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P03533

- VP7_ROTS1

UniProt

P03533 - VP7_ROTS1

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Protein
Outer capsid glycoprotein VP7
Gene
N/A
Organism
Rotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) (Simian Agent 11 (strain Both))
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi127 – 15731 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid glycoprotein VP7
OrganismiRotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) (Simian Agent 11 (strain Both))
Taxonomic identifieri37137 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]
ProteomesiUP000007180: Genome

Subcellular locationi

Virion. Host endoplasmic reticulum lumen Reviewed prediction
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles.

GO - Cellular componenti

  1. T=13 icosahedral viral capsid Source: UniProtKB-KW
  2. host cell endoplasmic reticulum lumen Source: UniProtKB-SubCell
  3. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5050 Reviewed prediction
Add
BLAST
Chaini51 – 326276Outer capsid glycoprotein VP7 Reviewed prediction
PRO_0000149621Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...) (high mannose); by host Inferred

Post-translational modificationi

The N-terminus is blocked possibly by pyroglutamic acid.
N-glycosylated.1 Publication
Intramolecular disulfide bonds By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; in the presence of calcium By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3.1 Publication

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi253 – 2553GPR motif; interaction with ITGAX/ITGB2 heterodimer Inferred

Sequence similaritiesi

Belongs to the rotavirus VP7 family.

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001963. VP7.
[Graphical view]
PfamiPF00434. VP7. 1 hit.
[Graphical view]
ProDomiPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform 1 (identifier: P03533-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MYGIEYTTVL TFLISIILLN YILKSLTRIM DCIIYRLLFI IVILSPFLRA    50
QNYGINLPIT GSMDTAYANS TQEETFLTST LCLYYPTEAA TEINDNSWKD 100
TLSQLFLTKG WPTGSVYFKE YTNIASFSVD PQLYCDYNVV LMKYDATLQL 150
DMSELADLIL NEWLCNPMDI TLYYYQQTDE ANKWISMGSS CTIKVCPLNT 200
QTLGIGCLTT DATTFEEVAT AEKLVITDVV DGVNHKLDVT TATCTIRNCK 250
KLGPRENVAV IQVGGSDILD ITADPTTAPQ TERMMRINWK KWWQVFYTVV 300
DYVDQIIQVM SKRSRSLNSA AFYYRV 326
Length:326
Mass (Da):37,120
Last modified:July 21, 1986 - v1
Checksum:iC75549FD1F2A0C31
GO
Isoform 2 (identifier: P03533-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Note: Produced by alternative initiation at Met-30 of isoform 1. This isoform is thought to be the major form in the cell.

Show »
Length:297
Mass (Da):33,732
Checksum:i91AE8498DCF78BF3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2929Missing in isoform 2.
VSP_036750Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161I → T in AAA47307. 1 Publication
Sequence conflicti32 – 321C → F in AAA47307. 1 Publication
Sequence conflicti37 – 371L → F in AAA47307. 1 Publication
Sequence conflicti60 – 601T → A in AAA47307. 1 Publication
Sequence conflicti75 – 751T → P in AAA47307. 1 Publication
Sequence conflicti114 – 1141G → E in AAA47307. 1 Publication
Sequence conflicti219 – 2191A → P in AAA47307. 1 Publication
Sequence conflicti269 – 2691L → H in CAA24788. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01190 Unassigned RNA. Translation: CAA24510.1.
V01546 Genomic RNA. Translation: CAA24788.1.
K02028 Genomic RNA. Translation: AAA47307.1.
PIRiA04135. VGXR1S.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01190 Unassigned RNA. Translation: CAA24510.1 .
V01546 Genomic RNA. Translation: CAA24788.1 .
K02028 Genomic RNA. Translation: AAA47307.1 .
PIRi A04135. VGXR1S.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001963. VP7.
[Graphical view ]
Pfami PF00434. VP7. 1 hit.
[Graphical view ]
ProDomi PD000191. VP7. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence."
    Both G.W., Mattick J.S., Bellamy A.R.
    Proc. Natl. Acad. Sci. U.S.A. 80:3091-3095(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Primary structure of the neutralization antigen of simian rotavirus SA11 as deduced from cDNA sequence."
    Arias C.F., Lopez S., Bell J.R., Strauss J.H.
    J. Virol. 50:657-661(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Processing of the rough endoplasmic reticulum membrane glycoproteins of rotavirus SA11."
    Kabcenell A.K., Atkinson P.H.
    J. Cell Biol. 101:1270-1280(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.
  4. "Processing of rotavirus glycoprotein VP7: implications for the retention of the protein in the endoplasmic reticulum."
    Stirzaker S.C., Whitfeld P.L., Christie D.L., Bellamy A.R., Both G.W.
    J. Cell Biol. 105:2897-2903(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIGNAL SEQUENCE CLEAVAGE SITE.
  5. "Integrin-using rotaviruses bind alpha2beta1 integrin alpha2 I domain via VP4 DGE sequence and recognize alphaXbeta2 and alphaVbeta3 by using VP7 during cell entry."
    Graham K.L., Halasz P., Tan Y., Hewish M.J., Takada Y., Mackow E.R., Robinson M.K., Coulson B.S.
    J. Virol. 77:9969-9978(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN INTEGRIN HETERODIMER ITGAX/ITGB2, INTERACTION WITH INTEGRIN HETERODIMER ITGA5/ITGB3.

Entry informationi

Entry nameiVP7_ROTS1
AccessioniPrimary (citable) accession number: P03533
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi