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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus A (strain RVA/SA11-Both/G3P5B[2]) (RV-A) (Simian Agent 11 (strain Both))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Magnesium is required for NTPase activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi153MagnesiumSequence analysis1
Metal bindingi171MagnesiumSequence analysis1
Active sitei225For NTPase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, RNA-binding
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name:
NS35
OrganismiRotavirus A (strain RVA/SA11-Both/G3P5B[2]) (RV-A) (Simian Agent 11 (strain Both))
Taxonomic identifieri37137 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]
Proteomesi
  • UP000007180 Componenti: Genome

Subcellular locationi

  • Host cytoplasm Curated
  • Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001495501 – 317Non-structural protein 2Add BLAST317

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP03537.
SMRiP03537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 241RNA-bindingSequence analysisAdd BLAST37

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.Curated

Phylogenomic databases

OrthoDBiVOG090000GH.

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiView protein in InterPro
IPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
PfamiView protein in Pfam
PF02509. Rota_NS35. 1 hit.
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.

Sequencei

Sequence statusi: Complete.

P03537-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELACFCYP HLENDSYRFI PFNSLAIKCM LTAKVDKKDQ DKFYNSIIYG
60 70 80 90 100
IAPPPQFKKR YNTSDNSRGM NYETSMFNKV AALICEALNS IKVTQSDVAS
110 120 130 140 150
VLSKIVSVRH LENLVLRREN HQDVLFHSKE LLLKSVLIAI GHSKEIETTA
160 170 180 190 200
TAEGGEIVFQ NAAFTMWKLT YLEHKLMPIL DQNFIEYKIT LNEDKPISES
210 220 230 240 250
HVKELIAELR WQYNKFAVIT HGKGHYRVVK YSSVANHADR VYATFKSNNK
260 270 280 290 300
NGNMIEFNLL DQRIIWQNWY AFTSSMKQGN TLEICKKLLF QKMKRESNPF
310
KGLSTDRKMD EVSQIGI
Length:317
Mass (Da):36,628
Last modified:July 21, 1986 - v1
Checksum:i25670C29E6607474
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02353 Genomic RNA. Translation: AAA47293.1.
PIRiA04144. WMXR3S.

Similar proteinsi

Entry informationi

Entry nameiNSP2_ROTS1
AccessioniPrimary (citable) accession number: P03537
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 27, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families