P03554 (POL_CAMVS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enzymatic polyprotein

Including the following 3 domains:

1. Aspartic protease
   EC=3.4.23.-
2. Endonuclease
3. Reverse transcriptase
   EC=2.7.7.49

Gene names

ORF Names:

ORF V

Organism

Cauliflower mosaic virus (strain Strasbourg) (CaMV) [Reference proteome]

Taxonomic identifier

10648 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Caulimoviridae › Caulimovirus › Cauliflower mosaic virus

Virus host

Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702]
Brassica [TaxID: 3705]
Raphanus [TaxID: 3725]

Protein attributes

Sequence length	679 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Encodes for at least two polypeptides: protease (PR) and reverse transcriptase (RT). The protease processes the polyprotein in cis. Reverse transcriptase is multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated, and reverse-transcribed inside the nucleocapsid. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA) By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Domain	The polymerase/reverse transcriptase (RT) and ribonuclease H (RH) domains are structured in five subdomains: finger, palm, thumb, connection and RNase H. Within the palm subdomain, the 'primer grip' region is thought to be involved in the positioning of the primer terminus for accommodating the incoming nucleotide. The RH domain stabilizes the association of RT with primer-template By similarity.
Sequence similarities	Belongs to the caulimoviridae enzymatic polyprotein family. Contains 1 peptidase A3A domain. Contains 1 reverse transcriptase domain.

Ontologies

Keywords
Molecular function	Aspartyl protease Endonuclease Hydrolase Nuclease Nucleotidyltransferase Protease RNA-directed DNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 679	679	Enzymatic polyprotein		PRO_0000222054
Regions
	Domain	272 – 452	181	Reverse transcriptase
	Region	40 – 130	91	Protease By similarity
Sites
	Active site	45	1

Sequences

	Sequence		Length	Mass (Da)
	P03554 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 9EE527BCD460B766	FASTA	679	78,629
	10 20 30 40 50 60 MDHLLLKTQT QTEQVMNVTN PNSIYIKGRL YFKGYKKIEL HCFVDTGASL CIASKFVIPE 70 80 90 100 110 120 EHWVNAERPI MVKIADGSSI TISKVCKDID LIIAGEIFRI PTVYQQESGI DFIIGNNFCQ 130 140 150 160 170 180 LYEPFIQFTD RVIFTKNKSY PVHIAKLTRA VRVGTEGFLE SMKKRSKTQQ PEPVNISTNK 190 200 210 220 230 240 IENPLEEIAI LSEGRRLSEE KLFITQQRMQ KIEELLEKVC SENPLDPNKT KQWMKASIKL 250 260 270 280 290 300 SDPSKAIKVK PMKYSPMDRE EFDKQIKELL DLKVIKPSKS PHMAPAFLVN NEAEKRRGKK 310 320 330 340 350 360 RMVVNYKAMN KATVGDAYNL PNKDELLTLI RGKKIFSSFD CKSGFWQVLL DQESRPLTAF 370 380 390 400 410 420 TCPQGHYEWN VVPFGLKQAP SIFQRHMDEA FRVFRKFCCV YVDDILVFSN NEEDHLLHVA 430 440 450 460 470 480 MILQKCNQHG IILSKKKAQL FKKKINFLGL EIDEGTHKPQ GHILEHINKF PDTLEDKKQL 490 500 510 520 530 540 QRFLGILTYA SDYIPKLAQI RKPLQAKLKE NVPWRWTKED TLYMQKVKKN LQGFPPLHHP 550 560 570 580 590 600 LPEEKLIIET DASDDYWGGM LKAIKINEGT NTELICRYAS GSFKAAEKNY HSNDKETLAV 610 620 630 640 650 660 INTIKKFSIY LTPVHFLIRT DNTHFKSFVN LNYKGDSKLG RNIRWQAWLS HYSFDVEHIK 670 GTDNHFADFL SREFNKVNS

References

[1]	"Nucleotide sequence of cauliflower mosaic virus DNA." Franck A., Guilley H., Jonard G., Richards K., Hirth L. Cell 21:285-294(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00141 Genomic DNA. Translation: CAA23460.1.
PIR	QQCV5. D90799.
RefSeq	NP_056728.1. NC_001497.1.
3D structure databases
ProteinModelPortal	P03554.
ModBase	Search...
Protein family/group databases
MEROPS	A03.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1489542.
Family and domain databases
InterPro	IPR000588. Pept_A3A. IPR000477. RVT. [Graphical view]
Pfam	PF02160. Peptidase_A3. 1 hit. PF00078. RVT_1. 1 hit. [Graphical view]
PRINTS	PR00731. CAULIMOPTASE.
PROSITE	PS00141. ASP_PROTEASE. False negative. PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POL_CAMVS

Accession

Primary (citable) accession number: P03554

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program