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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain RVA/SA11-Both/G3P5B[2]) (RV-A) (Simian Agent 11 (strain Both))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in stimulating the translation of viral mRNAs. These mRNAs are capped but not polyadenylated, instead terminating in a conserved sequence 'GACC' at the 3' that is recognized by NSP3, which competes with host PABPC1 for EIF4G1 binding. The interaction between NSP3 and host EIF4G1 stabilizes the EIF4E-EIF4G1 interaction, thereby facilitating the initiation of capped mRNA translation.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3UniRule annotation
Short name:
NSP3UniRule annotation
Alternative name(s):
NCVP4UniRule annotation
Non-structural RNA-binding protein 34UniRule annotation
Short name:
NS34UniRule annotation
OrganismiRotavirus A (strain RVA/SA11-Both/G3P5B[2]) (RV-A) (Simian Agent 11 (strain Both))
Taxonomic identifieri37137 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]
Proteomesi
  • UP000007180 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001495411 – 315Non-structural protein 3Add BLAST315

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1.UniRule annotation

Protein-protein interaction databases

IntActiP03536, 1 interactor

Structurei

Secondary structure

1315
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 32Combined sources25
Helixi39 – 56Combined sources18
Helixi61 – 74Combined sources14
Helixi79 – 89Combined sources11
Helixi93 – 107Combined sources15
Helixi114 – 123Combined sources10
Beta strandi125 – 127Combined sources3
Beta strandi136 – 138Combined sources3
Helixi141 – 147Combined sources7
Beta strandi152 – 155Combined sources4
Helixi156 – 161Combined sources6
Helixi209 – 211Combined sources3
Helixi212 – 250Combined sources39
Helixi256 – 266Combined sources11
Helixi274 – 303Combined sources30
Turni304 – 307Combined sources4
Beta strandi309 – 311Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KNZX-ray2.45A/B/C/D/I/J/M/N4-164[»]
1LJ2X-ray2.38A/B206-315[»]
ProteinModelPortaliP03536
SMRiP03536
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03536

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 149RNA-bindingUniRule annotationAdd BLAST149
Regioni150 – 206DimerizationUniRule annotationAdd BLAST57
Regioni170 – 234Interaction with host ZC3H7BUniRule annotationAdd BLAST65
Regioni208 – 315Interaction with host EIF4G1UniRule annotationAdd BLAST108

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili166 – 237UniRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the rotavirus NSP3 family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

OrthoDBiVOG090000G7

Family and domain databases

HAMAPiMF_04094 ROTA_A_NSP3, 1 hit
MF_04090 ROTA_NSP3, 1 hit
InterProiView protein in InterPro
IPR036082 NSP3_sf
IPR002873 Rotavirus_NSP3
PfamiView protein in Pfam
PF01665 Rota_NSP3, 1 hit
SUPFAMiSSF69903 SSF69903, 1 hit

Sequencei

Sequence statusi: Complete.

P03536-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKMESTQQM AVSIINSSFE AAVVAATSAL ENMGIEYDYQ DIYSRVKNKF
60 70 80 90 100
DFVMDDSGVK NNPIGKAITI DQALNNKFGS AIRNRNWLAD TSRPAKLDED
110 120 130 140 150
VNKLRMMLSS KGIDQKMRVL NACFSVKRIP GKSSSIIKCT KLMRDKLERG
160 170 180 190 200
EVEVDDSFVD EKMEVDTIDW KSRYEQLEQR FESLKSRVNE KYNNWVLKAR
210 220 230 240 250
KMNENMHSLQ NVIPQQQAHI AELQVYNNKL ERDLQNKIGS LTSSIEWYLR
260 270 280 290 300
SMELDPEIKA DIEQQINSID AINPLHAFDD LESVIRNLIS DYDKLFLMFK
310
GLIQRCNYQY SFGCE
Length:315
Mass (Da):36,445
Last modified:July 21, 1986 - v1
Checksum:i06BE050DDE9218E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00355 Genomic RNA Translation: CAA25102.1
PIRiA04142 MNXR4S

Similar proteinsi

Entry informationi

Entry nameiNSP3_ROTS1
AccessioniPrimary (citable) accession number: P03536
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 25, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health