAI
210 220 230 240 250
ALHSIYDIPA DEFGAALLRK NVHTCYAAFH FSENLLLEDS YVNLDEINAC
260 270 280 290 300
FSRDGDKLTF SFASESTLNY CHSYSNILKY VCKTYFPASN REVYMKEFLV
310 320 330 340 350
TRVNTWFCKF SRIDTFLLYK GVAHKSVDSE QFYTAMEDAW HYKKTLAMCN
360 370 380 390 400
SERILLEDSS SVNYWFPKMR DMVIVPLFDI SLETSKRTRK EVLVSKDFVF
410 420 430 440 450
TVLNHIRTYQ AKALTYANVL SFVESIRSRV IINGVTARSE WDVDKSLLQS
460 470 480 490 500
LSMTFYLHTK LAVLKDDLLI SKFSLGSKTV CQHVWDEISL AFGNAFPSVK
510 520 530 540 550
ERLLNRKLIR VAGDALEIRV PDLYVTFHDR LVTEYKASVD MPALDIRKKM
560 570 580 590 600
EETEVMYNAL SELSVLRESD KFDVDVFSQM CQSLEVDPMT AAKVIVAVMS
610 620 630 640 650
NESGLTLTFE RPTEANVALA LQDQEKASEG ALVVTSREVE EPSMKGSMAR
660 670 680 690 700
GELQLAGLAG DHPESSYSKN EEIESLEQFH MATADSLIRK QMSSIVYTGP
710 720 730 740 750
IKVQQMKNFI DSLVASLSAA VSNLVKILKD TAAIDLETRQ KFGVLDVASR
760 770 780 790 800
KWLIKPTAKS HAWGVVETHA RKYHVALLEY DEQGVVTCDD WRRVAVSSES
810 820 830 840 850
VVYSDMAKLR TLRRLLRNGE PHVSSAKVVL VDGVPGCGKT KEILSRVNFD
860 870 880 890 900
EDLILVPGKQ AAEMIRRRAN SSGIIVATKD NVKTVDSFMM NFGKSTRCQF
910 920 930 940 950
KRLFIDEGLM LHTGCVNFLV AMSLCEIAYV YGDTQQIPYI NRVSGFPYPA
960 970 980 990 1000
HFAKLEVDEV ETRRTTLRCP ADVTHYLNRR YEGFVMSTSS VKKSVSQEMV
1010 1020 1030 1040 1050
GGAAVINPIS KPLHGKILTF TQSDKEALLS RGYSDVHTVH EVQGETYSDV
1060 1070 1080 1090 1100
SLVRLTPTPV SIIAGDSPHV LVALSRHTCS LKYYTVVMDP LVSIIRDLEK
1110 1120 1130 1140 1150
LSSYLLDMYK VDAGTQXQLQ IDSVFKGSNL FVAAPKTGDI SDMQFYYDKC
1160 1170 1180 1190 1200
LPGNSTMMNN FDAVTMRLTD ISLNVKDCIL DMSKSVAAPK DQIKPLIPMV
1210 1220 1230 1240 1250
RTAAEMPRQT GLLENLVAMI KRNFNAPELS GIIDIENTAS LVVDKFFDSY
1260 1270 1280 1290 1300
LLKEKRKPNK NVSLFSRESL NRWLEKQEQV TIGQLADFDF VDLPAVDQYR
1310 1320 1330 1340 1350
HMYKAQPKQK LDTSIQTEYP ALQTIVYHSK KINAIFGPLF SELTRQLLDS
1360 1370 1380 1390 1400
VDSSRFLFFT RKTPAQIEDF FGDLDSHVPM DVLELDISKY DKSQNEFHCA
1410 1420 1430 1440 1450
VEYEIWRRLG FEDFLGEVWK QGHRKTTLKD YTAGIKTCIW YQRKSGDVTT
1460 1470 1480 1490 1500
FIGNTVIIAA CLASMLPMEK IIKGAFCGDD SLLYFPKGCE FPDVQHSANL
1510 1520 1530 1540 1550
MWNFEAKLFK KQYGYFCGRY VIHHDRGCIV YYDPLKLISK LGAKHIKDWE
1560 1570 1580 1590 1600
HLEEFRRSLC DVAVSLNNCA YYTQLDDAVW EVHKTAPPGS FVYKSLVKYL
1610
SDKVLFRSLF IDGSSC
Length:1,616
Mass (Da):183,468
Last modified:December 1, 2000 - v2
Checksum:i6DE2F2B11F5550CE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti9 – 91T → S.
Natural varianti14 – 141D → E.
Natural varianti21 – 211S → T.
Natural varianti37 – 371E → D.
Natural varianti638 – 6381E → A.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01408 Unassigned RNA. Translation: CAA24688.1.
V01409 Genomic RNA. No translation available.
PIRiA04194. WMTM18.
RefSeqiNP_056764.1. NC_001367.1.

Genome annotation databases

GeneIDi1494081.
KEGGivg:1494081.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01408 Unassigned RNA. Translation: CAA24688.1.
V01409 Genomic RNA. No translation available.
PIRiA04194. WMTM18.
RefSeqiNP_056764.1. NC_001367.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3509210. 1 interaction.
DIPiDIP-29269N.
IntActiP03586. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1494081.
KEGGivg:1494081.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR002588. Alphavirus-like_MT_dom.
IPR027417. P-loop_NTPase.
IPR007094. RNA-dir_pol_PSvirus.
IPR001788. Tymovirus_RNA-dep_RNA_pol.
[Graphical view]
PfamiPF00978. RdRP_2. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF01660. Vmethyltransf. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51743. ALPHAVIRUS_MT. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRDRP_TMV
AccessioniPrimary (citable) accession number: P03586
Secondary accession number(s): O41341
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 1, 2000
Last modified: July 6, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is translated as a fusion protein by episodic readthrough of a termination codon. When readthrough of the terminator codon TGA occurs between the codons for Gln-1116 and Gln-1118, this results in the addition of the RdRp region to the replicase.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.