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P03554 (POL_CAMVS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Enzymatic polyprotein

Including the following 3 domains:

  1. Aspartic protease
    EC=3.4.23.-
  2. Endonuclease
  3. Reverse transcriptase
    EC=2.7.7.49
Gene names
ORF Names:ORF V
OrganismCauliflower mosaic virus (strain Strasbourg) (CaMV) [Reference proteome]
Taxonomic identifier10648 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesCaulimoviridaeCaulimovirus
Virus hostArabidopsis thaliana (Mouse-ear cress) [TaxID: 3702]
Brassica [TaxID: 3705]
Raphanus [TaxID: 3725]

Protein attributes

Sequence length679 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Encodes for at least two polypeptides: protease (PR) and reverse transcriptase (RT). The protease processes the polyprotein in cis. Reverse transcriptase is multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated, and reverse-transcribed inside the nucleocapsid. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA) By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Domain

The polymerase/reverse transcriptase (RT) and ribonuclease H (RH) domains are structured in five subdomains: finger, palm, thumb, connection and RNase H. Within the palm subdomain, the 'primer grip' region is thought to be involved in the positioning of the primer terminus for accommodating the incoming nucleotide. The RH domain stabilizes the association of RT with primer-template By similarity.

Sequence similarities

Belongs to the caulimoviridae enzymatic polyprotein family.

Contains 1 peptidase A3A domain.

Contains 1 reverse transcriptase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 679679Enzymatic polyprotein
PRO_0000222054

Regions

Domain272 – 452181Reverse transcriptase
Region40 – 13091Protease By similarity

Sites

Active site451

Sequences

Sequence LengthMass (Da)Tools
P03554 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 9EE527BCD460B766

FASTA67978,629
        10         20         30         40         50         60 
MDHLLLKTQT QTEQVMNVTN PNSIYIKGRL YFKGYKKIEL HCFVDTGASL CIASKFVIPE 

        70         80         90        100        110        120 
EHWVNAERPI MVKIADGSSI TISKVCKDID LIIAGEIFRI PTVYQQESGI DFIIGNNFCQ 

       130        140        150        160        170        180 
LYEPFIQFTD RVIFTKNKSY PVHIAKLTRA VRVGTEGFLE SMKKRSKTQQ PEPVNISTNK 

       190        200        210        220        230        240 
IENPLEEIAI LSEGRRLSEE KLFITQQRMQ KIEELLEKVC SENPLDPNKT KQWMKASIKL 

       250        260        270        280        290        300 
SDPSKAIKVK PMKYSPMDRE EFDKQIKELL DLKVIKPSKS PHMAPAFLVN NEAEKRRGKK 

       310        320        330        340        350        360 
RMVVNYKAMN KATVGDAYNL PNKDELLTLI RGKKIFSSFD CKSGFWQVLL DQESRPLTAF 

       370        380        390        400        410        420 
TCPQGHYEWN VVPFGLKQAP SIFQRHMDEA FRVFRKFCCV YVDDILVFSN NEEDHLLHVA 

       430        440        450        460        470        480 
MILQKCNQHG IILSKKKAQL FKKKINFLGL EIDEGTHKPQ GHILEHINKF PDTLEDKKQL 

       490        500        510        520        530        540 
QRFLGILTYA SDYIPKLAQI RKPLQAKLKE NVPWRWTKED TLYMQKVKKN LQGFPPLHHP 

       550        560        570        580        590        600 
LPEEKLIIET DASDDYWGGM LKAIKINEGT NTELICRYAS GSFKAAEKNY HSNDKETLAV 

       610        620        630        640        650        660 
INTIKKFSIY LTPVHFLIRT DNTHFKSFVN LNYKGDSKLG RNIRWQAWLS HYSFDVEHIK 

       670 
GTDNHFADFL SREFNKVNS 

« Hide

References

[1]"Nucleotide sequence of cauliflower mosaic virus DNA."
Franck A., Guilley H., Jonard G., Richards K., Hirth L.
Cell 21:285-294(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00141 Genomic DNA. Translation: CAA23460.1.
PIRQQCV5. D90799.
RefSeqNP_056728.1. NC_001497.1.

3D structure databases

ProteinModelPortalP03554.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSA03.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1489542.

Family and domain databases

InterProIPR000588. Pept_A3A.
IPR000477. RT_dom.
[Graphical view]
PfamPF02160. Peptidase_A3. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
PRINTSPR00731. CAULIMOPTASE.
PROSITEPS50878. RT_POL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOL_CAMVS
AccessionPrimary (citable) accession number: P03554
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries