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P03536

- NSP3_ROTS1

UniProt

P03536 - NSP3_ROTS1

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Protein
Non-structural protein 3
Gene
N/A
Organism
Rotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) (Simian Agent 11 (strain Both))
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) (Simian Agent 11 (strain Both))
Taxonomic identifieri37137 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]
ProteomesiUP000007180: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Non-structural protein 3
PRO_0000149541Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Protein-protein interaction databases

IntActiP03536. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 3225
Helixi39 – 5618
Helixi61 – 7414
Helixi79 – 8911
Helixi93 – 10715
Helixi114 – 12310
Beta strandi125 – 1273
Beta strandi136 – 1383
Helixi141 – 1477
Beta strandi152 – 1554
Helixi156 – 1616
Helixi209 – 2113
Helixi212 – 25039
Helixi256 – 26611
Helixi274 – 30330
Turni304 – 3074
Beta strandi309 – 3113

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KNZX-ray2.45A/B/C/D/I/J/M/N4-164[»]
1LJ2X-ray2.38A/B206-315[»]
ProteinModelPortaliP03536.
SMRiP03536. Positions 7-163, 206-315.

Miscellaneous databases

EvolutionaryTraceiP03536.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 149146RNA-binding
Add
BLAST
Regioni150 – 20657Dimerization
Add
BLAST
Regioni170 – 23465Interaction with ZC3H7B By similarity
Add
BLAST
Regioni208 – 315108Interaction with EIF4G1
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili166 – 23772 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

P03536-1 [UniParc]FASTAAdd to Basket

« Hide

MLKMESTQQM AVSIINSSFE AAVVAATSAL ENMGIEYDYQ DIYSRVKNKF    50
DFVMDDSGVK NNPIGKAITI DQALNNKFGS AIRNRNWLAD TSRPAKLDED 100
VNKLRMMLSS KGIDQKMRVL NACFSVKRIP GKSSSIIKCT KLMRDKLERG 150
EVEVDDSFVD EKMEVDTIDW KSRYEQLEQR FESLKSRVNE KYNNWVLKAR 200
KMNENMHSLQ NVIPQQQAHI AELQVYNNKL ERDLQNKIGS LTSSIEWYLR 250
SMELDPEIKA DIEQQINSID AINPLHAFDD LESVIRNLIS DYDKLFLMFK 300
GLIQRCNYQY SFGCE 315
Length:315
Mass (Da):36,445
Last modified:July 21, 1986 - v1
Checksum:i06BE050DDE9218E9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00355 Genomic RNA. Translation: CAA25102.1.
PIRiA04142. MNXR4S.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00355 Genomic RNA. Translation: CAA25102.1 .
PIRi A04142. MNXR4S.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KNZ X-ray 2.45 A/B/C/D/I/J/M/N 4-164 [» ]
1LJ2 X-ray 2.38 A/B 206-315 [» ]
ProteinModelPortali P03536.
SMRi P03536. Positions 7-163, 206-315.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P03536. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P03536.

Family and domain databases

InterProi IPR002873. Rotavirus_NSP3.
[Graphical view ]
Pfami PF01665. Rota_NSP3. 1 hit.
[Graphical view ]
SUPFAMi SSF69903. SSF69903. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the dsRNA genomic segment 7 of Simian 11 rotavirus."
    Both G.W., Bellamy A.R., Siegman L.J.
    Nucleic Acids Res. 12:1621-1626(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer."
    Deo R.C., Groft C.M., Rajashankar K.R., Burley S.K.
    Cell 108:71-81(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 4-164, RNA-BINDING.
  3. "Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization."
    Groft C.M., Burley S.K.
    Mol. Cell 9:1273-1283(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) OF 206-315.

Entry informationi

Entry nameiNSP3_ROTS1
AccessioniPrimary (citable) accession number: P03536
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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