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P03554

- POL_CAMVS

UniProt

P03554 - POL_CAMVS

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Protein
Enzymatic polyprotein
Gene
ORF V
Organism
Cauliflower mosaic virus (strain Strasbourg) (CaMV)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Encodes for at least two polypeptides: protease (PR) and reverse transcriptase (RT). The protease processes the polyprotein in cis. Reverse transcriptase is multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated, and reverse-transcribed inside the nucleocapsid. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA) By similarity.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. RNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. aspartic-type endopeptidase activity Source: UniProtKB-KW
  4. endonuclease activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Protein family/group databases

MEROPSiA03.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Enzymatic polyprotein
Including the following 3 domains:
Aspartic protease (EC:3.4.23.-)
Endonuclease
Reverse transcriptase (EC:2.7.7.49)
Gene namesi
ORF Names:ORF V
OrganismiCauliflower mosaic virus (strain Strasbourg) (CaMV)
Taxonomic identifieri10648 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesCaulimoviridaeCaulimovirus
Virus hostiArabidopsis thaliana (Mouse-ear cress) [TaxID: 3702]
Brassica [TaxID: 3705]
Raphanus [TaxID: 3725]
ProteomesiUP000002501: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 679679Enzymatic polyprotein
PRO_0000222054Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP03554.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini272 – 452181Reverse transcriptase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni40 – 13091Protease By similarity
Add
BLAST

Domaini

The polymerase/reverse transcriptase (RT) and ribonuclease H (RH) domains are structured in five subdomains: finger, palm, thumb, connection and RNase H. Within the palm subdomain, the 'primer grip' region is thought to be involved in the positioning of the primer terminus for accommodating the incoming nucleotide. The RH domain stabilizes the association of RT with primer-template By similarity.

Sequence similaritiesi

Family and domain databases

InterProiIPR000588. Pept_A3A.
IPR000477. RT_dom.
[Graphical view]
PfamiPF02160. Peptidase_A3. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
PRINTSiPR00731. CAULIMOPTASE.
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03554-1 [UniParc]FASTAAdd to Basket

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MDHLLLKTQT QTEQVMNVTN PNSIYIKGRL YFKGYKKIEL HCFVDTGASL    50
CIASKFVIPE EHWVNAERPI MVKIADGSSI TISKVCKDID LIIAGEIFRI 100
PTVYQQESGI DFIIGNNFCQ LYEPFIQFTD RVIFTKNKSY PVHIAKLTRA 150
VRVGTEGFLE SMKKRSKTQQ PEPVNISTNK IENPLEEIAI LSEGRRLSEE 200
KLFITQQRMQ KIEELLEKVC SENPLDPNKT KQWMKASIKL SDPSKAIKVK 250
PMKYSPMDRE EFDKQIKELL DLKVIKPSKS PHMAPAFLVN NEAEKRRGKK 300
RMVVNYKAMN KATVGDAYNL PNKDELLTLI RGKKIFSSFD CKSGFWQVLL 350
DQESRPLTAF TCPQGHYEWN VVPFGLKQAP SIFQRHMDEA FRVFRKFCCV 400
YVDDILVFSN NEEDHLLHVA MILQKCNQHG IILSKKKAQL FKKKINFLGL 450
EIDEGTHKPQ GHILEHINKF PDTLEDKKQL QRFLGILTYA SDYIPKLAQI 500
RKPLQAKLKE NVPWRWTKED TLYMQKVKKN LQGFPPLHHP LPEEKLIIET 550
DASDDYWGGM LKAIKINEGT NTELICRYAS GSFKAAEKNY HSNDKETLAV 600
INTIKKFSIY LTPVHFLIRT DNTHFKSFVN LNYKGDSKLG RNIRWQAWLS 650
HYSFDVEHIK GTDNHFADFL SREFNKVNS 679
Length:679
Mass (Da):78,629
Last modified:July 21, 1986 - v1
Checksum:i9EE527BCD460B766
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V00141 Genomic DNA. Translation: CAA23460.1.
PIRiD90799. QQCV5.
RefSeqiNP_056728.1. NC_001497.1.

Genome annotation databases

GeneIDi1489542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V00141 Genomic DNA. Translation: CAA23460.1 .
PIRi D90799. QQCV5.
RefSeqi NP_056728.1. NC_001497.1.

3D structure databases

ProteinModelPortali P03554.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi A03.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1489542.

Family and domain databases

InterProi IPR000588. Pept_A3A.
IPR000477. RT_dom.
[Graphical view ]
Pfami PF02160. Peptidase_A3. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view ]
PRINTSi PR00731. CAULIMOPTASE.
PROSITEi PS50878. RT_POL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of cauliflower mosaic virus DNA."
    Franck A., Guilley H., Jonard G., Richards K., Hirth L.
    Cell 21:285-294(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPOL_CAMVS
AccessioniPrimary (citable) accession number: P03554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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