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P03533

- VP7_ROTS1

UniProt

P03533 - VP7_ROTS1

Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) (Simian Agent 11 (strain Both))
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi127 – 15731Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid glycoprotein VP7
    OrganismiRotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) (Simian Agent 11 (strain Both))
    Taxonomic identifieri37137 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]
    ProteomesiUP000007180: Genome

    Subcellular locationi

    Virion. Host endoplasmic reticulum lumen Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles.

    GO - Cellular componenti

    1. host cell endoplasmic reticulum lumen Source: UniProtKB-SubCell
    2. T=13 icosahedral viral capsid Source: UniProtKB-KW
    3. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 5050Sequence AnalysisAdd
    BLAST
    Chaini51 – 326276Outer capsid glycoprotein VP7Sequence AnalysisPRO_0000149621Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi69 – 691N-linked (GlcNAc...) (high mannose); by host1 Publication

    Post-translational modificationi

    The N-terminus is blocked possibly by pyroglutamic acid.
    N-glycosylated.1 Publication
    Intramolecular disulfide bonds.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homotrimer; in the presence of calcium By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3.By similarity1 Publication

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi253 – 2553GPR motif; interaction with ITGAX/ITGB2 heterodimerCurated

    Sequence similaritiesi

    Belongs to the rotavirus VP7 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR001963. VP7.
    [Graphical view]
    PfamiPF00434. VP7. 1 hit.
    [Graphical view]
    ProDomiPD000191. VP7. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform 1 (identifier: P03533-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MYGIEYTTVL TFLISIILLN YILKSLTRIM DCIIYRLLFI IVILSPFLRA    50
    QNYGINLPIT GSMDTAYANS TQEETFLTST LCLYYPTEAA TEINDNSWKD 100
    TLSQLFLTKG WPTGSVYFKE YTNIASFSVD PQLYCDYNVV LMKYDATLQL 150
    DMSELADLIL NEWLCNPMDI TLYYYQQTDE ANKWISMGSS CTIKVCPLNT 200
    QTLGIGCLTT DATTFEEVAT AEKLVITDVV DGVNHKLDVT TATCTIRNCK 250
    KLGPRENVAV IQVGGSDILD ITADPTTAPQ TERMMRINWK KWWQVFYTVV 300
    DYVDQIIQVM SKRSRSLNSA AFYYRV 326
    Length:326
    Mass (Da):37,120
    Last modified:July 21, 1986 - v1
    Checksum:iC75549FD1F2A0C31
    GO
    Isoform 2 (identifier: P03533-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-29: Missing.

    Note: Produced by alternative initiation at Met-30 of isoform 1. This isoform is thought to be the major form in the cell.

    Show »
    Length:297
    Mass (Da):33,732
    Checksum:i91AE8498DCF78BF3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161I → T in AAA47307. (PubMed:6323768)Curated
    Sequence conflicti32 – 321C → F in AAA47307. (PubMed:6323768)Curated
    Sequence conflicti37 – 371L → F in AAA47307. (PubMed:6323768)Curated
    Sequence conflicti60 – 601T → A in AAA47307. (PubMed:6323768)Curated
    Sequence conflicti75 – 751T → P in AAA47307. (PubMed:6323768)Curated
    Sequence conflicti114 – 1141G → E in AAA47307. (PubMed:6323768)Curated
    Sequence conflicti219 – 2191A → P in AAA47307. (PubMed:6323768)Curated
    Sequence conflicti269 – 2691L → H in CAA24788. (PubMed:6304692)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2929Missing in isoform 2. CuratedVSP_036750Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01190 Unassigned RNA. Translation: CAA24510.1.
    V01546 Genomic RNA. Translation: CAA24788.1.
    K02028 Genomic RNA. Translation: AAA47307.1.
    PIRiA04135. VGXR1S.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01190 Unassigned RNA. Translation: CAA24510.1 .
    V01546 Genomic RNA. Translation: CAA24788.1 .
    K02028 Genomic RNA. Translation: AAA47307.1 .
    PIRi A04135. VGXR1S.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001963. VP7.
    [Graphical view ]
    Pfami PF00434. VP7. 1 hit.
    [Graphical view ]
    ProDomi PD000191. VP7. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence."
      Both G.W., Mattick J.S., Bellamy A.R.
      Proc. Natl. Acad. Sci. U.S.A. 80:3091-3095(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Primary structure of the neutralization antigen of simian rotavirus SA11 as deduced from cDNA sequence."
      Arias C.F., Lopez S., Bell J.R., Strauss J.H.
      J. Virol. 50:657-661(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Processing of the rough endoplasmic reticulum membrane glycoproteins of rotavirus SA11."
      Kabcenell A.K., Atkinson P.H.
      J. Cell Biol. 101:1270-1280(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION.
    4. "Processing of rotavirus glycoprotein VP7: implications for the retention of the protein in the endoplasmic reticulum."
      Stirzaker S.C., Whitfeld P.L., Christie D.L., Bellamy A.R., Both G.W.
      J. Cell Biol. 105:2897-2903(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIGNAL SEQUENCE CLEAVAGE SITE.
    5. "Integrin-using rotaviruses bind alpha2beta1 integrin alpha2 I domain via VP4 DGE sequence and recognize alphaXbeta2 and alphaVbeta3 by using VP7 during cell entry."
      Graham K.L., Halasz P., Tan Y., Hewish M.J., Takada Y., Mackow E.R., Robinson M.K., Coulson B.S.
      J. Virol. 77:9969-9978(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN INTEGRIN HETERODIMER ITGAX/ITGB2, INTERACTION WITH INTEGRIN HETERODIMER ITGA5/ITGB3.

    Entry informationi

    Entry nameiVP7_ROTS1
    AccessioniPrimary (citable) accession number: P03533
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In group A rotaviruses, VP7 defines the G serotype.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3