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P03536

- NSP3_ROTS1

UniProt

P03536 - NSP3_ROTS1

Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) (Simian Agent 11 (strain Both))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of translation Source: UniProtKB-KW
    2. suppression by virus of host gene expression Source: UniProtKB-KW

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 3
    Short name:
    NSP3
    Alternative name(s):
    NCVP4
    Non-structural RNA-binding protein 34
    Short name:
    NS34
    OrganismiRotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) (Simian Agent 11 (strain Both))
    Taxonomic identifieri37137 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]
    ProteomesiUP000007180: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315Non-structural protein 3PRO_0000149541Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP03536. 1 interaction.

    Structurei

    Secondary structure

    1
    315
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 3225
    Helixi39 – 5618
    Helixi61 – 7414
    Helixi79 – 8911
    Helixi93 – 10715
    Helixi114 – 12310
    Beta strandi125 – 1273
    Beta strandi136 – 1383
    Helixi141 – 1477
    Beta strandi152 – 1554
    Helixi156 – 1616
    Helixi209 – 2113
    Helixi212 – 25039
    Helixi256 – 26611
    Helixi274 – 30330
    Turni304 – 3074
    Beta strandi309 – 3113

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KNZX-ray2.45A/B/C/D/I/J/M/N4-164[»]
    1LJ2X-ray2.38A/B206-315[»]
    ProteinModelPortaliP03536.
    SMRiP03536. Positions 7-163, 206-315.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03536.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni4 – 149146RNA-bindingAdd
    BLAST
    Regioni150 – 20657DimerizationAdd
    BLAST
    Regioni170 – 23465Interaction with ZC3H7BBy similarityAdd
    BLAST
    Regioni208 – 315108Interaction with EIF4G1Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili166 – 23772Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus A NSP3 family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR002873. Rotavirus_NSP3.
    [Graphical view]
    PfamiPF01665. Rota_NSP3. 1 hit.
    [Graphical view]
    SUPFAMiSSF69903. SSF69903. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P03536-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKMESTQQM AVSIINSSFE AAVVAATSAL ENMGIEYDYQ DIYSRVKNKF    50
    DFVMDDSGVK NNPIGKAITI DQALNNKFGS AIRNRNWLAD TSRPAKLDED 100
    VNKLRMMLSS KGIDQKMRVL NACFSVKRIP GKSSSIIKCT KLMRDKLERG 150
    EVEVDDSFVD EKMEVDTIDW KSRYEQLEQR FESLKSRVNE KYNNWVLKAR 200
    KMNENMHSLQ NVIPQQQAHI AELQVYNNKL ERDLQNKIGS LTSSIEWYLR 250
    SMELDPEIKA DIEQQINSID AINPLHAFDD LESVIRNLIS DYDKLFLMFK 300
    GLIQRCNYQY SFGCE 315
    Length:315
    Mass (Da):36,445
    Last modified:July 21, 1986 - v1
    Checksum:i06BE050DDE9218E9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00355 Genomic RNA. Translation: CAA25102.1.
    PIRiA04142. MNXR4S.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00355 Genomic RNA. Translation: CAA25102.1 .
    PIRi A04142. MNXR4S.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KNZ X-ray 2.45 A/B/C/D/I/J/M/N 4-164 [» ]
    1LJ2 X-ray 2.38 A/B 206-315 [» ]
    ProteinModelPortali P03536.
    SMRi P03536. Positions 7-163, 206-315.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P03536. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P03536.

    Family and domain databases

    InterProi IPR002873. Rotavirus_NSP3.
    [Graphical view ]
    Pfami PF01665. Rota_NSP3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69903. SSF69903. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the dsRNA genomic segment 7 of Simian 11 rotavirus."
      Both G.W., Bellamy A.R., Siegman L.J.
      Nucleic Acids Res. 12:1621-1626(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer."
      Deo R.C., Groft C.M., Rajashankar K.R., Burley S.K.
      Cell 108:71-81(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 4-164, RNA-BINDING.
    3. "Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization."
      Groft C.M., Burley S.K.
      Mol. Cell 9:1273-1283(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) OF 206-315.

    Entry informationi

    Entry nameiNSP3_ROTS1
    AccessioniPrimary (citable) accession number: P03536
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3