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P03554

- POL_CAMVS

UniProt

P03554 - POL_CAMVS

Protein

Enzymatic polyprotein

Gene

ORF V

Organism
Cauliflower mosaic virus (strain Strasbourg) (CaMV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Encodes for at least two polypeptides: protease (PR) and reverse transcriptase (RT). The protease processes the polyprotein in cis. Reverse transcriptase is multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated, and reverse-transcribed inside the nucleocapsid. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA) By similarity.By similarity

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei45 – 451

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW
    2. endonuclease activity Source: UniProtKB-KW
    3. RNA binding Source: InterPro
    4. RNA-directed DNA polymerase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

    Protein family/group databases

    MEROPSiA03.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enzymatic polyprotein
    Including the following 3 domains:
    Aspartic protease (EC:3.4.23.-)
    Endonuclease
    Reverse transcriptase (EC:2.7.7.49)
    Gene namesi
    ORF Names:ORF V
    OrganismiCauliflower mosaic virus (strain Strasbourg) (CaMV)
    Taxonomic identifieri10648 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesCaulimoviridaeCaulimovirus
    Virus hostiArabidopsis thaliana (Mouse-ear cress) [TaxID: 3702]
    Brassica [TaxID: 3705]
    Raphanus [TaxID: 3725]
    ProteomesiUP000002501: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 679679Enzymatic polyproteinPRO_0000222054Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP03554.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini272 – 452181Reverse transcriptasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni40 – 13091ProteaseBy similarityAdd
    BLAST

    Domaini

    The polymerase/reverse transcriptase (RT) and ribonuclease H (RH) domains are structured in five subdomains: finger, palm, thumb, connection and RNase H. Within the palm subdomain, the 'primer grip' region is thought to be involved in the positioning of the primer terminus for accommodating the incoming nucleotide. The RH domain stabilizes the association of RT with primer-template By similarity.By similarity

    Sequence similaritiesi

    Contains 1 peptidase A3A domain.Curated
    Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR000588. Pept_A3A.
    IPR000477. RT_dom.
    [Graphical view]
    PfamiPF02160. Peptidase_A3. 1 hit.
    PF00078. RVT_1. 1 hit.
    [Graphical view]
    PRINTSiPR00731. CAULIMOPTASE.
    PROSITEiPS50878. RT_POL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03554-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDHLLLKTQT QTEQVMNVTN PNSIYIKGRL YFKGYKKIEL HCFVDTGASL    50
    CIASKFVIPE EHWVNAERPI MVKIADGSSI TISKVCKDID LIIAGEIFRI 100
    PTVYQQESGI DFIIGNNFCQ LYEPFIQFTD RVIFTKNKSY PVHIAKLTRA 150
    VRVGTEGFLE SMKKRSKTQQ PEPVNISTNK IENPLEEIAI LSEGRRLSEE 200
    KLFITQQRMQ KIEELLEKVC SENPLDPNKT KQWMKASIKL SDPSKAIKVK 250
    PMKYSPMDRE EFDKQIKELL DLKVIKPSKS PHMAPAFLVN NEAEKRRGKK 300
    RMVVNYKAMN KATVGDAYNL PNKDELLTLI RGKKIFSSFD CKSGFWQVLL 350
    DQESRPLTAF TCPQGHYEWN VVPFGLKQAP SIFQRHMDEA FRVFRKFCCV 400
    YVDDILVFSN NEEDHLLHVA MILQKCNQHG IILSKKKAQL FKKKINFLGL 450
    EIDEGTHKPQ GHILEHINKF PDTLEDKKQL QRFLGILTYA SDYIPKLAQI 500
    RKPLQAKLKE NVPWRWTKED TLYMQKVKKN LQGFPPLHHP LPEEKLIIET 550
    DASDDYWGGM LKAIKINEGT NTELICRYAS GSFKAAEKNY HSNDKETLAV 600
    INTIKKFSIY LTPVHFLIRT DNTHFKSFVN LNYKGDSKLG RNIRWQAWLS 650
    HYSFDVEHIK GTDNHFADFL SREFNKVNS 679
    Length:679
    Mass (Da):78,629
    Last modified:July 21, 1986 - v1
    Checksum:i9EE527BCD460B766
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00141 Genomic DNA. Translation: CAA23460.1.
    PIRiD90799. QQCV5.
    RefSeqiNP_056728.1. NC_001497.1.

    Genome annotation databases

    GeneIDi1489542.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00141 Genomic DNA. Translation: CAA23460.1 .
    PIRi D90799. QQCV5.
    RefSeqi NP_056728.1. NC_001497.1.

    3D structure databases

    ProteinModelPortali P03554.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi A03.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1489542.

    Family and domain databases

    InterProi IPR000588. Pept_A3A.
    IPR000477. RT_dom.
    [Graphical view ]
    Pfami PF02160. Peptidase_A3. 1 hit.
    PF00078. RVT_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00731. CAULIMOPTASE.
    PROSITEi PS50878. RT_POL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of cauliflower mosaic virus DNA."
      Franck A., Guilley H., Jonard G., Richards K., Hirth L.
      Cell 21:285-294(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiPOL_CAMVS
    AccessioniPrimary (citable) accession number: P03554
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3